TY  - JOUR
AU  - Banjac, Gorica
AU  - Ardalić, Daniela
AU  - Cabunac, Petar
AU  - Ivanišević, Jasmina
AU  - Vekić, Jelena
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4659
AB  - Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role.
AB  - Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.
PB  - Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet
T2  - Vojnosanitetski pregled
T1  - Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia
T1  - Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji
VL  - 80
IS  - 2
SP  - 121
EP  - 127
DO  - 10.2298/VSP210413010B
ER  - 

@article{
author = "Banjac, Gorica and Ardalić, Daniela and Cabunac, Petar and Ivanišević, Jasmina and Vekić, Jelena and Miković, Željko and Stefanović, Aleksandra",
year = "2023",
abstract = "Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role., Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.",
publisher = "Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet",
journal = "Vojnosanitetski pregled",
title = "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia, Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji",
volume = "80",
number = "2",
pages = "121-127",
doi = "10.2298/VSP210413010B"
}

Banjac, G., Ardalić, D., Cabunac, P., Ivanišević, J., Vekić, J., Miković, Ž.,& Stefanović, A.. (2023). Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled
Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet., 80(2), 121-127.
https://doi.org/10.2298/VSP210413010B

Banjac G, Ardalić D, Cabunac P, Ivanišević J, Vekić J, Miković Ž, Stefanović A. Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled. 2023;80(2):121-127.
doi:10.2298/VSP210413010B .

Banjac, Gorica, Ardalić, Daniela, Cabunac, Petar, Ivanišević, Jasmina, Vekić, Jelena, Miković, Željko, Stefanović, Aleksandra, "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia" in Vojnosanitetski pregled, 80, no. 2 (2023):121-127,
https://doi.org/10.2298/VSP210413010B . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Ardalić, Daniela
AU  - Vekić, Jelena
AU  - Antonić, Tamara
AU  - Vladimirov, Sandra
AU  - Rizzo, Manfredi
AU  - Gojković, Tamara
AU  - Ivanišević, Jasmina
AU  - Mihajlović, Marija
AU  - Vujčić, Sanja
AU  - Cabunac, Petar
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4314
AB  - Metabolic disorders in pregnancy, particularly gestational diabetes mellitus (GDM), are associated with an increased risk for adverse pregnancy outcome and long-term cardiometabolic health of mother and child. This study analyzed changes of serum cholesterol synthesis and absorption markers during the course of high-risk pregnancies, with respect to the development of GDM. Possible associations of maternal lipid biomarkers with neonatal characteristics were also investigated. The study included 63 women with high risk for development of pregnancy complications. Size and proportions of small low-density (LDL) and high-density lipoprotein (HDL) particles were assessed across trimesters (T1–T3), as well as concentrations of cholesterol synthesis (lathosterol, desmosterol) and absorption markers (campesterol, β-sitosterol). During the study, 15 women developed GDM, while 48 had no complications (non-GDM). As compared to the non-GDM group, women with GDM had significantly higher triglycerides in each trimester, while having a lower HDL-C level in T3. In addition, they had significantly lower levels of β-sitosterol in T3 (p < 0.05). Cholesterol synthesis markers increased across trimesters in both groups. A decrease in serum β-sitosterol levels during the course of pregnancies affected by GDM was observed. The prevalence of small-sized HDL decreased in non-GDM, while in the GDM group remained unchanged across trimesters. Newborn’s size in the non-GDM group was significantly higher (p < 0.01) and inversely associated with proportions of both small, dense LDL and HDL particles (p < 0.05) in maternal plasma in T1. In conclusion, high-risk pregnancies affected by GDM are characterized by altered cholesterol absorption and HDL maturation. Advanced lipid testing may indicate disturbed lipid homeostasis in GDM.
PB  - MDPI
T2  - Metabolites
T1  - Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome
VL  - 12
IS  - 10
DO  - 10.3390/metabo12100959
ER  - 

@article{
author = "Zeljković, Aleksandra and Ardalić, Daniela and Vekić, Jelena and Antonić, Tamara and Vladimirov, Sandra and Rizzo, Manfredi and Gojković, Tamara and Ivanišević, Jasmina and Mihajlović, Marija and Vujčić, Sanja and Cabunac, Petar and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2022",
abstract = "Metabolic disorders in pregnancy, particularly gestational diabetes mellitus (GDM), are associated with an increased risk for adverse pregnancy outcome and long-term cardiometabolic health of mother and child. This study analyzed changes of serum cholesterol synthesis and absorption markers during the course of high-risk pregnancies, with respect to the development of GDM. Possible associations of maternal lipid biomarkers with neonatal characteristics were also investigated. The study included 63 women with high risk for development of pregnancy complications. Size and proportions of small low-density (LDL) and high-density lipoprotein (HDL) particles were assessed across trimesters (T1–T3), as well as concentrations of cholesterol synthesis (lathosterol, desmosterol) and absorption markers (campesterol, β-sitosterol). During the study, 15 women developed GDM, while 48 had no complications (non-GDM). As compared to the non-GDM group, women with GDM had significantly higher triglycerides in each trimester, while having a lower HDL-C level in T3. In addition, they had significantly lower levels of β-sitosterol in T3 (p < 0.05). Cholesterol synthesis markers increased across trimesters in both groups. A decrease in serum β-sitosterol levels during the course of pregnancies affected by GDM was observed. The prevalence of small-sized HDL decreased in non-GDM, while in the GDM group remained unchanged across trimesters. Newborn’s size in the non-GDM group was significantly higher (p < 0.01) and inversely associated with proportions of both small, dense LDL and HDL particles (p < 0.05) in maternal plasma in T1. In conclusion, high-risk pregnancies affected by GDM are characterized by altered cholesterol absorption and HDL maturation. Advanced lipid testing may indicate disturbed lipid homeostasis in GDM.",
publisher = "MDPI",
journal = "Metabolites",
title = "Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome",
volume = "12",
number = "10",
doi = "10.3390/metabo12100959"
}

Zeljković, A., Ardalić, D., Vekić, J., Antonić, T., Vladimirov, S., Rizzo, M., Gojković, T., Ivanišević, J., Mihajlović, M., Vujčić, S., Cabunac, P., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2022). Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome. in Metabolites
MDPI., 12(10).
https://doi.org/10.3390/metabo12100959

Zeljković A, Ardalić D, Vekić J, Antonić T, Vladimirov S, Rizzo M, Gojković T, Ivanišević J, Mihajlović M, Vujčić S, Cabunac P, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome. in Metabolites. 2022;12(10).
doi:10.3390/metabo12100959 .

Zeljković, Aleksandra, Ardalić, Daniela, Vekić, Jelena, Antonić, Tamara, Vladimirov, Sandra, Rizzo, Manfredi, Gojković, Tamara, Ivanišević, Jasmina, Mihajlović, Marija, Vujčić, Sanja, Cabunac, Petar, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome" in Metabolites, 12, no. 10 (2022),
https://doi.org/10.3390/metabo12100959 . .

TY  - CONF
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Marković, Gorica
AU  - Cabunac, Petar
AU  - Mihajlović, Marija
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4644
AB  - Despite significant progress in improving pregnancy outcomes in recent decades,
predicting the risk and treatment of preeclampsia are still major challenges in clinical
practice (1). The aim of this study was to examine non-routine biomarkers in preeclampsia
risk assessment. The study involved 90 women with high-risk pregnancies, 20 of whom
developed preeclampsia by the end of pregnancy. Biochemical parameters were determined
between the 12th and 13 th weeks of gestation. The results of the study showed that women
who later developed preeclampsia had higher concentrations of lathosterol, cholesterol
synthesis marker (p <0.05), inflammatory proteins - monocyte chemoattractant protein-1
(MCP-1), and resistin (p <0.01, both), as well as paraoxonase-1 (PON1) activity (p <0.05).
Binary logistic regression analysis showed that higher concentrations of lathosterol, MCP-1,
resistin, and PON-1 were associated with preeclampsia development. To determine whether
the parameters significant in univariate analysis, are independent predictors of
preeclampsia, we applied multivariate regression analysis. Clinical markers commonly used
in risk assessment (maternal age and body mass index, mean arterial pressure, and uterine
blood flow), lathosterol, MCP-1, resistin, and PON-1 were included in the model. MCP-1 and
resistin stood out as significant independent predictors of preeclampsia. The diagnostic
accuracy of the investigated model was excellent (AUC=0.859). The study results indicated
the importance of a multi-marker approach in risk assessment for preeclampsia
development.
AB  - Uprkos značajnom napretku u poboljšanju ishoda trudnoće poslednjih decenija,
predviđanje rizika i terapija preeklampsije su još uvijek veliki izazovi u kliničkoj praksi (1).
Cilj ove studije je bio ispitivanje biomarkera koji se ne koriste u rutinskoj praksi u proceni
rizika za razvoj preeklampsije. U studiji je učestvovalo 90 žena sa visokorizičnim
trudnoćama, od kojih je 20 razvilo preeklampsiju do kraja trudnoće. Biohemijski parametri
su određivani između 12. i 13. nedelje gestacije. Rezultati studije su pokazali da su žene koje
su razvile preeklampsiju imale više koncentracije latosterola, markera sinteze holesterola (p
<0,05), inflamatornih proteina - monocitnog hemoatraktantnog proteina-1 (MCP-1) i
rezistina (p < 0,01, oba), kao i aktivnost enzima paraoksonaze-1 (PON1) (p <0,05). Binarna
logistička regresiona analiza je pokazala da su više koncentracije latosterola, MCP-1,
rezistina i PON-1 povezane sa razvojem preeklampsije. Da bi se utvrdilo da su parametri koji
su se u univarijantnoj analizi pokazali značajnim, nezavisni prediktori preeklampsije,
primjenili smo multivarijantnu regresionu analizu. U model su ušli klinički parametri koji se
uobičajeno koriste u procjeni rizika (starost i indeks tjelesne mase majke, srednji arterijski
pritisak i protok krvi kroz matericu), latosterol, MCP-1, rezistin i PON-1. MCP-1 i rezistin su
se istakli kao značajni nezavisni prediktori preeklampsije. Pokazana je odlična dijagnostička
tačnost ispitivanog modela (AUC=0,859). Rezultati ove studije su ukazali na značaj
multimarkerskog pristupa u procjeni rizika za razvoj preeklampsije.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Novel biomarkers in preeclampsia risk assessment
T1  - Novi biomarkeri u procjeni rizika za razvoj preeklampsije
VL  - 72
IS  - 4 suplement
SP  - S620
EP  - S621
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4644
ER  - 

@conference{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Marković, Gorica and Cabunac, Petar and Mihajlović, Marija and Miković, Željko and Stefanović, Aleksandra",
year = "2022",
abstract = "Despite significant progress in improving pregnancy outcomes in recent decades,
predicting the risk and treatment of preeclampsia are still major challenges in clinical
practice (1). The aim of this study was to examine non-routine biomarkers in preeclampsia
risk assessment. The study involved 90 women with high-risk pregnancies, 20 of whom
developed preeclampsia by the end of pregnancy. Biochemical parameters were determined
between the 12th and 13 th weeks of gestation. The results of the study showed that women
who later developed preeclampsia had higher concentrations of lathosterol, cholesterol
synthesis marker (p <0.05), inflammatory proteins - monocyte chemoattractant protein-1
(MCP-1), and resistin (p <0.01, both), as well as paraoxonase-1 (PON1) activity (p <0.05).
Binary logistic regression analysis showed that higher concentrations of lathosterol, MCP-1,
resistin, and PON-1 were associated with preeclampsia development. To determine whether
the parameters significant in univariate analysis, are independent predictors of
preeclampsia, we applied multivariate regression analysis. Clinical markers commonly used
in risk assessment (maternal age and body mass index, mean arterial pressure, and uterine
blood flow), lathosterol, MCP-1, resistin, and PON-1 were included in the model. MCP-1 and
resistin stood out as significant independent predictors of preeclampsia. The diagnostic
accuracy of the investigated model was excellent (AUC=0.859). The study results indicated
the importance of a multi-marker approach in risk assessment for preeclampsia
development., Uprkos značajnom napretku u poboljšanju ishoda trudnoće poslednjih decenija,
predviđanje rizika i terapija preeklampsije su još uvijek veliki izazovi u kliničkoj praksi (1).
Cilj ove studije je bio ispitivanje biomarkera koji se ne koriste u rutinskoj praksi u proceni
rizika za razvoj preeklampsije. U studiji je učestvovalo 90 žena sa visokorizičnim
trudnoćama, od kojih je 20 razvilo preeklampsiju do kraja trudnoće. Biohemijski parametri
su određivani između 12. i 13. nedelje gestacije. Rezultati studije su pokazali da su žene koje
su razvile preeklampsiju imale više koncentracije latosterola, markera sinteze holesterola (p
<0,05), inflamatornih proteina - monocitnog hemoatraktantnog proteina-1 (MCP-1) i
rezistina (p < 0,01, oba), kao i aktivnost enzima paraoksonaze-1 (PON1) (p <0,05). Binarna
logistička regresiona analiza je pokazala da su više koncentracije latosterola, MCP-1,
rezistina i PON-1 povezane sa razvojem preeklampsije. Da bi se utvrdilo da su parametri koji
su se u univarijantnoj analizi pokazali značajnim, nezavisni prediktori preeklampsije,
primjenili smo multivarijantnu regresionu analizu. U model su ušli klinički parametri koji se
uobičajeno koriste u procjeni rizika (starost i indeks tjelesne mase majke, srednji arterijski
pritisak i protok krvi kroz matericu), latosterol, MCP-1, rezistin i PON-1. MCP-1 i rezistin su
se istakli kao značajni nezavisni prediktori preeklampsije. Pokazana je odlična dijagnostička
tačnost ispitivanog modela (AUC=0,859). Rezultati ove studije su ukazali na značaj
multimarkerskog pristupa u procjeni rizika za razvoj preeklampsije.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Novel biomarkers in preeclampsia risk assessment, Novi biomarkeri u procjeni rizika za razvoj preeklampsije",
volume = "72",
number = "4 suplement",
pages = "S620-S621",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4644"
}

Antonić, T., Ardalić, D., Vladimirov, S., Marković, G., Cabunac, P., Mihajlović, M., Miković, Ž.,& Stefanović, A.. (2022). Novel biomarkers in preeclampsia risk assessment. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S620-S621.
https://hdl.handle.net/21.15107/rcub_farfar_4644

Antonić T, Ardalić D, Vladimirov S, Marković G, Cabunac P, Mihajlović M, Miković Ž, Stefanović A. Novel biomarkers in preeclampsia risk assessment. in Arhiv za farmaciju. 2022;72(4 suplement):S620-S621.
https://hdl.handle.net/21.15107/rcub_farfar_4644 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Marković, Gorica, Cabunac, Petar, Mihajlović, Marija, Miković, Željko, Stefanović, Aleksandra, "Novel biomarkers in preeclampsia risk assessment" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S620-S621,
https://hdl.handle.net/21.15107/rcub_farfar_4644 .

TY  - JOUR
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Zeljković, Aleksandra
AU  - Karadžov-Orlić, Natasa
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4023
AB  - Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.
PB  - Medycyna Praktyczna Cholerzyn
T2  - Polish Archives of Internal Medicine
T1  - Cholesterol homeostasis is dysregulated in women with preeclampsia
VL  - 131
IS  - 12
DO  - 10.20452/pamw.16144
ER  - 

@article{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Banjac, Gorica and Cabunac, Petar and Zeljković, Aleksandra and Karadžov-Orlić, Natasa and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2021",
abstract = "Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.",
publisher = "Medycyna Praktyczna Cholerzyn",
journal = "Polish Archives of Internal Medicine",
title = "Cholesterol homeostasis is dysregulated in women with preeclampsia",
volume = "131",
number = "12",
doi = "10.20452/pamw.16144"
}

Antonić, T., Ardalić, D., Vladimirov, S., Banjac, G., Cabunac, P., Zeljković, A., Karadžov-Orlić, N., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2021). Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine
Medycyna Praktyczna Cholerzyn., 131(12).
https://doi.org/10.20452/pamw.16144

Antonić T, Ardalić D, Vladimirov S, Banjac G, Cabunac P, Zeljković A, Karadžov-Orlić N, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine. 2021;131(12).
doi:10.20452/pamw.16144 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Banjac, Gorica, Cabunac, Petar, Zeljković, Aleksandra, Karadžov-Orlić, Natasa, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Cholesterol homeostasis is dysregulated in women with preeclampsia" in Polish Archives of Internal Medicine, 131, no. 12 (2021),
https://doi.org/10.20452/pamw.16144 . .

TY  - CONF
AU  - Ivanišević, Jasmina
AU  - Ardalić, Daniela
AU  - Banjac, Gorica
AU  - Janać, Jelena
AU  - Cabunac, Petar
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4361
PB  - Elsevier B.V.
C3  - Free Radical Biology and Medicine
T1  - Antioxidant status in hypertensive disorders of pregnancy
VL  - 177
IS  - Supplement 1
SP  - S116
EP  - S117
DO  - 10.1016/j.freeradbiomed.2021.08.185
ER  - 

@conference{
author = "Ivanišević, Jasmina and Ardalić, Daniela and Banjac, Gorica and Janać, Jelena and Cabunac, Petar and Vekić, Jelena and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2021",
publisher = "Elsevier B.V.",
journal = "Free Radical Biology and Medicine",
title = "Antioxidant status in hypertensive disorders of pregnancy",
volume = "177",
number = "Supplement 1",
pages = "S116-S117",
doi = "10.1016/j.freeradbiomed.2021.08.185"
}

Ivanišević, J., Ardalić, D., Banjac, G., Janać, J., Cabunac, P., Vekić, J., Zeljković, A., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2021). Antioxidant status in hypertensive disorders of pregnancy. in Free Radical Biology and Medicine
Elsevier B.V.., 177(Supplement 1), S116-S117.
https://doi.org/10.1016/j.freeradbiomed.2021.08.185

Ivanišević J, Ardalić D, Banjac G, Janać J, Cabunac P, Vekić J, Zeljković A, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Antioxidant status in hypertensive disorders of pregnancy. in Free Radical Biology and Medicine. 2021;177(Supplement 1):S116-S117.
doi:10.1016/j.freeradbiomed.2021.08.185 .

Ivanišević, Jasmina, Ardalić, Daniela, Banjac, Gorica, Janać, Jelena, Cabunac, Petar, Vekić, Jelena, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Antioxidant status in hypertensive disorders of pregnancy" in Free Radical Biology and Medicine, 177, no. Supplement 1 (2021):S116-S117,
https://doi.org/10.1016/j.freeradbiomed.2021.08.185 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Miljković, Milica
AU  - Mandić-Marković, Vesna
AU  - Stanimirović, Srđan
AU  - Damnjanović Pažin, Barbara
AU  - Spasić, Slavíca
AU  - Spasojević-Kalimanovska, Vesna
AU  - Karadžov-Orlić, Nataša
AU  - Miković, Željko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3626
AB  - Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction
VL  - 81
SP  - 34
EP  - 40
DO  - 10.1016/j.clinbiochem.2020.05.003
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Banjac, Gorica and Cabunac, Petar and Miljković, Milica and Mandić-Marković, Vesna and Stanimirović, Srđan and Damnjanović Pažin, Barbara and Spasić, Slavíca and Spasojević-Kalimanovska, Vesna and Karadžov-Orlić, Nataša and Miković, Željko",
year = "2020",
abstract = "Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction",
volume = "81",
pages = "34-40",
doi = "10.1016/j.clinbiochem.2020.05.003"
}

Ardalić, D., Stefanović, A., Banjac, G., Cabunac, P., Miljković, M., Mandić-Marković, V., Stanimirović, S., Damnjanović Pažin, B., Spasić, S., Spasojević-Kalimanovska, V., Karadžov-Orlić, N.,& Miković, Ž.. (2020). Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry
Elsevier., 81, 34-40.
https://doi.org/10.1016/j.clinbiochem.2020.05.003

Ardalić D, Stefanović A, Banjac G, Cabunac P, Miljković M, Mandić-Marković V, Stanimirović S, Damnjanović Pažin B, Spasić S, Spasojević-Kalimanovska V, Karadžov-Orlić N, Miković Ž. Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry. 2020;81:34-40.
doi:10.1016/j.clinbiochem.2020.05.003 .

Ardalić, Daniela, Stefanović, Aleksandra, Banjac, Gorica, Cabunac, Petar, Miljković, Milica, Mandić-Marković, Vesna, Stanimirović, Srđan, Damnjanović Pažin, Barbara, Spasić, Slavíca, Spasojević-Kalimanovska, Vesna, Karadžov-Orlić, Nataša, Miković, Željko, "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction" in Clinical Biochemistry, 81 (2020):34-40,
https://doi.org/10.1016/j.clinbiochem.2020.05.003 . .