TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Stajić, Mirjana
AU  - Živković, Lada
AU  - Milovanović, Ivan
AU  - Potparević, Biljana
AU  - Vukojević, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2926
AB  - This study aimed to evaluate the antifungal, antioxidative, and genoprotective potentials of various extracts of Daedaleopsis confragosa fruiting bodies and mycelia. The extracts exhibited weak fungistatic and an absence of any fungicidal activities against 7 important animal and human pathogens. Although mycelial extracts, especially the hot water extract, were more effective antifungal agents than basidiocarp extracts, they were significantly weaker than the commercial antimycotic ketoconazole. The tested extracts reduced DPPH radicals to various extents, depending on the material used for extraction and the solvent. The mycelial hot water extract was the best DPPH radical scavenger (half-maximal effective concentration, 3.42 mg mL-1), whereas the 96% ethanol extract of basidiocarps was the weakest one (half-maximal effective concentration, 5.36 mg mL-1). Phenols were the main carriers of activity, and their proportions in the tested extract mainly depended on the type and concentration of the solvent; the effect of the material used for extraction was negligible. The largest amount of phenols was detected in the 70% ethanol extract of basidiocarps (126.40 mu g gallic acid equivalents mg-1) and the lowest in the basidiocarp methanol extract (19.93 mu g gallic acid equivalents mg-1). Aqueous extracts of basidiocarp and mycelial extracts were not genotoxic agents but exhibited significant protective activity against hydrogen peroxide-induced DNA damage during use in leukocytes' pre-and post-treatments.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Antifungal, Antioxidative, and Genoprotective Properties of Extracts from the Blushing Bracket Mushroom, Daedaleopsis confragosa (Agaricomycetes)
VL  - 19
IS  - 6
SP  - 509
EP  - 520
DO  - 10.1615/IntJMedMushrooms.v19.i6.30
ER  - 

@article{
author = "Knežević, Aleksandar and Stajić, Mirjana and Živković, Lada and Milovanović, Ivan and Potparević, Biljana and Vukojević, Jelena",
year = "2017",
abstract = "This study aimed to evaluate the antifungal, antioxidative, and genoprotective potentials of various extracts of Daedaleopsis confragosa fruiting bodies and mycelia. The extracts exhibited weak fungistatic and an absence of any fungicidal activities against 7 important animal and human pathogens. Although mycelial extracts, especially the hot water extract, were more effective antifungal agents than basidiocarp extracts, they were significantly weaker than the commercial antimycotic ketoconazole. The tested extracts reduced DPPH radicals to various extents, depending on the material used for extraction and the solvent. The mycelial hot water extract was the best DPPH radical scavenger (half-maximal effective concentration, 3.42 mg mL-1), whereas the 96% ethanol extract of basidiocarps was the weakest one (half-maximal effective concentration, 5.36 mg mL-1). Phenols were the main carriers of activity, and their proportions in the tested extract mainly depended on the type and concentration of the solvent; the effect of the material used for extraction was negligible. The largest amount of phenols was detected in the 70% ethanol extract of basidiocarps (126.40 mu g gallic acid equivalents mg-1) and the lowest in the basidiocarp methanol extract (19.93 mu g gallic acid equivalents mg-1). Aqueous extracts of basidiocarp and mycelial extracts were not genotoxic agents but exhibited significant protective activity against hydrogen peroxide-induced DNA damage during use in leukocytes' pre-and post-treatments.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Antifungal, Antioxidative, and Genoprotective Properties of Extracts from the Blushing Bracket Mushroom, Daedaleopsis confragosa (Agaricomycetes)",
volume = "19",
number = "6",
pages = "509-520",
doi = "10.1615/IntJMedMushrooms.v19.i6.30"
}

Knežević, A., Stajić, M., Živković, L., Milovanović, I., Potparević, B.,& Vukojević, J.. (2017). Antifungal, Antioxidative, and Genoprotective Properties of Extracts from the Blushing Bracket Mushroom, Daedaleopsis confragosa (Agaricomycetes). in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 19(6), 509-520.
https://doi.org/10.1615/IntJMedMushrooms.v19.i6.30

Knežević A, Stajić M, Živković L, Milovanović I, Potparević B, Vukojević J. Antifungal, Antioxidative, and Genoprotective Properties of Extracts from the Blushing Bracket Mushroom, Daedaleopsis confragosa (Agaricomycetes). in International Journal of Medicinal Mushrooms. 2017;19(6):509-520.
doi:10.1615/IntJMedMushrooms.v19.i6.30 .

Knežević, Aleksandar, Stajić, Mirjana, Živković, Lada, Milovanović, Ivan, Potparević, Biljana, Vukojević, Jelena, "Antifungal, Antioxidative, and Genoprotective Properties of Extracts from the Blushing Bracket Mushroom, Daedaleopsis confragosa (Agaricomycetes)" in International Journal of Medicinal Mushrooms, 19, no. 6 (2017):509-520,
https://doi.org/10.1615/IntJMedMushrooms.v19.i6.30 . .

TY  - JOUR
AU  - Cilerdzić, Jasmina
AU  - Stajić, Mirjana
AU  - Živković, Lada
AU  - Vukojević, Jelena
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2680
AB  - Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps
VL  - 18
IS  - 12
SP  - 1061
EP  - 1069
DO  - 10.1615/IntJMedMushrooms.v18.i12.10
ER  - 

@article{
author = "Cilerdzić, Jasmina and Stajić, Mirjana and Živković, Lada and Vukojević, Jelena and Bajić, Vladan and Potparević, Biljana",
year = "2016",
abstract = "Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps",
volume = "18",
number = "12",
pages = "1061-1069",
doi = "10.1615/IntJMedMushrooms.v18.i12.10"
}

Cilerdzić, J., Stajić, M., Živković, L., Vukojević, J., Bajić, V.,& Potparević, B.. (2016). Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 18(12), 1061-1069.
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10

Cilerdzić J, Stajić M, Živković L, Vukojević J, Bajić V, Potparević B. Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps. in International Journal of Medicinal Mushrooms. 2016;18(12):1061-1069.
doi:10.1615/IntJMedMushrooms.v18.i12.10 .

Cilerdzić, Jasmina, Stajić, Mirjana, Živković, Lada, Vukojević, Jelena, Bajić, Vladan, Potparević, Biljana, "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps" in International Journal of Medicinal Mushrooms, 18, no. 12 (2016):1061-1069,
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10 . .

TY  - JOUR
AU  - Knežević, A
AU  - Živković, Lada
AU  - Stajić, Mirjana
AU  - Vukojević, Jelena
AU  - Milovanović, I
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2495
AB  - Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.
PB  - Hindawi Publishing Corporation
T2  - Scientific World Journal
T1  - Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells
VL  - 2015
DO  - 10.1155/2015/146378
ER  - 

@article{
author = "Knežević, A and Živković, Lada and Stajić, Mirjana and Vukojević, Jelena and Milovanović, I and Potparević, Biljana",
year = "2015",
abstract = "Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.",
publisher = "Hindawi Publishing Corporation",
journal = "Scientific World Journal",
title = "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells",
volume = "2015",
doi = "10.1155/2015/146378"
}

Knežević, A., Živković, L., Stajić, M., Vukojević, J., Milovanović, I.,& Potparević, B.. (2015). Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal
Hindawi Publishing Corporation., 2015.
https://doi.org/10.1155/2015/146378

Knežević A, Živković L, Stajić M, Vukojević J, Milovanović I, Potparević B. Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal. 2015;2015.
doi:10.1155/2015/146378 .

Knežević, A, Živković, Lada, Stajić, Mirjana, Vukojević, Jelena, Milovanović, I, Potparević, Biljana, "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells" in Scientific World Journal, 2015 (2015),
https://doi.org/10.1155/2015/146378 . .