TY  - JOUR
AU  - Milosavljević, Filip
AU  - Brusini, Irene
AU  - Atanasov, Andrea
AU  - Manojlović, Marina
AU  - Vučić, Marija
AU  - Oreščanin-Dušić, Zorana
AU  - Brkljačić, Jelena
AU  - Miljević, Čedo
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Wang, Chunliang
AU  - Damberg, Peter
AU  - Pešić, Vesna
AU  - Tyndale, Rachel F.
AU  - Ingelman-Sundberg, Magnus
AU  - Jukić, Marin
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4516
AB  - Aims: CYP2C19 transgenic mouse expresses the human CYP2C19 gene in the liver and developing brain, and it exhibits altered neurodevelopment associated with impairments in emotionality and locomotion. Because the validation of new animal models is essential for the understanding of the aetiology and pathophysiology of movement disorders, the objective was to characterise motoric phenotype in CYP2C19 transgenic mice and to investigate its validity as a new animal model of ataxia. Methods: The rotarod, paw-print and beam-walking tests were utilised to characterise the motoric phenotype. The volumes of 20 brain regions in CYP2C19 transgenic and wild-type mice were quantified by 9.4T gadolinium-enhanced post-mortem structural neuroimaging. Antioxidative enzymatic activity was quantified biochemically. Dopaminergic alterations were characterised by chromatographic quantification of concentrations of dopamine and its metabolites and by subsequent immunohistochemical analyses. The beam-walking test was repeated after the treatment with dopamine receptor antagonists ecopipam and raclopride. Results: CYP2C19 transgenic mice exhibit abnormal, unilateral ataxia-like gait, clasping reflex and 5.6-fold more paw-slips in the beam-walking test; the motoric phenotype was more pronounced in youth. Transgenic mice exhibited a profound reduction of 12% in cerebellar volume and a moderate reduction of 4% in hippocampal volume; both regions exhibited an increased antioxidative enzyme activity. CYP2C19 mice were hyperdopaminergic; however, the motoric impairment was not ameliorated by dopamine receptor antagonists, and there was no alteration in the number of midbrain dopaminergic neurons in CYP2C19 mice. Conclusions: Humanised CYP2C19 transgenic mice exhibit altered gait and functional motoric impairments; this phenotype is likely caused by an aberrant cerebellar development.
PB  - John Wiley and Sons Inc
T2  - Neuropathology and Applied Neurobiology
T1  - The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia
VL  - 49
IS  - 1
DO  - 10.1111/nan.12867
ER  - 

@article{
author = "Milosavljević, Filip and Brusini, Irene and Atanasov, Andrea and Manojlović, Marina and Vučić, Marija and Oreščanin-Dušić, Zorana and Brkljačić, Jelena and Miljević, Čedo and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Wang, Chunliang and Damberg, Peter and Pešić, Vesna and Tyndale, Rachel F. and Ingelman-Sundberg, Magnus and Jukić, Marin",
year = "2023",
abstract = "Aims: CYP2C19 transgenic mouse expresses the human CYP2C19 gene in the liver and developing brain, and it exhibits altered neurodevelopment associated with impairments in emotionality and locomotion. Because the validation of new animal models is essential for the understanding of the aetiology and pathophysiology of movement disorders, the objective was to characterise motoric phenotype in CYP2C19 transgenic mice and to investigate its validity as a new animal model of ataxia. Methods: The rotarod, paw-print and beam-walking tests were utilised to characterise the motoric phenotype. The volumes of 20 brain regions in CYP2C19 transgenic and wild-type mice were quantified by 9.4T gadolinium-enhanced post-mortem structural neuroimaging. Antioxidative enzymatic activity was quantified biochemically. Dopaminergic alterations were characterised by chromatographic quantification of concentrations of dopamine and its metabolites and by subsequent immunohistochemical analyses. The beam-walking test was repeated after the treatment with dopamine receptor antagonists ecopipam and raclopride. Results: CYP2C19 transgenic mice exhibit abnormal, unilateral ataxia-like gait, clasping reflex and 5.6-fold more paw-slips in the beam-walking test; the motoric phenotype was more pronounced in youth. Transgenic mice exhibited a profound reduction of 12% in cerebellar volume and a moderate reduction of 4% in hippocampal volume; both regions exhibited an increased antioxidative enzyme activity. CYP2C19 mice were hyperdopaminergic; however, the motoric impairment was not ameliorated by dopamine receptor antagonists, and there was no alteration in the number of midbrain dopaminergic neurons in CYP2C19 mice. Conclusions: Humanised CYP2C19 transgenic mice exhibit altered gait and functional motoric impairments; this phenotype is likely caused by an aberrant cerebellar development.",
publisher = "John Wiley and Sons Inc",
journal = "Neuropathology and Applied Neurobiology",
title = "The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia",
volume = "49",
number = "1",
doi = "10.1111/nan.12867"
}

Milosavljević, F., Brusini, I., Atanasov, A., Manojlović, M., Vučić, M., Oreščanin-Dušić, Z., Brkljačić, J., Miljević, Č., Nikolić-Kokić, A., Blagojević, D., Wang, C., Damberg, P., Pešić, V., Tyndale, R. F., Ingelman-Sundberg, M.,& Jukić, M.. (2023). The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia. in Neuropathology and Applied Neurobiology
John Wiley and Sons Inc., 49(1).
https://doi.org/10.1111/nan.12867

Milosavljević F, Brusini I, Atanasov A, Manojlović M, Vučić M, Oreščanin-Dušić Z, Brkljačić J, Miljević Č, Nikolić-Kokić A, Blagojević D, Wang C, Damberg P, Pešić V, Tyndale RF, Ingelman-Sundberg M, Jukić M. The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia. in Neuropathology and Applied Neurobiology. 2023;49(1).
doi:10.1111/nan.12867 .

Milosavljević, Filip, Brusini, Irene, Atanasov, Andrea, Manojlović, Marina, Vučić, Marija, Oreščanin-Dušić, Zorana, Brkljačić, Jelena, Miljević, Čedo, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Wang, Chunliang, Damberg, Peter, Pešić, Vesna, Tyndale, Rachel F., Ingelman-Sundberg, Magnus, Jukić, Marin, "The humanised CYP2C19 transgenic mouse exhibits cerebellar atrophy and movement impairment reminiscent of ataxia" in Neuropathology and Applied Neurobiology, 49, no. 1 (2023),
https://doi.org/10.1111/nan.12867 . .

TY  - CONF
AU  - Milosavljević, Filip
AU  - Brusini, Irene
AU  - Atanasov, Andrea
AU  - Manojlović, Marina
AU  - Vučić, Marija
AU  - Oreščanin-Dušić, Zorana
AU  - Brkljačić, Jelena
AU  - Sitarica, Pavle
AU  - Miljević, Čedo
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Wang, Chunliang
AU  - Damberg, Peter
AU  - Pešić, Vesna
AU  - Tyndale, Rachel
AU  - Ingelman-Sundberg, Magnus
AU  - Jukić, Marin
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4729
AB  - Background: Animal models are essential for understanding aetiology and pathophysiology of movement disorders. Previously, it had been found that mice transgenic for the human CYP2C19 gene, which is expressed in the liver and developing brain, exhibit altered neurodevelopment associated with impairments of their motor function and emotionality [1, 2]. The aim of this study was to characterize motoric phenotype of the CYP2C19 transgenic mouse and validate its potential usefulness as an animal model for ataxia.

Methods: Experiments were performed on CYP2C19 transgenic mice and control wild-type littermate mice. Body weight of mice was measured between the 21st and 42nd postnatal day. Mouse gait was analysed with footprint test [3] in young animals at four time points and once in adult mice. The maximal height of hindpaw elevation while walking was measured offline from the video footage of the footprint test. Motoric function was quantified by the rotarod and beam-walking tests. Structural differences in 20 brain regions of wild-type and transgenic mice were investigated with 9.4T gadolinium-enhanced postmortem neuroimaging. Antioxidative enzyme status was determined in the brain tissue in order to assess potential differences in the brain oxidative-antioxidative balance between wild-type and transgenic mice. When multiple brain regions or multiple antioxidant enzyme activities were analysed, p-values were FDR corrected for multiple comparisons.

Results: CYP2C19 transgenic (TG) animals exhibited approximately 5-10% reduced body weight (p=0.015) during 3rd and 4th postnatal week, while after postnatal day 31, the differences in the body weight were no longer statistically significant. The TG animals exhibited approximately two fold higher maximal hindpaw elevation in young (2.1-fold [CI95%: 2.0, 2.2], p<0.0001) and adult mice (1.9-fold [CI95%: 1.8, 2.1], p<0.0001), compared to wild-types. In the 5th postnatal week, all transgenic mice exhibited increase in elevation of both hindpaws, while after this point they gradually started exhibiting unilateral phenotype until almost all (49 of 51) animals became unilaterally affected in the adulthood. Footprint analysis and rotarod test did not detect significant differences (p>0.1) between TG and control mice in any of the analysed parameters, accounting for all examined time points. CYP2C19 transgenic mice exhibited 14% increase in beam crossing time (14%, [95%CI: 6.4, 22], p=0.0014) and 5.6-fold more paw-slips (p<0.0001, n=89) in the beam-walking test. CYP2C19 transgenic mice exhibited profound reduction in cerebellar volume (-11.8% [95%CI: -14.7, -9.0], q<0.0001, n=59) and moderate reduction in hippocampal volume (-4.2% [95%CI: -6.4%, -1.9%], q=0.015, n=59); compared to the corresponding volumes measured in WT mice. Superoxide dismutase activity was slightly increased (1.14-fold [CI95%: 1.06, 1.23], p=0.0010, q=0.023) in the cerebelli and moderately increased (1.3-fold, [CI95%: 1.18, 1.47], p<0.0001, q=0.0013) in the hippocampi of transgenic mice compared to wild-types, while glutathione reductase activity was significantly increased (1.2-fold [CI95%: 1.13, 1.35], p<0.0001, q=0.0021) in the hippocampi of TG mice.

Conclusions: Humanized CYP2C19 transgenic mice exhibit altered motoric function, functional motoric impairments and reduced cerebellar volume. CYP2C19 transgenic mice can be a useful tool for the studies focused on understanding the physiology of cerebellar development, as well as aetiology and pathophysiology of cerebellum-related disorders.
PB  - Elsevier
C3  - Neuroscience Applied
T1  - Humanized CYP2C19 transgenic mouse as an animal model of cerebellar ataxia
VL  - 1
IS  - Supplement 2
SP  - 64
EP  - 64
DO  - 10.1016/j.nsa.2022.100236
ER  - 

@conference{
author = "Milosavljević, Filip and Brusini, Irene and Atanasov, Andrea and Manojlović, Marina and Vučić, Marija and Oreščanin-Dušić, Zorana and Brkljačić, Jelena and Sitarica, Pavle and Miljević, Čedo and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Wang, Chunliang and Damberg, Peter and Pešić, Vesna and Tyndale, Rachel and Ingelman-Sundberg, Magnus and Jukić, Marin",
year = "2022",
abstract = "Background: Animal models are essential for understanding aetiology and pathophysiology of movement disorders. Previously, it had been found that mice transgenic for the human CYP2C19 gene, which is expressed in the liver and developing brain, exhibit altered neurodevelopment associated with impairments of their motor function and emotionality [1, 2]. The aim of this study was to characterize motoric phenotype of the CYP2C19 transgenic mouse and validate its potential usefulness as an animal model for ataxia.

Methods: Experiments were performed on CYP2C19 transgenic mice and control wild-type littermate mice. Body weight of mice was measured between the 21st and 42nd postnatal day. Mouse gait was analysed with footprint test [3] in young animals at four time points and once in adult mice. The maximal height of hindpaw elevation while walking was measured offline from the video footage of the footprint test. Motoric function was quantified by the rotarod and beam-walking tests. Structural differences in 20 brain regions of wild-type and transgenic mice were investigated with 9.4T gadolinium-enhanced postmortem neuroimaging. Antioxidative enzyme status was determined in the brain tissue in order to assess potential differences in the brain oxidative-antioxidative balance between wild-type and transgenic mice. When multiple brain regions or multiple antioxidant enzyme activities were analysed, p-values were FDR corrected for multiple comparisons.

Results: CYP2C19 transgenic (TG) animals exhibited approximately 5-10% reduced body weight (p=0.015) during 3rd and 4th postnatal week, while after postnatal day 31, the differences in the body weight were no longer statistically significant. The TG animals exhibited approximately two fold higher maximal hindpaw elevation in young (2.1-fold [CI95%: 2.0, 2.2], p<0.0001) and adult mice (1.9-fold [CI95%: 1.8, 2.1], p<0.0001), compared to wild-types. In the 5th postnatal week, all transgenic mice exhibited increase in elevation of both hindpaws, while after this point they gradually started exhibiting unilateral phenotype until almost all (49 of 51) animals became unilaterally affected in the adulthood. Footprint analysis and rotarod test did not detect significant differences (p>0.1) between TG and control mice in any of the analysed parameters, accounting for all examined time points. CYP2C19 transgenic mice exhibited 14% increase in beam crossing time (14%, [95%CI: 6.4, 22], p=0.0014) and 5.6-fold more paw-slips (p<0.0001, n=89) in the beam-walking test. CYP2C19 transgenic mice exhibited profound reduction in cerebellar volume (-11.8% [95%CI: -14.7, -9.0], q<0.0001, n=59) and moderate reduction in hippocampal volume (-4.2% [95%CI: -6.4%, -1.9%], q=0.015, n=59); compared to the corresponding volumes measured in WT mice. Superoxide dismutase activity was slightly increased (1.14-fold [CI95%: 1.06, 1.23], p=0.0010, q=0.023) in the cerebelli and moderately increased (1.3-fold, [CI95%: 1.18, 1.47], p<0.0001, q=0.0013) in the hippocampi of transgenic mice compared to wild-types, while glutathione reductase activity was significantly increased (1.2-fold [CI95%: 1.13, 1.35], p<0.0001, q=0.0021) in the hippocampi of TG mice.

Conclusions: Humanized CYP2C19 transgenic mice exhibit altered motoric function, functional motoric impairments and reduced cerebellar volume. CYP2C19 transgenic mice can be a useful tool for the studies focused on understanding the physiology of cerebellar development, as well as aetiology and pathophysiology of cerebellum-related disorders.",
publisher = "Elsevier",
journal = "Neuroscience Applied",
title = "Humanized CYP2C19 transgenic mouse as an animal model of cerebellar ataxia",
volume = "1",
number = "Supplement 2",
pages = "64-64",
doi = "10.1016/j.nsa.2022.100236"
}

Milosavljević, F., Brusini, I., Atanasov, A., Manojlović, M., Vučić, M., Oreščanin-Dušić, Z., Brkljačić, J., Sitarica, P., Miljević, Č., Nikolić-Kokić, A., Blagojević, D., Wang, C., Damberg, P., Pešić, V., Tyndale, R., Ingelman-Sundberg, M.,& Jukić, M.. (2022). Humanized CYP2C19 transgenic mouse as an animal model of cerebellar ataxia. in Neuroscience Applied
Elsevier., 1(Supplement 2), 64-64.
https://doi.org/10.1016/j.nsa.2022.100236

Milosavljević F, Brusini I, Atanasov A, Manojlović M, Vučić M, Oreščanin-Dušić Z, Brkljačić J, Sitarica P, Miljević Č, Nikolić-Kokić A, Blagojević D, Wang C, Damberg P, Pešić V, Tyndale R, Ingelman-Sundberg M, Jukić M. Humanized CYP2C19 transgenic mouse as an animal model of cerebellar ataxia. in Neuroscience Applied. 2022;1(Supplement 2):64-64.
doi:10.1016/j.nsa.2022.100236 .

Milosavljević, Filip, Brusini, Irene, Atanasov, Andrea, Manojlović, Marina, Vučić, Marija, Oreščanin-Dušić, Zorana, Brkljačić, Jelena, Sitarica, Pavle, Miljević, Čedo, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Wang, Chunliang, Damberg, Peter, Pešić, Vesna, Tyndale, Rachel, Ingelman-Sundberg, Magnus, Jukić, Marin, "Humanized CYP2C19 transgenic mouse as an animal model of cerebellar ataxia" in Neuroscience Applied, 1, no. Supplement 2 (2022):64-64,
https://doi.org/10.1016/j.nsa.2022.100236 . .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško P.
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3648
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško P. and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D. P.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević DP, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško P., Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph" in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021),
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - JOUR
AU  - Miljević, Čedo D.
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Milovanović, Maja
AU  - Munjiza, Ana
AU  - Jukić, Marin
AU  - Pešić, Vesna
AU  - Lecić-Tosevski, Dušica
AU  - Spasić, Mihajlo B.
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3058
AB  - To determine the relationship between alterations in the activity of the enzymes participating in antioxidative defense system and neurological soft signs (NSS) in schizophrenic patients with the first episode psychosis (S-FE, n = 19), patients in relapse (S-R, n = 46), and healthy controls (HC, n = 20). NSS intensity and enzymatic plasma activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were compared between S-FE, S-R and HC subjects and a follow-up correlation analyses between the enzyme activities and NSS intensity was performed. NSS intensity was increased four times in schizophrenic patients compared with healthy controls. Activities of SOD and CAT were 40% decreased in S-FE and these reductions were ameliorated by antipsychotic treatment. GPX activity was 20% decreased in both patient groups compared with controls. A negative correlation between NSS intensity and GPX activity was specifically found in the S-FE patients. The data in this report argue that a reduction of GPX activity might be one of the causes for the emergence of NSS at the onset of schizophrenia, and provide the evidence that antipsychotic therapy can attenuate activity reductions of SOD and CAT, but not the activity reduction of GPX and the intensity of NSS.
PB  - Elsevier Ireland Ltd, Clare
T2  - Psychoneuroendocrinology
T1  - Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients
VL  - 269
SP  - 746
EP  - 752
DO  - 10.1016/j.psychres.2018.09.009
ER  - 

@article{
author = "Miljević, Čedo D. and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Milovanović, Maja and Munjiza, Ana and Jukić, Marin and Pešić, Vesna and Lecić-Tosevski, Dušica and Spasić, Mihajlo B.",
year = "2018",
abstract = "To determine the relationship between alterations in the activity of the enzymes participating in antioxidative defense system and neurological soft signs (NSS) in schizophrenic patients with the first episode psychosis (S-FE, n = 19), patients in relapse (S-R, n = 46), and healthy controls (HC, n = 20). NSS intensity and enzymatic plasma activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were compared between S-FE, S-R and HC subjects and a follow-up correlation analyses between the enzyme activities and NSS intensity was performed. NSS intensity was increased four times in schizophrenic patients compared with healthy controls. Activities of SOD and CAT were 40% decreased in S-FE and these reductions were ameliorated by antipsychotic treatment. GPX activity was 20% decreased in both patient groups compared with controls. A negative correlation between NSS intensity and GPX activity was specifically found in the S-FE patients. The data in this report argue that a reduction of GPX activity might be one of the causes for the emergence of NSS at the onset of schizophrenia, and provide the evidence that antipsychotic therapy can attenuate activity reductions of SOD and CAT, but not the activity reduction of GPX and the intensity of NSS.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Psychoneuroendocrinology",
title = "Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients",
volume = "269",
pages = "746-752",
doi = "10.1016/j.psychres.2018.09.009"
}

Miljević, Č. D., Nikolić-Kokić, A., Blagojević, D., Milovanović, M., Munjiza, A., Jukić, M., Pešić, V., Lecić-Tosevski, D.,& Spasić, M. B.. (2018). Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients. in Psychoneuroendocrinology
Elsevier Ireland Ltd, Clare., 269, 746-752.
https://doi.org/10.1016/j.psychres.2018.09.009

Miljević ČD, Nikolić-Kokić A, Blagojević D, Milovanović M, Munjiza A, Jukić M, Pešić V, Lecić-Tosevski D, Spasić MB. Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients. in Psychoneuroendocrinology. 2018;269:746-752.
doi:10.1016/j.psychres.2018.09.009 .

Miljević, Čedo D., Nikolić-Kokić, Aleksandra, Blagojević, Duško, Milovanović, Maja, Munjiza, Ana, Jukić, Marin, Pešić, Vesna, Lecić-Tosevski, Dušica, Spasić, Mihajlo B., "Association between neurological soft signs and antioxidant enzyme activity in schizophrenic patients" in Psychoneuroendocrinology, 269 (2018):746-752,
https://doi.org/10.1016/j.psychres.2018.09.009 . .

TY  - JOUR
AU  - Nikolić, Tatjana V.
AU  - Purać, Jelena
AU  - Orčić, Snežana
AU  - Kojić, Danijela
AU  - Vujanović, Dragana
AU  - Stanimirović, Zoran
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
AU  - Šikoparija, Branko
AU  - Blagojević, Duško P.
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2307
AB  - Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajaca, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.
PB  - Wiley-Blackwell, Hoboken
T2  - Archives of Insect Biochemistry and Physiology
T1  - Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee
VL  - 90
IS  - 4
SP  - 181
EP  - 194
DO  - 10.1002/arch.21253
ER  - 

@article{
author = "Nikolić, Tatjana V. and Purać, Jelena and Orčić, Snežana and Kojić, Danijela and Vujanović, Dragana and Stanimirović, Zoran and Gržetić, Ivan and Ilijević, Konstantin and Šikoparija, Branko and Blagojević, Duško P.",
year = "2015",
abstract = "Understanding the cellular stress response in honey bees will significantly contribute to their conservation. The aim of this study was to analyze the response of the antioxidative enzymes superoxide dismutase and catalase in honey bees related to the presence of toxic metals in different habitats. Three locations were selected: (i) Tunovo on the mountain Golija, as control area, without industry and large human impact, (ii) Belgrade as urban area, and (iii) Zajaca, as mining and industrial zone. Our results showed that the concentrations of lead (Pb) in whole body of bees vary according to habitat, but there was very significant increase of Pb in bees from investigated industrial area. Bees from urban and industrial area had increased expression of both Sod1 and Cat genes, suggesting adaptation to increased oxidative stress. However, in spite increased gene expression, the enzyme activity of catalase was lower in bees from industrial area suggesting inhibitory effect of Pb on catalase.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee",
volume = "90",
number = "4",
pages = "181-194",
doi = "10.1002/arch.21253"
}

Nikolić, T. V., Purać, J., Orčić, S., Kojić, D., Vujanović, D., Stanimirović, Z., Gržetić, I., Ilijević, K., Šikoparija, B.,& Blagojević, D. P.. (2015). Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee. in Archives of Insect Biochemistry and Physiology
Wiley-Blackwell, Hoboken., 90(4), 181-194.
https://doi.org/10.1002/arch.21253

Nikolić TV, Purać J, Orčić S, Kojić D, Vujanović D, Stanimirović Z, Gržetić I, Ilijević K, Šikoparija B, Blagojević DP. Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee. in Archives of Insect Biochemistry and Physiology. 2015;90(4):181-194.
doi:10.1002/arch.21253 .

Nikolić, Tatjana V., Purać, Jelena, Orčić, Snežana, Kojić, Danijela, Vujanović, Dragana, Stanimirović, Zoran, Gržetić, Ivan, Ilijević, Konstantin, Šikoparija, Branko, Blagojević, Duško P., "Environmental Effects on Superoxide Dismutase and Catalase Activity and Expression in Honey Bee" in Archives of Insect Biochemistry and Physiology, 90, no. 4 (2015):181-194,
https://doi.org/10.1002/arch.21253 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Blagojević, Duško
AU  - Šobajić, Slađana
AU  - Vasiljevska, Milijana
AU  - Poznanić, Milica
AU  - Radusinović, M.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1069
AB  - This study examined the influence of low, adequate and high dietary zinc intake on the growth and metabolic interactions between zinc, copper and iron in different Mongolian gerbil (Meriones unguiculatus) tissues. Animals were fed a basal diet (for 3 wk) containing a low-zinc level (8 mg/ kg diet, LZ-group), adequate zinc level (25 mg/kg diet, AZ-group) and saturated zinc level (38 mg/ kg diet, SZ-group). After comparing the body weight of gerbils, the present study demonstrated that the LZ-group (51.68 g +/- 3.35) showed growth retardation, contrary to the AZ- and SZ-groups (58.03 g +/- 2.18 and 62.35 g +/- 2.04, respectively). Concentrations of zinc in the liver, kidneys and testes in the LZ-group (41.28, 19.58, 22.55 mg/kg, respectively) were significantly lower than in the AZ-group (57.27, 23.73, 28.79 mg/kg, respectively) and the SZ-group (73.06, 30.07, 34.52 mg/kg, respectively). Results also showed that copper and iron concentrations in the kidney, heart and liver were significantly higher in the LZ-group (Cu 11.45, 7.01, 7.95 mg/kg; Fe 116.19, 126.07, 299.47 mg/kg) than in the AZ-group (Cu 8.72, 5.6, 6.5 mg/kg; Fe 97.27, 97.27, 250.25 mg/kg) and the SZ-group (Cu 6.48, 4.15, 4.8 mg/kg; Fe 74.95, 77.95, 200.27 mg/kg). There was an increase of testis copper concentration in the LZ-group (4.9 mg/kg) compared to the AZ- and SZ-groups (3.99 and 3.05 mg/kg, respectively), and there was no significant difference in testis iron concentration between animals in different diet groups. These results showed that a low-zinc diet had negative effects on growth and the concentration of zinc in the kidneys, liver and testes. This also affected homeostasis of copper and iron by increasing its distribution in the kidneys, liver and heart tissue. However, in testes only copper concentration was increased.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria Brno
T1  - Effects of low, adequate and high dietary zinc intake on metabolic interactions between zinc, copper and iron in different mongolian gerbil tissues
VL  - 77
IS  - 1
SP  - 17
EP  - 23
DO  - 10.2754/avb200877010017
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Blagojević, Duško and Šobajić, Slađana and Vasiljevska, Milijana and Poznanić, Milica and Radusinović, M.",
year = "2008",
abstract = "This study examined the influence of low, adequate and high dietary zinc intake on the growth and metabolic interactions between zinc, copper and iron in different Mongolian gerbil (Meriones unguiculatus) tissues. Animals were fed a basal diet (for 3 wk) containing a low-zinc level (8 mg/ kg diet, LZ-group), adequate zinc level (25 mg/kg diet, AZ-group) and saturated zinc level (38 mg/ kg diet, SZ-group). After comparing the body weight of gerbils, the present study demonstrated that the LZ-group (51.68 g +/- 3.35) showed growth retardation, contrary to the AZ- and SZ-groups (58.03 g +/- 2.18 and 62.35 g +/- 2.04, respectively). Concentrations of zinc in the liver, kidneys and testes in the LZ-group (41.28, 19.58, 22.55 mg/kg, respectively) were significantly lower than in the AZ-group (57.27, 23.73, 28.79 mg/kg, respectively) and the SZ-group (73.06, 30.07, 34.52 mg/kg, respectively). Results also showed that copper and iron concentrations in the kidney, heart and liver were significantly higher in the LZ-group (Cu 11.45, 7.01, 7.95 mg/kg; Fe 116.19, 126.07, 299.47 mg/kg) than in the AZ-group (Cu 8.72, 5.6, 6.5 mg/kg; Fe 97.27, 97.27, 250.25 mg/kg) and the SZ-group (Cu 6.48, 4.15, 4.8 mg/kg; Fe 74.95, 77.95, 200.27 mg/kg). There was an increase of testis copper concentration in the LZ-group (4.9 mg/kg) compared to the AZ- and SZ-groups (3.99 and 3.05 mg/kg, respectively), and there was no significant difference in testis iron concentration between animals in different diet groups. These results showed that a low-zinc diet had negative effects on growth and the concentration of zinc in the kidneys, liver and testes. This also affected homeostasis of copper and iron by increasing its distribution in the kidneys, liver and heart tissue. However, in testes only copper concentration was increased.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria Brno",
title = "Effects of low, adequate and high dietary zinc intake on metabolic interactions between zinc, copper and iron in different mongolian gerbil tissues",
volume = "77",
number = "1",
pages = "17-23",
doi = "10.2754/avb200877010017"
}

Cerović, A., Miletić, I., Blagojević, D., Šobajić, S., Vasiljevska, M., Poznanić, M.,& Radusinović, M.. (2008). Effects of low, adequate and high dietary zinc intake on metabolic interactions between zinc, copper and iron in different mongolian gerbil tissues. in Acta Veterinaria Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 77(1), 17-23.
https://doi.org/10.2754/avb200877010017

Cerović A, Miletić I, Blagojević D, Šobajić S, Vasiljevska M, Poznanić M, Radusinović M. Effects of low, adequate and high dietary zinc intake on metabolic interactions between zinc, copper and iron in different mongolian gerbil tissues. in Acta Veterinaria Brno. 2008;77(1):17-23.
doi:10.2754/avb200877010017 .

Cerović, Aleksandra, Miletić, Ivanka, Blagojević, Duško, Šobajić, Slađana, Vasiljevska, Milijana, Poznanić, Milica, Radusinović, M., "Effects of low, adequate and high dietary zinc intake on metabolic interactions between zinc, copper and iron in different mongolian gerbil tissues" in Acta Veterinaria Brno, 77, no. 1 (2008):17-23,
https://doi.org/10.2754/avb200877010017 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Šobajić, Slađana
AU  - Blagojević, Duško
AU  - Radusinović, Miodrag
AU  - El-Sohemy, Ahmed
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/927
AB  - Zinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.
PB  - Humana Press Inc, Totowa
T2  - Biological Trace Element Research
T1  - Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells
VL  - 116
IS  - 1
SP  - 61
EP  - 71
DO  - 10.1007/BF02685919
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Šobajić, Slađana and Blagojević, Duško and Radusinović, Miodrag and El-Sohemy, Ahmed",
year = "2007",
abstract = "Zinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.",
publisher = "Humana Press Inc, Totowa",
journal = "Biological Trace Element Research",
title = "Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells",
volume = "116",
number = "1",
pages = "61-71",
doi = "10.1007/BF02685919"
}

Cerović, A., Miletić, I., Šobajić, S., Blagojević, D., Radusinović, M.,& El-Sohemy, A.. (2007). Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells. in Biological Trace Element Research
Humana Press Inc, Totowa., 116(1), 61-71.
https://doi.org/10.1007/BF02685919

Cerović A, Miletić I, Šobajić S, Blagojević D, Radusinović M, El-Sohemy A. Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells. in Biological Trace Element Research. 2007;116(1):61-71.
doi:10.1007/BF02685919 .

Cerović, Aleksandra, Miletić, Ivanka, Šobajić, Slađana, Blagojević, Duško, Radusinović, Miodrag, El-Sohemy, Ahmed, "Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells" in Biological Trace Element Research, 116, no. 1 (2007):61-71,
https://doi.org/10.1007/BF02685919 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Šobajić, Slađana
AU  - Blagojević, Duško
AU  - Jones, David R.
AU  - Poznanić, Milica
AU  - Radusinović, Miodrag
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/707
AB  - The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin A on the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respectively). The following day, the animals were given sunflower oil containing 50 nmol vitamin A. The results showed that the concentration of zinc in blood plasma was similar in both groups. The amount of plasma chylomicrons was lower in the LZ group than in the SZ group (p  lt  0.001). The concentration of retinol in blood plasma was lower in the LZ group than in the SZ group (p  lt  0.01). However, the results demonstrated an increase in the blood plasma retinol concentration in the LZ group compared to the SZ group when calculated per milligram of plasma chylomicrons (p  lt  0.01). In plasma chylomicrons, fatty acids corresponding to 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0, and 20:4 were detected. The fatty acid distribution was similar in both groups. There was no major difference in the concentration of fatty acids in plasma chylomicrons between both experimental groups, except for 20:4 (a lower amount was found in the SZ group). Our results show that dietary zinc influences both the amount of chylomicrons in blood plasma and the concentrations of retinol and arachidonic acid in chylomicrons.
PB  - Humana Press Inc, Totowa
T2  - Biological Trace Element Research
T1  - Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons
VL  - 112
IS  - 2
SP  - 145
EP  - 158
DO  - 10.1385/BTER:112:2:145
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Šobajić, Slađana and Blagojević, Duško and Jones, David R. and Poznanić, Milica and Radusinović, Miodrag",
year = "2006",
abstract = "The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin A on the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respectively). The following day, the animals were given sunflower oil containing 50 nmol vitamin A. The results showed that the concentration of zinc in blood plasma was similar in both groups. The amount of plasma chylomicrons was lower in the LZ group than in the SZ group (p  lt  0.001). The concentration of retinol in blood plasma was lower in the LZ group than in the SZ group (p  lt  0.01). However, the results demonstrated an increase in the blood plasma retinol concentration in the LZ group compared to the SZ group when calculated per milligram of plasma chylomicrons (p  lt  0.01). In plasma chylomicrons, fatty acids corresponding to 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0, and 20:4 were detected. The fatty acid distribution was similar in both groups. There was no major difference in the concentration of fatty acids in plasma chylomicrons between both experimental groups, except for 20:4 (a lower amount was found in the SZ group). Our results show that dietary zinc influences both the amount of chylomicrons in blood plasma and the concentrations of retinol and arachidonic acid in chylomicrons.",
publisher = "Humana Press Inc, Totowa",
journal = "Biological Trace Element Research",
title = "Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons",
volume = "112",
number = "2",
pages = "145-158",
doi = "10.1385/BTER:112:2:145"
}

Cerović, A., Miletić, I., Šobajić, S., Blagojević, D., Jones, D. R., Poznanić, M.,& Radusinović, M.. (2006). Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons. in Biological Trace Element Research
Humana Press Inc, Totowa., 112(2), 145-158.
https://doi.org/10.1385/BTER:112:2:145

Cerović A, Miletić I, Šobajić S, Blagojević D, Jones DR, Poznanić M, Radusinović M. Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons. in Biological Trace Element Research. 2006;112(2):145-158.
doi:10.1385/BTER:112:2:145 .

Cerović, Aleksandra, Miletić, Ivanka, Šobajić, Slađana, Blagojević, Duško, Jones, David R., Poznanić, Milica, Radusinović, Miodrag, "Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons" in Biological Trace Element Research, 112, no. 2 (2006):145-158,
https://doi.org/10.1385/BTER:112:2:145 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Blagojević, Duško
AU  - Šobajić, Slađana
AU  - Čolić, Miodrag
AU  - Vasiljevska, Milijana
AU  - Poznanić, Milica
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/652
AB  - The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons.
AB  - Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Jugoslovenska medicinska biohemija
T1  - The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons
T1  - Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme
VL  - 24
IS  - 1
SP  - 15
EP  - 20
DO  - 10.2298/JMH0501015C
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Blagojević, Duško and Šobajić, Slađana and Čolić, Miodrag and Vasiljevska, Milijana and Poznanić, Milica",
year = "2005",
abstract = "The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons., Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska medicinska biohemija",
title = "The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons, Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme",
volume = "24",
number = "1",
pages = "15-20",
doi = "10.2298/JMH0501015C"
}

Cerović, A., Miletić, I., Blagojević, D., Šobajić, S., Čolić, M., Vasiljevska, M.,& Poznanić, M.. (2005). The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons. in Jugoslovenska medicinska biohemija
Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd., 24(1), 15-20.
https://doi.org/10.2298/JMH0501015C

Cerović A, Miletić I, Blagojević D, Šobajić S, Čolić M, Vasiljevska M, Poznanić M. The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons. in Jugoslovenska medicinska biohemija. 2005;24(1):15-20.
doi:10.2298/JMH0501015C .

Cerović, Aleksandra, Miletić, Ivanka, Blagojević, Duško, Šobajić, Slađana, Čolić, Miodrag, Vasiljevska, Milijana, Poznanić, Milica, "The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons" in Jugoslovenska medicinska biohemija, 24, no. 1 (2005):15-20,
https://doi.org/10.2298/JMH0501015C . .

TY  - CONF
AU  - Cerović, AM
AU  - Blagojević, Duško
AU  - Miletić, Ivanka
AU  - Šobajić, Slađana
AU  - Radusinović, MD
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/496
PB  - Federation Amer Soc Exp Biol, Bethesda
C3  - FASEB Journal
T1  - Distribution of blood plasma fatty acids related to zinc diet
VL  - 18
IS  - 5
UR  - https://hdl.handle.net/21.15107/rcub_farfar_496
ER  - 

@conference{
author = "Cerović, AM and Blagojević, Duško and Miletić, Ivanka and Šobajić, Slađana and Radusinović, MD",
year = "2004",
publisher = "Federation Amer Soc Exp Biol, Bethesda",
journal = "FASEB Journal",
title = "Distribution of blood plasma fatty acids related to zinc diet",
volume = "18",
number = "5",
url = "https://hdl.handle.net/21.15107/rcub_farfar_496"
}

Cerović, A., Blagojević, D., Miletić, I., Šobajić, S.,& Radusinović, M.. (2004). Distribution of blood plasma fatty acids related to zinc diet. in FASEB Journal
Federation Amer Soc Exp Biol, Bethesda., 18(5).
https://hdl.handle.net/21.15107/rcub_farfar_496

Cerović A, Blagojević D, Miletić I, Šobajić S, Radusinović M. Distribution of blood plasma fatty acids related to zinc diet. in FASEB Journal. 2004;18(5).
https://hdl.handle.net/21.15107/rcub_farfar_496 .

Cerović, AM, Blagojević, Duško, Miletić, Ivanka, Šobajić, Slađana, Radusinović, MD, "Distribution of blood plasma fatty acids related to zinc diet" in FASEB Journal, 18, no. 5 (2004),
https://hdl.handle.net/21.15107/rcub_farfar_496 .