TY  - JOUR
AU  - Gopčević, Kristina
AU  - Grujić, Slavica
AU  - Arsenijević, Jelena
AU  - Džamić, Ana
AU  - Veličković, Ivona
AU  - Izrael-Živković, Lidija
AU  - Medić, Ana
AU  - Mudrić, Jelena
AU  - Soković, Marina
AU  - Đurić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4342
AB  - The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.
PB  - Nature Research
T2  - Scientific Reports
T1  - Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction
VL  - 12
IS  - 1
DO  - 10.1038/s41598-022-25668-3
ER  - 

@article{
author = "Gopčević, Kristina and Grujić, Slavica and Arsenijević, Jelena and Džamić, Ana and Veličković, Ivona and Izrael-Živković, Lidija and Medić, Ana and Mudrić, Jelena and Soković, Marina and Đurić, Ana",
year = "2022",
abstract = "The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction",
volume = "12",
number = "1",
doi = "10.1038/s41598-022-25668-3"
}

Gopčević, K., Grujić, S., Arsenijević, J., Džamić, A., Veličković, I., Izrael-Živković, L., Medić, A., Mudrić, J., Soković, M.,& Đurić, A.. (2022). Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports
Nature Research., 12(1).
https://doi.org/10.1038/s41598-022-25668-3

Gopčević K, Grujić S, Arsenijević J, Džamić A, Veličković I, Izrael-Živković L, Medić A, Mudrić J, Soković M, Đurić A. Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports. 2022;12(1).
doi:10.1038/s41598-022-25668-3 .

Gopčević, Kristina, Grujić, Slavica, Arsenijević, Jelena, Džamić, Ana, Veličković, Ivona, Izrael-Živković, Lidija, Medić, Ana, Mudrić, Jelena, Soković, Marina, Đurić, Ana, "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction" in Scientific Reports, 12, no. 1 (2022),
https://doi.org/10.1038/s41598-022-25668-3 . .

TY  - JOUR
AU  - Mudrić, Jelena
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
AU  - Ibrić, Svetlana
AU  - Gopčević, Kristina
AU  - Đuriš, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3953
AB  - Aerial parts of Satureja kitaibelii are traditionally used as carminative, for treatment of respiratory and urinary  complaints, or as anti-inflammatory agent for skin and mucous membranes. The aim of the present study was to  formulate immediate-release capsules and tablets with high content (≥95%) of S. kitaibelii optimized dry extract  by direct compression. The influence of ethanol concentration (40–96%, v/v), temperature (25–80 ◦C) and time  (2–8 h) on the extraction yield, total phenolic content (TPC), as well as on the contents of rosmarinic acid (RA)  and clinopodic acid O (CAO) in the S. kitaibelii dry extract was investigated using the response surface meth-odology (RSM). The estimated optimal conditions for the extraction were: ethanol 46.4%, 61.6 ◦C, 2 h. Dry  extract of S. kitaibelii obtained under the optimal conditions (characterized by: yield 24.2%, TPC 170.4 mg gallic  acid equivalents/g, RA 10.7 mg/g and CAO 17.1 mg/g) was used to manufacture solid dosage forms. Three  formulations (F) were prepared: F1, hard capsules (99% dry extract, 1% magnesium stearate); F2, uncoated  tablets (99% dry extract, 1% magnesium stearate) and F3, uncoated tablets (95% dry extract, 4% sodium starch  glycolate, 1% magnesium stearate). Hard capsules (F1) with the uniform weight, adequate disintegration time  and dissolution rate were obtained. Regarding tablet formulations, F3 exhibited satisfactory tableting properties,  disintegration time and dissolution profile. Therefore, immediate-release capsules and tablets with a high content  of S.kitaibelii extract were formulated to accomplish the unmet need for convenient solid dosage forms that  contains high doses of herbal extracts.
PB  - Elsevier B.V.
T2  - Journal of Drug Delivery Science and Technology
T1  - Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii
VL  - 66
DO  - 10.1016/j.jddst.2021.102776
ER  - 

@article{
author = "Mudrić, Jelena and Arsenijević, Jelena and Maksimović, Zoran and Ibrić, Svetlana and Gopčević, Kristina and Đuriš, Jelena",
year = "2021",
abstract = "Aerial parts of Satureja kitaibelii are traditionally used as carminative, for treatment of respiratory and urinary  complaints, or as anti-inflammatory agent for skin and mucous membranes. The aim of the present study was to  formulate immediate-release capsules and tablets with high content (≥95%) of S. kitaibelii optimized dry extract  by direct compression. The influence of ethanol concentration (40–96%, v/v), temperature (25–80 ◦C) and time  (2–8 h) on the extraction yield, total phenolic content (TPC), as well as on the contents of rosmarinic acid (RA)  and clinopodic acid O (CAO) in the S. kitaibelii dry extract was investigated using the response surface meth-odology (RSM). The estimated optimal conditions for the extraction were: ethanol 46.4%, 61.6 ◦C, 2 h. Dry  extract of S. kitaibelii obtained under the optimal conditions (characterized by: yield 24.2%, TPC 170.4 mg gallic  acid equivalents/g, RA 10.7 mg/g and CAO 17.1 mg/g) was used to manufacture solid dosage forms. Three  formulations (F) were prepared: F1, hard capsules (99% dry extract, 1% magnesium stearate); F2, uncoated  tablets (99% dry extract, 1% magnesium stearate) and F3, uncoated tablets (95% dry extract, 4% sodium starch  glycolate, 1% magnesium stearate). Hard capsules (F1) with the uniform weight, adequate disintegration time  and dissolution rate were obtained. Regarding tablet formulations, F3 exhibited satisfactory tableting properties,  disintegration time and dissolution profile. Therefore, immediate-release capsules and tablets with a high content  of S.kitaibelii extract were formulated to accomplish the unmet need for convenient solid dosage forms that  contains high doses of herbal extracts.",
publisher = "Elsevier B.V.",
journal = "Journal of Drug Delivery Science and Technology",
title = "Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii",
volume = "66",
doi = "10.1016/j.jddst.2021.102776"
}

Mudrić, J., Arsenijević, J., Maksimović, Z., Ibrić, S., Gopčević, K.,& Đuriš, J.. (2021). Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii. in Journal of Drug Delivery Science and Technology
Elsevier B.V.., 66.
https://doi.org/10.1016/j.jddst.2021.102776

Mudrić J, Arsenijević J, Maksimović Z, Ibrić S, Gopčević K, Đuriš J. Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii. in Journal of Drug Delivery Science and Technology. 2021;66.
doi:10.1016/j.jddst.2021.102776 .

Mudrić, Jelena, Arsenijević, Jelena, Maksimović, Zoran, Ibrić, Svetlana, Gopčević, Kristina, Đuriš, Jelena, "Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii" in Journal of Drug Delivery Science and Technology, 66 (2021),
https://doi.org/10.1016/j.jddst.2021.102776 . .