TY  - JOUR
AU  - Ivanović, Stefan
AU  - Gođevac, Dejan
AU  - Ristivojević, Petar
AU  - Zdunić, Gordana
AU  - Stojanović, Danilo
AU  - Šavikin, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4366
AB  - Introduction: Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and medicinal use. The aims of this study are: i) to investigate of HPTLC profile of the berry samples using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of investigated chokeberry adulterations iii) to identify the most important botanical markers responsible for classification. Methods: A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in combination with multivariate techniques for differentiating chokeberry samples and identification of the main metabolites of chokeberry and four common adulterants. Images of HPTLC chromatograms obtained using two different detections i.e. visible light and derivatization with natural products reagent were used as a dataset for multivariate analysis. Results: Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of five botanically different samples and recognized their main markers such as responsible for differences between chokeberry and four common adulterants. Conclusion: The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration.
PB  - Elsevier GmbH
T2  - Journal of Herbal Medicine
T1  - HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration
VL  - 37
DO  - 10.1016/j.hermed.2022.100618
ER  - 

@article{
author = "Ivanović, Stefan and Gođevac, Dejan and Ristivojević, Petar and Zdunić, Gordana and Stojanović, Danilo and Šavikin, Katarina",
year = "2023",
abstract = "Introduction: Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and medicinal use. The aims of this study are: i) to investigate of HPTLC profile of the berry samples using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of investigated chokeberry adulterations iii) to identify the most important botanical markers responsible for classification. Methods: A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in combination with multivariate techniques for differentiating chokeberry samples and identification of the main metabolites of chokeberry and four common adulterants. Images of HPTLC chromatograms obtained using two different detections i.e. visible light and derivatization with natural products reagent were used as a dataset for multivariate analysis. Results: Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of five botanically different samples and recognized their main markers such as responsible for differences between chokeberry and four common adulterants. Conclusion: The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration.",
publisher = "Elsevier GmbH",
journal = "Journal of Herbal Medicine",
title = "HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration",
volume = "37",
doi = "10.1016/j.hermed.2022.100618"
}

Ivanović, S., Gođevac, D., Ristivojević, P., Zdunić, G., Stojanović, D.,& Šavikin, K.. (2023). HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration. in Journal of Herbal Medicine
Elsevier GmbH., 37.
https://doi.org/10.1016/j.hermed.2022.100618

Ivanović S, Gođevac D, Ristivojević P, Zdunić G, Stojanović D, Šavikin K. HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration. in Journal of Herbal Medicine. 2023;37.
doi:10.1016/j.hermed.2022.100618 .

Ivanović, Stefan, Gođevac, Dejan, Ristivojević, Petar, Zdunić, Gordana, Stojanović, Danilo, Šavikin, Katarina, "HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration" in Journal of Herbal Medicine, 37 (2023),
https://doi.org/10.1016/j.hermed.2022.100618 . .

TY  - JOUR
AU  - Mudrić, Jelena
AU  - Šavikin, Katarina
AU  - Ðekić, Ljiljana
AU  - Pavlović, Stefan
AU  - Kurćubić, Ivana
AU  - Ibrić, Svetlana
AU  - Ðuriš, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4014
AB  - Gentian (Gentiana lutea L., Gentianaceae) root extract (GRE) is used for the treatment of gastrointestinal disorders. However, its bioactive potential is limited in conventional forms due to the low bioavailability and short elimination half-life of the dominant bioactive compound, gentiopicroside. The aim of study was to encapsulate GRE in the lipid-based gastroretentive delivery system that could provide high yield and encapsulation efficiency, as well as the biphasic release of gentiopicroside from the tablets obtained by direct compression. Solid lipid microparticles (SLM) loaded with GRE were prepared by freeze-drying double (W/O/W) emulsions, which were obtained by a multiple emulsion–melt dispersion technique, with GRE as the inner water phase, Gelucire® 39/01 or 43/01, as lipid components, with or without the addition of porous silica (Sylysia® 350) in the outer water phase. Formulated SLM powders were examined by SEM and mercury intrusion porosimetry, as well as by determination of yield, encapsulation efficiency, and flow properties. Furthermore, in vitro dissolution of gentiopicroside, the size of the dispersed systems, mechanical properties, and mucoadhesion of tablets obtained by direct compression were investigated. The results have revealed that SLM with the macroporous structure were formulated, and, consequently, the powders floated immediately in the acidic medium. Formulation with porous silica (Sylysia® 350) and Gelucire® 43/01 as a solid lipid was characterized with the high yield end encapsulation efficiency. Furthermore, the mucoadhesive properties of tablets obtained by direct compression of that formulation, as well as the biphasic release of gentiopicroside, presence of nanoassociates in dissolution medium, and optimal mechanical properties indicated that a promising lipid-based gastroretentive system for GRE was developed.
PB  - MDPI
T2  - Pharmaceutics
T1  - Development of lipid-based gastroretentive delivery system for gentian extract by double emulsion–melt dispersion technique
VL  - 13
IS  - 12
DO  - 10.3390/pharmaceutics13122095
ER  - 

@article{
author = "Mudrić, Jelena and Šavikin, Katarina and Ðekić, Ljiljana and Pavlović, Stefan and Kurćubić, Ivana and Ibrić, Svetlana and Ðuriš, Jelena",
year = "2021",
abstract = "Gentian (Gentiana lutea L., Gentianaceae) root extract (GRE) is used for the treatment of gastrointestinal disorders. However, its bioactive potential is limited in conventional forms due to the low bioavailability and short elimination half-life of the dominant bioactive compound, gentiopicroside. The aim of study was to encapsulate GRE in the lipid-based gastroretentive delivery system that could provide high yield and encapsulation efficiency, as well as the biphasic release of gentiopicroside from the tablets obtained by direct compression. Solid lipid microparticles (SLM) loaded with GRE were prepared by freeze-drying double (W/O/W) emulsions, which were obtained by a multiple emulsion–melt dispersion technique, with GRE as the inner water phase, Gelucire® 39/01 or 43/01, as lipid components, with or without the addition of porous silica (Sylysia® 350) in the outer water phase. Formulated SLM powders were examined by SEM and mercury intrusion porosimetry, as well as by determination of yield, encapsulation efficiency, and flow properties. Furthermore, in vitro dissolution of gentiopicroside, the size of the dispersed systems, mechanical properties, and mucoadhesion of tablets obtained by direct compression were investigated. The results have revealed that SLM with the macroporous structure were formulated, and, consequently, the powders floated immediately in the acidic medium. Formulation with porous silica (Sylysia® 350) and Gelucire® 43/01 as a solid lipid was characterized with the high yield end encapsulation efficiency. Furthermore, the mucoadhesive properties of tablets obtained by direct compression of that formulation, as well as the biphasic release of gentiopicroside, presence of nanoassociates in dissolution medium, and optimal mechanical properties indicated that a promising lipid-based gastroretentive system for GRE was developed.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Development of lipid-based gastroretentive delivery system for gentian extract by double emulsion–melt dispersion technique",
volume = "13",
number = "12",
doi = "10.3390/pharmaceutics13122095"
}

Mudrić, J., Šavikin, K., Ðekić, L., Pavlović, S., Kurćubić, I., Ibrić, S.,& Ðuriš, J.. (2021). Development of lipid-based gastroretentive delivery system for gentian extract by double emulsion–melt dispersion technique. in Pharmaceutics
MDPI., 13(12).
https://doi.org/10.3390/pharmaceutics13122095

Mudrić J, Šavikin K, Ðekić L, Pavlović S, Kurćubić I, Ibrić S, Ðuriš J. Development of lipid-based gastroretentive delivery system for gentian extract by double emulsion–melt dispersion technique. in Pharmaceutics. 2021;13(12).
doi:10.3390/pharmaceutics13122095 .

Mudrić, Jelena, Šavikin, Katarina, Ðekić, Ljiljana, Pavlović, Stefan, Kurćubić, Ivana, Ibrić, Svetlana, Ðuriš, Jelena, "Development of lipid-based gastroretentive delivery system for gentian extract by double emulsion–melt dispersion technique" in Pharmaceutics, 13, no. 12 (2021),
https://doi.org/10.3390/pharmaceutics13122095 . .

TY  - JOUR
AU  - Damjanović, Ana
AU  - Kolundžija, Branka
AU  - Matić, Ivana
AU  - Krivokuća, Ana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Janković, Radmila
AU  - Antić-Stanković, Jelena
AU  - Stanojković, Tatjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3738
AB  - Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.
PB  - MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro
VL  - 25
IS  - 22
DO  - 10.3390/molecules25225233
ER  - 

@article{
author = "Damjanović, Ana and Kolundžija, Branka and Matić, Ivana and Krivokuća, Ana and Zdunić, Gordana and Šavikin, Katarina and Janković, Radmila and Antić-Stanković, Jelena and Stanojković, Tatjana",
year = "2020",
abstract = "Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.",
publisher = "MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro",
volume = "25",
number = "22",
doi = "10.3390/molecules25225233"
}

Damjanović, A., Kolundžija, B., Matić, I., Krivokuća, A., Zdunić, G., Šavikin, K., Janković, R., Antić-Stanković, J.,& Stanojković, T.. (2020). Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland)
MDPI., 25(22).
https://doi.org/10.3390/molecules25225233

Damjanović A, Kolundžija B, Matić I, Krivokuća A, Zdunić G, Šavikin K, Janković R, Antić-Stanković J, Stanojković T. Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. in Molecules (Basel, Switzerland). 2020;25(22).
doi:10.3390/molecules25225233 .

Damjanović, Ana, Kolundžija, Branka, Matić, Ivana, Krivokuća, Ana, Zdunić, Gordana, Šavikin, Katarina, Janković, Radmila, Antić-Stanković, Jelena, Stanojković, Tatjana, "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro" in Molecules (Basel, Switzerland), 25, no. 22 (2020),
https://doi.org/10.3390/molecules25225233 . .

TY  - JOUR
AU  - Mudrić, Jelena
AU  - Janković, Teodora
AU  - Šavikin, Katarina
AU  - Bigović, Dubravka
AU  - Đukić-Ćosić, Danijela
AU  - Ibrić, Svetlana
AU  - Đuriš, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3636
AB  - Perennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots
VL  - 155
DO  - 10.1016/j.indcrop.2020.112767
ER  - 

@article{
author = "Mudrić, Jelena and Janković, Teodora and Šavikin, Katarina and Bigović, Dubravka and Đukić-Ćosić, Danijela and Ibrić, Svetlana and Đuriš, Jelena",
year = "2020",
abstract = "Perennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots",
volume = "155",
doi = "10.1016/j.indcrop.2020.112767"
}

Mudrić, J., Janković, T., Šavikin, K., Bigović, D., Đukić-Ćosić, D., Ibrić, S.,& Đuriš, J.. (2020). Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots. in Industrial Crops and Products
Elsevier B.V.., 155.
https://doi.org/10.1016/j.indcrop.2020.112767

Mudrić J, Janković T, Šavikin K, Bigović D, Đukić-Ćosić D, Ibrić S, Đuriš J. Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots. in Industrial Crops and Products. 2020;155.
doi:10.1016/j.indcrop.2020.112767 .

Mudrić, Jelena, Janković, Teodora, Šavikin, Katarina, Bigović, Dubravka, Đukić-Ćosić, Danijela, Ibrić, Svetlana, Đuriš, Jelena, "Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots" in Industrial Crops and Products, 155 (2020),
https://doi.org/10.1016/j.indcrop.2020.112767 . .

TY  - JOUR
AU  - Grozdanić, Nađa
AU  - Đuričić, Ivana
AU  - Kosanić, Marijana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Etahiri, Samira
AU  - Assobhei, Omar
AU  - Benba, Jamila
AU  - Petović, Slavica
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3605
AB  - Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
PB  - Zerbinis Publications
T2  - Journal of B.U.ON.
T1  - Fucus spiralis extract and fractions: Anticancer and pharmacological potentials
VL  - 25
IS  - 2
SP  - 1219
EP  - 1229
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3605
ER  - 

@article{
author = "Grozdanić, Nađa and Đuričić, Ivana and Kosanić, Marijana and Zdunić, Gordana and Šavikin, Katarina and Etahiri, Samira and Assobhei, Omar and Benba, Jamila and Petović, Slavica and Matić, Ivana Z. and Stanojković, Tatjana P.",
year = "2020",
abstract = "Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.",
publisher = "Zerbinis Publications",
journal = "Journal of B.U.ON.",
title = "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials",
volume = "25",
number = "2",
pages = "1219-1229",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3605"
}

Grozdanić, N., Đuričić, I., Kosanić, M., Zdunić, G., Šavikin, K., Etahiri, S., Assobhei, O., Benba, J., Petović, S., Matić, I. Z.,& Stanojković, T. P.. (2020). Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.
Zerbinis Publications., 25(2), 1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605

Grozdanić N, Đuričić I, Kosanić M, Zdunić G, Šavikin K, Etahiri S, Assobhei O, Benba J, Petović S, Matić IZ, Stanojković TP. Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.. 2020;25(2):1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

Grozdanić, Nađa, Đuričić, Ivana, Kosanić, Marijana, Zdunić, Gordana, Šavikin, Katarina, Etahiri, Samira, Assobhei, Omar, Benba, Jamila, Petović, Slavica, Matić, Ivana Z., Stanojković, Tatjana P., "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials" in Journal of B.U.ON., 25, no. 2 (2020):1219-1229,
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Šavikin, Katarina
AU  - Petrović, Silvana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2859
AB  - A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H hirsutum, H maculatum, and H richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3,II8-biapigenin, orientin, 2"-O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H acutum and H maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties
VL  - 12
IS  - 11
SP  - 1805
EP  - 1811
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2859
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Šavikin, Katarina and Petrović, Silvana",
year = "2017",
abstract = "A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H hirsutum, H maculatum, and H richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3,II8-biapigenin, orientin, 2"-O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3-O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H acutum and H maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties",
volume = "12",
number = "11",
pages = "1805-1811",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2859"
}

Zdunić, G., Gođevac, D., Šavikin, K.,& Petrović, S.. (2017). Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 12(11), 1805-1811.
https://hdl.handle.net/21.15107/rcub_farfar_2859

Zdunić G, Gođevac D, Šavikin K, Petrović S. Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. in Natural Product Communications. 2017;12(11):1805-1811.
https://hdl.handle.net/21.15107/rcub_farfar_2859 .

Zdunić, Gordana, Gođevac, Dejan, Šavikin, Katarina, Petrović, Silvana, "Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties" in Natural Product Communications, 12, no. 11 (2017):1805-1811,
https://hdl.handle.net/21.15107/rcub_farfar_2859 .

TY  - JOUR
AU  - Cujić, Nada
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Pljevljakusić, Dejan
AU  - Zdunić, Gordana
AU  - Ibrić, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2582
AB  - Traditional maceration method was used for the extraction of polyphenols from chokeberry (Aronia melanocarpa) dried fruit, and the effects of several extraction parameters on the total phenolics and anthocyanins contents were studied. Various solvents, particle size, solid solvent ratio and extraction time have been investigated as independent variables in two level factorial design. Among examined variables, time was not statistically important factor for the extraction of polyphenols. The optimal extraction conditions were maceration of 0.75 mm size berries by 50% ethanol, with solid solvent ratio of 1:20, and predicted values were 27.7 mg GAE/g for total phenolics and 0.27% for total anthocyanins. Under selected conditions, the experimental total phenolics were 27.8 mg GAE/g, and total anthocyanins were 0.27%, which is in agreement with the predicted values. In addition, a complementary quantitative analysis of individual phenolic compounds was performed using HPLC method. The study indicated that maceration was effective and simple technique for the extraction of bioactive compounds from chokeberry fruit.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique
VL  - 194
SP  - 135
EP  - 142
DO  - 10.1016/j.foodchem.2015.08.008
ER  - 

@article{
author = "Cujić, Nada and Šavikin, Katarina and Janković, Teodora and Pljevljakusić, Dejan and Zdunić, Gordana and Ibrić, Svetlana",
year = "2016",
abstract = "Traditional maceration method was used for the extraction of polyphenols from chokeberry (Aronia melanocarpa) dried fruit, and the effects of several extraction parameters on the total phenolics and anthocyanins contents were studied. Various solvents, particle size, solid solvent ratio and extraction time have been investigated as independent variables in two level factorial design. Among examined variables, time was not statistically important factor for the extraction of polyphenols. The optimal extraction conditions were maceration of 0.75 mm size berries by 50% ethanol, with solid solvent ratio of 1:20, and predicted values were 27.7 mg GAE/g for total phenolics and 0.27% for total anthocyanins. Under selected conditions, the experimental total phenolics were 27.8 mg GAE/g, and total anthocyanins were 0.27%, which is in agreement with the predicted values. In addition, a complementary quantitative analysis of individual phenolic compounds was performed using HPLC method. The study indicated that maceration was effective and simple technique for the extraction of bioactive compounds from chokeberry fruit.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique",
volume = "194",
pages = "135-142",
doi = "10.1016/j.foodchem.2015.08.008"
}

Cujić, N., Šavikin, K., Janković, T., Pljevljakusić, D., Zdunić, G.,& Ibrić, S.. (2016). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. in Food Chemistry
Elsevier Sci Ltd, Oxford., 194, 135-142.
https://doi.org/10.1016/j.foodchem.2015.08.008

Cujić N, Šavikin K, Janković T, Pljevljakusić D, Zdunić G, Ibrić S. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. in Food Chemistry. 2016;194:135-142.
doi:10.1016/j.foodchem.2015.08.008 .

Cujić, Nada, Šavikin, Katarina, Janković, Teodora, Pljevljakusić, Dejan, Zdunić, Gordana, Ibrić, Svetlana, "Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique" in Food Chemistry, 194 (2016):135-142,
https://doi.org/10.1016/j.foodchem.2015.08.008 . .

TY  - JOUR
AU  - Cujić, Nada
AU  - Trifković, Kata
AU  - Bugarski, Branko
AU  - Ibrić, Svetlana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2544
AB  - Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system
VL  - 86
SP  - 120
EP  - 131
DO  - 10.1016/j.indcrop.2016.03.045
ER  - 

@article{
author = "Cujić, Nada and Trifković, Kata and Bugarski, Branko and Ibrić, Svetlana and Pljevljakusić, Dejan and Šavikin, Katarina",
year = "2016",
abstract = "Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system",
volume = "86",
pages = "120-131",
doi = "10.1016/j.indcrop.2016.03.045"
}

Cujić, N., Trifković, K., Bugarski, B., Ibrić, S., Pljevljakusić, D.,& Šavikin, K.. (2016). Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 86, 120-131.
https://doi.org/10.1016/j.indcrop.2016.03.045

Cujić N, Trifković K, Bugarski B, Ibrić S, Pljevljakusić D, Šavikin K. Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products. 2016;86:120-131.
doi:10.1016/j.indcrop.2016.03.045 .

Cujić, Nada, Trifković, Kata, Bugarski, Branko, Ibrić, Svetlana, Pljevljakusić, Dejan, Šavikin, Katarina, "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system" in Industrial Crops and Products, 86 (2016):120-131,
https://doi.org/10.1016/j.indcrop.2016.03.045 . .

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Živković, Jelena
AU  - Tovilović, Gordana
AU  - Šavikin, Katarina
AU  - Tomić, Mirko
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2393
AB  - Methanol and 70% acetone extracts from Veronica jacquinii, Veronica urticifolia and Veronica teucrium were evaluated regarding their neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y cell line. All extracts exhibited modest protective activity by increasing cell survival in cells stressed with sodium nitroprusside (9-12%) and hydrogen peroxide (16-21%) compared to non-treated cells. These activities were accompanied by reductions in the amount of superoxide radicals and index of lipid peroxidation. Extracts were further analysed for the content of total phenolics, phenylpropanoid glycosides and iridoids, and the major compounds acteoside and aucubin. The highest amount of total phenolics was observed in V. jacquinii, while V. teucrium was richest in total iridoids, acteoside and aucubin. Angiogenic properties on EA.hy926 human endothelial cells were also examined. At the highest non-toxic concentration (25 mu g/ml), the tested extracts inhibited spontaneous tube formation of endothelial cells on the extracellular matrix, implying their possible antiangiogenic activity. The most potent inhibitory effects were shown by methanol extract of V. jacquinii and aqueous acetone extract of V. teucrium. The extracts did not significantly change the adhesive or migratory capacity of EA.hy926 cells. Considering the traditional use of Veronica species, these results suggest a need for further assessment of their supposed wound-healing properties.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Frontiers in Life Science
T1  - Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species
VL  - 8
IS  - 2
SP  - 107
EP  - 116
DO  - 10.1080/21553769.2014.998297
ER  - 

@article{
author = "Ignjatović, Đurđica and Živković, Jelena and Tovilović, Gordana and Šavikin, Katarina and Tomić, Mirko and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Methanol and 70% acetone extracts from Veronica jacquinii, Veronica urticifolia and Veronica teucrium were evaluated regarding their neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y cell line. All extracts exhibited modest protective activity by increasing cell survival in cells stressed with sodium nitroprusside (9-12%) and hydrogen peroxide (16-21%) compared to non-treated cells. These activities were accompanied by reductions in the amount of superoxide radicals and index of lipid peroxidation. Extracts were further analysed for the content of total phenolics, phenylpropanoid glycosides and iridoids, and the major compounds acteoside and aucubin. The highest amount of total phenolics was observed in V. jacquinii, while V. teucrium was richest in total iridoids, acteoside and aucubin. Angiogenic properties on EA.hy926 human endothelial cells were also examined. At the highest non-toxic concentration (25 mu g/ml), the tested extracts inhibited spontaneous tube formation of endothelial cells on the extracellular matrix, implying their possible antiangiogenic activity. The most potent inhibitory effects were shown by methanol extract of V. jacquinii and aqueous acetone extract of V. teucrium. The extracts did not significantly change the adhesive or migratory capacity of EA.hy926 cells. Considering the traditional use of Veronica species, these results suggest a need for further assessment of their supposed wound-healing properties.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Frontiers in Life Science",
title = "Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species",
volume = "8",
number = "2",
pages = "107-116",
doi = "10.1080/21553769.2014.998297"
}

Ignjatović, Đ., Živković, J., Tovilović, G., Šavikin, K., Tomić, M., Maksimović, Z.,& Janković, T.. (2015). Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species. in Frontiers in Life Science
Taylor & Francis Ltd, Abingdon., 8(2), 107-116.
https://doi.org/10.1080/21553769.2014.998297

Ignjatović Đ, Živković J, Tovilović G, Šavikin K, Tomić M, Maksimović Z, Janković T. Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species. in Frontiers in Life Science. 2015;8(2):107-116.
doi:10.1080/21553769.2014.998297 .

Ignjatović, Đurđica, Živković, Jelena, Tovilović, Gordana, Šavikin, Katarina, Tomić, Mirko, Maksimović, Zoran, Janković, Teodora, "Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species" in Frontiers in Life Science, 8, no. 2 (2015):107-116,
https://doi.org/10.1080/21553769.2014.998297 . .

TY  - JOUR
AU  - Beara, Ivana
AU  - Živković, Jelena
AU  - Lesjak, Marija
AU  - Ristić, Jelena
AU  - Šavikin, Katarina
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2377
AB  - Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profile and anti-inflammatory activity of three Veronica species
VL  - 63
SP  - 276
EP  - 280
DO  - 10.1016/j.indcrop.2014.09.034
ER  - 

@article{
author = "Beara, Ivana and Živković, Jelena and Lesjak, Marija and Ristić, Jelena and Šavikin, Katarina and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profile and anti-inflammatory activity of three Veronica species",
volume = "63",
pages = "276-280",
doi = "10.1016/j.indcrop.2014.09.034"
}

Beara, I., Živković, J., Lesjak, M., Ristić, J., Šavikin, K., Maksimović, Z.,& Janković, T.. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 63, 276-280.
https://doi.org/10.1016/j.indcrop.2014.09.034

Beara I, Živković J, Lesjak M, Ristić J, Šavikin K, Maksimović Z, Janković T. Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products. 2015;63:276-280.
doi:10.1016/j.indcrop.2014.09.034 .

Beara, Ivana, Živković, Jelena, Lesjak, Marija, Ristić, Jelena, Šavikin, Katarina, Maksimović, Zoran, Janković, Teodora, "Phenolic profile and anti-inflammatory activity of three Veronica species" in Industrial Crops and Products, 63 (2015):276-280,
https://doi.org/10.1016/j.indcrop.2014.09.034 . .

TY  - JOUR
AU  - Ćujić, Nada
AU  - Ibrić, Svetlana
AU  - Bigović, Dubravka
AU  - Noveski, Nebojša
AU  - Šavikin, Katarina
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2482
AB  - Nowdays, the use of products based on medicinal herbs (phytopreparates) are in scientific focus, both in prevention or in treatment of various diseases. Herbal products have been recognized as an excellent source of bioactive compounds which have positive effects on human health. Beside teas, as standard herbal products, phytopreparations are much more represented as final pharmaceutical form on the market today. Phytopreparations must satisfy standard quality, which means that they must be physically, chemically and microbiological stable and have a high degree of purity. In order to determine the stability, storage conditions, shelf-life of the products, stability tests are conducted, which involving tests of environmental factors influence: temperature, relative humidity, light. Stability tests are performed at different stages of development and production. In accordance with the EMEA (European Agency for Drugs and Medical Devices) for a variety of herbal preparations different specific stability tests are conducted.
AB  - Danas, sve je veći akcenat na upotrebi preparata na bazi lekovitog bilja (fitopreparata), kako u prevenciji tako i u lečenju različitih oboljenja. Biljni preparati su prepoznati kao odličan izvor bioaktivnih komponenti, koji imaju pozitivne efekte na zdravlje ljudi. Pored čajeva, kao standardnih biljnih proizvoda, danas su na tržištu mnogo više zastupljeni fitopreparati kao finalne farmaceutske forme. Fitopreparati moraju biti standardnog kvaliteta, što podrazumeva da moraju biti fizički, hemijski i mikrobilološki stabilni, da su standardizovanog sastava i da su visokog stepena čistoće. U cilju utvrđivanja koliko je jedan proizvod stabilan, definisanja uslova čuvanja, određivanja roka trajanja, sprovode se ispitivanja stabilnosti, koja podrazumevaju ispitivanja uticaja faktora okoline: temperature, relativne vlažnosti vazduha, svetlosti na promenu kvaliteta gotovog proizvoda. Ispitivanja stabilnosti se izvode u različitim fazama razvoja i proizvodnje. U skladu sa zahtevima EMEA (Evropska agencija za lekove i medicinska sredstva) za različite biljne preparate postavljaju se različiti specifični testovi stabilnosti.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Stability testing of herbal medicines
T1  - Ispitivanje stabilnosti biljnih preparata
IS  - 35
SP  - 53
EP  - 60
DO  - 10.5937/leksir1535053C
ER  - 

@article{
author = "Ćujić, Nada and Ibrić, Svetlana and Bigović, Dubravka and Noveski, Nebojša and Šavikin, Katarina",
year = "2015",
abstract = "Nowdays, the use of products based on medicinal herbs (phytopreparates) are in scientific focus, both in prevention or in treatment of various diseases. Herbal products have been recognized as an excellent source of bioactive compounds which have positive effects on human health. Beside teas, as standard herbal products, phytopreparations are much more represented as final pharmaceutical form on the market today. Phytopreparations must satisfy standard quality, which means that they must be physically, chemically and microbiological stable and have a high degree of purity. In order to determine the stability, storage conditions, shelf-life of the products, stability tests are conducted, which involving tests of environmental factors influence: temperature, relative humidity, light. Stability tests are performed at different stages of development and production. In accordance with the EMEA (European Agency for Drugs and Medical Devices) for a variety of herbal preparations different specific stability tests are conducted., Danas, sve je veći akcenat na upotrebi preparata na bazi lekovitog bilja (fitopreparata), kako u prevenciji tako i u lečenju različitih oboljenja. Biljni preparati su prepoznati kao odličan izvor bioaktivnih komponenti, koji imaju pozitivne efekte na zdravlje ljudi. Pored čajeva, kao standardnih biljnih proizvoda, danas su na tržištu mnogo više zastupljeni fitopreparati kao finalne farmaceutske forme. Fitopreparati moraju biti standardnog kvaliteta, što podrazumeva da moraju biti fizički, hemijski i mikrobilološki stabilni, da su standardizovanog sastava i da su visokog stepena čistoće. U cilju utvrđivanja koliko je jedan proizvod stabilan, definisanja uslova čuvanja, određivanja roka trajanja, sprovode se ispitivanja stabilnosti, koja podrazumevaju ispitivanja uticaja faktora okoline: temperature, relativne vlažnosti vazduha, svetlosti na promenu kvaliteta gotovog proizvoda. Ispitivanja stabilnosti se izvode u različitim fazama razvoja i proizvodnje. U skladu sa zahtevima EMEA (Evropska agencija za lekove i medicinska sredstva) za različite biljne preparate postavljaju se različiti specifični testovi stabilnosti.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Stability testing of herbal medicines, Ispitivanje stabilnosti biljnih preparata",
number = "35",
pages = "53-60",
doi = "10.5937/leksir1535053C"
}

Ćujić, N., Ibrić, S., Bigović, D., Noveski, N.,& Šavikin, K.. (2015). Stability testing of herbal medicines. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(35), 53-60.
https://doi.org/10.5937/leksir1535053C

Ćujić N, Ibrić S, Bigović D, Noveski N, Šavikin K. Stability testing of herbal medicines. in Lekovite sirovine. 2015;(35):53-60.
doi:10.5937/leksir1535053C .

Ćujić, Nada, Ibrić, Svetlana, Bigović, Dubravka, Noveski, Nebojša, Šavikin, Katarina, "Stability testing of herbal medicines" in Lekovite sirovine, no. 35 (2015):53-60,
https://doi.org/10.5937/leksir1535053C . .

TY  - JOUR
AU  - Ćujić, Nada
AU  - Ibrić, Svetlana
AU  - Bigović, Dubravka
AU  - Noveski, Nebojša
AU  - Šavikin, Katarina
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2481
AB  - In recent years, drug delivery systems are especially interesting because they allow therapeutic efficiency and reduce side effects. Conjugates of hyaluronic acid and chemotherapeutic agents, especially taxol, have been recognized as excellent solution for many therapeutic indications. The aim of conjugation of taxol with hyaluronic acid was the toxicity reduction on healthy cells and increase of selectivity on tumor cells. Hyaluronic acid could be used in various pharmaceutical preparations, also in parenteral, because of its biocompatibility and biodegradability. Hyaluronic acid as a carrier belongs to in situ gelling systems, which is passing a phase transition in response to external conditions such as temperature, different pH value or the presence of ions, enabling prolonged effect of the drug substances. Many tumor cells have a large number of receptors for hyaluronic acid, which has a great affinity for the tumor cells. The incorporation of taxol in gelling system of hyaluronic acid provides sustained drug release, increased anticancer activity, reduced damage of healthy cells and increased selectivity on cancer cells.
AB  - Preparati sa produženim oslobađanjem su poslednjih godina posebno aktuelni jer omogućavaju veću terapijsku efikasnost, smanjuju pojavu neželjenih efekata i omogućavaju ciljano delovanje. Posebno su poslednjih godina interesantni konjugati hijaluronske kiseline i citostatika, a jedan od njih je i taksol, koji pored mnogobrojnih terapijskih indikacija ispoljava i niz neželjenih efekata. Cilj konjugovanja taksola sa hijaluronskom kiselinom je smanjenje toksičnosti na zdravim i povećanje selektivnosti na tumorskim ćelijama. Hijaluronska kiselina se može primenjivati u različitim farmaceutskim preparatima, pa i u parenteralnim, jer ima niz prednosti kao što su biokompatibilnost i biodegradibilnost. Spada u in situ gelirajuće sisteme koji se podvrgavaju faznom prelazu u odgovoru na spoljne stimulanse kao što su temperatura, pH ili prisustvo jona, čime se omogućava produženo dejstvo lekovite supstance. Mnoge tumorske ćelije pokazuju ekspresiju velikog broja receptora za hijaluronsku kiselinu, pa ona ima veliki afinitet za tumorske ćelije. Inkorporiranjem taksola u gelirajući sistem sa hijaluronskom kiselinom omogućava se postepeno oslobađanje leka, povećanje antikancerskog delovanja, smanjuje se oštećenje zdravih ćelija i povećava selektivnost leka.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Pharamaceutical hyaluronic acid based carriers for parenteral use, particularly conjugates with taxol: A review
T1  - Nosači/preparati na bazi hijaluronske kiseline za parenteralnu primenu sa posebnim osvrtom na konjugaciju sa taksolom
IS  - 35
SP  - 75
EP  - 87
DO  - 10.5937/leksir1535075C
ER  - 

@article{
author = "Ćujić, Nada and Ibrić, Svetlana and Bigović, Dubravka and Noveski, Nebojša and Šavikin, Katarina",
year = "2015",
abstract = "In recent years, drug delivery systems are especially interesting because they allow therapeutic efficiency and reduce side effects. Conjugates of hyaluronic acid and chemotherapeutic agents, especially taxol, have been recognized as excellent solution for many therapeutic indications. The aim of conjugation of taxol with hyaluronic acid was the toxicity reduction on healthy cells and increase of selectivity on tumor cells. Hyaluronic acid could be used in various pharmaceutical preparations, also in parenteral, because of its biocompatibility and biodegradability. Hyaluronic acid as a carrier belongs to in situ gelling systems, which is passing a phase transition in response to external conditions such as temperature, different pH value or the presence of ions, enabling prolonged effect of the drug substances. Many tumor cells have a large number of receptors for hyaluronic acid, which has a great affinity for the tumor cells. The incorporation of taxol in gelling system of hyaluronic acid provides sustained drug release, increased anticancer activity, reduced damage of healthy cells and increased selectivity on cancer cells., Preparati sa produženim oslobađanjem su poslednjih godina posebno aktuelni jer omogućavaju veću terapijsku efikasnost, smanjuju pojavu neželjenih efekata i omogućavaju ciljano delovanje. Posebno su poslednjih godina interesantni konjugati hijaluronske kiseline i citostatika, a jedan od njih je i taksol, koji pored mnogobrojnih terapijskih indikacija ispoljava i niz neželjenih efekata. Cilj konjugovanja taksola sa hijaluronskom kiselinom je smanjenje toksičnosti na zdravim i povećanje selektivnosti na tumorskim ćelijama. Hijaluronska kiselina se može primenjivati u različitim farmaceutskim preparatima, pa i u parenteralnim, jer ima niz prednosti kao što su biokompatibilnost i biodegradibilnost. Spada u in situ gelirajuće sisteme koji se podvrgavaju faznom prelazu u odgovoru na spoljne stimulanse kao što su temperatura, pH ili prisustvo jona, čime se omogućava produženo dejstvo lekovite supstance. Mnoge tumorske ćelije pokazuju ekspresiju velikog broja receptora za hijaluronsku kiselinu, pa ona ima veliki afinitet za tumorske ćelije. Inkorporiranjem taksola u gelirajući sistem sa hijaluronskom kiselinom omogućava se postepeno oslobađanje leka, povećanje antikancerskog delovanja, smanjuje se oštećenje zdravih ćelija i povećava selektivnost leka.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Pharamaceutical hyaluronic acid based carriers for parenteral use, particularly conjugates with taxol: A review, Nosači/preparati na bazi hijaluronske kiseline za parenteralnu primenu sa posebnim osvrtom na konjugaciju sa taksolom",
number = "35",
pages = "75-87",
doi = "10.5937/leksir1535075C"
}

Ćujić, N., Ibrić, S., Bigović, D., Noveski, N.,& Šavikin, K.. (2015). Pharamaceutical hyaluronic acid based carriers for parenteral use, particularly conjugates with taxol: A review. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(35), 75-87.
https://doi.org/10.5937/leksir1535075C

Ćujić N, Ibrić S, Bigović D, Noveski N, Šavikin K. Pharamaceutical hyaluronic acid based carriers for parenteral use, particularly conjugates with taxol: A review. in Lekovite sirovine. 2015;(35):75-87.
doi:10.5937/leksir1535075C .

Ćujić, Nada, Ibrić, Svetlana, Bigović, Dubravka, Noveski, Nebojša, Šavikin, Katarina, "Pharamaceutical hyaluronic acid based carriers for parenteral use, particularly conjugates with taxol: A review" in Lekovite sirovine, no. 35 (2015):75-87,
https://doi.org/10.5937/leksir1535075C . .

TY  - JOUR
AU  - Kardum, Nevena
AU  - Konić-Ristić, Aleksandra
AU  - Šavikin, Katarina
AU  - Spasić, Slavica
AU  - Stefanović, Aleksandra
AU  - Ivanišević, Jasmina
AU  - Miljković, Milica
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2100
AB  - Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P  lt  .001) and pro-oxidant-antioxidant balance (PAB; P  lt  .05), as well as increase in paroxonase-1 activity toward diazoxon (P  lt  .01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro-oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects
VL  - 17
IS  - 8
SP  - 869
EP  - 874
DO  - 10.1089/jmf.2013.0135
ER  - 

@article{
author = "Kardum, Nevena and Konić-Ristić, Aleksandra and Šavikin, Katarina and Spasić, Slavica and Stefanović, Aleksandra and Ivanišević, Jasmina and Miljković, Milica",
year = "2014",
abstract = "Berry fruits are a rich source of polyphenols, especially anthocyanins: well-known potent anti-oxidant phytochemicals. The purpose of this study was to evaluate beneficial effects of long-term consumption of polyphenol-rich organic chokeberry juice on different markers of antioxidant/pro-oxidant status in healthy female volunteers. Twenty-nine women, aged 25-49, were included in the study. Serological markers of oxidative stress and antioxidant defence, blood pressure, routine biochemical, and anthropometric parameters were analyzed at baseline and after twelve weeks of regular chokeberry juice consumption. Significant decrease in thiobarbituric acid-reactive substances level (TBARS; P  lt  .001) and pro-oxidant-antioxidant balance (PAB; P  lt  .05), as well as increase in paroxonase-1 activity toward diazoxon (P  lt  .01) were found. Total oxidative status and sulphydryl groups levels were not significantly influenced by the intervention. Anthropometric, biochemical parameters, and blood pressure values were within the referent values for all subjects and were not influenced by the chokeberry juice consumption. However, we found positive correlation between age, body mass index, waist circumference, body fat percent, blood pressure, and analyzed marker of lipid peroxidation, which was influenced by the consumption. In conclusion, the fine modulation of several antioxidant/pro-oxidant status biomarkers observed in healthy subjects indicates putative prophylactic effects of polyphenol-rich chokeberry juice and supports its importance as part of an optimal diet.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects",
volume = "17",
number = "8",
pages = "869-874",
doi = "10.1089/jmf.2013.0135"
}

Kardum, N., Konić-Ristić, A., Šavikin, K., Spasić, S., Stefanović, A., Ivanišević, J.,& Miljković, M.. (2014). Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 17(8), 869-874.
https://doi.org/10.1089/jmf.2013.0135

Kardum N, Konić-Ristić A, Šavikin K, Spasić S, Stefanović A, Ivanišević J, Miljković M. Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects. in Journal of Medicinal Food. 2014;17(8):869-874.
doi:10.1089/jmf.2013.0135 .

Kardum, Nevena, Konić-Ristić, Aleksandra, Šavikin, Katarina, Spasić, Slavica, Stefanović, Aleksandra, Ivanišević, Jasmina, Miljković, Milica, "Effects of Polyphenol-Rich Chokeberry Juice on Antioxidant/Pro-Oxidant Status in Healthy Subjects" in Journal of Medicinal Food, 17, no. 8 (2014):869-874,
https://doi.org/10.1089/jmf.2013.0135 . .

TY  - JOUR
AU  - Ćujić, Nada
AU  - Kundaković, Tatjana
AU  - Šavikin, Katarina
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2018
AB  - Anthocyanins are a group of over 500 different compounds that cause red, purple and blue colours of many plants, especially fruits, vegetables and cereals. Anthocyanins structurally belong to flavones. Mainly fruit is considered the main source of anthocyanins, although they are present in vegetables, roots, bulbs, fruit pulps, pulses and cereals. Huge diversity of anthocyanins, which can be found in nature makes them very complex and interesting group. In plants anthocyanins are present in the form of heterosides. Aglycone anthocyanins are known as anthocyanins. In nature there is 6 well known anthocyanins: pelargonidin, cyanidin, peonidin, delphinidin, malvidin and petunidin. Pelargonidin as stable anthocyanins is the most frequent in nature. The glycoside derivatives of the most widespread in nature are 3-monoside, 3.5 and 3.7 biosides. Interest for anthocyanins is growing over the past few years due to their potential applications in the pharmaceutical industry, the food and cosmetic industries. Despite their great potential, their use is still limited because of their relative instability and the low percentage of extracting from the plant material. Anthocyanins are quite reactive and unstable compounds. They transform reversibly with pH changes. pH is defined as the greatest cause of instability of anthocyanins, and has the greatest impact on colour. Anthocyanins are polar molecules and the most efficient solvent for extraction are the mixture of polar solvent such as ethanol, methanol and acetone. Extraction methods for anthocyanins isolation are not selective because solvents extracted anthocyanins with ballast and the great number of substances such as sugars and organic acids. Because of that, it is necessary to implement a new purification techniques, such as solid phase extraction (SPE), liquid-liquid extraction (LLE), countercurrent chromatography (CCC), and high performance liquid chromatography (HPLC). Identification of anthocyanins play a key role in the taxonomy and evaluate the quality of herbal drugs and foods containing anthocyanins. Reversed phase RP- HPLC connected to a photodiode (DAD detector) is the most used method for detection and identification of anthocyanins. Sample preparation for HPLC analysis usually involves acid hydrolysis of anthocyanins to release anthocyanidins. Anthocyanins may be quantified using any purified standard, but most commonly used is standard of cyanidin-3-glucoside, and quantification is carried out at a wavelength of 520 nm. The method of choice for the rapid screening of total anthocyanins in vegetables and fruits is spectrophotometric determination at 528 nm, with a controlled pH. This method works very well, when the assessment is required and not the accuracy of the quantification. Anthocyanins are poorly absorbed and metabolised to the same extent as other flavonoids, which are considered to have low bioavailability. Examination of bioavailability for most of the anthocyanins was carried out on animals. Most studies have shown maximum concentration in blood after 15min-2h. Interest in anthocyanins has especially grown in recent years due to their positive effects on health. Anthocyanins are effective for chronic diseases, especially in cardiovascular diseases. Together with the other nutritional ingredients are important because of their antioxidant ability, suggesting their potential application in the prevention of several diseases which are associated with oxidative stress. Some plants with high content of anthocyanins play a role in the prevention of mutagenesis and carcinogenesis as inhibitors. Extracts obtained from berries have the highest potential for the removal free radicals. Antioxidant activity of them is proportional to the content of anthocyanins.
AB  - Antocijani su grupa od preko 500 različitih jedinjenja koja uzrokuju crvenu, ljubičastu i plavu boju mnogih biljaka, a posebno voća, povrća i žitarica. Antocijani strukturno pripadaju flavonima. Uglavnom se voće smatra glavnim izvorom antocijana, mada su oni prisutni i u povrću, korenju, lukovicama, pulpama plodova, mahunarkama i žitaricama. Ogromna raznovrsnost antocijana koja može da se nađe u prirodi čini ih veoma složenom i interesantnom grupom. U biljnim vrstama su prisutni u vidu heterozida. Aglikoni antocijana poznati su kao antocijanidini, a u prirodi je do sad poznato 6 antocijanidina: pelargonidin, cijanidin, peonidin, delfinidin, petunidin i malvidin. Pelargonidin kao najstabilniji antocijanidin je i najviše zastupljen u prirodi. U glikozidne derivate najviše rasprostranjene u prirodi spadaju 3-monozidi, 3,5 biozidi i 3,7 diglukozidi. Interesovanje za antocijane je sve veće proteklih par godina, zbog njihove potencijalne primene u farmaceutskoj industriji, industriji hrane i kozmetičkoj industriji. Uprkos tome njihova upotreba je i dalje ograničena zbog njihove relativne nestabilnosti i niskom procentu ekstrahovanja iz biljnog materijala. Antocijani su reaktivna i dosta nestabilna jedinjenja. Reverzibilno trpe strukturne transformacije sa promenom pH što ima najveći uticaj na boju. pH je određen kao uzročnik najveće nestabilnosti antocijana, i od pH u najvećoj meri zavisi njihova pigmentacija. Antocijani su polarni molekuli tako da su se kao najefikasniji rastvarači za ekstrakciju pokazale smeše polarnih rastvarača kao što su etanol, metanol i aceton. Metode ekstrakcije koje se koriste za izolovanje antocijana nisu selektivne jer pored antocijana ekstrahuje se i veliki broj balastnih materija kao što su šećeri i organske kiseline. Neophodno je implementirati nove tehnike prečišćavanja, kao što su ekstrakcije na čvrstoj fazi (SPE), ekstrakcije tečno-tečno (LLE), kao i korišćenje sofisticiranih hromatografskih tehnika kao što su protivstrujna hromatografija (CCC), i visoko efikasna tečna hromatografija (HPLC). Identifikacija antocijana ima ključnu ulogu u taksonomiji, i proceni kvaliteta biljnih droga i hrane koja sadrži antocijane. Reverzno fazna RP-HPLC povezana sa fotodiodom (DAD detektor) je najviše korišćena metoda za identifikaciju i određivanje antocijana. Priprema uzoraka za HPLC analizu uglavnom podrazumeva kiselu hidrolizu antocijana da bi se oslobodili antocijanidini. Antocijani mogu biti kvantifikovani preko bilo kog prečišćenog standarda, ali najčešće se kao standard koristi cijanidin-3-glikozid, a kvantifikacija se vrši na talasnoj dužini od 520 nm. Metoda izbora za brz skrining ukupnih antocijana u povrću i voću je spektrofotometrijsko određivanje na 528 nm, pri kontrolisanoj pH. Ova metoda funkcioniše veoma dobro gde je potrebna procena, a ne preciznost kvantifikacije. Antocijani se slabo resorbuju i ne metabolišu se u istom stepenu kao drugi flavonoidi, zbog čega se smatra da imaju malu bioraspoloživost. Ispitivanje bioraspoloživosti antocijana uglavnom je vršeno je na životinjama. Većina studija je pokazala da dostižu maksimalnu koncentraciju u krvi nakon 15 min - 2h. Zainteresovanost za antocijane je posebno porasla poslednjih godina zbog njihovih pozitivnih efekata na zdravlje. Deluju kod hroničnih bolesti, a u prvom redu kod kardiovaskularnih oboljenja. Oni su zajedno sa ostalim flavonoidima važni nutritivni sastojci zbog svojih antioksidativnih sposobnosti, sto ukazuje na njihovu potencijalnu primenu u prevenciji nekoliko bolesti koje su povezane sa oksidativnim stresom. Neke biljke sa visokim sadržajem antocijana imaju ulogu u prevenciji mutageneze i inhibitori su kancerogeneze. Ekstrakti dobijeni iz bobičastog voća imaju najveći potencijal uklanjanja slobodnih radikala i kod njih je antioksidativna aktivnost proporcionalna sadržaju antocijana.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Anthocyanins: Chemistry and biological activity
T1  - Antocijani - hemijska analiza i biološka aktivnost
IS  - 33
SP  - 19
EP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2018
ER  - 

@article{
author = "Ćujić, Nada and Kundaković, Tatjana and Šavikin, Katarina",
year = "2013",
abstract = "Anthocyanins are a group of over 500 different compounds that cause red, purple and blue colours of many plants, especially fruits, vegetables and cereals. Anthocyanins structurally belong to flavones. Mainly fruit is considered the main source of anthocyanins, although they are present in vegetables, roots, bulbs, fruit pulps, pulses and cereals. Huge diversity of anthocyanins, which can be found in nature makes them very complex and interesting group. In plants anthocyanins are present in the form of heterosides. Aglycone anthocyanins are known as anthocyanins. In nature there is 6 well known anthocyanins: pelargonidin, cyanidin, peonidin, delphinidin, malvidin and petunidin. Pelargonidin as stable anthocyanins is the most frequent in nature. The glycoside derivatives of the most widespread in nature are 3-monoside, 3.5 and 3.7 biosides. Interest for anthocyanins is growing over the past few years due to their potential applications in the pharmaceutical industry, the food and cosmetic industries. Despite their great potential, their use is still limited because of their relative instability and the low percentage of extracting from the plant material. Anthocyanins are quite reactive and unstable compounds. They transform reversibly with pH changes. pH is defined as the greatest cause of instability of anthocyanins, and has the greatest impact on colour. Anthocyanins are polar molecules and the most efficient solvent for extraction are the mixture of polar solvent such as ethanol, methanol and acetone. Extraction methods for anthocyanins isolation are not selective because solvents extracted anthocyanins with ballast and the great number of substances such as sugars and organic acids. Because of that, it is necessary to implement a new purification techniques, such as solid phase extraction (SPE), liquid-liquid extraction (LLE), countercurrent chromatography (CCC), and high performance liquid chromatography (HPLC). Identification of anthocyanins play a key role in the taxonomy and evaluate the quality of herbal drugs and foods containing anthocyanins. Reversed phase RP- HPLC connected to a photodiode (DAD detector) is the most used method for detection and identification of anthocyanins. Sample preparation for HPLC analysis usually involves acid hydrolysis of anthocyanins to release anthocyanidins. Anthocyanins may be quantified using any purified standard, but most commonly used is standard of cyanidin-3-glucoside, and quantification is carried out at a wavelength of 520 nm. The method of choice for the rapid screening of total anthocyanins in vegetables and fruits is spectrophotometric determination at 528 nm, with a controlled pH. This method works very well, when the assessment is required and not the accuracy of the quantification. Anthocyanins are poorly absorbed and metabolised to the same extent as other flavonoids, which are considered to have low bioavailability. Examination of bioavailability for most of the anthocyanins was carried out on animals. Most studies have shown maximum concentration in blood after 15min-2h. Interest in anthocyanins has especially grown in recent years due to their positive effects on health. Anthocyanins are effective for chronic diseases, especially in cardiovascular diseases. Together with the other nutritional ingredients are important because of their antioxidant ability, suggesting their potential application in the prevention of several diseases which are associated with oxidative stress. Some plants with high content of anthocyanins play a role in the prevention of mutagenesis and carcinogenesis as inhibitors. Extracts obtained from berries have the highest potential for the removal free radicals. Antioxidant activity of them is proportional to the content of anthocyanins., Antocijani su grupa od preko 500 različitih jedinjenja koja uzrokuju crvenu, ljubičastu i plavu boju mnogih biljaka, a posebno voća, povrća i žitarica. Antocijani strukturno pripadaju flavonima. Uglavnom se voće smatra glavnim izvorom antocijana, mada su oni prisutni i u povrću, korenju, lukovicama, pulpama plodova, mahunarkama i žitaricama. Ogromna raznovrsnost antocijana koja može da se nađe u prirodi čini ih veoma složenom i interesantnom grupom. U biljnim vrstama su prisutni u vidu heterozida. Aglikoni antocijana poznati su kao antocijanidini, a u prirodi je do sad poznato 6 antocijanidina: pelargonidin, cijanidin, peonidin, delfinidin, petunidin i malvidin. Pelargonidin kao najstabilniji antocijanidin je i najviše zastupljen u prirodi. U glikozidne derivate najviše rasprostranjene u prirodi spadaju 3-monozidi, 3,5 biozidi i 3,7 diglukozidi. Interesovanje za antocijane je sve veće proteklih par godina, zbog njihove potencijalne primene u farmaceutskoj industriji, industriji hrane i kozmetičkoj industriji. Uprkos tome njihova upotreba je i dalje ograničena zbog njihove relativne nestabilnosti i niskom procentu ekstrahovanja iz biljnog materijala. Antocijani su reaktivna i dosta nestabilna jedinjenja. Reverzibilno trpe strukturne transformacije sa promenom pH što ima najveći uticaj na boju. pH je određen kao uzročnik najveće nestabilnosti antocijana, i od pH u najvećoj meri zavisi njihova pigmentacija. Antocijani su polarni molekuli tako da su se kao najefikasniji rastvarači za ekstrakciju pokazale smeše polarnih rastvarača kao što su etanol, metanol i aceton. Metode ekstrakcije koje se koriste za izolovanje antocijana nisu selektivne jer pored antocijana ekstrahuje se i veliki broj balastnih materija kao što su šećeri i organske kiseline. Neophodno je implementirati nove tehnike prečišćavanja, kao što su ekstrakcije na čvrstoj fazi (SPE), ekstrakcije tečno-tečno (LLE), kao i korišćenje sofisticiranih hromatografskih tehnika kao što su protivstrujna hromatografija (CCC), i visoko efikasna tečna hromatografija (HPLC). Identifikacija antocijana ima ključnu ulogu u taksonomiji, i proceni kvaliteta biljnih droga i hrane koja sadrži antocijane. Reverzno fazna RP-HPLC povezana sa fotodiodom (DAD detektor) je najviše korišćena metoda za identifikaciju i određivanje antocijana. Priprema uzoraka za HPLC analizu uglavnom podrazumeva kiselu hidrolizu antocijana da bi se oslobodili antocijanidini. Antocijani mogu biti kvantifikovani preko bilo kog prečišćenog standarda, ali najčešće se kao standard koristi cijanidin-3-glikozid, a kvantifikacija se vrši na talasnoj dužini od 520 nm. Metoda izbora za brz skrining ukupnih antocijana u povrću i voću je spektrofotometrijsko određivanje na 528 nm, pri kontrolisanoj pH. Ova metoda funkcioniše veoma dobro gde je potrebna procena, a ne preciznost kvantifikacije. Antocijani se slabo resorbuju i ne metabolišu se u istom stepenu kao drugi flavonoidi, zbog čega se smatra da imaju malu bioraspoloživost. Ispitivanje bioraspoloživosti antocijana uglavnom je vršeno je na životinjama. Većina studija je pokazala da dostižu maksimalnu koncentraciju u krvi nakon 15 min - 2h. Zainteresovanost za antocijane je posebno porasla poslednjih godina zbog njihovih pozitivnih efekata na zdravlje. Deluju kod hroničnih bolesti, a u prvom redu kod kardiovaskularnih oboljenja. Oni su zajedno sa ostalim flavonoidima važni nutritivni sastojci zbog svojih antioksidativnih sposobnosti, sto ukazuje na njihovu potencijalnu primenu u prevenciji nekoliko bolesti koje su povezane sa oksidativnim stresom. Neke biljke sa visokim sadržajem antocijana imaju ulogu u prevenciji mutageneze i inhibitori su kancerogeneze. Ekstrakti dobijeni iz bobičastog voća imaju najveći potencijal uklanjanja slobodnih radikala i kod njih je antioksidativna aktivnost proporcionalna sadržaju antocijana.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Anthocyanins: Chemistry and biological activity, Antocijani - hemijska analiza i biološka aktivnost",
number = "33",
pages = "19-37",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2018"
}

Ćujić, N., Kundaković, T.,& Šavikin, K.. (2013). Anthocyanins: Chemistry and biological activity. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(33), 19-37.
https://hdl.handle.net/21.15107/rcub_farfar_2018

Ćujić N, Kundaković T, Šavikin K. Anthocyanins: Chemistry and biological activity. in Lekovite sirovine. 2013;(33):19-37.
https://hdl.handle.net/21.15107/rcub_farfar_2018 .

Ćujić, Nada, Kundaković, Tatjana, Šavikin, Katarina, "Anthocyanins: Chemistry and biological activity" in Lekovite sirovine, no. 33 (2013):19-37,
https://hdl.handle.net/21.15107/rcub_farfar_2018 .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Šavikin, Katarina
AU  - Novaković, Miroslav
AU  - Milosavljević, Slobodan
AU  - Petrović, Silvana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1542
AB  - The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity
VL  - 25
IS  - 3
SP  - 175
EP  - 187
DO  - 10.1080/14786410802401390
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Šavikin, Katarina and Novaković, Miroslav and Milosavljević, Slobodan and Petrović, Silvana",
year = "2011",
abstract = "The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity",
volume = "25",
number = "3",
pages = "175-187",
doi = "10.1080/14786410802401390"
}

Zdunić, G., Gođevac, D., Šavikin, K., Novaković, M., Milosavljević, S.,& Petrović, S.. (2011). Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 25(3), 175-187.
https://doi.org/10.1080/14786410802401390

Zdunić G, Gođevac D, Šavikin K, Novaković M, Milosavljević S, Petrović S. Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research. 2011;25(3):175-187.
doi:10.1080/14786410802401390 .

Zdunić, Gordana, Gođevac, Dejan, Šavikin, Katarina, Novaković, Miroslav, Milosavljević, Slobodan, Petrović, Silvana, "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity" in Natural Product Research, 25, no. 3 (2011):175-187,
https://doi.org/10.1080/14786410802401390 . .

TY  - JOUR
AU  - Konić-Ristić, Aleksandra
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Juranić, Zorica
AU  - Menković, Nebojša
AU  - Stanković, Ivan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1497
AB  - Total phenolics total anthocyanins mineral content radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries Total phenolic content ranged from 133 0 to 260 3 mg of gallic acid equivalents/100 g of fresh weight for red currant and black currant respectively Bilberry Juice contained the highest amount of total anthocyanins (0 18%) Significant correlation between total phenolics content and radical scavenging activity was observed (r = -0980 p  lt  0 01) All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10 2 to 70 5 mu l/ml Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa Fem X LS 174 MCF-7 and PC-3) Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells Fem X cells and MCF-7 cells were noticed Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Biological activity and chemical composition of different berry juices
VL  - 125
IS  - 4
SP  - 1412
EP  - 1417
DO  - 10.1016/j.foodchem.2010.10.018
ER  - 

@article{
author = "Konić-Ristić, Aleksandra and Šavikin, Katarina and Zdunić, Gordana and Janković, Teodora and Juranić, Zorica and Menković, Nebojša and Stanković, Ivan",
year = "2011",
abstract = "Total phenolics total anthocyanins mineral content radical scavenging activity and antiproliferative activity against human cancer cell lines were evaluated in fresh pressed juices of five different berries Total phenolic content ranged from 133 0 to 260 3 mg of gallic acid equivalents/100 g of fresh weight for red currant and black currant respectively Bilberry Juice contained the highest amount of total anthocyanins (0 18%) Significant correlation between total phenolics content and radical scavenging activity was observed (r = -0980 p  lt  0 01) All examined juices showed antiproliferative activity in dose-dependent manner with IC50 ranging from 10 2 to 70 5 mu l/ml Black currant juice was the most effective inhibitor of proliferation in all cell lines tested (HeLa Fem X LS 174 MCF-7 and PC-3) Significant correlations of acidity and total anthocyanin content with antiproliferative activity of berry juices on HeLa cells Fem X cells and MCF-7 cells were noticed Berry juices are good sources of some minerals and contribute significantly to daily intake of these micronutrients",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Biological activity and chemical composition of different berry juices",
volume = "125",
number = "4",
pages = "1412-1417",
doi = "10.1016/j.foodchem.2010.10.018"
}

Konić-Ristić, A., Šavikin, K., Zdunić, G., Janković, T., Juranić, Z., Menković, N.,& Stanković, I.. (2011). Biological activity and chemical composition of different berry juices. in Food Chemistry
Elsevier Sci Ltd, Oxford., 125(4), 1412-1417.
https://doi.org/10.1016/j.foodchem.2010.10.018

Konić-Ristić A, Šavikin K, Zdunić G, Janković T, Juranić Z, Menković N, Stanković I. Biological activity and chemical composition of different berry juices. in Food Chemistry. 2011;125(4):1412-1417.
doi:10.1016/j.foodchem.2010.10.018 .

Konić-Ristić, Aleksandra, Šavikin, Katarina, Zdunić, Gordana, Janković, Teodora, Juranić, Zorica, Menković, Nebojša, Stanković, Ivan, "Biological activity and chemical composition of different berry juices" in Food Chemistry, 125, no. 4 (2011):1412-1417,
https://doi.org/10.1016/j.foodchem.2010.10.018 . .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Milenković, Marina
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Petrović, Silvana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1343
AB  - Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4 %) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 mu g/mL) and I3,II8-biapigenin (60 mu g/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts
VL  - 5
IS  - 8
SP  - 1215
EP  - 1218
DO  - 10.1177/1934578X1000500814
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1343
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Milenković, Marina and Šavikin, Katarina and Menković, Nebojša and Petrović, Silvana",
year = "2010",
abstract = "Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4 %) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 mu g/mL) and I3,II8-biapigenin (60 mu g/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts",
volume = "5",
number = "8",
pages = "1215-1218",
doi = "10.1177/1934578X1000500814",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1343"
}

Zdunić, G., Gođevac, D., Milenković, M., Šavikin, K., Menković, N.,& Petrović, S.. (2010). Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts. in Natural Product Communications
Natural Products Inc, Westerville., 5(8), 1215-1218.
https://doi.org/10.1177/1934578X1000500814
https://hdl.handle.net/21.15107/rcub_farfar_1343

Zdunić G, Gođevac D, Milenković M, Šavikin K, Menković N, Petrović S. Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts. in Natural Product Communications. 2010;5(8):1215-1218.
doi:10.1177/1934578X1000500814
https://hdl.handle.net/21.15107/rcub_farfar_1343 .

Zdunić, Gordana, Gođevac, Dejan, Milenković, Marina, Šavikin, Katarina, Menković, Nebojša, Petrović, Silvana, "Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts" in Natural Product Communications, 5, no. 8 (2010):1215-1218,
https://doi.org/10.1177/1934578X1000500814 .,
https://hdl.handle.net/21.15107/rcub_farfar_1343 .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Milenković, Marina
AU  - Vučićević, Dragana
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Petrović, Silvana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1206
AB  - Oil extracts of Hypericum perforatum L. (Oleum Hyperici) were prepared in three different ways according to the prescriptions from traditional medicine. Variability of constituents and biological activity were evaluated in the obtained oil extracts. The carrageenan-induced rat paw edema test has been used for screening the antiinflammatory activity, while the indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. All examined oil extracts possessed antiinflammatory and gastroprotective activity. Among them, the oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath (extract 2), in a dose of 1.25 mL/kg p.o., exhibited the highest antiinflammatory effect (95.24 +/- 11.66%) and gastroprotective activity (gastric damage score of 0.21 +/- 0.12). The same oil extract had the highest content of quercetin and I3,II8-biapigenin (1.29 +/- 9 mu g/mL and 52 +/- 4 mu g/mL, respectively). Quercetin and I3,II8-biapigenin exhibited antiinflammatory activity similar to those of indomethacin as well as significant gastroprotective activity. The results provide evidence for the usage of Oleum Hyperici as an antiinflammatory and gastroprotective agent, Which has been based previously only on ethnopharmacological claims. Copyright
PB  - John Wiley & Sons Ltd, Chichester
T2  - Phytotherapy Research
T1  - Evaluation of Hypericum perforatum Oil Extracts for an Antiinflammatory and Gastroprotective Activity in Rats
VL  - 23
IS  - 11
SP  - 1559
EP  - 1564
DO  - 10.1002/ptr.2809
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Milenković, Marina and Vučićević, Dragana and Šavikin, Katarina and Menković, Nebojša and Petrović, Silvana",
year = "2009",
abstract = "Oil extracts of Hypericum perforatum L. (Oleum Hyperici) were prepared in three different ways according to the prescriptions from traditional medicine. Variability of constituents and biological activity were evaluated in the obtained oil extracts. The carrageenan-induced rat paw edema test has been used for screening the antiinflammatory activity, while the indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. All examined oil extracts possessed antiinflammatory and gastroprotective activity. Among them, the oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath (extract 2), in a dose of 1.25 mL/kg p.o., exhibited the highest antiinflammatory effect (95.24 +/- 11.66%) and gastroprotective activity (gastric damage score of 0.21 +/- 0.12). The same oil extract had the highest content of quercetin and I3,II8-biapigenin (1.29 +/- 9 mu g/mL and 52 +/- 4 mu g/mL, respectively). Quercetin and I3,II8-biapigenin exhibited antiinflammatory activity similar to those of indomethacin as well as significant gastroprotective activity. The results provide evidence for the usage of Oleum Hyperici as an antiinflammatory and gastroprotective agent, Which has been based previously only on ethnopharmacological claims. Copyright",
publisher = "John Wiley & Sons Ltd, Chichester",
journal = "Phytotherapy Research",
title = "Evaluation of Hypericum perforatum Oil Extracts for an Antiinflammatory and Gastroprotective Activity in Rats",
volume = "23",
number = "11",
pages = "1559-1564",
doi = "10.1002/ptr.2809"
}

Zdunić, G., Gođevac, D., Milenković, M., Vučićević, D., Šavikin, K., Menković, N.,& Petrović, S.. (2009). Evaluation of Hypericum perforatum Oil Extracts for an Antiinflammatory and Gastroprotective Activity in Rats. in Phytotherapy Research
John Wiley & Sons Ltd, Chichester., 23(11), 1559-1564.
https://doi.org/10.1002/ptr.2809

Zdunić G, Gođevac D, Milenković M, Vučićević D, Šavikin K, Menković N, Petrović S. Evaluation of Hypericum perforatum Oil Extracts for an Antiinflammatory and Gastroprotective Activity in Rats. in Phytotherapy Research. 2009;23(11):1559-1564.
doi:10.1002/ptr.2809 .

Zdunić, Gordana, Gođevac, Dejan, Milenković, Marina, Vučićević, Dragana, Šavikin, Katarina, Menković, Nebojša, Petrović, Silvana, "Evaluation of Hypericum perforatum Oil Extracts for an Antiinflammatory and Gastroprotective Activity in Rats" in Phytotherapy Research, 23, no. 11 (2009):1559-1564,
https://doi.org/10.1002/ptr.2809 . .

TY  - CONF
AU  - Zdunić, Gordana
AU  - Stević, T.
AU  - Gođevac, Dejan
AU  - Šavikin, Katarina
AU  - Petrović, Silvana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/808
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Investigation of antimicrobial and antioxidant activity of seven Hypericum species
T1  - Ispitivanje antimikrobne i antioksidantne aktivnosti sedam Hypericum vrsta
VL  - 56
IS  - 5
SP  - 946
EP  - 947
UR  - https://hdl.handle.net/21.15107/rcub_farfar_808
ER  - 

@conference{
author = "Zdunić, Gordana and Stević, T. and Gođevac, Dejan and Šavikin, Katarina and Petrović, Silvana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Investigation of antimicrobial and antioxidant activity of seven Hypericum species, Ispitivanje antimikrobne i antioksidantne aktivnosti sedam Hypericum vrsta",
volume = "56",
number = "5",
pages = "946-947",
url = "https://hdl.handle.net/21.15107/rcub_farfar_808"
}

Zdunić, G., Stević, T., Gođevac, D., Šavikin, K.,& Petrović, S.. (2006). Investigation of antimicrobial and antioxidant activity of seven Hypericum species. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 946-947.
https://hdl.handle.net/21.15107/rcub_farfar_808

Zdunić G, Stević T, Gođevac D, Šavikin K, Petrović S. Investigation of antimicrobial and antioxidant activity of seven Hypericum species. in Arhiv za farmaciju. 2006;56(5):946-947.
https://hdl.handle.net/21.15107/rcub_farfar_808 .

Zdunić, Gordana, Stević, T., Gođevac, Dejan, Šavikin, Katarina, Petrović, Silvana, "Investigation of antimicrobial and antioxidant activity of seven Hypericum species" in Arhiv za farmaciju, 56, no. 5 (2006):946-947,
https://hdl.handle.net/21.15107/rcub_farfar_808 .

TY  - JOUR
AU  - Šavikin-Fodulović, Katarina
AU  - Stojanović, Danilo
AU  - Menković, Nebojša
AU  - Krstić, Dijana
AU  - Janković, Teodora
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/306
AB  - Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside).
AB  - Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Phytochemical and anatomic study of plant species Gentiana dinarica Beck
T1  - Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae
IS  - 21
SP  - 31
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_farfar_306
ER  - 

@article{
author = "Šavikin-Fodulović, Katarina and Stojanović, Danilo and Menković, Nebojša and Krstić, Dijana and Janković, Teodora",
year = "2001",
abstract = "Gentiana dinarica Beck. (Gentianaceae) is a rare plant species growing at carbonate soils in subalpine and alpine regions. Acording to the Flora of Serbia G. dinarica is only distributed in Kosovo. In course of our recent pharmacognostic investigations G. dinarica was found at two localities on the mountain Tara, locality Zvezda (1230 m) and Veliki Kraj (1330 m). Plant material (aerial parts and roots) was collected from June to August 2000-2001. Anatomic and phytochemical analyses were carried out. Preliminary screening of the extracts of the roots and aerial parts of G. dinarica showed the presence of secoiridoids, flavonoids and xanthone compounds. From both extracts three secoiridoids were isolated (swertiamarin, gentiopicrin and sweroside)., Vrsta Gentiana dinarica Beck. (Gentianaceae) je retka biljna vrsta koja je u Srbiji, prema Flori Srbije, do sada konstatovana samo na Kosovu. U toku terenskih istraživanja, ovu vrstu smo zabeležili na dva lokaliteta na planini Tari i to lokalitet Zvezda (1230 m) i lokalitet Veliki Kraj (1330 m).Specifičnost anatomske građe korena G. dinarica je slabo odrveneo kislem, i tzv. unutarksilemski floem, odnosno grupice floemskih elemenata u centralnom delu sekundarnog drveta.Preliminarno ispitivanje ekstrakata korena i nadzemnog dela G. dinarica pokazao je prisustvo sekoiridoida, flavonoida i ksantona. Od sekoiridoida, izolovani su svercijamarin, genciopikrin i sverozid.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Phytochemical and anatomic study of plant species Gentiana dinarica Beck, Fitohemijska i anatomska analiza vrste Gentiana dinarica Beck., Gentianaceae",
number = "21",
pages = "31-36",
url = "https://hdl.handle.net/21.15107/rcub_farfar_306"
}

Šavikin-Fodulović, K., Stojanović, D., Menković, N., Krstić, D.,& Janković, T.. (2001). Phytochemical and anatomic study of plant species Gentiana dinarica Beck. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(21), 31-36.
https://hdl.handle.net/21.15107/rcub_farfar_306

Šavikin-Fodulović K, Stojanović D, Menković N, Krstić D, Janković T. Phytochemical and anatomic study of plant species Gentiana dinarica Beck. in Lekovite sirovine. 2001;(21):31-36.
https://hdl.handle.net/21.15107/rcub_farfar_306 .

Šavikin-Fodulović, Katarina, Stojanović, Danilo, Menković, Nebojša, Krstić, Dijana, Janković, Teodora, "Phytochemical and anatomic study of plant species Gentiana dinarica Beck" in Lekovite sirovine, no. 21 (2001):31-36,
https://hdl.handle.net/21.15107/rcub_farfar_306 .

TY  - JOUR
AU  - Šavikin-Fodulović, Katarina
AU  - Stojanović, Danilo
AU  - Menković, Nebojša
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/268
AB  - Iris reichenbachii Heuffel (Iridaceae) is an endemic plant species of Balkan penninsula and Romania. Pharmacognostic study of this species are not done till now. The microscopic preparations of the leaves were analyzed and specific orientation of vessels were noticed. Secondary metabolites were analyzed in the samples of leaves collected in different vegetative phase, flowers, fruits and rhizomes. In the samples, g-pyrones and secoiridoids were detected. Among γ-pyrones, a xanthone C-glycoside mangiferin were one of the most abundant compound. It's content varied from 0.5%-8.34% depending of plant organ or vegetative phase of development.
AB  - Vrsta Iris reichenbachii je endemit Balkanskog poluostrva i Rumunije. Do sada nije farmakognozijski izučavana. U ovom radu je ispitivana anatomska građa lista pri čemu je uočena specifična orijentacija provodnih snopića. Prisustvo sekundarnih metabolita ispitivano je u uzorcima listova, cvetova, plodova i rizoma. Detektovana su jedinjenja g-pironske strukture (ksantoni, flavonoidi) među kojima se po zastupljenosti u uzorcima, kao i po količini ističe ksanton mangiferin. Sadržaj mangiferina varira zavisno od faze razvića i biljnog organa (0.5%-8.34%). Detektovani su i sekoiridoidi od kojih se u značajnijoj količini pojavljuju svercijamarin i genciopikrin.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Phytochemical and anatomic study of plant species Iris reichenbachii Heuff.
T1  - Fitohemijska i anatomska analiza vrste Iris reichenbachii Heuff.
IS  - 20
SP  - 21
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_farfar_268
ER  - 

@article{
author = "Šavikin-Fodulović, Katarina and Stojanović, Danilo and Menković, Nebojša",
year = "2000",
abstract = "Iris reichenbachii Heuffel (Iridaceae) is an endemic plant species of Balkan penninsula and Romania. Pharmacognostic study of this species are not done till now. The microscopic preparations of the leaves were analyzed and specific orientation of vessels were noticed. Secondary metabolites were analyzed in the samples of leaves collected in different vegetative phase, flowers, fruits and rhizomes. In the samples, g-pyrones and secoiridoids were detected. Among γ-pyrones, a xanthone C-glycoside mangiferin were one of the most abundant compound. It's content varied from 0.5%-8.34% depending of plant organ or vegetative phase of development., Vrsta Iris reichenbachii je endemit Balkanskog poluostrva i Rumunije. Do sada nije farmakognozijski izučavana. U ovom radu je ispitivana anatomska građa lista pri čemu je uočena specifična orijentacija provodnih snopića. Prisustvo sekundarnih metabolita ispitivano je u uzorcima listova, cvetova, plodova i rizoma. Detektovana su jedinjenja g-pironske strukture (ksantoni, flavonoidi) među kojima se po zastupljenosti u uzorcima, kao i po količini ističe ksanton mangiferin. Sadržaj mangiferina varira zavisno od faze razvića i biljnog organa (0.5%-8.34%). Detektovani su i sekoiridoidi od kojih se u značajnijoj količini pojavljuju svercijamarin i genciopikrin.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Phytochemical and anatomic study of plant species Iris reichenbachii Heuff., Fitohemijska i anatomska analiza vrste Iris reichenbachii Heuff.",
number = "20",
pages = "21-26",
url = "https://hdl.handle.net/21.15107/rcub_farfar_268"
}

Šavikin-Fodulović, K., Stojanović, D.,& Menković, N.. (2000). Phytochemical and anatomic study of plant species Iris reichenbachii Heuff.. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(20), 21-26.
https://hdl.handle.net/21.15107/rcub_farfar_268

Šavikin-Fodulović K, Stojanović D, Menković N. Phytochemical and anatomic study of plant species Iris reichenbachii Heuff.. in Lekovite sirovine. 2000;(20):21-26.
https://hdl.handle.net/21.15107/rcub_farfar_268 .

Šavikin-Fodulović, Katarina, Stojanović, Danilo, Menković, Nebojša, "Phytochemical and anatomic study of plant species Iris reichenbachii Heuff." in Lekovite sirovine, no. 20 (2000):21-26,
https://hdl.handle.net/21.15107/rcub_farfar_268 .