TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Klisić, Aleksandra
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Memon, Lidija
AU  - Gaković, Branka
AU  - Bogavac-Stanojević, Nataša
AU  - Spasojević-Kalimanovska, Vesna
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4677
AB  - Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.
PB  - Termedia Publishing House Ltd.
T2  - Archives of Medical Science
T1  - Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
VL  - 19
IS  - 2
SP  - 313
EP  - 323
DO  - 10.5114/aoms/136074
ER  - 

@article{
author = "Vukašinović, Aleksandra and Ostanek, Barbara and Klisić, Aleksandra and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Memon, Lidija and Gaković, Branka and Bogavac-Stanojević, Nataša and Spasojević-Kalimanovska, Vesna and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.",
publisher = "Termedia Publishing House Ltd.",
journal = "Archives of Medical Science",
title = "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress",
volume = "19",
number = "2",
pages = "313-323",
doi = "10.5114/aoms/136074"
}

Vukašinović, A., Ostanek, B., Klisić, A., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Memon, L., Gaković, B., Bogavac-Stanojević, N., Spasojević-Kalimanovska, V., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science
Termedia Publishing House Ltd.., 19(2), 313-323.
https://doi.org/10.5114/aoms/136074

Vukašinović A, Ostanek B, Klisić A, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Memon L, Gaković B, Bogavac-Stanojević N, Spasojević-Kalimanovska V, Marc J, Nešković A, Kotur-Stevuljević J. Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science. 2023;19(2):313-323.
doi:10.5114/aoms/136074 .

Vukašinović, Aleksandra, Ostanek, Barbara, Klisić, Aleksandra, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Memon, Lidija, Gaković, Branka, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress" in Archives of Medical Science, 19, no. 2 (2023):313-323,
https://doi.org/10.5114/aoms/136074 . .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Klisic, Aleksandra
AU  - Ostanek, Barbara
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Bogavac-Stanojević, Nataša
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5078
AB  - In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?
VL  - 24
IS  - 18
DO  - 10.3390/ijms241814308
ER  - 

@article{
author = "Vukašinović, Aleksandra and Klisic, Aleksandra and Ostanek, Barbara and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Bogavac-Stanojević, Nataša and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?",
volume = "24",
number = "18",
doi = "10.3390/ijms241814308"
}

Vukašinović, A., Klisic, A., Ostanek, B., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Bogavac-Stanojević, N., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences
MDPI., 24(18).
https://doi.org/10.3390/ijms241814308

Vukašinović A, Klisic A, Ostanek B, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Bogavac-Stanojević N, Marc J, Nešković A, Kotur-Stevuljević J. Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences. 2023;24(18).
doi:10.3390/ijms241814308 .

Vukašinović, Aleksandra, Klisic, Aleksandra, Ostanek, Barbara, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Bogavac-Stanojević, Nataša, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?" in International Journal of Molecular Sciences, 24, no. 18 (2023),
https://doi.org/10.3390/ijms241814308 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Ninić, Ana
AU  - Ostanek, Barbara
AU  - Bojanin, Dragana
AU  - Milenković, Tatjana
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Vekić, Jelena
AU  - Marc, Janja
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4255
AB  - Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients.
AB  - Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus
T1  - Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om
VL  - 41
IS  - 3
SP  - 282
EP  - 289
DO  - 10.5937/jomb0-33220
ER  - 

@article{
author = "Sopić, Miron and Ninić, Ana and Ostanek, Barbara and Bojanin, Dragana and Milenković, Tatjana and Munjas, Jelena and Mihajlović, Marija and Vekić, Jelena and Marc, Janja and Spasojević-Kalimanovska, Vesna",
year = "2022",
abstract = "Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients., Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus, Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om",
volume = "41",
number = "3",
pages = "282-289",
doi = "10.5937/jomb0-33220"
}

Sopić, M., Ninić, A., Ostanek, B., Bojanin, D., Milenković, T., Munjas, J., Mihajlović, M., Vekić, J., Marc, J.,& Spasojević-Kalimanovska, V.. (2022). Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 41(3), 282-289.
https://doi.org/10.5937/jomb0-33220

Sopić M, Ninić A, Ostanek B, Bojanin D, Milenković T, Munjas J, Mihajlović M, Vekić J, Marc J, Spasojević-Kalimanovska V. Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry. 2022;41(3):282-289.
doi:10.5937/jomb0-33220 .

Sopić, Miron, Ninić, Ana, Ostanek, Barbara, Bojanin, Dragana, Milenković, Tatjana, Munjas, Jelena, Mihajlović, Marija, Vekić, Jelena, Marc, Janja, Spasojević-Kalimanovska, Vesna, "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus" in Journal of Medical Biochemistry, 41, no. 3 (2022):282-289,
https://doi.org/10.5937/jomb0-33220 . .

TY  - JOUR
AU  - Riad, Abanoub
AU  - Schünemann, Holger
AU  - Sameh, Attia
AU  - Poklepović Peričić, Tina
AU  - Franka Žuljević, Marija
AU  - Jürisson, Mikk
AU  - Kalda, Ruth
AU  - Lang, Katrin
AU  - Morankar, Sudhakar
AU  - Ali Yesuf, Elias
AU  - Mekhemar, Mohamed
AU  - Danso-Appiah, Anthony
AU  - Sofi-Mahmudi, Ahmad
AU  - Pérez-Gaxiola, Giordano
AU  - Dziedzic, Arkadiusz
AU  - Apóstolo, João
AU  - Cardoso, Daniela
AU  - Marc, Janja
AU  - Moreno-Casbas, Mayte
AU  - Shey Wiysonge, Charles
AU  - Qaseem, Amir
AU  - Gryschek, Anna
AU  - Tadić, Ivana
AU  - Hussain, Salman
AU  - Ahmed Khan, Mohammed
AU  - Klugarova, Jitka
AU  - Pokorna, Andrea
AU  - Koščík, Michal
AU  - Klugar, Miloslav
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4212
AB  - Background: Coronavirus disease (COVID-19) vaccine-related side effects have a determinant role in the public decision regarding vaccination. Therefore, this study has been designed to actively monitor the safety and effectiveness of COVID-19 vaccines globally.

Methods: A multi-country, three-phase study including a cross-sectional survey to test for the short-term side effects of COVID-19 vaccines among target population groups. In the second phase, we will monitor the booster doses' side effects, while in the third phase, the long-term safety and effectiveness will be investigated. A validated, self-administered questionnaire will be used to collect data from the target population; Results: The study protocol has been registered at ClinicalTrials.gov, with the identifier NCT04834869.

Conclusions: CoVaST is the first independent study aiming to monitor the side effects of COVID-19 vaccines following booster doses, and the long-term safety and effectiveness of said vaccines.
PB  - MDPI
T2  - International Journal of Environmental Research and Public Health
T1  - COVID-19 Vaccines Safety Tracking (CoVaST): Protocol of a Multi-Center Prospective Cohort Study for Active Surveillance of COVID-19 Vaccines’ Side Effects
VL  - 18
IS  - 15
SP  - 7859
DO  - 10.3390/ijerph18157859
ER  - 

@article{
author = "Riad, Abanoub and Schünemann, Holger and Sameh, Attia and Poklepović Peričić, Tina and Franka Žuljević, Marija and Jürisson, Mikk and Kalda, Ruth and Lang, Katrin and Morankar, Sudhakar and Ali Yesuf, Elias and Mekhemar, Mohamed and Danso-Appiah, Anthony and Sofi-Mahmudi, Ahmad and Pérez-Gaxiola, Giordano and Dziedzic, Arkadiusz and Apóstolo, João and Cardoso, Daniela and Marc, Janja and Moreno-Casbas, Mayte and Shey Wiysonge, Charles and Qaseem, Amir and Gryschek, Anna and Tadić, Ivana and Hussain, Salman and Ahmed Khan, Mohammed and Klugarova, Jitka and Pokorna, Andrea and Koščík, Michal and Klugar, Miloslav",
year = "2021",
abstract = "Background: Coronavirus disease (COVID-19) vaccine-related side effects have a determinant role in the public decision regarding vaccination. Therefore, this study has been designed to actively monitor the safety and effectiveness of COVID-19 vaccines globally.

Methods: A multi-country, three-phase study including a cross-sectional survey to test for the short-term side effects of COVID-19 vaccines among target population groups. In the second phase, we will monitor the booster doses' side effects, while in the third phase, the long-term safety and effectiveness will be investigated. A validated, self-administered questionnaire will be used to collect data from the target population; Results: The study protocol has been registered at ClinicalTrials.gov, with the identifier NCT04834869.

Conclusions: CoVaST is the first independent study aiming to monitor the side effects of COVID-19 vaccines following booster doses, and the long-term safety and effectiveness of said vaccines.",
publisher = "MDPI",
journal = "International Journal of Environmental Research and Public Health",
title = "COVID-19 Vaccines Safety Tracking (CoVaST): Protocol of a Multi-Center Prospective Cohort Study for Active Surveillance of COVID-19 Vaccines’ Side Effects",
volume = "18",
number = "15",
pages = "7859",
doi = "10.3390/ijerph18157859"
}

Riad, A., Schünemann, H., Sameh, A., Poklepović Peričić, T., Franka Žuljević, M., Jürisson, M., Kalda, R., Lang, K., Morankar, S., Ali Yesuf, E., Mekhemar, M., Danso-Appiah, A., Sofi-Mahmudi, A., Pérez-Gaxiola, G., Dziedzic, A., Apóstolo, J., Cardoso, D., Marc, J., Moreno-Casbas, M., Shey Wiysonge, C., Qaseem, A., Gryschek, A., Tadić, I., Hussain, S., Ahmed Khan, M., Klugarova, J., Pokorna, A., Koščík, M.,& Klugar, M.. (2021). COVID-19 Vaccines Safety Tracking (CoVaST): Protocol of a Multi-Center Prospective Cohort Study for Active Surveillance of COVID-19 Vaccines’ Side Effects. in International Journal of Environmental Research and Public Health
MDPI., 18(15), 7859.
https://doi.org/10.3390/ijerph18157859

Riad A, Schünemann H, Sameh A, Poklepović Peričić T, Franka Žuljević M, Jürisson M, Kalda R, Lang K, Morankar S, Ali Yesuf E, Mekhemar M, Danso-Appiah A, Sofi-Mahmudi A, Pérez-Gaxiola G, Dziedzic A, Apóstolo J, Cardoso D, Marc J, Moreno-Casbas M, Shey Wiysonge C, Qaseem A, Gryschek A, Tadić I, Hussain S, Ahmed Khan M, Klugarova J, Pokorna A, Koščík M, Klugar M. COVID-19 Vaccines Safety Tracking (CoVaST): Protocol of a Multi-Center Prospective Cohort Study for Active Surveillance of COVID-19 Vaccines’ Side Effects. in International Journal of Environmental Research and Public Health. 2021;18(15):7859.
doi:10.3390/ijerph18157859 .

Riad, Abanoub, Schünemann, Holger, Sameh, Attia, Poklepović Peričić, Tina, Franka Žuljević, Marija, Jürisson, Mikk, Kalda, Ruth, Lang, Katrin, Morankar, Sudhakar, Ali Yesuf, Elias, Mekhemar, Mohamed, Danso-Appiah, Anthony, Sofi-Mahmudi, Ahmad, Pérez-Gaxiola, Giordano, Dziedzic, Arkadiusz, Apóstolo, João, Cardoso, Daniela, Marc, Janja, Moreno-Casbas, Mayte, Shey Wiysonge, Charles, Qaseem, Amir, Gryschek, Anna, Tadić, Ivana, Hussain, Salman, Ahmed Khan, Mohammed, Klugarova, Jitka, Pokorna, Andrea, Koščík, Michal, Klugar, Miloslav, "COVID-19 Vaccines Safety Tracking (CoVaST): Protocol of a Multi-Center Prospective Cohort Study for Active Surveillance of COVID-19 Vaccines’ Side Effects" in International Journal of Environmental Research and Public Health, 18, no. 15 (2021):7859,
https://doi.org/10.3390/ijerph18157859 . .

TY  - JOUR
AU  - Rauner, Martina
AU  - Foessl, Ines
AU  - Formosa, Melissa
AU  - Kague, Erika
AU  - Prijatelj, Vid
AU  - Alonso Lopez, Nerea
AU  - Banerjee, Bodhisattwa
AU  - Bergen, Dylan
AU  - Busse, Björn
AU  - Calado, Angelo
AU  - Douni, Eleni
AU  - Gabet, Yankel
AU  - Garcı´a Giralt, Natalia
AU  - Grinberg, Daniel
AU  - Lovsin, Nika
AU  - Nogues Solan, Xavier
AU  - Ostanek, Barbara
AU  - Pavlos, Nathan
AU  - Rivadeneira, Fernando
AU  - Soldatović, Ivan
AU  - van de Peppel, Jeroen
AU  - van der Eerden, Bram
AU  - van Hul, Wim
AU  - Balcells, Susanna
AU  - Marc, Janja
AU  - Reppe, Sjur
AU  - Søe, Kent
AU  - Karasik, David
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4019
AB  - The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques
VL  - 12
DO  - 10.3389/fendo.2021.731217
ER  - 

@article{
author = "Rauner, Martina and Foessl, Ines and Formosa, Melissa and Kague, Erika and Prijatelj, Vid and Alonso Lopez, Nerea and Banerjee, Bodhisattwa and Bergen, Dylan and Busse, Björn and Calado, Angelo and Douni, Eleni and Gabet, Yankel and Garcı´a Giralt, Natalia and Grinberg, Daniel and Lovsin, Nika and Nogues Solan, Xavier and Ostanek, Barbara and Pavlos, Nathan and Rivadeneira, Fernando and Soldatović, Ivan and van de Peppel, Jeroen and van der Eerden, Bram and van Hul, Wim and Balcells, Susanna and Marc, Janja and Reppe, Sjur and Søe, Kent and Karasik, David",
year = "2021",
abstract = "The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques",
volume = "12",
doi = "10.3389/fendo.2021.731217"
}

Rauner, M., Foessl, I., Formosa, M., Kague, E., Prijatelj, V., Alonso Lopez, N., Banerjee, B., Bergen, D., Busse, B., Calado, A., Douni, E., Gabet, Y., Garcı´a Giralt, N., Grinberg, D., Lovsin, N., Nogues Solan, X., Ostanek, B., Pavlos, N., Rivadeneira, F., Soldatović, I., van de Peppel, J., van der Eerden, B., van Hul, W., Balcells, S., Marc, J., Reppe, S., Søe, K.,& Karasik, D.. (2021). Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology
Frontiers Media S.A.., 12.
https://doi.org/10.3389/fendo.2021.731217

Rauner M, Foessl I, Formosa M, Kague E, Prijatelj V, Alonso Lopez N, Banerjee B, Bergen D, Busse B, Calado A, Douni E, Gabet Y, Garcı´a Giralt N, Grinberg D, Lovsin N, Nogues Solan X, Ostanek B, Pavlos N, Rivadeneira F, Soldatović I, van de Peppel J, van der Eerden B, van Hul W, Balcells S, Marc J, Reppe S, Søe K, Karasik D. Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology. 2021;12.
doi:10.3389/fendo.2021.731217 .

Rauner, Martina, Foessl, Ines, Formosa, Melissa, Kague, Erika, Prijatelj, Vid, Alonso Lopez, Nerea, Banerjee, Bodhisattwa, Bergen, Dylan, Busse, Björn, Calado, Angelo, Douni, Eleni, Gabet, Yankel, Garcı´a Giralt, Natalia, Grinberg, Daniel, Lovsin, Nika, Nogues Solan, Xavier, Ostanek, Barbara, Pavlos, Nathan, Rivadeneira, Fernando, Soldatović, Ivan, van de Peppel, Jeroen, van der Eerden, Bram, van Hul, Wim, Balcells, Susanna, Marc, Janja, Reppe, Sjur, Søe, Kent, Karasik, David, "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques" in Frontiers in Endocrinology, 12 (2021),
https://doi.org/10.3389/fendo.2021.731217 . .

TY  - JOUR
AU  - Bogavac-Stanojević, Nataša
AU  - Kotur-Stevuljević, Jelena
AU  - Cerne, Darko
AU  - Zupan, Janja
AU  - Marc, Janja
AU  - Vujić, Zorica
AU  - Crevar-Sakač, Milkica
AU  - Sopić, Miron
AU  - Munjas, Jelena
AU  - Radenković, Miroslav
AU  - Jelić-Ivanović, Zorana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3189
AB  - Context: Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect cells against oxidative damage. Objectives: We examined ALT effects on deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)]. Materials and methods: Eighteen male Wistar albino rats were divided into three groups (n=6/group): The control group (CG) was fed with standard pellet chow for 11 weeks; the AD group was fed for a similar period of time with pellet chow supplemented with 2% cholesterol, 3% sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 1 week), the atherogenic diet (see above) for the following 4 weeks and then with ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, isoquercitrin and rutin. Results: Normalized HO-1 [0.11 (0.04-0.24)] and MCP-1 [0.29 (0.21-0.47)] mRNA levels and DNA scores [12.50 (4.50-36.50)] were significantly lower in the ADA group than in the AD group [0.84 (0.35-2.51)], p = 0.021 for HO-1 [0.85 (0.61-3.45)], p = 0.047 for MCP-1 and [176.5 (66.50-221.25)], p = 0.020 for DNA scores. HO-1 mRNA was lower in the ADA group than in the CG group [0.30 (0.21-0.71), p = 0.049]. Conclusions: Supplementation with ALT limited the effects of the atherogenic diet through reduced MCP-1 expression, thereby preventing oxidative damage.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Biology
T1  - The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet
VL  - 56
IS  - 1
SP  - 138
EP  - 144
DO  - 10.1080/13880209.2018.1434549
ER  - 

@article{
author = "Bogavac-Stanojević, Nataša and Kotur-Stevuljević, Jelena and Cerne, Darko and Zupan, Janja and Marc, Janja and Vujić, Zorica and Crevar-Sakač, Milkica and Sopić, Miron and Munjas, Jelena and Radenković, Miroslav and Jelić-Ivanović, Zorana",
year = "2018",
abstract = "Context: Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect cells against oxidative damage. Objectives: We examined ALT effects on deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)]. Materials and methods: Eighteen male Wistar albino rats were divided into three groups (n=6/group): The control group (CG) was fed with standard pellet chow for 11 weeks; the AD group was fed for a similar period of time with pellet chow supplemented with 2% cholesterol, 3% sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 1 week), the atherogenic diet (see above) for the following 4 weeks and then with ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, isoquercitrin and rutin. Results: Normalized HO-1 [0.11 (0.04-0.24)] and MCP-1 [0.29 (0.21-0.47)] mRNA levels and DNA scores [12.50 (4.50-36.50)] were significantly lower in the ADA group than in the AD group [0.84 (0.35-2.51)], p = 0.021 for HO-1 [0.85 (0.61-3.45)], p = 0.047 for MCP-1 and [176.5 (66.50-221.25)], p = 0.020 for DNA scores. HO-1 mRNA was lower in the ADA group than in the CG group [0.30 (0.21-0.71), p = 0.049]. Conclusions: Supplementation with ALT limited the effects of the atherogenic diet through reduced MCP-1 expression, thereby preventing oxidative damage.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Biology",
title = "The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet",
volume = "56",
number = "1",
pages = "138-144",
doi = "10.1080/13880209.2018.1434549"
}

Bogavac-Stanojević, N., Kotur-Stevuljević, J., Cerne, D., Zupan, J., Marc, J., Vujić, Z., Crevar-Sakač, M., Sopić, M., Munjas, J., Radenković, M.,& Jelić-Ivanović, Z.. (2018). The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet. in Pharmaceutical Biology
Taylor & Francis Ltd, Abingdon., 56(1), 138-144.
https://doi.org/10.1080/13880209.2018.1434549

Bogavac-Stanojević N, Kotur-Stevuljević J, Cerne D, Zupan J, Marc J, Vujić Z, Crevar-Sakač M, Sopić M, Munjas J, Radenković M, Jelić-Ivanović Z. The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet. in Pharmaceutical Biology. 2018;56(1):138-144.
doi:10.1080/13880209.2018.1434549 .

Bogavac-Stanojević, Nataša, Kotur-Stevuljević, Jelena, Cerne, Darko, Zupan, Janja, Marc, Janja, Vujić, Zorica, Crevar-Sakač, Milkica, Sopić, Miron, Munjas, Jelena, Radenković, Miroslav, Jelić-Ivanović, Zorana, "The role of artichoke leaf tincture (Cynara scolymus) in the suppression of DNA damage and atherosclerosis in rats fed an atherogenic diet" in Pharmaceutical Biology, 56, no. 1 (2018):138-144,
https://doi.org/10.1080/13880209.2018.1434549 . .

TY  - JOUR
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Simić-Ogrizović, Sanja
AU  - Vekić, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Cerne, Darko
AU  - Kocbek, Petra
AU  - Marc, Janja
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3085
AB  - Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant-antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score (P  lt  .001), oxy score (P  lt  .01), inflammation score (P  lt  .001), and the DOI score (P  lt  .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP (P  lt  .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction (P  lt  .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (rho = 0.510, P  lt  .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Angiology
T1  - Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease
VL  - 69
IS  - 10
SP  - 861
EP  - 870
DO  - 10.1177/0003319718780041
ER  - 

@article{
author = "Miljković, Milica and Stefanović, Aleksandra and Simić-Ogrizović, Sanja and Vekić, Jelena and Bogavac-Stanojević, Nataša and Cerne, Darko and Kocbek, Petra and Marc, Janja and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena",
year = "2018",
abstract = "Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant-antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score (P  lt  .001), oxy score (P  lt  .01), inflammation score (P  lt  .001), and the DOI score (P  lt  .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP (P  lt  .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction (P  lt  .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (rho = 0.510, P  lt  .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Angiology",
title = "Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease",
volume = "69",
number = "10",
pages = "861-870",
doi = "10.1177/0003319718780041"
}

Miljković, M., Stefanović, A., Simić-Ogrizović, S., Vekić, J., Bogavac-Stanojević, N., Cerne, D., Kocbek, P., Marc, J., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V.,& Kotur-Stevuljević, J.. (2018). Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease. in Angiology
Sage Publications Inc, Thousand Oaks., 69(10), 861-870.
https://doi.org/10.1177/0003319718780041

Miljković M, Stefanović A, Simić-Ogrizović S, Vekić J, Bogavac-Stanojević N, Cerne D, Kocbek P, Marc J, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Kotur-Stevuljević J. Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease. in Angiology. 2018;69(10):861-870.
doi:10.1177/0003319718780041 .

Miljković, Milica, Stefanović, Aleksandra, Simić-Ogrizović, Sanja, Vekić, Jelena, Bogavac-Stanojević, Nataša, Cerne, Darko, Kocbek, Petra, Marc, Janja, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, "Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease" in Angiology, 69, no. 10 (2018):861-870,
https://doi.org/10.1177/0003319718780041 . .

TY  - JOUR
AU  - Mirjanić-Azarić, Bosa
AU  - Jelić-Ivanović, Zorana
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Juergens, Guenther
AU  - Milivojac, Tatjana
AU  - Avram, Sanja
AU  - Ćorić, Jozo
AU  - Marc, Janja
AU  - Černe, Darko
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2432
AB  - Background: High-density lipoproteins (HDL) have atheroprotective biological properties: antioxidative, anti-apoptotic, anti-inflammatory, and they have the efflux capacity of cellular cholesterol. Plasma mRNA analysis can be used to investigate statin pleiotropy in vivo as a new analytical tool for non-invasive assessment of gene expression in vascular beds. The aim of this study was to assess the pleiotropic effects of atorvastatin in stable angina patients with highrisk values (group A) as compared with patients who had borderline and desirable HDL-cholesterol (HDL-C) values (group B). Methods: The atorvastatin therapy (20 mg/day) was given to forty-three patients with stable angina for 10 weeks. We investigated three statin pleiotropy-targeted genes: intercellular adhesion molecule-1, chemokine (C-C motif) ligand 2 and cathepsin S and assessed by gel electrophoresis gradient the effects of atorvastatin on HDL size and subclasses. Results: In group A, after therapy, HDL-C concentration was significantly increased but not in group B. Atorvastatin lowered plasma chemokine (C-C motif) ligand 2 and intercellular adhesion molecule-1 mRNA levels in both groups, but did not change the plasma cathepsin S mRNA levels. In group A only, baseline total bilirubin showed negative cor relations with the genes of cathepsin S (r=-0.506; p = 0.023) and significantly increased after therapy. Conclusions: HDL-C and bilirubin can be promising therapeutic targets in the treatment of cardiovascular diseases. Analysis of cell-free mRNA in plasma might become a useful tool for estimating statin pleiotropy.
AB  - Uvod: Lipoproteini velike gustine (HDL) imaju ateroprotektivne biološke osobine: antioksidativne, antiapoptotičke, antiinflamatorne kao i kapacitet da izvlače holesterol iz ćelija. Analiza plazmatske mRNA može da se koristi za ispitivanje plejotropnih efekata statina in vivo kao novo analitičko sredstvo za neinvazivnu procenu ekspresije gena u zidu krvnog suda. Cilj ove studije je bio da se procene plejotropni efekti atorvastatina kod pacijenata sa stabilnom anginom sa visokorizičnim vrednostima (grupa A) u odnosu na pacijente sa graničnim i poželjnim vrednostima HDL holesterola (HDLC) (grupa B). Metode: Četrdeset tri pacijenta sa stabilnom anginom su primala terapiju atorvastatinom (20 mg/dan) 10 nedelja. Mi smo ispitivali tri gena značajna za plejotropno delovanje statina: intracelularni adhezioni molekul-1, hemokin (C-C motiv) ligand 2 i katepsin S i procenjivali smo efekte atorvastatina na veličinu i raspodelu HDL subfrakcija pomoću elektroforeze na poliakrilamidnom gradijent gelu. Rezultati: U grupi A, posle terapije, HDL-C koncentracija se značajno povećala, ali ne i u grupi B. Atorvastatin je snizio plazmatski nivo hemokin (C-C motiv) liganda 2 i intracelularnog adhezionog molekula-1 mRNA u obe grupe, ali nije promenio plazmatski nivo gena za katepsin S. Samo u grupi A, ukupni bilirubin je pokazao negativnu korelaciju sa genom za katepsin S (r = -0,506; p = 0,023) pre započinjanja terapije i značajni porast nakon terapije atorvastatinom. Zaključak: HDL-C i bilirubin mogu biti obećavajući terapijski ciljevi u lečenju kardiovaskularnih bolesti. Analiza slobodne mRNA (eng. cell-free mRNA) u plazmi može postati korisno sredstvo za procenu plejotropnog delovanja statina.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - The Pleiotropic Effects of Atorvastatin on Stable Angina Patients: Evidence by Analysis of High-Density Lipoprotein Size and Subclasses, and Plasma mRNA
T1  - Plejotropni efekti atorvastatina kod pacijenata sa stabilnom anginom - dokazi dobijeni analizom veličine i raspodele subfrakcija lipoproteina velike gustine i plazmatske mRNA
VL  - 34
IS  - 3
SP  - 314
EP  - 322
DO  - 10.2478/jomb-2014-0058
ER  - 

@article{
author = "Mirjanić-Azarić, Bosa and Jelić-Ivanović, Zorana and Zeljković, Aleksandra and Vekić, Jelena and Juergens, Guenther and Milivojac, Tatjana and Avram, Sanja and Ćorić, Jozo and Marc, Janja and Černe, Darko",
year = "2015",
abstract = "Background: High-density lipoproteins (HDL) have atheroprotective biological properties: antioxidative, anti-apoptotic, anti-inflammatory, and they have the efflux capacity of cellular cholesterol. Plasma mRNA analysis can be used to investigate statin pleiotropy in vivo as a new analytical tool for non-invasive assessment of gene expression in vascular beds. The aim of this study was to assess the pleiotropic effects of atorvastatin in stable angina patients with highrisk values (group A) as compared with patients who had borderline and desirable HDL-cholesterol (HDL-C) values (group B). Methods: The atorvastatin therapy (20 mg/day) was given to forty-three patients with stable angina for 10 weeks. We investigated three statin pleiotropy-targeted genes: intercellular adhesion molecule-1, chemokine (C-C motif) ligand 2 and cathepsin S and assessed by gel electrophoresis gradient the effects of atorvastatin on HDL size and subclasses. Results: In group A, after therapy, HDL-C concentration was significantly increased but not in group B. Atorvastatin lowered plasma chemokine (C-C motif) ligand 2 and intercellular adhesion molecule-1 mRNA levels in both groups, but did not change the plasma cathepsin S mRNA levels. In group A only, baseline total bilirubin showed negative cor relations with the genes of cathepsin S (r=-0.506; p = 0.023) and significantly increased after therapy. Conclusions: HDL-C and bilirubin can be promising therapeutic targets in the treatment of cardiovascular diseases. Analysis of cell-free mRNA in plasma might become a useful tool for estimating statin pleiotropy., Uvod: Lipoproteini velike gustine (HDL) imaju ateroprotektivne biološke osobine: antioksidativne, antiapoptotičke, antiinflamatorne kao i kapacitet da izvlače holesterol iz ćelija. Analiza plazmatske mRNA može da se koristi za ispitivanje plejotropnih efekata statina in vivo kao novo analitičko sredstvo za neinvazivnu procenu ekspresije gena u zidu krvnog suda. Cilj ove studije je bio da se procene plejotropni efekti atorvastatina kod pacijenata sa stabilnom anginom sa visokorizičnim vrednostima (grupa A) u odnosu na pacijente sa graničnim i poželjnim vrednostima HDL holesterola (HDLC) (grupa B). Metode: Četrdeset tri pacijenta sa stabilnom anginom su primala terapiju atorvastatinom (20 mg/dan) 10 nedelja. Mi smo ispitivali tri gena značajna za plejotropno delovanje statina: intracelularni adhezioni molekul-1, hemokin (C-C motiv) ligand 2 i katepsin S i procenjivali smo efekte atorvastatina na veličinu i raspodelu HDL subfrakcija pomoću elektroforeze na poliakrilamidnom gradijent gelu. Rezultati: U grupi A, posle terapije, HDL-C koncentracija se značajno povećala, ali ne i u grupi B. Atorvastatin je snizio plazmatski nivo hemokin (C-C motiv) liganda 2 i intracelularnog adhezionog molekula-1 mRNA u obe grupe, ali nije promenio plazmatski nivo gena za katepsin S. Samo u grupi A, ukupni bilirubin je pokazao negativnu korelaciju sa genom za katepsin S (r = -0,506; p = 0,023) pre započinjanja terapije i značajni porast nakon terapije atorvastatinom. Zaključak: HDL-C i bilirubin mogu biti obećavajući terapijski ciljevi u lečenju kardiovaskularnih bolesti. Analiza slobodne mRNA (eng. cell-free mRNA) u plazmi može postati korisno sredstvo za procenu plejotropnog delovanja statina.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "The Pleiotropic Effects of Atorvastatin on Stable Angina Patients: Evidence by Analysis of High-Density Lipoprotein Size and Subclasses, and Plasma mRNA, Plejotropni efekti atorvastatina kod pacijenata sa stabilnom anginom - dokazi dobijeni analizom veličine i raspodele subfrakcija lipoproteina velike gustine i plazmatske mRNA",
volume = "34",
number = "3",
pages = "314-322",
doi = "10.2478/jomb-2014-0058"
}

Mirjanić-Azarić, B., Jelić-Ivanović, Z., Zeljković, A., Vekić, J., Juergens, G., Milivojac, T., Avram, S., Ćorić, J., Marc, J.,& Černe, D.. (2015). The Pleiotropic Effects of Atorvastatin on Stable Angina Patients: Evidence by Analysis of High-Density Lipoprotein Size and Subclasses, and Plasma mRNA. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 34(3), 314-322.
https://doi.org/10.2478/jomb-2014-0058

Mirjanić-Azarić B, Jelić-Ivanović Z, Zeljković A, Vekić J, Juergens G, Milivojac T, Avram S, Ćorić J, Marc J, Černe D. The Pleiotropic Effects of Atorvastatin on Stable Angina Patients: Evidence by Analysis of High-Density Lipoprotein Size and Subclasses, and Plasma mRNA. in Journal of Medical Biochemistry. 2015;34(3):314-322.
doi:10.2478/jomb-2014-0058 .

Mirjanić-Azarić, Bosa, Jelić-Ivanović, Zorana, Zeljković, Aleksandra, Vekić, Jelena, Juergens, Guenther, Milivojac, Tatjana, Avram, Sanja, Ćorić, Jozo, Marc, Janja, Černe, Darko, "The Pleiotropic Effects of Atorvastatin on Stable Angina Patients: Evidence by Analysis of High-Density Lipoprotein Size and Subclasses, and Plasma mRNA" in Journal of Medical Biochemistry, 34, no. 3 (2015):314-322,
https://doi.org/10.2478/jomb-2014-0058 . .

TY  - JOUR
AU  - Mirjanić-Azarić, Bosa
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Đerić, Mirjana
AU  - Milivojac, Tatjana
AU  - Fonović, Ursa Pecar
AU  - Marc, Janja
AU  - Kos, Janko
AU  - Cerne, Darko
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2069
AB  - Aim: We hypothesized that, in stable angina patients, atorvastatin therapy lowers the cathepsin S (CTSS) concentrations, as assessed non-invasively according to a plasma analysis. In addition, the low-density lipoprotein (LDL) and high-density lipoprotein (HDL) size and subclasses in the plasma were analysed to establish the association between CTSS and lipoprotein metabolism and determine whether this association is atorvastatin-sensitive. Methods: A total of 43 patients with stable angina received atorvastatin therapy (20 mg/day, 10 weeks). The plasma CTSS mRNA levels, CTSS protein concentrations and CTSS activity, as well as LDL and HDL size and subclasses, were analysed before and after treatment. Results: Atorvastatin treatment did not change the plasma CTSS mRNA levels, although it lowered the plasma CTSS concentrations and activity. An increased plasma CTSS concentration and activity were found to be associated with a more atherogenic LDL subclass profile (a decreased dominant LDL size and increased percentage of small, dense LDL particles). The atorvastatin-induced CTSS-lowering effect was concomitant with an improvement in the LDL subclass profile, and the changes were found to be interrelated. Concomitant, interrelated changes in the CTSS levels and LDL subclass profiles were found in the LDL phenotype B patients only (a dominant LDL diameter of  lt = 25.5 nm at the start of the study). In this subgroup, lowering of the plasma CTSS mRNA level also correlated with lowering of the proportion of small, dense LDL particles. Conclusions: Atorvastatin-induced CTSS-lowering and LDL subclass profile improvements in the plasma of LDL phenotype B patients with stable angina are concomitant and interrelated.
PB  - Japan Atherosclerosis Soc, Tokyo
T2  - Journal of Atherosclerosis and Thrombosis
T1  - Interrelated Cathepsin S-Lowering and LDL Subclass Profile Improvements Induced by Atorvastatin in the Plasma of Stable Angina Patients
VL  - 21
IS  - 8
SP  - 868
EP  - 877
DO  - 10.5551/jat.21410
ER  - 

@article{
author = "Mirjanić-Azarić, Bosa and Vekić, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Đerić, Mirjana and Milivojac, Tatjana and Fonović, Ursa Pecar and Marc, Janja and Kos, Janko and Cerne, Darko",
year = "2014",
abstract = "Aim: We hypothesized that, in stable angina patients, atorvastatin therapy lowers the cathepsin S (CTSS) concentrations, as assessed non-invasively according to a plasma analysis. In addition, the low-density lipoprotein (LDL) and high-density lipoprotein (HDL) size and subclasses in the plasma were analysed to establish the association between CTSS and lipoprotein metabolism and determine whether this association is atorvastatin-sensitive. Methods: A total of 43 patients with stable angina received atorvastatin therapy (20 mg/day, 10 weeks). The plasma CTSS mRNA levels, CTSS protein concentrations and CTSS activity, as well as LDL and HDL size and subclasses, were analysed before and after treatment. Results: Atorvastatin treatment did not change the plasma CTSS mRNA levels, although it lowered the plasma CTSS concentrations and activity. An increased plasma CTSS concentration and activity were found to be associated with a more atherogenic LDL subclass profile (a decreased dominant LDL size and increased percentage of small, dense LDL particles). The atorvastatin-induced CTSS-lowering effect was concomitant with an improvement in the LDL subclass profile, and the changes were found to be interrelated. Concomitant, interrelated changes in the CTSS levels and LDL subclass profiles were found in the LDL phenotype B patients only (a dominant LDL diameter of  lt = 25.5 nm at the start of the study). In this subgroup, lowering of the plasma CTSS mRNA level also correlated with lowering of the proportion of small, dense LDL particles. Conclusions: Atorvastatin-induced CTSS-lowering and LDL subclass profile improvements in the plasma of LDL phenotype B patients with stable angina are concomitant and interrelated.",
publisher = "Japan Atherosclerosis Soc, Tokyo",
journal = "Journal of Atherosclerosis and Thrombosis",
title = "Interrelated Cathepsin S-Lowering and LDL Subclass Profile Improvements Induced by Atorvastatin in the Plasma of Stable Angina Patients",
volume = "21",
number = "8",
pages = "868-877",
doi = "10.5551/jat.21410"
}

Mirjanić-Azarić, B., Vekić, J., Zeljković, A., Jelić-Ivanović, Z., Đerić, M., Milivojac, T., Fonović, U. P., Marc, J., Kos, J.,& Cerne, D.. (2014). Interrelated Cathepsin S-Lowering and LDL Subclass Profile Improvements Induced by Atorvastatin in the Plasma of Stable Angina Patients. in Journal of Atherosclerosis and Thrombosis
Japan Atherosclerosis Soc, Tokyo., 21(8), 868-877.
https://doi.org/10.5551/jat.21410

Mirjanić-Azarić B, Vekić J, Zeljković A, Jelić-Ivanović Z, Đerić M, Milivojac T, Fonović UP, Marc J, Kos J, Cerne D. Interrelated Cathepsin S-Lowering and LDL Subclass Profile Improvements Induced by Atorvastatin in the Plasma of Stable Angina Patients. in Journal of Atherosclerosis and Thrombosis. 2014;21(8):868-877.
doi:10.5551/jat.21410 .

Mirjanić-Azarić, Bosa, Vekić, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Đerić, Mirjana, Milivojac, Tatjana, Fonović, Ursa Pecar, Marc, Janja, Kos, Janko, Cerne, Darko, "Interrelated Cathepsin S-Lowering and LDL Subclass Profile Improvements Induced by Atorvastatin in the Plasma of Stable Angina Patients" in Journal of Atherosclerosis and Thrombosis, 21, no. 8 (2014):868-877,
https://doi.org/10.5551/jat.21410 . .