TY  - JOUR
AU  - Milicević, Zorka
AU  - Kasapović, Jelena
AU  - Gavrilović, Ljubica
AU  - Milovanović, Zorka
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2096
AB  - It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.
PB  - Excli Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Mutant p53 protein expression and antioxidant status deficiency in breast cancer
VL  - 13
SP  - 691
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_vinar_113
ER  - 

@article{
author = "Milicević, Zorka and Kasapović, Jelena and Gavrilović, Ljubica and Milovanović, Zorka and Bajić, Vladan and Potparević, Biljana",
year = "2014",
abstract = "It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.",
publisher = "Excli Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Mutant p53 protein expression and antioxidant status deficiency in breast cancer",
volume = "13",
pages = "691-708",
url = "https://hdl.handle.net/21.15107/rcub_vinar_113"
}

Milicević, Z., Kasapović, J., Gavrilović, L., Milovanović, Z., Bajić, V.,& Potparević, B.. (2014). Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal
Excli Journal Managing Office, Dortmund., 13, 691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113

Milicević Z, Kasapović J, Gavrilović L, Milovanović Z, Bajić V, Potparević B. Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal. 2014;13:691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113 .

Milicević, Zorka, Kasapović, Jelena, Gavrilović, Ljubica, Milovanović, Zorka, Bajić, Vladan, Potparević, Biljana, "Mutant p53 protein expression and antioxidant status deficiency in breast cancer" in EXCLI Journal, 13 (2014):691-708,
https://hdl.handle.net/21.15107/rcub_vinar_113 .