TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Jovanović-Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5613
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Jovanović-Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka-Aleksić, M., Jovanović-Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka-Aleksić M, Jovanović-Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Jovanović-Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - JOUR
AU  - Selaković, Snežana
AU  - Rodić, Marko
AU  - Novaković, Irena
AU  - Matić, Ivana
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Milčić, Vesna
AU  - Dimiza, Filitsa
AU  - Psomas, George
AU  - Anđelković, Katarina
AU  - Šumar-Ristović, Maja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5522
AB  - Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.
PB  - Royal Society of Chemistry
T2  - Dalton Transactions
T1  - Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach
VL  - 53
IS  - 6
SP  - 2770
EP  - 2788
DO  - 10.1039/d3dt03862a
ER  - 

@article{
author = "Selaković, Snežana and Rodić, Marko and Novaković, Irena and Matić, Ivana and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Milčić, Vesna and Dimiza, Filitsa and Psomas, George and Anđelković, Katarina and Šumar-Ristović, Maja",
year = "2024",
abstract = "Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.",
publisher = "Royal Society of Chemistry",
journal = "Dalton Transactions",
title = "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach",
volume = "53",
number = "6",
pages = "2770-2788",
doi = "10.1039/d3dt03862a"
}

Selaković, S., Rodić, M., Novaković, I., Matić, I., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Milčić, V., Dimiza, F., Psomas, G., Anđelković, K.,& Šumar-Ristović, M.. (2024). Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions
Royal Society of Chemistry., 53(6), 2770-2788.
https://doi.org/10.1039/d3dt03862a

Selaković S, Rodić M, Novaković I, Matić I, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Milčić V, Dimiza F, Psomas G, Anđelković K, Šumar-Ristović M. Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions. 2024;53(6):2770-2788.
doi:10.1039/d3dt03862a .

Selaković, Snežana, Rodić, Marko, Novaković, Irena, Matić, Ivana, Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Milčić, Vesna, Dimiza, Filitsa, Psomas, George, Anđelković, Katarina, Šumar-Ristović, Maja, "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach" in Dalton Transactions, 53, no. 6 (2024):2770-2788,
https://doi.org/10.1039/d3dt03862a . .

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Marković, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4747
AB  - Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Prospective study of cytotoxic and genotoxic effects of Combretastatin
SP  - 133
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4747
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Topalović, Dijana and Živković, Lada and Marković, Milica and Pirković, Andrea",
year = "2023",
abstract = "Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Prospective study of cytotoxic and genotoxic effects of Combretastatin",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4747"
}

Spremo-Potparević, B., Topalović, D., Živković, L., Marković, M.,& Pirković, A.. (2023). Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747

Spremo-Potparević B, Topalović D, Živković L, Marković M, Pirković A. Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747 .

Spremo-Potparević, Biljana, Topalović, Dijana, Živković, Lada, Marković, Milica, Pirković, Andrea, "Prospective study of cytotoxic and genotoxic effects of Combretastatin" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):133-133,
https://hdl.handle.net/21.15107/rcub_farfar_4747 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4919
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 

@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}

Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J. M.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis
Oxford University Press., 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013

Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković JM. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .

Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan M., "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .

TY  - JOUR
AU  - Krstić, Sanja
AU  - Miljić, Milorad
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5034
AB  - Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.
PB  - Elsevier Ltd
T2  - Food Chemistry Advances
T1  - Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity
VL  - 3
DO  - 10.1016/j.focha.2023.100395
ER  - 

@article{
author = "Krstić, Sanja and Miljić, Milorad and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Pirković, Andrea",
year = "2023",
abstract = "Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry Advances",
title = "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity",
volume = "3",
doi = "10.1016/j.focha.2023.100395"
}

Krstić, S., Miljić, M., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Pirković, A.. (2023). Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances
Elsevier Ltd., 3.
https://doi.org/10.1016/j.focha.2023.100395

Krstić S, Miljić M, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Pirković A. Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances. 2023;3.
doi:10.1016/j.focha.2023.100395 .

Krstić, Sanja, Miljić, Milorad, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Pirković, Andrea, "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity" in Food Chemistry Advances, 3 (2023),
https://doi.org/10.1016/j.focha.2023.100395 . .

TY  - JOUR
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Pirković, Andrea
AU  - Periša, Jovana
AU  - Murafa, Natalija
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Zarubica, Aleksandra
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4281
AB  - The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control
PB  - MDPI
T2  - Nanomaterials
T1  - Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin
VL  - 12
IS  - 18
DO  - 10.3390/nano12183195
ER  - 

@article{
author = "Sredojević, Dušan and Lazić, Vesna and Pirković, Andrea and Periša, Jovana and Murafa, Natalija and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Zarubica, Aleksandra and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2022",
abstract = "The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control",
publisher = "MDPI",
journal = "Nanomaterials",
title = "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin",
volume = "12",
number = "18",
doi = "10.3390/nano12183195"
}

Sredojević, D., Lazić, V., Pirković, A., Periša, J., Murafa, N., Spremo-Potparević, B., Živković, L., Topalović, D., Zarubica, A., Jovanović Krivokuća, M.,& Nedeljković, J.. (2022). Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials
MDPI., 12(18).
https://doi.org/10.3390/nano12183195

Sredojević D, Lazić V, Pirković A, Periša J, Murafa N, Spremo-Potparević B, Živković L, Topalović D, Zarubica A, Jovanović Krivokuća M, Nedeljković J. Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials. 2022;12(18).
doi:10.3390/nano12183195 .

Sredojević, Dušan, Lazić, Vesna, Pirković, Andrea, Periša, Jovana, Murafa, Natalija, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Zarubica, Aleksandra, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin" in Nanomaterials, 12, no. 18 (2022),
https://doi.org/10.3390/nano12183195 . .

TY  - JOUR
AU  - Vitomirov, Teodora
AU  - Dimiza, Filitsa
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Novaković, Irena
AU  - Anđelković, Katarina
AU  - Milčić, Miloš
AU  - Psomas, George
AU  - Šumar Ristović, Maja
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4248
AB  - In this article, cytotoxicity, the mechanisms of cytotoxic activity, genotoxicity, and interaction with DNA and proteins, of two Cu(II) complexes with a salicylaldehyde derivative (4-(diethylamino)salicylaldehyde) and α-diimine (2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen)) are reported. Both Cu(II) complexes performed cytotoxic effects against all tested malignant cell lines. Complexes exerted highest cytotoxicity against HeLa and A375 malignant cell lines. The cytotoxic activity of Cu(II) complex with phen as a α-diimine co-ligand was significantly higher in comparison with cytotoxic activity of Cu(II) complex with bipy. Pretreatment with specific inhibitors of caspase-3, caspase-8 or caspase-9, in order to clear up the mode of cell death triggered by two Cu(II) complexes in HeLa cells, indicated the ability of these complexes to induce apoptosis through activation of target caspases. Cu(II)-phen complex exhibited significant antioxidant activity compared with Cu(II)-bipy complex, and showed a better effect on reducing intracellular ROS levels in HeLa cells. Tested complexes did not display genotoxic potential in human peripheral blood leucocytes, but exhibited an antigenotoxic effect in post-treatment, after H2O2 exposure. The study of the in vitro biological properties regarding their affinity towards CT (calf-thymus) DNA and serum albumins showed that the compounds can intercalate to CT DNA, and bind reversibly and tightly to the albumins. Molecular docking studies of the ability of compounds to bind to biomacromolecules are consistent with in vitro studies.
PB  - Elsevier Inc.
T2  - Journal of Inorganic Biochemistry
T1  - Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins
VL  - 235
DO  - 10.1016/j.jinorgbio.2022.111942
ER  - 

@article{
author = "Vitomirov, Teodora and Dimiza, Filitsa and Matić, Ivana Z. and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Novaković, Irena and Anđelković, Katarina and Milčić, Miloš and Psomas, George and Šumar Ristović, Maja",
year = "2022",
abstract = "In this article, cytotoxicity, the mechanisms of cytotoxic activity, genotoxicity, and interaction with DNA and proteins, of two Cu(II) complexes with a salicylaldehyde derivative (4-(diethylamino)salicylaldehyde) and α-diimine (2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen)) are reported. Both Cu(II) complexes performed cytotoxic effects against all tested malignant cell lines. Complexes exerted highest cytotoxicity against HeLa and A375 malignant cell lines. The cytotoxic activity of Cu(II) complex with phen as a α-diimine co-ligand was significantly higher in comparison with cytotoxic activity of Cu(II) complex with bipy. Pretreatment with specific inhibitors of caspase-3, caspase-8 or caspase-9, in order to clear up the mode of cell death triggered by two Cu(II) complexes in HeLa cells, indicated the ability of these complexes to induce apoptosis through activation of target caspases. Cu(II)-phen complex exhibited significant antioxidant activity compared with Cu(II)-bipy complex, and showed a better effect on reducing intracellular ROS levels in HeLa cells. Tested complexes did not display genotoxic potential in human peripheral blood leucocytes, but exhibited an antigenotoxic effect in post-treatment, after H2O2 exposure. The study of the in vitro biological properties regarding their affinity towards CT (calf-thymus) DNA and serum albumins showed that the compounds can intercalate to CT DNA, and bind reversibly and tightly to the albumins. Molecular docking studies of the ability of compounds to bind to biomacromolecules are consistent with in vitro studies.",
publisher = "Elsevier Inc.",
journal = "Journal of Inorganic Biochemistry",
title = "Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins",
volume = "235",
doi = "10.1016/j.jinorgbio.2022.111942"
}

Vitomirov, T., Dimiza, F., Matić, I. Z., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Novaković, I., Anđelković, K., Milčić, M., Psomas, G.,& Šumar Ristović, M.. (2022). Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins. in Journal of Inorganic Biochemistry
Elsevier Inc.., 235.
https://doi.org/10.1016/j.jinorgbio.2022.111942

Vitomirov T, Dimiza F, Matić IZ, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Novaković I, Anđelković K, Milčić M, Psomas G, Šumar Ristović M. Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins. in Journal of Inorganic Biochemistry. 2022;235.
doi:10.1016/j.jinorgbio.2022.111942 .

Vitomirov, Teodora, Dimiza, Filitsa, Matić, Ivana Z., Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Novaković, Irena, Anđelković, Katarina, Milčić, Miloš, Psomas, George, Šumar Ristović, Maja, "Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins" in Journal of Inorganic Biochemistry, 235 (2022),
https://doi.org/10.1016/j.jinorgbio.2022.111942 . .

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Dimitrijević Srećković, Vesna
AU  - Pirković, Andrea
AU  - Petrović, Hristina
AU  - Spremo-Potparević, Biljana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4630
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro
SP  - 94
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4630
ER  - 

@conference{
author = "Živković, Lada and Topalović, Dijana and Borozan, Sunčica and Bajić, Vladan and Dimitrijević Srećković, Vesna and Pirković, Andrea and Petrović, Hristina and Spremo-Potparević, Biljana",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro",
pages = "94-94",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4630"
}

Živković, L., Topalović, D., Borozan, S., Bajić, V., Dimitrijević Srećković, V., Pirković, A., Petrović, H.,& Spremo-Potparević, B.. (2022). Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630

Živković L, Topalović D, Borozan S, Bajić V, Dimitrijević Srećković V, Pirković A, Petrović H, Spremo-Potparević B. Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

Živković, Lada, Topalović, Dijana, Borozan, Sunčica, Bajić, Vladan, Dimitrijević Srećković, Vesna, Pirković, Andrea, Petrović, Hristina, Spremo-Potparević, Biljana, "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):94-94,
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

TY  - CHAP
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5184
AB  - Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.
PB  - Elsevier
T2  - Olives and Olive Oil in Health and Disease Prevention
T1  - Olive leaf, DNA damage and chelation therapy
SP  - 457
EP  - 469
DO  - 10.1016/B978-0-12-819528-4.00021-3
ER  - 

@inbook{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.",
publisher = "Elsevier",
journal = "Olives and Olive Oil in Health and Disease Prevention",
booktitle = "Olive leaf, DNA damage and chelation therapy",
pages = "457-469",
doi = "10.1016/B978-0-12-819528-4.00021-3"
}

Čabarkapa-Pirković, A., Živković, L., Dekanski, D., Topalović, D.,& Spremo-Potparević, B.. (2021). Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention
Elsevier., 457-469.
https://doi.org/10.1016/B978-0-12-819528-4.00021-3

Čabarkapa-Pirković A, Živković L, Dekanski D, Topalović D, Spremo-Potparević B. Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention. 2021;:457-469.
doi:10.1016/B978-0-12-819528-4.00021-3 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Spremo-Potparević, Biljana, "Olive leaf, DNA damage and chelation therapy" in Olives and Olive Oil in Health and Disease Prevention (2021):457-469,
https://doi.org/10.1016/B978-0-12-819528-4.00021-3 . .

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920
AB  - Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
AB  - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
T1  - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
VL  - 71
IS  - 3
SP  - 197
EP  - 206
DO  - 10.5937/arhfarm71‐31060
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada",
year = "2021",
abstract = "Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka",
volume = "71",
number = "3",
pages = "197-206",
doi = "10.5937/arhfarm71‐31060"
}

Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206.
https://doi.org/10.5937/arhfarm71‐31060

Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206.
doi:10.5937/arhfarm71‐31060 .

Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206,
https://doi.org/10.5937/arhfarm71‐31060 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes-Hernández, Tamara
AU  - Zlatković-Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
PB  - Wiley
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.",
publisher = "Wiley",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry
Wiley., 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Potparević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3092
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Dekanski, Dragana and Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V., Dekanski, D., Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić V, Dekanski D, Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan, Dekanski, Dragana, Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - CONF
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Žukovec, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5186
AB  - Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5186
ER  - 

@conference{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Žukovec, Dijana and Dekanski, Dragana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Salvianolic acid B (Sal B) is a polyphenol compound derived from the Salvia miltiorrhiza plant which showed multitude of biological activities in vitro, including antioxidant, anti-inflammatory, cytotoxic and neuroprotective properties. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5186"
}

Čabarkapa-Pirković, A., Živković, L., Žukovec, D., Dekanski, D., Bajić, V.,& Spremo-Potparević, B.. (2017). Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186

Čabarkapa-Pirković A, Živković L, Žukovec D, Dekanski D, Bajić V, Spremo-Potparević B. Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

Čabarkapa-Pirković, Andrea, Živković, Lada, Žukovec, Dijana, Dekanski, Dragana, Bajić, Vladan, Spremo-Potparević, Biljana, "Salvianolic acid B reduces hydrogen peroxide induced DNA damage in human peripheral blood cells in vitro" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):110-110,
https://hdl.handle.net/21.15107/rcub_farfar_5186 .

TY  - CONF
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Borozan, Sunčica
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5127
AB  - Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...
PB  - International Comet Assay Working Group (ICAWG)
C3  - 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
T1  - Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5127
ER  - 

@conference{
author = "Topalović, Dijana and Živković, Lada and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Bajić, Vladan and Borozan, Sunčica and Spremo-Potparević, Biljana",
year = "2017",
abstract = "Long-term exposure to higher levels of estrogens and estrogen-like compounds has been associated with the range of adverse effects including development of breast, ovarian or endometrial cancer. It is well known that estrogen-like compounds mediate DNA damage by generation of reactive oxygen species (ROS), implying that their effects can be modulated by antioxidants. ...",
publisher = "International Comet Assay Working Group (ICAWG)",
journal = "12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain",
title = "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5127"
}

Topalović, D., Živković, L., Čabarkapa-Pirković, A., Dekanski, D., Bajić, V., Borozan, S.,& Spremo-Potparević, B.. (2017). Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain
International Comet Assay Working Group (ICAWG)., 58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127

Topalović D, Živković L, Čabarkapa-Pirković A, Dekanski D, Bajić V, Borozan S, Spremo-Potparević B. Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay. in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain. 2017;:58-58.
https://hdl.handle.net/21.15107/rcub_farfar_5127 .

Topalović, Dijana, Živković, Lada, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Bajić, Vladan, Borozan, Sunčica, Spremo-Potparević, Biljana, "Antigenotoxic potential of dry olive leaf extract against DNA damage induced by estradiol and diethylstilbestrol on human peripheral blood leucocytes in Comet assay" in 12th International Comet Assay Workshop (ICAW 2017), 29–31 August, 2017, University of Navarra, Pamplona, Spain (2017):58-58,
https://hdl.handle.net/21.15107/rcub_farfar_5127 .

TY  - JOUR
AU  - Janković, Milena
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2941
AB  - Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role.
AB  - Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro
T1  - Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro
VL  - 51
IS  - 2
SP  - 39
EP  - 45
DO  - 10.5937/mckg51-15559
ER  - 

@article{
author = "Janković, Milena and Živković, Lada and Pirković, Andrea and Topalović, Dijana and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role., Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro, Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro",
volume = "51",
number = "2",
pages = "39-45",
doi = "10.5937/mckg51-15559"
}

Janković, M., Živković, L., Pirković, A., Topalović, D., Dekanski, D., Bajić, V.,& Potparević, B.. (2017). Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 51(2), 39-45.
https://doi.org/10.5937/mckg51-15559

Janković M, Živković L, Pirković A, Topalović D, Dekanski D, Bajić V, Potparević B. Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis. 2017;51(2):39-45.
doi:10.5937/mckg51-15559 .

Janković, Milena, Živković, Lada, Pirković, Andrea, Topalović, Dijana, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro" in Medicinski časopis, 51, no. 2 (2017):39-45,
https://doi.org/10.5937/mckg51-15559 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2822
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 

@article{
author = "Pirković, Andrea and Dekanski, Dragana and Živković, Lada and Milanović-Čabarkapa, Mirjana and Bajić, Vladan and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Potparević, Biljana",
year = "2017",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}

Pirković, A., Dekanski, D., Živković, L., Milanović-Čabarkapa, M., Bajić, V., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023

Pirković A, Dekanski D, Živković L, Milanović-Čabarkapa M, Bajić V, Topalović D, Giampieri F, Gasparrini M, Battino M, Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623.
doi:10.1016/j.fct.2016.12.023 .

Pirković, Andrea, Dekanski, Dragana, Živković, Lada, Milanović-Čabarkapa, Mirjana, Bajić, Vladan, Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Kotur-Stevuljević, Jelena
AU  - Isenović, Esma
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana
AU  - Lazić, Vesna
AU  - Ahrenkiel, S. Phillip
AU  - Nedeljković, Jovan M.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2968
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.
PB  - Elsevier Science BV, Amsterdam
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 

@article{
author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Pirković, Andrea and Kotur-Stevuljević, Jelena and Isenović, Esma and Sredojević, Dušan and Vukoje, Ivana and Lazić, Vesna and Ahrenkiel, S. Phillip and Nedeljković, Jovan M.",
year = "2017",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}

Bajić, V., Potparević, B., Živković, L., Pirković, A., Kotur-Stevuljević, J., Isenović, E., Sredojević, D., Vukoje, I., Lazić, V., Ahrenkiel, S. P.,& Nedeljković, J. M.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces
Elsevier Science BV, Amsterdam., 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032

Bajić V, Potparević B, Živković L, Pirković A, Kotur-Stevuljević J, Isenović E, Sredojević D, Vukoje I, Lazić V, Ahrenkiel SP, Nedeljković JM. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces. 2017;155:323-331.
doi:10.1016/j.colsurfb.2017.04.032 .

Bajić, Vladan, Potparević, Biljana, Živković, Lada, Pirković, Andrea, Kotur-Stevuljević, Jelena, Isenović, Esma, Sredojević, Dušan, Vukoje, Ivana, Lazić, Vesna, Ahrenkiel, S. Phillip, Nedeljković, Jovan M., "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2906
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Pirković, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S., Pirković, A., Topalović, D., Ciptasari, U., Bajić, V.,& Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2017/8759764

Živković L, Borozan S, Pirković A, Topalović D, Ciptasari U, Bajić V, Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica, Pirković, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan, Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 . .

TY  - CONF
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Vukoje, Ivana
AU  - Kotur-Stevuljević, Jelena
AU  - Nedeljković, Jovan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5296
AB  - Ascorbic acid (AA) is a standard antioxidant and its radical scavenging properties also appear to be responsible for its antigenotoxic properties. Nanoparticle-mediated delivery of antioxidant compounds is intended to increase their bioavailability while maintaining their effectiveness.  Colloids consisting of the 45-Å TiO2 nanoparticles (NPs) with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color.  The aim of this study was to compare, for the first time, the antioxidant and antigenotoxic effects of AA attached to the surface of TiO2 NPs with free AA in a wide concentration range. For evaluation of antigenotoxic properties whole blood cells were first treated with 50 μM H2O2 to induce DNA damage, and then exposed to 3 different concentrations of free AA (1.3×10-2, 2.6×10-2, and 3.9×10-2 M) and the same concentrations of AA attached to TiO2 NPs (0.05, 0.1, and 0.15 M) for 30 min at 37 0C. The level of DNA damage was evaluated by comet assay method. For evaluation of antioxidant properties, total antioxidant capacity (TAC), total antioxidative status (TAS) and prooxidative-antioxidative balance (PAB) were determined in human serum pool during 2 and 24 h incubation at 37 °C, without and with terc-buthyl-hydroperoxide (TBH) as exogenously added oxidant. As expected, the results of DNA damage showed that the increase of AA concentration leads to a reduction of DNA damage. The similar concentration dependence was observed for surface-modified TiO2 NPs with AA. So, no significant differences between the antigenotoxic properties of free AA and AA attached to the TiO2 NPs were noticed, but only the highest concentrations showed significant effect in both experimental treatments. Regarding short-term oxidative balance in biological material (serum), during 2h, by measuring the TAC we have showed that the complex NP and AA, so as ascorbic acid showed a significant increase in TAC capacity, compared to native serum. This increase of antioxidative capacity couldn’t be abrogated even with a powerful oxidant, terc-buthylhydroperoxide presence (TBH). After 24h hour incubation the TAC level in both samples decreased towards the baseline level. About the TOS, which measures all oxidative components in plasma such as hydrogen-peroxide and lipid hydroperoxide, the complex of NP and AA versus AA alone showed inconsistent results. Prooxidative-antioxidative balance (PAB) measuring equilibrium between oxidants and antioxidants remains low, almost imponderable after 2h and after 24h serum incubation with the two substances. To summarize, we suggest that surface-modified TiO2 NPs with AA and/or similar compounds can be used to improve their bioavailability while maintaining its beneficial activities.
PB  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016
C3  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage
SP  - 51
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5296
ER  - 

@conference{
author = "Bajić, Vladan and Spremo-Potparević, Biljana and Živković, Lada and Čabarkapa, Andrea and Vukoje, Ivana and Kotur-Stevuljević, Jelena and Nedeljković, Jovan",
year = "2016",
abstract = "Ascorbic acid (AA) is a standard antioxidant and its radical scavenging properties also appear to be responsible for its antigenotoxic properties. Nanoparticle-mediated delivery of antioxidant compounds is intended to increase their bioavailability while maintaining their effectiveness.  Colloids consisting of the 45-Å TiO2 nanoparticles (NPs) with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color.  The aim of this study was to compare, for the first time, the antioxidant and antigenotoxic effects of AA attached to the surface of TiO2 NPs with free AA in a wide concentration range. For evaluation of antigenotoxic properties whole blood cells were first treated with 50 μM H2O2 to induce DNA damage, and then exposed to 3 different concentrations of free AA (1.3×10-2, 2.6×10-2, and 3.9×10-2 M) and the same concentrations of AA attached to TiO2 NPs (0.05, 0.1, and 0.15 M) for 30 min at 37 0C. The level of DNA damage was evaluated by comet assay method. For evaluation of antioxidant properties, total antioxidant capacity (TAC), total antioxidative status (TAS) and prooxidative-antioxidative balance (PAB) were determined in human serum pool during 2 and 24 h incubation at 37 °C, without and with terc-buthyl-hydroperoxide (TBH) as exogenously added oxidant. As expected, the results of DNA damage showed that the increase of AA concentration leads to a reduction of DNA damage. The similar concentration dependence was observed for surface-modified TiO2 NPs with AA. So, no significant differences between the antigenotoxic properties of free AA and AA attached to the TiO2 NPs were noticed, but only the highest concentrations showed significant effect in both experimental treatments. Regarding short-term oxidative balance in biological material (serum), during 2h, by measuring the TAC we have showed that the complex NP and AA, so as ascorbic acid showed a significant increase in TAC capacity, compared to native serum. This increase of antioxidative capacity couldn’t be abrogated even with a powerful oxidant, terc-buthylhydroperoxide presence (TBH). After 24h hour incubation the TAC level in both samples decreased towards the baseline level. About the TOS, which measures all oxidative components in plasma such as hydrogen-peroxide and lipid hydroperoxide, the complex of NP and AA versus AA alone showed inconsistent results. Prooxidative-antioxidative balance (PAB) measuring equilibrium between oxidants and antioxidants remains low, almost imponderable after 2h and after 24h serum incubation with the two substances. To summarize, we suggest that surface-modified TiO2 NPs with AA and/or similar compounds can be used to improve their bioavailability while maintaining its beneficial activities.",
publisher = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016",
journal = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage",
pages = "51-51",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5296"
}

Bajić, V., Spremo-Potparević, B., Živković, L., Čabarkapa, A., Vukoje, I., Kotur-Stevuljević, J.,& Nedeljković, J.. (2016). Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book
International Congress on Occupational & Environmental Toxicology - ICOETox 2016., 51-51.
https://hdl.handle.net/21.15107/rcub_farfar_5296

Bajić V, Spremo-Potparević B, Živković L, Čabarkapa A, Vukoje I, Kotur-Stevuljević J, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book. 2016;:51-51.
https://hdl.handle.net/21.15107/rcub_farfar_5296 .

Bajić, Vladan, Spremo-Potparević, Biljana, Živković, Lada, Čabarkapa, Andrea, Vukoje, Ivana, Kotur-Stevuljević, Jelena, Nedeljković, Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage" in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book (2016):51-51,
https://hdl.handle.net/21.15107/rcub_farfar_5296 .

TY  - CONF
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5231
AB  - The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
PB  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016
C3  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book
T1  - Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5231
ER  - 

@conference{
author = "Čabarkapa, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.",
publisher = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016",
journal = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book",
title = "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5231"
}

Čabarkapa, A., Živković, L., Dekanski, D., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2016). Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book
International Congress on Occupational & Environmental Toxicology - ICOETox 2016., 135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5231

Čabarkapa A, Živković L, Dekanski D, Topalović D, Bajić V, Spremo-Potparević B. Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book. 2016;:135-135.
https://hdl.handle.net/21.15107/rcub_farfar_5231 .

Čabarkapa, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers" in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book (2016):135-135,
https://hdl.handle.net/21.15107/rcub_farfar_5231 .

TY  - JOUR
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Bajić, Vladan
AU  - Jovičić, Snežana
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2538
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 

@article{
author = "Živković, Lada and Pirković, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan and Jovičić, Snežana and Potparević, Biljana",
year = "2016",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}

Živković, L., Pirković, A., Marčetić, M., Kovačević, N., Bajić, V., Jovičić, S.,& Potparević, B.. (2016). Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z

Živković L, Pirković A, Marčetić M, Kovačević N, Bajić V, Jovičić S, Potparević B. Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144.
doi:10.2298/ABS150512135Z .

Živković, Lada, Pirković, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan, Jovičić, Snežana, Potparević, Biljana, "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z . .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2635
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
PB  - Innovision Communications, Aliso Viejo
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1311
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana",
year = "2016",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
publisher = "Innovision Communications, Aliso Viejo",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1311"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V., Đelić, N.,& Potparević, B.. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine
Innovision Communications, Aliso Viejo., 22, 24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311

Vasiljević J, Živković L, Čabarkapa A, Bajić V, Đelić N, Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Zlatković-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jančić, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2584
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright
PB  - Wiley-Blackwell, Hoboken
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Pirković, Andrea and Živković, Lada and Borozan, Sunčica and Zlatković-Svenda, Mirjana and Dekanski, Dragana and Jančić, Ivan and Radak-Perović, Marija and Bajić, Vladan and Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Pirković, A., Živković, L., Borozan, S., Zlatković-Svenda, M., Dekanski, D., Jančić, I., Radak-Perović, M., Bajić, V.,& Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research
Wiley-Blackwell, Hoboken., 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Pirković A, Živković L, Borozan S, Zlatković-Svenda M, Dekanski D, Jančić I, Radak-Perović M, Bajić V, Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Pirković, Andrea, Živković, Lada, Borozan, Sunčica, Zlatković-Svenda, Mirjana, Dekanski, Dragana, Jančić, Ivan, Radak-Perović, Marija, Bajić, Vladan, Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .