TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Klisić, Aleksandra
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Memon, Lidija
AU  - Gaković, Branka
AU  - Bogavac-Stanojević, Nataša
AU  - Spasojević-Kalimanovska, Vesna
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4677
AB  - Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.
PB  - Termedia Publishing House Ltd.
T2  - Archives of Medical Science
T1  - Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
VL  - 19
IS  - 2
SP  - 313
EP  - 323
DO  - 10.5114/aoms/136074
ER  - 

@article{
author = "Vukašinović, Aleksandra and Ostanek, Barbara and Klisić, Aleksandra and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Memon, Lidija and Gaković, Branka and Bogavac-Stanojević, Nataša and Spasojević-Kalimanovska, Vesna and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.",
publisher = "Termedia Publishing House Ltd.",
journal = "Archives of Medical Science",
title = "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress",
volume = "19",
number = "2",
pages = "313-323",
doi = "10.5114/aoms/136074"
}

Vukašinović, A., Ostanek, B., Klisić, A., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Memon, L., Gaković, B., Bogavac-Stanojević, N., Spasojević-Kalimanovska, V., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science
Termedia Publishing House Ltd.., 19(2), 313-323.
https://doi.org/10.5114/aoms/136074

Vukašinović A, Ostanek B, Klisić A, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Memon L, Gaković B, Bogavac-Stanojević N, Spasojević-Kalimanovska V, Marc J, Nešković A, Kotur-Stevuljević J. Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science. 2023;19(2):313-323.
doi:10.5114/aoms/136074 .

Vukašinović, Aleksandra, Ostanek, Barbara, Klisić, Aleksandra, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Memon, Lidija, Gaković, Branka, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress" in Archives of Medical Science, 19, no. 2 (2023):313-323,
https://doi.org/10.5114/aoms/136074 . .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Klisic, Aleksandra
AU  - Ostanek, Barbara
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Bogavac-Stanojević, Nataša
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5078
AB  - In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?
VL  - 24
IS  - 18
DO  - 10.3390/ijms241814308
ER  - 

@article{
author = "Vukašinović, Aleksandra and Klisic, Aleksandra and Ostanek, Barbara and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Bogavac-Stanojević, Nataša and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?",
volume = "24",
number = "18",
doi = "10.3390/ijms241814308"
}

Vukašinović, A., Klisic, A., Ostanek, B., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Bogavac-Stanojević, N., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences
MDPI., 24(18).
https://doi.org/10.3390/ijms241814308

Vukašinović A, Klisic A, Ostanek B, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Bogavac-Stanojević N, Marc J, Nešković A, Kotur-Stevuljević J. Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences. 2023;24(18).
doi:10.3390/ijms241814308 .

Vukašinović, Aleksandra, Klisic, Aleksandra, Ostanek, Barbara, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Bogavac-Stanojević, Nataša, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?" in International Journal of Molecular Sciences, 24, no. 18 (2023),
https://doi.org/10.3390/ijms241814308 . .

TY  - JOUR
AU  - Belić, Milica
AU  - Sopić, Miron
AU  - Roksandić-Milenković, Marina
AU  - Ćeriman, Vesna
AU  - Guzonjić, Azra
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Dimić, Nemanja
AU  - Jovanović, Dragana
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5387
AB  - Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.
PB  - Charles University, Czech Republic
T2  - Folia biologica
T1  - Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis
VL  - 69
IS  - 2
SP  - 59
EP  - 68
DO  - 10.14712/fb2023069020059
ER  - 

@article{
author = "Belić, Milica and Sopić, Miron and Roksandić-Milenković, Marina and Ćeriman, Vesna and Guzonjić, Azra and Vukašinović, Aleksandra and Ostanek, Barbara and Dimić, Nemanja and Jovanović, Dragana and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.",
publisher = "Charles University, Czech Republic",
journal = "Folia biologica",
title = "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis",
volume = "69",
number = "2",
pages = "59-68",
doi = "10.14712/fb2023069020059"
}

Belić, M., Sopić, M., Roksandić-Milenković, M., Ćeriman, V., Guzonjić, A., Vukašinović, A., Ostanek, B., Dimić, N., Jovanović, D.,& Kotur-Stevuljević, J.. (2023). Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica
Charles University, Czech Republic., 69(2), 59-68.
https://doi.org/10.14712/fb2023069020059

Belić M, Sopić M, Roksandić-Milenković M, Ćeriman V, Guzonjić A, Vukašinović A, Ostanek B, Dimić N, Jovanović D, Kotur-Stevuljević J. Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica. 2023;69(2):59-68.
doi:10.14712/fb2023069020059 .

Belić, Milica, Sopić, Miron, Roksandić-Milenković, Marina, Ćeriman, Vesna, Guzonjić, Azra, Vukašinović, Aleksandra, Ostanek, Barbara, Dimić, Nemanja, Jovanović, Dragana, Kotur-Stevuljević, Jelena, "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis" in Folia biologica, 69, no. 2 (2023):59-68,
https://doi.org/10.14712/fb2023069020059 . .

TY  - JOUR
AU  - Vuković Dejanović, Vesna
AU  - Kotur-Stevuljević, Jelena
AU  - Vukašinović, Aleksandra
AU  - Miljković, Milica
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Hinić, Saša
AU  - Cerović, Milivoje
AU  - Stefanović, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Memon, Lidija
AU  - Nešković, Aleksandar
AU  - Bogavac-Stanojević, Nataša
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3612
AB  - We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.
PB  - SAGE journals
T2  - Angiology
T1  - Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?
VL  - 71
IS  - 8
SP  - 713
EP  - 720
DO  - 10.1177/0003319720921724
ER  - 

@article{
author = "Vuković Dejanović, Vesna and Kotur-Stevuljević, Jelena and Vukašinović, Aleksandra and Miljković, Milica and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Spasojević-Kalimanovska, Vesna and Memon, Lidija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša",
year = "2020",
abstract = "We investigated circulating levels of inflammatory biomarkers pentraxin-3 (PTX3), cyclophilin A (CypA), and heparin-binding epidermal growth factor-like growth factor (HB-EGF); oxidative stress; and antioxidant status markers in the patients with ST-segment elevation acute myocardial infarction (STEMI) to better understand a relationship between inflammation and oxidative stress. We examined the impact of oxidative stress on high values of inflammatory parameters. The study included 87 patients with STEMI and 193 controls. We observed a positive correlation between PTX3 and HB-EGF (ρ = 0.24, P = .027), CyPA, and sulfhydryl (SH) groups (ρ = 0.25, P = .026), and a negative correlation between PTX3 and SH groups (ρ = −0.35, P = .001) in patients with STEMI. To better understand the effect of the examined parameters on the occurrence of high concentrations of inflammatory parameters, we grouped them using principal component analysis. This analysis identified the 4 most contributing factors. Optimal cutoff values for discrimination of patients with STEMI from controls were calculated for PTX3 and HB-EGF. An independent predictor for PTX3 above the cutoff value was a “metabolic-oxidative stress factor” comprised of glucose and oxidative stress marker prooxidant-antioxidant balance (odds ratio = 4.449, P = .030). The results show that higher PTX3 values will occur in patients having STEMI with greater metabolic and oxidative stress status values.",
publisher = "SAGE journals",
journal = "Angiology",
title = "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?",
volume = "71",
number = "8",
pages = "713-720",
doi = "10.1177/0003319720921724"
}

Vuković Dejanović, V., Kotur-Stevuljević, J., Vukašinović, A., Miljković, M., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Spasojević-Kalimanovska, V., Memon, L., Nešković, A.,& Bogavac-Stanojević, N.. (2020). Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology
SAGE journals., 71(8), 713-720.
https://doi.org/10.1177/0003319720921724

Vuković Dejanović V, Kotur-Stevuljević J, Vukašinović A, Miljković M, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Spasojević-Kalimanovska V, Memon L, Nešković A, Bogavac-Stanojević N. Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?. in Angiology. 2020;71(8):713-720.
doi:10.1177/0003319720921724 .

Vuković Dejanović, Vesna, Kotur-Stevuljević, Jelena, Vukašinović, Aleksandra, Miljković, Milica, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Spasojević-Kalimanovska, Vesna, Memon, Lidija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "Oxidative Stress and Inflammatory Markers PTX3, CypA, and HB-EGF: How Are They Linked in Patients With STEMI?" in Angiology, 71, no. 8 (2020):713-720,
https://doi.org/10.1177/0003319720921724 . .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Mlakar, Vid
AU  - Sopić, Miron
AU  - Cvetković, Zorica P.
AU  - Petković, Miloš
AU  - Spasojević-Kalimanovska, Vesna
AU  - Bogavac-Stanojević, Nataša
AU  - Ostanek, Barbara
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3356
AB  - Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p  lt  .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p  lt  .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Telomerase stability and evaluation of real-time telomeric repeat amplification protocol
VL  - 79
IS  - 3
SP  - 188
EP  - 193
DO  - 10.1080/00365513.2019.1576220
ER  - 

@article{
author = "Vukašinović, Aleksandra and Kotur-Stevuljević, Jelena and Mlakar, Vid and Sopić, Miron and Cvetković, Zorica P. and Petković, Miloš and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Ostanek, Barbara",
year = "2019",
abstract = "Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p  lt  .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p  lt  .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Telomerase stability and evaluation of real-time telomeric repeat amplification protocol",
volume = "79",
number = "3",
pages = "188-193",
doi = "10.1080/00365513.2019.1576220"
}

Vukašinović, A., Kotur-Stevuljević, J., Mlakar, V., Sopić, M., Cvetković, Z. P., Petković, M., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N.,& Ostanek, B.. (2019). Telomerase stability and evaluation of real-time telomeric repeat amplification protocol. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 79(3), 188-193.
https://doi.org/10.1080/00365513.2019.1576220

Vukašinović A, Kotur-Stevuljević J, Mlakar V, Sopić M, Cvetković ZP, Petković M, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Ostanek B. Telomerase stability and evaluation of real-time telomeric repeat amplification protocol. in Scandinavian Journal of Clinical and Laboratory Investigation. 2019;79(3):188-193.
doi:10.1080/00365513.2019.1576220 .

Vukašinović, Aleksandra, Kotur-Stevuljević, Jelena, Mlakar, Vid, Sopić, Miron, Cvetković, Zorica P., Petković, Miloš, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Ostanek, Barbara, "Telomerase stability and evaluation of real-time telomeric repeat amplification protocol" in Scandinavian Journal of Clinical and Laboratory Investigation, 79, no. 3 (2019):188-193,
https://doi.org/10.1080/00365513.2019.1576220 . .

TY  - JOUR
AU  - Paripović, Dušan
AU  - Kotur-Stevuljević, Jelena
AU  - Vukašinović, Aleksandra
AU  - Ilisić, Tamara
AU  - Miloševski-Lomić, Gordana
AU  - Peco-Antić, Amira
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3144
AB  - Oxidative stress seems to be an important link between obesity and cardiovascular disease. The aim of our study was to assess oxidative stress in obese patients stratified according to ambulatory blood pressure status and to determine independent predictors of abnormal left ventricular geometry.A cross-sectional study was conducted. A total of 113 obese participants referred for 24-h ambulatory blood pressure monitoring (ABPM) aged 9-19 years, and 29 healthy controls were enrolled. In addition to anthropometric and biochemical measurements, such as fasting serum levels of glucose, insulin, lipid profile, and oxidative biomarkers, ABPM and echocardiography were performed.According to ABPM results, obese subjects were split in two groups: 57 hypertensive and 56 normotensive. Both hypertensive and normotensive obese participants had higher levels of oxidative stress parameters (pro-oxidative/antioxidative balance and total oxidant status) compared with control group. Levels of superoxide anion (O-2(-)) and sulfhydryl groups were higher in obese hypertensive participants as compared to obese normotensive and control groups. Abnormal left ventricular geometry among obese participants was independently associated with O-2(-) (p = .006) and body mass index z score (p = .020), with no significant impact of gender, while age and systolic blood pressure exhibited interaction term for the outcome.The independent effect of oxidative mechanisms on left ventricular geometry appears to start in childhood. Oxidative stress occurs in obese adolescents prior to the development of sustained hypertension.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - The influence of oxidative stress on cardiac remodeling in obese adolescents
VL  - 78
IS  - 7-8
SP  - 595
EP  - 600
DO  - 10.1080/00365513.2018.1528504
ER  - 

@article{
author = "Paripović, Dušan and Kotur-Stevuljević, Jelena and Vukašinović, Aleksandra and Ilisić, Tamara and Miloševski-Lomić, Gordana and Peco-Antić, Amira",
year = "2018",
abstract = "Oxidative stress seems to be an important link between obesity and cardiovascular disease. The aim of our study was to assess oxidative stress in obese patients stratified according to ambulatory blood pressure status and to determine independent predictors of abnormal left ventricular geometry.A cross-sectional study was conducted. A total of 113 obese participants referred for 24-h ambulatory blood pressure monitoring (ABPM) aged 9-19 years, and 29 healthy controls were enrolled. In addition to anthropometric and biochemical measurements, such as fasting serum levels of glucose, insulin, lipid profile, and oxidative biomarkers, ABPM and echocardiography were performed.According to ABPM results, obese subjects were split in two groups: 57 hypertensive and 56 normotensive. Both hypertensive and normotensive obese participants had higher levels of oxidative stress parameters (pro-oxidative/antioxidative balance and total oxidant status) compared with control group. Levels of superoxide anion (O-2(-)) and sulfhydryl groups were higher in obese hypertensive participants as compared to obese normotensive and control groups. Abnormal left ventricular geometry among obese participants was independently associated with O-2(-) (p = .006) and body mass index z score (p = .020), with no significant impact of gender, while age and systolic blood pressure exhibited interaction term for the outcome.The independent effect of oxidative mechanisms on left ventricular geometry appears to start in childhood. Oxidative stress occurs in obese adolescents prior to the development of sustained hypertension.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "The influence of oxidative stress on cardiac remodeling in obese adolescents",
volume = "78",
number = "7-8",
pages = "595-600",
doi = "10.1080/00365513.2018.1528504"
}

Paripović, D., Kotur-Stevuljević, J., Vukašinović, A., Ilisić, T., Miloševski-Lomić, G.,& Peco-Antić, A.. (2018). The influence of oxidative stress on cardiac remodeling in obese adolescents. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 78(7-8), 595-600.
https://doi.org/10.1080/00365513.2018.1528504

Paripović D, Kotur-Stevuljević J, Vukašinović A, Ilisić T, Miloševski-Lomić G, Peco-Antić A. The influence of oxidative stress on cardiac remodeling in obese adolescents. in Scandinavian Journal of Clinical and Laboratory Investigation. 2018;78(7-8):595-600.
doi:10.1080/00365513.2018.1528504 .

Paripović, Dušan, Kotur-Stevuljević, Jelena, Vukašinović, Aleksandra, Ilisić, Tamara, Miloševski-Lomić, Gordana, Peco-Antić, Amira, "The influence of oxidative stress on cardiac remodeling in obese adolescents" in Scandinavian Journal of Clinical and Laboratory Investigation, 78, no. 7-8 (2018):595-600,
https://doi.org/10.1080/00365513.2018.1528504 . .

TY  - CONF
AU  - Malenica, M.
AU  - Smailbegović, H.
AU  - Bego, T.
AU  - Dujić, T.
AU  - Causević, A.
AU  - Semiz, S.
AU  - Vukašinović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2787
PB  - Wiley, Hoboken
C3  - FEBS Journal
T1  - Testing the prooxidant-antioxidant balance in patients with prediabetes and Type 2 diabetes mellitus
VL  - 284
IS  - Supplement 1
SP  - 329
EP  - 329
DO  - 10.1111/febs.14174
ER  - 

@conference{
author = "Malenica, M. and Smailbegović, H. and Bego, T. and Dujić, T. and Causević, A. and Semiz, S. and Vukašinović, Aleksandra and Kotur-Stevuljević, Jelena",
year = "2017",
publisher = "Wiley, Hoboken",
journal = "FEBS Journal",
title = "Testing the prooxidant-antioxidant balance in patients with prediabetes and Type 2 diabetes mellitus",
volume = "284",
number = "Supplement 1",
pages = "329-329",
doi = "10.1111/febs.14174"
}

Malenica, M., Smailbegović, H., Bego, T., Dujić, T., Causević, A., Semiz, S., Vukašinović, A.,& Kotur-Stevuljević, J.. (2017). Testing the prooxidant-antioxidant balance in patients with prediabetes and Type 2 diabetes mellitus. in FEBS Journal
Wiley, Hoboken., 284(Supplement 1), 329-329.
https://doi.org/10.1111/febs.14174

Malenica M, Smailbegović H, Bego T, Dujić T, Causević A, Semiz S, Vukašinović A, Kotur-Stevuljević J. Testing the prooxidant-antioxidant balance in patients with prediabetes and Type 2 diabetes mellitus. in FEBS Journal. 2017;284(Supplement 1):329-329.
doi:10.1111/febs.14174 .

Malenica, M., Smailbegović, H., Bego, T., Dujić, T., Causević, A., Semiz, S., Vukašinović, Aleksandra, Kotur-Stevuljević, Jelena, "Testing the prooxidant-antioxidant balance in patients with prediabetes and Type 2 diabetes mellitus" in FEBS Journal, 284, no. Supplement 1 (2017):329-329,
https://doi.org/10.1111/febs.14174 . .

TY  - CONF
AU  - Vukašinović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Stefanović, Aleksandra
AU  - Spasić, S.
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ristić, D.
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1406
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis Supplements
T1  - Total antioxidant status in coronary artery disease patients
VL  - 11
IS  - 2
SP  - 197
EP  - 197
DO  - 10.1016/S1567-5688(10)70934-X
ER  - 

@conference{
author = "Vukašinović, Aleksandra and Kotur-Stevuljević, Jelena and Stefanović, Aleksandra and Spasić, S. and Spasojević-Kalimanovska, Vesna and Ristić, D.",
year = "2010",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis Supplements",
title = "Total antioxidant status in coronary artery disease patients",
volume = "11",
number = "2",
pages = "197-197",
doi = "10.1016/S1567-5688(10)70934-X"
}

Vukašinović, A., Kotur-Stevuljević, J., Stefanović, A., Spasić, S., Spasojević-Kalimanovska, V.,& Ristić, D.. (2010). Total antioxidant status in coronary artery disease patients. in Atherosclerosis Supplements
Elsevier Ireland Ltd, Clare., 11(2), 197-197.
https://doi.org/10.1016/S1567-5688(10)70934-X

Vukašinović A, Kotur-Stevuljević J, Stefanović A, Spasić S, Spasojević-Kalimanovska V, Ristić D. Total antioxidant status in coronary artery disease patients. in Atherosclerosis Supplements. 2010;11(2):197-197.
doi:10.1016/S1567-5688(10)70934-X .

Vukašinović, Aleksandra, Kotur-Stevuljević, Jelena, Stefanović, Aleksandra, Spasić, S., Spasojević-Kalimanovska, Vesna, Ristić, D., "Total antioxidant status in coronary artery disease patients" in Atherosclerosis Supplements, 11, no. 2 (2010):197-197,
https://doi.org/10.1016/S1567-5688(10)70934-X . .