TY  - JOUR
AU  - Dabetić, Nevena
AU  - Todorović, Vanja
AU  - Panić, Manuela
AU  - Radojčić Redovniković, Ivana
AU  - Šobajić, Slađana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3638
AB  - In the past few years, research efforts have focused on plant exploitation for deriving some valuable compounds. Extraction has been usually performed using petrochemical and volatile organic solvents, but nowadays, increased recognition of environmental pollution has prompted the utilization of green solvents as alternatives. Therefore, the aim of the present study was to exploit deep eutectic solvents (DES) (choline chloride: citric acid and choline chloride: glucose) as solvents for extracting valuable phenolic antioxidants from grapes. Investigation was conducted on ten grape varieties, observing seeds and skin as different matrix. Total polyphenol content (TPC) was determined by Folin-Ciocalteu spectrophotometric microassay. Antioxidant activity was investigated using four different tests and results were combined in a unique Antioxidant Composite Index (ACI) to reveal comprehensive information about this biological activity. Polyphenol compounds were identified and quantified with the aim of HPLC-diode array detector (DAD). Overall results support that DES (particularly choline chloride: citric acid) were comparable to conventional solvent, and in most cases even outperformed acidified aqueous ethanol (concerning extraction efficiency and antioxidant activity). Regardless of varietal distinctions, grape seeds have higher antioxidant capacity compared to grape skin, and such findings are according to their phenol compound concentrations.
PB  - MDPI AG
T2  - Applied Sciences (Switzerland)
T1  - Impact of deep eutectic solvents on extraction of polyphenols from grape seeds and skin
VL  - 10
IS  - 14
DO  - 10.3390/app10144830
ER  - 

@article{
author = "Dabetić, Nevena and Todorović, Vanja and Panić, Manuela and Radojčić Redovniković, Ivana and Šobajić, Slađana",
year = "2020",
abstract = "In the past few years, research efforts have focused on plant exploitation for deriving some valuable compounds. Extraction has been usually performed using petrochemical and volatile organic solvents, but nowadays, increased recognition of environmental pollution has prompted the utilization of green solvents as alternatives. Therefore, the aim of the present study was to exploit deep eutectic solvents (DES) (choline chloride: citric acid and choline chloride: glucose) as solvents for extracting valuable phenolic antioxidants from grapes. Investigation was conducted on ten grape varieties, observing seeds and skin as different matrix. Total polyphenol content (TPC) was determined by Folin-Ciocalteu spectrophotometric microassay. Antioxidant activity was investigated using four different tests and results were combined in a unique Antioxidant Composite Index (ACI) to reveal comprehensive information about this biological activity. Polyphenol compounds were identified and quantified with the aim of HPLC-diode array detector (DAD). Overall results support that DES (particularly choline chloride: citric acid) were comparable to conventional solvent, and in most cases even outperformed acidified aqueous ethanol (concerning extraction efficiency and antioxidant activity). Regardless of varietal distinctions, grape seeds have higher antioxidant capacity compared to grape skin, and such findings are according to their phenol compound concentrations.",
publisher = "MDPI AG",
journal = "Applied Sciences (Switzerland)",
title = "Impact of deep eutectic solvents on extraction of polyphenols from grape seeds and skin",
volume = "10",
number = "14",
doi = "10.3390/app10144830"
}

Dabetić, N., Todorović, V., Panić, M., Radojčić Redovniković, I.,& Šobajić, S.. (2020). Impact of deep eutectic solvents on extraction of polyphenols from grape seeds and skin. in Applied Sciences (Switzerland)
MDPI AG., 10(14).
https://doi.org/10.3390/app10144830

Dabetić N, Todorović V, Panić M, Radojčić Redovniković I, Šobajić S. Impact of deep eutectic solvents on extraction of polyphenols from grape seeds and skin. in Applied Sciences (Switzerland). 2020;10(14).
doi:10.3390/app10144830 .

Dabetić, Nevena, Todorović, Vanja, Panić, Manuela, Radojčić Redovniković, Ivana, Šobajić, Slađana, "Impact of deep eutectic solvents on extraction of polyphenols from grape seeds and skin" in Applied Sciences (Switzerland), 10, no. 14 (2020),
https://doi.org/10.3390/app10144830 . .

TY  - JOUR
AU  - Todorović, Vanja
AU  - Radojčić-Redovniković, Ivana
AU  - Todorović, Zoran B.
AU  - Janković, Goran
AU  - Dodevska, Margarita
AU  - Šobajić, Slađana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2459
AB  - Different kinds of chocolates produced in Serbia were analyzed regarding total polyphenol, flavonoid and proanthocyanidin content using spectrophotometric methods. Flavan-3ols and methylxanthines in all samples were determined with RP-HPLC. DPPH, FRAP, ABTS and ORAC assays were applied for measuring antioxidant capacity. The average of all four antioxidant tests for each cocoa product was used for calculating antioxidant potency composite index (ACI). Obtained results for all four assays have shown that antioxidant capacity of analyzed chocolate/cocoa extracts followed cocoa, polyphenol, flavonoid, and proanthocyanidin contents. Although the addition of raspberries to dark chocolates had no significant influence on their total polyphenol, flavonoid and proanthocyanidin contents, statistical analysis showed that there was significant increase in the antioxidant capacity of dark chocolates with raspberry compared to plain dark chocolates (p = 0.007). Overall range for theobromine content varied from 5.5 to 22.3 mg/g depending on the product type, while the content of caffeine was 13-30 times lower in all analyzed cocoa products. In addition, correlation between antioxidant potency composite index and declared percentage of cocoa was high (R-2 = 0.798,p  lt  0.05) and indicated that declared cocoa content was a reliable indication for antioxidant capacity of chocolates produced in Serbia.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia
VL  - 41
SP  - 137
EP  - 143
DO  - 10.1016/j.jfca.2015.01.018
ER  - 

@article{
author = "Todorović, Vanja and Radojčić-Redovniković, Ivana and Todorović, Zoran B. and Janković, Goran and Dodevska, Margarita and Šobajić, Slađana",
year = "2015",
abstract = "Different kinds of chocolates produced in Serbia were analyzed regarding total polyphenol, flavonoid and proanthocyanidin content using spectrophotometric methods. Flavan-3ols and methylxanthines in all samples were determined with RP-HPLC. DPPH, FRAP, ABTS and ORAC assays were applied for measuring antioxidant capacity. The average of all four antioxidant tests for each cocoa product was used for calculating antioxidant potency composite index (ACI). Obtained results for all four assays have shown that antioxidant capacity of analyzed chocolate/cocoa extracts followed cocoa, polyphenol, flavonoid, and proanthocyanidin contents. Although the addition of raspberries to dark chocolates had no significant influence on their total polyphenol, flavonoid and proanthocyanidin contents, statistical analysis showed that there was significant increase in the antioxidant capacity of dark chocolates with raspberry compared to plain dark chocolates (p = 0.007). Overall range for theobromine content varied from 5.5 to 22.3 mg/g depending on the product type, while the content of caffeine was 13-30 times lower in all analyzed cocoa products. In addition, correlation between antioxidant potency composite index and declared percentage of cocoa was high (R-2 = 0.798,p  lt  0.05) and indicated that declared cocoa content was a reliable indication for antioxidant capacity of chocolates produced in Serbia.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia",
volume = "41",
pages = "137-143",
doi = "10.1016/j.jfca.2015.01.018"
}

Todorović, V., Radojčić-Redovniković, I., Todorović, Z. B., Janković, G., Dodevska, M.,& Šobajić, S.. (2015). Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 41, 137-143.
https://doi.org/10.1016/j.jfca.2015.01.018

Todorović V, Radojčić-Redovniković I, Todorović ZB, Janković G, Dodevska M, Šobajić S. Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia. in Journal of Food Composition and Analysis. 2015;41:137-143.
doi:10.1016/j.jfca.2015.01.018 .

Todorović, Vanja, Radojčić-Redovniković, Ivana, Todorović, Zoran B., Janković, Goran, Dodevska, Margarita, Šobajić, Slađana, "Polyphenols, methylxanthines, and antioxidant capacity of chocolates produced in Serbia" in Journal of Food Composition and Analysis, 41 (2015):137-143,
https://doi.org/10.1016/j.jfca.2015.01.018 . .