TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Klisić, Aleksandra
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Memon, Lidija
AU  - Gaković, Branka
AU  - Bogavac-Stanojević, Nataša
AU  - Spasojević-Kalimanovska, Vesna
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4677
AB  - Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.
PB  - Termedia Publishing House Ltd.
T2  - Archives of Medical Science
T1  - Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress
VL  - 19
IS  - 2
SP  - 313
EP  - 323
DO  - 10.5114/aoms/136074
ER  - 

@article{
author = "Vukašinović, Aleksandra and Ostanek, Barbara and Klisić, Aleksandra and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Memon, Lidija and Gaković, Branka and Bogavac-Stanojević, Nataša and Spasojević-Kalimanovska, Vesna and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Introduction: Telomeres are protective chromosomal ends. Short telomeres are a proven biomarker of biological aging. We aimed to find an association of telomere length and telomerase activity in circulating leukocytes and thromboaspirates of patients with acute myocardial infarction. Furthermore, association of the telomere-telomerase system with oxidative stress markers (as common risk factors for coronary artery disease (CAD)) was tested. Material and methods: Patients were selected from the patients admitted to the intensive care unit with acute myocardial infarction with ST-segment elevation (STEMI), with the following inclusion criteria – STEMI patients between 18 and 80 years old of both genders and candidates for primary percutaneous coronary intervention, with infarction pain present for a maximum of 12 h. In all the patients leukocyte telomere length, telomerase activity and scores related to oxidative-stress status (Protective, Damage and OXY) were evaluated. Results: Patients were divided into different groups: with stable angina pectoris (AP) (n = 22), acute myocardial infarction with: STEMI (n = 93), non-obstructive coronary arteries (MINOCA) (n = 7), blood vessel rupture (n = 6) at three time points, and compared to the group of 84 healthy subjects. Telomerase activity was significantly higher in all CAD sub-groups compared to the control group (AP = 0.373 (0.355–0.386), STEMI = 0.375 (0.349–0.395), MINOCA = 0.391 (0.366–0.401), blood vessel rupture = 0.360 (0.352–0.385) vs. CG = 0.069 (0.061–0.081), p < 0.001), while telomeres were significantly shorter in STEMI, MINOCA and blood vessel rupture groups compared to the control group (STEMI = 1.179 (0.931–1.376), MINOCA = 1.026 (0.951–1.070), blood vessel rupture = 1.089 (0.842–1.173) vs. CG = 1.329 (1.096–1.624), p = 0.030]. Values of OXY score were significantly higher in STEMI and MINOCA patients compared to the control group and AP patients (5.83 (4.55–7.54) and 10.28 (9.19–10.72) vs. 4.94 (3.29–6.18) and 4.18 (2.58–4.86), p < 0.001). Longer telomeres and higher telomerase activity were found in thromboaspirates, compared to the peripheral blood leukocytes in the same patients (1.25 (1.01–1.84) vs. 1.18 (0.909–1.516), p = 0.036; and 0.366 (0.367–0.379) vs. 0.366 (0.367–0.379), p < 0.001, respectively). In addition, telomere length and telomerase activity had good diagnostic ability to separate STEMI patients from healthy persons. Conclusions: Leukocyte telomere length and telomerase activity can differentiate CAD patients from healthy persons, and relate CAD to oxidative stress.",
publisher = "Termedia Publishing House Ltd.",
journal = "Archives of Medical Science",
title = "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress",
volume = "19",
number = "2",
pages = "313-323",
doi = "10.5114/aoms/136074"
}

Vukašinović, A., Ostanek, B., Klisić, A., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Memon, L., Gaković, B., Bogavac-Stanojević, N., Spasojević-Kalimanovska, V., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science
Termedia Publishing House Ltd.., 19(2), 313-323.
https://doi.org/10.5114/aoms/136074

Vukašinović A, Ostanek B, Klisić A, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Memon L, Gaković B, Bogavac-Stanojević N, Spasojević-Kalimanovska V, Marc J, Nešković A, Kotur-Stevuljević J. Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress. in Archives of Medical Science. 2023;19(2):313-323.
doi:10.5114/aoms/136074 .

Vukašinović, Aleksandra, Ostanek, Barbara, Klisić, Aleksandra, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Memon, Lidija, Gaković, Branka, Bogavac-Stanojević, Nataša, Spasojević-Kalimanovska, Vesna, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Telomere-telomerase system status in patients with acute myocardial infarction with ST-segment elevation – relationship with oxidative stress" in Archives of Medical Science, 19, no. 2 (2023):313-323,
https://doi.org/10.5114/aoms/136074 . .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Klisic, Aleksandra
AU  - Ostanek, Barbara
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Sopić, Miron
AU  - Hinić, Saša
AU  - Stefanović, Milica
AU  - Bogavac-Stanojević, Nataša
AU  - Marc, Janja
AU  - Nešković, Aleksandar
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5078
AB  - In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?
VL  - 24
IS  - 18
DO  - 10.3390/ijms241814308
ER  - 

@article{
author = "Vukašinović, Aleksandra and Klisic, Aleksandra and Ostanek, Barbara and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Sopić, Miron and Hinić, Saša and Stefanović, Milica and Bogavac-Stanojević, Nataša and Marc, Janja and Nešković, Aleksandar and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "In the present study, we examined redox status parameters in arterial and venous blood samples, its potential to predict the prognosis of acute myocardial infarction (AMI) patients assessed through its impact on the comprehensive grading SYNTAX score, and its clinical accuracy. Potential connections between common blood biomarkers, biomarkers of redox status, leukocyte telomere length, and telomerase enzyme activity in the acute myocardial infarction burden were assessed using principal component analysis (PCA). This study included 92 patients with acute myocardial infarction. Significantly higher levels of advanced oxidation protein products (AOPP), superoxide anion (O2•−), ischemia-modified albumin (IMA), and significantly lower levels of total oxidant status (TOS) and total protein sulfhydryl (SH-) groups were found in arterial blood than in the peripheral venous blood samples, while biomarkers of the telomere–telomerase system did not show statistical significance in the two compared sample types (p = 0.834 and p = 0.419). To better understand the effect of the examined biomarkers in the AMI patients on SYNTAX score, those biomarkers were grouped using PCA, which merged them into the four the most contributing factors. The “cholesterol–protein factor” and “oxidative–telomere factor” were independent predictors of higher SYNTAX score (OR = 0.338, p = 0.008 and OR = 0.427, p = 0.035, respectively), while the ability to discriminate STEMI from non-STEMI patients had only the “oxidative–telomere factor” (AUC = 0.860, p = 0.008). The results show that traditional cardiovascular risk factors, i.e., high total cholesterol together with high total serum proteins and haemoglobin, are associated with severe disease progression in much the same way as a combination of redox biomarkers (pro-oxidant-antioxidant balance, total antioxidant status, IMA) and telomere length.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?",
volume = "24",
number = "18",
doi = "10.3390/ijms241814308"
}

Vukašinović, A., Klisic, A., Ostanek, B., Kafedžić, S., Zdravković, M., Ilić, I., Sopić, M., Hinić, S., Stefanović, M., Bogavac-Stanojević, N., Marc, J., Nešković, A.,& Kotur-Stevuljević, J.. (2023). Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences
MDPI., 24(18).
https://doi.org/10.3390/ijms241814308

Vukašinović A, Klisic A, Ostanek B, Kafedžić S, Zdravković M, Ilić I, Sopić M, Hinić S, Stefanović M, Bogavac-Stanojević N, Marc J, Nešković A, Kotur-Stevuljević J. Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?. in International Journal of Molecular Sciences. 2023;24(18).
doi:10.3390/ijms241814308 .

Vukašinović, Aleksandra, Klisic, Aleksandra, Ostanek, Barbara, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Sopić, Miron, Hinić, Saša, Stefanović, Milica, Bogavac-Stanojević, Nataša, Marc, Janja, Nešković, Aleksandar, Kotur-Stevuljević, Jelena, "Redox Status and Telomere–Telomerase System Biomarkers in Patients with Acute Myocardial Infarction Using a Principal Component Analysis: Is There a Link?" in International Journal of Molecular Sciences, 24, no. 18 (2023),
https://doi.org/10.3390/ijms241814308 . .

TY  - JOUR
AU  - Belić, Milica
AU  - Sopić, Miron
AU  - Roksandić-Milenković, Marina
AU  - Ćeriman, Vesna
AU  - Guzonjić, Azra
AU  - Vukašinović, Aleksandra
AU  - Ostanek, Barbara
AU  - Dimić, Nemanja
AU  - Jovanović, Dragana
AU  - Kotur-Stevuljević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5387
AB  - Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.
PB  - Charles University, Czech Republic
T2  - Folia biologica
T1  - Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis
VL  - 69
IS  - 2
SP  - 59
EP  - 68
DO  - 10.14712/fb2023069020059
ER  - 

@article{
author = "Belić, Milica and Sopić, Miron and Roksandić-Milenković, Marina and Ćeriman, Vesna and Guzonjić, Azra and Vukašinović, Aleksandra and Ostanek, Barbara and Dimić, Nemanja and Jovanović, Dragana and Kotur-Stevuljević, Jelena",
year = "2023",
abstract = "Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.",
publisher = "Charles University, Czech Republic",
journal = "Folia biologica",
title = "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis",
volume = "69",
number = "2",
pages = "59-68",
doi = "10.14712/fb2023069020059"
}

Belić, M., Sopić, M., Roksandić-Milenković, M., Ćeriman, V., Guzonjić, A., Vukašinović, A., Ostanek, B., Dimić, N., Jovanović, D.,& Kotur-Stevuljević, J.. (2023). Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica
Charles University, Czech Republic., 69(2), 59-68.
https://doi.org/10.14712/fb2023069020059

Belić M, Sopić M, Roksandić-Milenković M, Ćeriman V, Guzonjić A, Vukašinović A, Ostanek B, Dimić N, Jovanović D, Kotur-Stevuljević J. Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis. in Folia biologica. 2023;69(2):59-68.
doi:10.14712/fb2023069020059 .

Belić, Milica, Sopić, Miron, Roksandić-Milenković, Marina, Ćeriman, Vesna, Guzonjić, Azra, Vukašinović, Aleksandra, Ostanek, Barbara, Dimić, Nemanja, Jovanović, Dragana, Kotur-Stevuljević, Jelena, "Correlation of Short Leukocyte Telomeres and Oxidative
Stress with the Presence and Severity of Lung Cancer
Explored by Principal Component Analysis" in Folia biologica, 69, no. 2 (2023):59-68,
https://doi.org/10.14712/fb2023069020059 . .

TY  - JOUR
AU  - Guzonjić, Azra
AU  - Sopić, Miron
AU  - Ostanek, Barbara
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4185
AB  - As research related to healthspan and lifespan has become a hot topic, the necessity for a
reliable and practical biomarker of aging (BoA), which can provide information about mortality
and morbidity risk, along with remaining life expectancy, has increased. The chromosome
terminus non-coding protective structure that prevents genomic instability is called a telomere.
The continual shortening of telomeres, which affects their structure as well as function, is a
hallmark of agedness. The aforementioned process is a potential cause of age-related diseases
(ARDs), leading to a bad prognosis and a low survival rate, which compromise health and
longevity. Hence, studies scrutinizing the BoAs often include telomere length (TL) as a
prospective candidate. The results of these studies suggest that TL measurement can only provide
an approximate appraisal of the aging rate, and its implementation into clinical practice and
routine use as a BoA has many limitations and challenges. Nevertheless, measuring TL while
determining other biomarkers can be used to assess biological age. This review focuses on the
importance of telomeres in health, senescence, and diseases, as well as on summarizing the results
and conclusions of previous studies evaluating TL as a potential BoA.
AB  - Kako su istraživanja na temu starenja postala sve popularnija, javila se potreba za pouzdanim i praktičnim biomarkerom starenja, koji može pružiti informacije o riziku od mortaliteta i morbiditeta. Telomere su nekodirajući krajevi linearnih hromozoma koji održavaju njihovu stabilnost i integritet. Ćelijsko starenje i starenje organizma karakteriše progresivno skraćivanje telomera, što ugrožava njihovu strukturu i funkciju. Skraćivanje telomera je u vezi sa povećanom incidencom bolesti povezanih sa starenjem i lošom stopom preživljavanja, što ugrožava zdravlje i skraćuje životni vek. Stoga je dužina telomera dugo vremena prepoznata kao jedan od najboljih biomarkera starenja. Međutim, nedavna istraživanja ukazuju na to da dužina telomera može da pruži samo približnu procenu brzine starenja, pa je implementacija ovog biomarkera u kliničku praksu i rutinsku primenu praćena mnogim ograničenjima i izazovima. Uprkos tome, merenje dužine telomera, uz istovremeno određivanje drugih biomarkera, može poslužiti za procenu biološke starosti. Fokus ovog rada je na značaju telomera u ljudskom zdravlju, starenju i bolestima, kao i na sumiranju rezultata i zaključaka dosadašnjih studija koje su se bavile ispitivanjem dužine telomera kao potencijalnog kandidata za biomarker starenja.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Telomere length as a biomarker of aging and diseases
T1  - Dužina telomera kao biomarker starenja i bolesti
VL  - 72
IS  - 2
SP  - 105
EP  - 126
DO  - 10.5937/arhfarm72-36376
ER  - 

@article{
author = "Guzonjić, Azra and Sopić, Miron and Ostanek, Barbara and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "As research related to healthspan and lifespan has become a hot topic, the necessity for a
reliable and practical biomarker of aging (BoA), which can provide information about mortality
and morbidity risk, along with remaining life expectancy, has increased. The chromosome
terminus non-coding protective structure that prevents genomic instability is called a telomere.
The continual shortening of telomeres, which affects their structure as well as function, is a
hallmark of agedness. The aforementioned process is a potential cause of age-related diseases
(ARDs), leading to a bad prognosis and a low survival rate, which compromise health and
longevity. Hence, studies scrutinizing the BoAs often include telomere length (TL) as a
prospective candidate. The results of these studies suggest that TL measurement can only provide
an approximate appraisal of the aging rate, and its implementation into clinical practice and
routine use as a BoA has many limitations and challenges. Nevertheless, measuring TL while
determining other biomarkers can be used to assess biological age. This review focuses on the
importance of telomeres in health, senescence, and diseases, as well as on summarizing the results
and conclusions of previous studies evaluating TL as a potential BoA., Kako su istraživanja na temu starenja postala sve popularnija, javila se potreba za pouzdanim i praktičnim biomarkerom starenja, koji može pružiti informacije o riziku od mortaliteta i morbiditeta. Telomere su nekodirajući krajevi linearnih hromozoma koji održavaju njihovu stabilnost i integritet. Ćelijsko starenje i starenje organizma karakteriše progresivno skraćivanje telomera, što ugrožava njihovu strukturu i funkciju. Skraćivanje telomera je u vezi sa povećanom incidencom bolesti povezanih sa starenjem i lošom stopom preživljavanja, što ugrožava zdravlje i skraćuje životni vek. Stoga je dužina telomera dugo vremena prepoznata kao jedan od najboljih biomarkera starenja. Međutim, nedavna istraživanja ukazuju na to da dužina telomera može da pruži samo približnu procenu brzine starenja, pa je implementacija ovog biomarkera u kliničku praksu i rutinsku primenu praćena mnogim ograničenjima i izazovima. Uprkos tome, merenje dužine telomera, uz istovremeno određivanje drugih biomarkera, može poslužiti za procenu biološke starosti. Fokus ovog rada je na značaju telomera u ljudskom zdravlju, starenju i bolestima, kao i na sumiranju rezultata i zaključaka dosadašnjih studija koje su se bavile ispitivanjem dužine telomera kao potencijalnog kandidata za biomarker starenja.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Telomere length as a biomarker of aging and diseases, Dužina telomera kao biomarker starenja i bolesti",
volume = "72",
number = "2",
pages = "105-126",
doi = "10.5937/arhfarm72-36376"
}

Guzonjić, A., Sopić, M., Ostanek, B.,& Kotur-Stevuljević, J.. (2022). Telomere length as a biomarker of aging and diseases. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 72(2), 105-126.
https://doi.org/10.5937/arhfarm72-36376

Guzonjić A, Sopić M, Ostanek B, Kotur-Stevuljević J. Telomere length as a biomarker of aging and diseases. in Arhiv za farmaciju. 2022;72(2):105-126.
doi:10.5937/arhfarm72-36376 .

Guzonjić, Azra, Sopić, Miron, Ostanek, Barbara, Kotur-Stevuljević, Jelena, "Telomere length as a biomarker of aging and diseases" in Arhiv za farmaciju, 72, no. 2 (2022):105-126,
https://doi.org/10.5937/arhfarm72-36376 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Ninić, Ana
AU  - Ostanek, Barbara
AU  - Bojanin, Dragana
AU  - Milenković, Tatjana
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Vekić, Jelena
AU  - Marc, Janja
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4255
AB  - Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients.
AB  - Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus
T1  - Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om
VL  - 41
IS  - 3
SP  - 282
EP  - 289
DO  - 10.5937/jomb0-33220
ER  - 

@article{
author = "Sopić, Miron and Ninić, Ana and Ostanek, Barbara and Bojanin, Dragana and Milenković, Tatjana and Munjas, Jelena and Mihajlović, Marija and Vekić, Jelena and Marc, Janja and Spasojević-Kalimanovska, Vesna",
year = "2022",
abstract = "Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients., Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus, Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om",
volume = "41",
number = "3",
pages = "282-289",
doi = "10.5937/jomb0-33220"
}

Sopić, M., Ninić, A., Ostanek, B., Bojanin, D., Milenković, T., Munjas, J., Mihajlović, M., Vekić, J., Marc, J.,& Spasojević-Kalimanovska, V.. (2022). Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 41(3), 282-289.
https://doi.org/10.5937/jomb0-33220

Sopić M, Ninić A, Ostanek B, Bojanin D, Milenković T, Munjas J, Mihajlović M, Vekić J, Marc J, Spasojević-Kalimanovska V. Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry. 2022;41(3):282-289.
doi:10.5937/jomb0-33220 .

Sopić, Miron, Ninić, Ana, Ostanek, Barbara, Bojanin, Dragana, Milenković, Tatjana, Munjas, Jelena, Mihajlović, Marija, Vekić, Jelena, Marc, Janja, Spasojević-Kalimanovska, Vesna, "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus" in Journal of Medical Biochemistry, 41, no. 3 (2022):282-289,
https://doi.org/10.5937/jomb0-33220 . .

TY  - JOUR
AU  - Rauner, Martina
AU  - Foessl, Ines
AU  - Formosa, Melissa
AU  - Kague, Erika
AU  - Prijatelj, Vid
AU  - Alonso Lopez, Nerea
AU  - Banerjee, Bodhisattwa
AU  - Bergen, Dylan
AU  - Busse, Björn
AU  - Calado, Angelo
AU  - Douni, Eleni
AU  - Gabet, Yankel
AU  - Garcı´a Giralt, Natalia
AU  - Grinberg, Daniel
AU  - Lovsin, Nika
AU  - Nogues Solan, Xavier
AU  - Ostanek, Barbara
AU  - Pavlos, Nathan
AU  - Rivadeneira, Fernando
AU  - Soldatović, Ivan
AU  - van de Peppel, Jeroen
AU  - van der Eerden, Bram
AU  - van Hul, Wim
AU  - Balcells, Susanna
AU  - Marc, Janja
AU  - Reppe, Sjur
AU  - Søe, Kent
AU  - Karasik, David
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4019
AB  - The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques
VL  - 12
DO  - 10.3389/fendo.2021.731217
ER  - 

@article{
author = "Rauner, Martina and Foessl, Ines and Formosa, Melissa and Kague, Erika and Prijatelj, Vid and Alonso Lopez, Nerea and Banerjee, Bodhisattwa and Bergen, Dylan and Busse, Björn and Calado, Angelo and Douni, Eleni and Gabet, Yankel and Garcı´a Giralt, Natalia and Grinberg, Daniel and Lovsin, Nika and Nogues Solan, Xavier and Ostanek, Barbara and Pavlos, Nathan and Rivadeneira, Fernando and Soldatović, Ivan and van de Peppel, Jeroen and van der Eerden, Bram and van Hul, Wim and Balcells, Susanna and Marc, Janja and Reppe, Sjur and Søe, Kent and Karasik, David",
year = "2021",
abstract = "The availability of large human datasets for genome-wide association studies (GWAS) and the advancement of sequencing technologies have boosted the identification of genetic variants in complex and rare diseases in the skeletal field. Yet, interpreting results from human association studies remains a challenge. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary. Multiple unknowns exist for putative causal genes, including cellular localization of the molecular function. Intermediate traits (“endophenotypes”), e.g. molecular quantitative trait loci (molQTLs), are needed to identify mechanisms of underlying associations. Furthermore, index variants often reside in non-coding regions of the genome, therefore challenging for interpretation. Knowledge of non-coding variance (e.g. ncRNAs), repetitive sequences, and regulatory interactions between enhancers and their target genes is central for understanding causal genes in skeletal conditions. Animal models with deep skeletal phenotyping and cell culture models have already facilitated fine mapping of some association signals, elucidated gene mechanisms, and revealed disease-relevant biology. However, to accelerate research towards bridging the current gap between association and causality in skeletal diseases, alternative in vivo platforms need to be used and developed in parallel with the current -omics and traditional in vivo resources. Therefore, we argue that as a field we need to establish resource-sharing standards to collectively address complex research questions. These standards will promote data integration from various -omics technologies and functional dissection of human complex traits. In this mission statement, we review the current available resources and as a group propose a consensus to facilitate resource sharing using existing and future resources. Such coordination efforts will maximize the acquisition of knowledge from different approaches and thus reduce redundancy and duplication of resources. These measures will help to understand the pathogenesis of osteoporosis and other skeletal diseases towards defining new and more efficient therapeutic targets.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques",
volume = "12",
doi = "10.3389/fendo.2021.731217"
}

Rauner, M., Foessl, I., Formosa, M., Kague, E., Prijatelj, V., Alonso Lopez, N., Banerjee, B., Bergen, D., Busse, B., Calado, A., Douni, E., Gabet, Y., Garcı´a Giralt, N., Grinberg, D., Lovsin, N., Nogues Solan, X., Ostanek, B., Pavlos, N., Rivadeneira, F., Soldatović, I., van de Peppel, J., van der Eerden, B., van Hul, W., Balcells, S., Marc, J., Reppe, S., Søe, K.,& Karasik, D.. (2021). Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology
Frontiers Media S.A.., 12.
https://doi.org/10.3389/fendo.2021.731217

Rauner M, Foessl I, Formosa M, Kague E, Prijatelj V, Alonso Lopez N, Banerjee B, Bergen D, Busse B, Calado A, Douni E, Gabet Y, Garcı´a Giralt N, Grinberg D, Lovsin N, Nogues Solan X, Ostanek B, Pavlos N, Rivadeneira F, Soldatović I, van de Peppel J, van der Eerden B, van Hul W, Balcells S, Marc J, Reppe S, Søe K, Karasik D. Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques. in Frontiers in Endocrinology. 2021;12.
doi:10.3389/fendo.2021.731217 .

Rauner, Martina, Foessl, Ines, Formosa, Melissa, Kague, Erika, Prijatelj, Vid, Alonso Lopez, Nerea, Banerjee, Bodhisattwa, Bergen, Dylan, Busse, Björn, Calado, Angelo, Douni, Eleni, Gabet, Yankel, Garcı´a Giralt, Natalia, Grinberg, Daniel, Lovsin, Nika, Nogues Solan, Xavier, Ostanek, Barbara, Pavlos, Nathan, Rivadeneira, Fernando, Soldatović, Ivan, van de Peppel, Jeroen, van der Eerden, Bram, van Hul, Wim, Balcells, Susanna, Marc, Janja, Reppe, Sjur, Søe, Kent, Karasik, David, "Perspective of the GEMSTONE Consortium on Current and Future Approaches to Functional Validation for Skeletal Genetic Disease Using Cellular, Molecular and Animal-Modeling Techniques" in Frontiers in Endocrinology, 12 (2021),
https://doi.org/10.3389/fendo.2021.731217 . .

TY  - CONF
AU  - Ilić, Tijana
AU  - Vidović, Bojana
AU  - Kotur-Stevuljević, Jelena
AU  - Ostanek, Barbara
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5518
AB  - Telomeres are DNA-protein structures located at the ends of eukaryotic chromosomes. Since
their shortening is a natural process, which repeats with each cell division, telomere lengths
have been proposed as a biomarker of aging. Also, there is evidence that telomere shortening
and dysfunction can be accelerated by oxidative-stress and unhealthy lifestyle factors,
including physical inactivity and inadequate nutrition, Thus, we aimed to evaluate the
association of redox status and selected lifestyle factors with telomere length. The study
included 94 apparently healthy adults, both genders with average age 46±12 years. Before
anthropometric measurements and venous blood sampling, participants were asked to complete
a lifestyle questionnaire. Serum antioxidant defense markers (total sulfhydryl groups,
paraoxonase activity and total antioxidant status) and prooxidants and products of its activity
(malondialdehyde, superoxide anion and total oxidant status) were determined. The
Prooxidative score, Antioxidative score and Oxy score were calculated from measured redox
status markers by using z-score statistics. Telomere length was determined by qPCR method
using genomic DNA from peripheral blood leukocytes. A positive relation was found among
telomere length and a moderate level of physical activity, intake of fruits and vegetables,
especially for females. Increasing abdominal fat, alcohol and fried food intake, as well the Oxy
score (difference between Proxidative and Antioxidative scores) were inversely associated with
telomere length. Overall, these results support the impact of healthy lifestyle on healthy aging
and redox control in the term of prevention of major age-related diseases.
PB  - University of Belgrade, Belgrade, Serbia
C3  - Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade
T1  - Association of redox status and lifestyle factors with telomere length in healthy subjects
SP  - 38
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5518
ER  - 

@conference{
author = "Ilić, Tijana and Vidović, Bojana and Kotur-Stevuljević, Jelena and Ostanek, Barbara",
year = "2020",
abstract = "Telomeres are DNA-protein structures located at the ends of eukaryotic chromosomes. Since
their shortening is a natural process, which repeats with each cell division, telomere lengths
have been proposed as a biomarker of aging. Also, there is evidence that telomere shortening
and dysfunction can be accelerated by oxidative-stress and unhealthy lifestyle factors,
including physical inactivity and inadequate nutrition, Thus, we aimed to evaluate the
association of redox status and selected lifestyle factors with telomere length. The study
included 94 apparently healthy adults, both genders with average age 46±12 years. Before
anthropometric measurements and venous blood sampling, participants were asked to complete
a lifestyle questionnaire. Serum antioxidant defense markers (total sulfhydryl groups,
paraoxonase activity and total antioxidant status) and prooxidants and products of its activity
(malondialdehyde, superoxide anion and total oxidant status) were determined. The
Prooxidative score, Antioxidative score and Oxy score were calculated from measured redox
status markers by using z-score statistics. Telomere length was determined by qPCR method
using genomic DNA from peripheral blood leukocytes. A positive relation was found among
telomere length and a moderate level of physical activity, intake of fruits and vegetables,
especially for females. Increasing abdominal fat, alcohol and fried food intake, as well the Oxy
score (difference between Proxidative and Antioxidative scores) were inversely associated with
telomere length. Overall, these results support the impact of healthy lifestyle on healthy aging
and redox control in the term of prevention of major age-related diseases.",
publisher = "University of Belgrade, Belgrade, Serbia",
journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade",
title = "Association of redox status and lifestyle factors with telomere length in healthy subjects",
pages = "38-38",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5518"
}

Ilić, T., Vidović, B., Kotur-Stevuljević, J.,& Ostanek, B.. (2020). Association of redox status and lifestyle factors with telomere length in healthy subjects. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade
University of Belgrade, Belgrade, Serbia., 38-38.
https://hdl.handle.net/21.15107/rcub_farfar_5518

Ilić T, Vidović B, Kotur-Stevuljević J, Ostanek B. Association of redox status and lifestyle factors with telomere length in healthy subjects. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade. 2020;:38-38.
https://hdl.handle.net/21.15107/rcub_farfar_5518 .

Ilić, Tijana, Vidović, Bojana, Kotur-Stevuljević, Jelena, Ostanek, Barbara, "Association of redox status and lifestyle factors with telomere length in healthy subjects" in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade (2020):38-38,
https://hdl.handle.net/21.15107/rcub_farfar_5518 .

TY  - JOUR
AU  - Vukašinović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Mlakar, Vid
AU  - Sopić, Miron
AU  - Cvetković, Zorica P.
AU  - Petković, Miloš
AU  - Spasojević-Kalimanovska, Vesna
AU  - Bogavac-Stanojević, Nataša
AU  - Ostanek, Barbara
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3356
AB  - Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p  lt  .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p  lt  .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Telomerase stability and evaluation of real-time telomeric repeat amplification protocol
VL  - 79
IS  - 3
SP  - 188
EP  - 193
DO  - 10.1080/00365513.2019.1576220
ER  - 

@article{
author = "Vukašinović, Aleksandra and Kotur-Stevuljević, Jelena and Mlakar, Vid and Sopić, Miron and Cvetković, Zorica P. and Petković, Miloš and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Ostanek, Barbara",
year = "2019",
abstract = "Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p  lt  .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p  lt  .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Telomerase stability and evaluation of real-time telomeric repeat amplification protocol",
volume = "79",
number = "3",
pages = "188-193",
doi = "10.1080/00365513.2019.1576220"
}

Vukašinović, A., Kotur-Stevuljević, J., Mlakar, V., Sopić, M., Cvetković, Z. P., Petković, M., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N.,& Ostanek, B.. (2019). Telomerase stability and evaluation of real-time telomeric repeat amplification protocol. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 79(3), 188-193.
https://doi.org/10.1080/00365513.2019.1576220

Vukašinović A, Kotur-Stevuljević J, Mlakar V, Sopić M, Cvetković ZP, Petković M, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Ostanek B. Telomerase stability and evaluation of real-time telomeric repeat amplification protocol. in Scandinavian Journal of Clinical and Laboratory Investigation. 2019;79(3):188-193.
doi:10.1080/00365513.2019.1576220 .

Vukašinović, Aleksandra, Kotur-Stevuljević, Jelena, Mlakar, Vid, Sopić, Miron, Cvetković, Zorica P., Petković, Miloš, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Ostanek, Barbara, "Telomerase stability and evaluation of real-time telomeric repeat amplification protocol" in Scandinavian Journal of Clinical and Laboratory Investigation, 79, no. 3 (2019):188-193,
https://doi.org/10.1080/00365513.2019.1576220 . .