TY  - CONF
AU  - Cvetanović, Aleksandra
AU  - Pavlić, Branimir
AU  - Ražić, Slavica
AU  - Arsenijević, Jelena
AU  - Zengin, Gokhan
AU  - Uysal, Sengul
AU  - Zeković, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5275
AB  - U okviru ovog rada cvast kamilice je ekstrahovana primenom dve različite tehnike, sa ciljem dobijanja proizvoda bogatog bioaktivnim jedinjenjima i funkcionalnim sastojcima. Za ovu svrhu, primenjena je jedna konvencionalna (Soxhlet extrakcija - SE) i jedna nekonvencionalna (superkritična ekstrakcija - SFE) tehnika. Detaljni hemijski sasatav SE i SFE ekstrakata je analiziran primenom GC - MS. Dodatno, izvršena je i procena biološke aktivnosti dobijenih ekstrakata. Prinos lipofine frakcije dobijene nakon tročasovne ekstrakcije čistim ugljen-dioksidom (350 bar i 40 °C) je bio približan prinosu ostvarenom SE ekstrakcijom u trajanju od 6 h pomoću metilen hlorida. Ispitivanje ekstarkata u smislu inhibicije acetilholinesteraze (AChE) i butirilholinesteraze (BChE) je pokazalo veći enzim-inhibitorni potencijala SFE uzorka. Imajući u vidu „zeleni" karakter i bezbednost SFE ekstrakata, prepoznat je veliki potencijal i mogućnost njihove primene kao sastojaka različitih prehrambenih funkcionalnih proizvoda, kao i brojnih farmaceutskih preparata.
AB  - In this study chamomile was extracted applying two different techniques in order to obtain the extract opulent with bioactive compounds. For this purpose one conventional (Soxhlet - SE) and one non-conventional (Supercritical fluid extraction - SFE) extraction technique were used. Detailed chemical composition of SE and SFE extracts was analysed by GC - MS. Additionally, biological activity of the chamomile products was examined as well. The yield of lipophilic fraction, achieved by 3h extraction, with CO2 at 350 bar and 40 °C was close to that one produced by Soxhlet performed for 6 h with methylene chloride. On the other hand, examination of their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) showed much higher enzyme-inhibitory potential of SFE extracts. Due to their green character and safety, SFE extracts have a great potential and possibilities for their use as ingredients in both numerous functional food products and pharmaceutical products, are recognized.
PB  - Univerzitet u Beogradu
C3  - UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova
T1  - Savremen pristup dobijanja funkcionalnih sastojaka kamilice
T1  - Emerging approach for the preparation of chamomile functional ingredients
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5275
ER  - 

@conference{
author = "Cvetanović, Aleksandra and Pavlić, Branimir and Ražić, Slavica and Arsenijević, Jelena and Zengin, Gokhan and Uysal, Sengul and Zeković, Zoran",
year = "2018",
abstract = "U okviru ovog rada cvast kamilice je ekstrahovana primenom dve različite tehnike, sa ciljem dobijanja proizvoda bogatog bioaktivnim jedinjenjima i funkcionalnim sastojcima. Za ovu svrhu, primenjena je jedna konvencionalna (Soxhlet extrakcija - SE) i jedna nekonvencionalna (superkritična ekstrakcija - SFE) tehnika. Detaljni hemijski sasatav SE i SFE ekstrakata je analiziran primenom GC - MS. Dodatno, izvršena je i procena biološke aktivnosti dobijenih ekstrakata. Prinos lipofine frakcije dobijene nakon tročasovne ekstrakcije čistim ugljen-dioksidom (350 bar i 40 °C) je bio približan prinosu ostvarenom SE ekstrakcijom u trajanju od 6 h pomoću metilen hlorida. Ispitivanje ekstarkata u smislu inhibicije acetilholinesteraze (AChE) i butirilholinesteraze (BChE) je pokazalo veći enzim-inhibitorni potencijala SFE uzorka. Imajući u vidu „zeleni" karakter i bezbednost SFE ekstrakata, prepoznat je veliki potencijal i mogućnost njihove primene kao sastojaka različitih prehrambenih funkcionalnih proizvoda, kao i brojnih farmaceutskih preparata., In this study chamomile was extracted applying two different techniques in order to obtain the extract opulent with bioactive compounds. For this purpose one conventional (Soxhlet - SE) and one non-conventional (Supercritical fluid extraction - SFE) extraction technique were used. Detailed chemical composition of SE and SFE extracts was analysed by GC - MS. Additionally, biological activity of the chamomile products was examined as well. The yield of lipophilic fraction, achieved by 3h extraction, with CO2 at 350 bar and 40 °C was close to that one produced by Soxhlet performed for 6 h with methylene chloride. On the other hand, examination of their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) showed much higher enzyme-inhibitory potential of SFE extracts. Due to their green character and safety, SFE extracts have a great potential and possibilities for their use as ingredients in both numerous functional food products and pharmaceutical products, are recognized.",
publisher = "Univerzitet u Beogradu",
journal = "UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova",
title = "Savremen pristup dobijanja funkcionalnih sastojaka kamilice, Emerging approach for the preparation of chamomile functional ingredients",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5275"
}

Cvetanović, A., Pavlić, B., Ražić, S., Arsenijević, J., Zengin, G., Uysal, S.,& Zeković, Z.. (2018). Savremen pristup dobijanja funkcionalnih sastojaka kamilice. in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova
Univerzitet u Beogradu..
https://hdl.handle.net/21.15107/rcub_farfar_5275

Cvetanović A, Pavlić B, Ražić S, Arsenijević J, Zengin G, Uysal S, Zeković Z. Savremen pristup dobijanja funkcionalnih sastojaka kamilice. in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova. 2018;.
https://hdl.handle.net/21.15107/rcub_farfar_5275 .

Cvetanović, Aleksandra, Pavlić, Branimir, Ražić, Slavica, Arsenijević, Jelena, Zengin, Gokhan, Uysal, Sengul, Zeković, Zoran, "Savremen pristup dobijanja funkcionalnih sastojaka kamilice" in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova (2018),
https://hdl.handle.net/21.15107/rcub_farfar_5275 .

TY  - JOUR
AU  - Cvetanović, Aleksandra
AU  - Zekovic, Zoran
AU  - Svarc-Gajic, Jaroslava
AU  - Ražić, Slavica
AU  - Damjanović, Ana B.
AU  - Zengin, Gokhan
AU  - Delerue-Matos, Cristina
AU  - Moreira, Manuela
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3194
AB  - BACKGROUND: To obtain bioactive fractions from plant material subcritical water is notably advantageous in comparison to any other technique. Excellent solvating properties and selectivity combined with finely tuned reactivity of subcritical water enable exploitation of the potential of plants. Herein subcritical water extraction was used for recovery of bioactive compounds from leaves, roots and fruits of Sambucus ebulus L. Extracts obtained were characterized in terms of biological and chemical fingerprints. RESULTS AND DISCUSSIONR: esults obtained by using several antioxidant assays that focused on different mechanisms showed that subcritical water extracts of Sambucus ebulus were powerful antioxidants. Enzyme inhibitory effects were tested against alpha-amylase, alpha-glucosidase and tyrosinase, and the results showed the anti-diabetic potential of the extracts as well as its possible use with skin disorders. Antiproliferative properties were detected on three cell lines (A-549; LS-174 T; HeLa) and showed prominent cytotoxicity against all tumor cell lines. Inhibitory concentrations obtained were in the range 0.58-8.10 mu g mL(-1). Generally, the SCW extracts from leaves exhibited stronger biological activities with higher levels of phenolic compounds compared with the roots and fruits of Sambucus ebulus. Gallic acid, catechin and caffeic acid were identified as major components in these extracts and these components seem to relate with observed biological activity. CONCLUSION: The results obtained suggest that Sambucus ebulus has great potential for preparing new phyto-pharmaceuticals and functional food ingredients. (C) 2017 Society of Chemical Industry.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.
VL  - 93
IS  - 4
SP  - 1097
EP  - 1104
DO  - 10.1002/jctb.5468
ER  - 

@article{
author = "Cvetanović, Aleksandra and Zekovic, Zoran and Svarc-Gajic, Jaroslava and Ražić, Slavica and Damjanović, Ana B. and Zengin, Gokhan and Delerue-Matos, Cristina and Moreira, Manuela",
year = "2018",
abstract = "BACKGROUND: To obtain bioactive fractions from plant material subcritical water is notably advantageous in comparison to any other technique. Excellent solvating properties and selectivity combined with finely tuned reactivity of subcritical water enable exploitation of the potential of plants. Herein subcritical water extraction was used for recovery of bioactive compounds from leaves, roots and fruits of Sambucus ebulus L. Extracts obtained were characterized in terms of biological and chemical fingerprints. RESULTS AND DISCUSSIONR: esults obtained by using several antioxidant assays that focused on different mechanisms showed that subcritical water extracts of Sambucus ebulus were powerful antioxidants. Enzyme inhibitory effects were tested against alpha-amylase, alpha-glucosidase and tyrosinase, and the results showed the anti-diabetic potential of the extracts as well as its possible use with skin disorders. Antiproliferative properties were detected on three cell lines (A-549; LS-174 T; HeLa) and showed prominent cytotoxicity against all tumor cell lines. Inhibitory concentrations obtained were in the range 0.58-8.10 mu g mL(-1). Generally, the SCW extracts from leaves exhibited stronger biological activities with higher levels of phenolic compounds compared with the roots and fruits of Sambucus ebulus. Gallic acid, catechin and caffeic acid were identified as major components in these extracts and these components seem to relate with observed biological activity. CONCLUSION: The results obtained suggest that Sambucus ebulus has great potential for preparing new phyto-pharmaceuticals and functional food ingredients. (C) 2017 Society of Chemical Industry.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.",
volume = "93",
number = "4",
pages = "1097-1104",
doi = "10.1002/jctb.5468"
}

Cvetanović, A., Zekovic, Z., Svarc-Gajic, J., Ražić, S., Damjanović, A. B., Zengin, G., Delerue-Matos, C.,& Moreira, M.. (2018). A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 93(4), 1097-1104.
https://doi.org/10.1002/jctb.5468

Cvetanović A, Zekovic Z, Svarc-Gajic J, Ražić S, Damjanović AB, Zengin G, Delerue-Matos C, Moreira M. A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L.. in Journal of Chemical Technology and Biotechnology. 2018;93(4):1097-1104.
doi:10.1002/jctb.5468 .

Cvetanović, Aleksandra, Zekovic, Zoran, Svarc-Gajic, Jaroslava, Ražić, Slavica, Damjanović, Ana B., Zengin, Gokhan, Delerue-Matos, Cristina, Moreira, Manuela, "A new source for developing multi-functional products: biological and chemical perspectives on subcritical water extracts of Sambucus ebulus L." in Journal of Chemical Technology and Biotechnology, 93, no. 4 (2018):1097-1104,
https://doi.org/10.1002/jctb.5468 . .

TY  - JOUR
AU  - Cvetanović, Aleksandra
AU  - Zengin, Gokhan
AU  - Zekovic, Zoran
AU  - Svarc-Gajic, Jaroslava
AU  - Ražić, Slavica
AU  - Damjanović, Ana B.
AU  - Magkovic, Pavle
AU  - Mitic, Milan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3056
AB  - Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction
VL  - 121
SP  - 458
EP  - 466
DO  - 10.1016/j.fct.2018.09.045
ER  - 

@article{
author = "Cvetanović, Aleksandra and Zengin, Gokhan and Zekovic, Zoran and Svarc-Gajic, Jaroslava and Ražić, Slavica and Damjanović, Ana B. and Magkovic, Pavle and Mitic, Milan",
year = "2018",
abstract = "Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction",
volume = "121",
pages = "458-466",
doi = "10.1016/j.fct.2018.09.045"
}

Cvetanović, A., Zengin, G., Zekovic, Z., Svarc-Gajic, J., Ražić, S., Damjanović, A. B., Magkovic, P.,& Mitic, M.. (2018). Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 121, 458-466.
https://doi.org/10.1016/j.fct.2018.09.045

Cvetanović A, Zengin G, Zekovic Z, Svarc-Gajic J, Ražić S, Damjanović AB, Magkovic P, Mitic M. Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology. 2018;121:458-466.
doi:10.1016/j.fct.2018.09.045 .

Cvetanović, Aleksandra, Zengin, Gokhan, Zekovic, Zoran, Svarc-Gajic, Jaroslava, Ražić, Slavica, Damjanović, Ana B., Magkovic, Pavle, Mitic, Milan, "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction" in Food and Chemical Toxicology, 121 (2018):458-466,
https://doi.org/10.1016/j.fct.2018.09.045 . .