TY  - JOUR
AU  - Gligorović-Barhanović, Najdana
AU  - Antunović, Tanja
AU  - Kavarić, Sreten
AU  - Đogo, Aleksandar
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3321
AB  - Background: Laboratory thyroid function tests play a central role in the diagnosis of thyroid disorders. The aim of our cross-sectional study was to determine reference values for thyroid tests in a rigorously selected group of Montenegrin females, investigate the impact of possible age-related changes and the influence of the interassay bias between three frequently used immunoassays. Methods: Female subjects were randomly selected, aged between 20 and 69 and 946 of them met the selection criteria. TSH, fT3, fT4, thyroid peroxidase and thyroglobulin antibodies were measured. Eighty samples were further analyzed on two other immunochemistry platforms. Results: Median TSH progressively increased with age, there was no difference in fT3, while fT4 was significantly higher in the two oldest groups compared to the others. When using the age-related 97.5 percentile of TSH the percentage of reclassification was highest in the 20-29 years of age group (5.2%, p lt 0.05). In the oldest band, 7.7% had TSH values above cohort-specific and below the age-related upper reference limit. Bland-Altman bias plots revealed the highest interassay absolute mean difference between compared TSH assays of 24.5% and for fT4 assays of 13.8%. Conclusions: The correlation coefficients between fT3 assays from different manufacturers were low. Serum TSH and fT4 concentrations increased with age and the implementation of age-specific TSH reference intervals would be of interest. The bias between the three commercial immunoassays indicated that the standardization of thyroid function tests is a task of great importance.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Age and assay related changes of laboratory thyroid function tests in the reference female population
VL  - 38
IS  - 1
SP  - 22
EP  - 32
DO  - 10.2478/jomb-2018-0020
ER  - 

@article{
author = "Gligorović-Barhanović, Najdana and Antunović, Tanja and Kavarić, Sreten and Đogo, Aleksandar and Spasojević-Kalimanovska, Vesna",
year = "2019",
abstract = "Background: Laboratory thyroid function tests play a central role in the diagnosis of thyroid disorders. The aim of our cross-sectional study was to determine reference values for thyroid tests in a rigorously selected group of Montenegrin females, investigate the impact of possible age-related changes and the influence of the interassay bias between three frequently used immunoassays. Methods: Female subjects were randomly selected, aged between 20 and 69 and 946 of them met the selection criteria. TSH, fT3, fT4, thyroid peroxidase and thyroglobulin antibodies were measured. Eighty samples were further analyzed on two other immunochemistry platforms. Results: Median TSH progressively increased with age, there was no difference in fT3, while fT4 was significantly higher in the two oldest groups compared to the others. When using the age-related 97.5 percentile of TSH the percentage of reclassification was highest in the 20-29 years of age group (5.2%, p lt 0.05). In the oldest band, 7.7% had TSH values above cohort-specific and below the age-related upper reference limit. Bland-Altman bias plots revealed the highest interassay absolute mean difference between compared TSH assays of 24.5% and for fT4 assays of 13.8%. Conclusions: The correlation coefficients between fT3 assays from different manufacturers were low. Serum TSH and fT4 concentrations increased with age and the implementation of age-specific TSH reference intervals would be of interest. The bias between the three commercial immunoassays indicated that the standardization of thyroid function tests is a task of great importance.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Age and assay related changes of laboratory thyroid function tests in the reference female population",
volume = "38",
number = "1",
pages = "22-32",
doi = "10.2478/jomb-2018-0020"
}

Gligorović-Barhanović, N., Antunović, T., Kavarić, S., Đogo, A.,& Spasojević-Kalimanovska, V.. (2019). Age and assay related changes of laboratory thyroid function tests in the reference female population. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 38(1), 22-32.
https://doi.org/10.2478/jomb-2018-0020

Gligorović-Barhanović N, Antunović T, Kavarić S, Đogo A, Spasojević-Kalimanovska V. Age and assay related changes of laboratory thyroid function tests in the reference female population. in Journal of Medical Biochemistry. 2019;38(1):22-32.
doi:10.2478/jomb-2018-0020 .

Gligorović-Barhanović, Najdana, Antunović, Tanja, Kavarić, Sreten, Đogo, Aleksandar, Spasojević-Kalimanovska, Vesna, "Age and assay related changes of laboratory thyroid function tests in the reference female population" in Journal of Medical Biochemistry, 38, no. 1 (2019):22-32,
https://doi.org/10.2478/jomb-2018-0020 . .

TY  - JOUR
AU  - Pejović, M.
AU  - Bogavac-Stanojević, Nataša
AU  - Popović, D.
AU  - Kavarić, Sreten
AU  - Stojanov, Marina
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1759
AB  - Context. There is a lack of evidence On thyroid hormonal status and the prevalence of thyroid autoimmune diseases in Montenegro. Objective. In order to get an idea about that we performed thyroid function tests on ambulatory patients from different areas of Montenegro. Design. The study took place since November 2005 till March 2007 at the Clinical Center of Montenegro. Subjects and methods. The study included 277 subjects of both sexes, referred for serum thyroid-stimulating hormone (TSH), thyroxin (T4) and free thyroxin (FT4), thyroperoxidase antibody (TPOAb) and thyroglobulin antibody (TgAb). Also a multi-step statistical analysis of the biochemical data was performed. Results. An age-dependent increment in serum TSH was detected in both males (p = 0.001) and females (p = 0.034). In females significant changes of T4 with age were detected (p = 0.017), but not in males (p = 0.427). Neither in males nor in females changes in FT4 were found (p = 0.342 and p = 0.831, respectively). A univariate logistic model demonstrated an association between thyroid antibodies and TSH, indicating that TSH >= 7.15 mU/L. was a better predictor of TPOAb (p = 0.001) than of TgAb (p = 0.01) presence. A multivariate model adjusted for both age and gender gave similar results. The highest TSH increment (86%) was found in sera containing both antibodies, while 33% of antibodies negative persons had TSH above 7.16 mU/L. Conclusion. The results demonstrate high percentage of subclinical thyroiditis among the investigated subjects.
PB  - Editura Acad Romane, Bucuresti
T2  - Acta Endocrinologica-Bucharest
T1  - A pilot study of thyroid testing in Montenegro
VL  - 8
IS  - 2
SP  - 309
EP  - 319
DO  - 10.4183/aeb.2012.309
ER  - 

@article{
author = "Pejović, M. and Bogavac-Stanojević, Nataša and Popović, D. and Kavarić, Sreten and Stojanov, Marina",
year = "2012",
abstract = "Context. There is a lack of evidence On thyroid hormonal status and the prevalence of thyroid autoimmune diseases in Montenegro. Objective. In order to get an idea about that we performed thyroid function tests on ambulatory patients from different areas of Montenegro. Design. The study took place since November 2005 till March 2007 at the Clinical Center of Montenegro. Subjects and methods. The study included 277 subjects of both sexes, referred for serum thyroid-stimulating hormone (TSH), thyroxin (T4) and free thyroxin (FT4), thyroperoxidase antibody (TPOAb) and thyroglobulin antibody (TgAb). Also a multi-step statistical analysis of the biochemical data was performed. Results. An age-dependent increment in serum TSH was detected in both males (p = 0.001) and females (p = 0.034). In females significant changes of T4 with age were detected (p = 0.017), but not in males (p = 0.427). Neither in males nor in females changes in FT4 were found (p = 0.342 and p = 0.831, respectively). A univariate logistic model demonstrated an association between thyroid antibodies and TSH, indicating that TSH >= 7.15 mU/L. was a better predictor of TPOAb (p = 0.001) than of TgAb (p = 0.01) presence. A multivariate model adjusted for both age and gender gave similar results. The highest TSH increment (86%) was found in sera containing both antibodies, while 33% of antibodies negative persons had TSH above 7.16 mU/L. Conclusion. The results demonstrate high percentage of subclinical thyroiditis among the investigated subjects.",
publisher = "Editura Acad Romane, Bucuresti",
journal = "Acta Endocrinologica-Bucharest",
title = "A pilot study of thyroid testing in Montenegro",
volume = "8",
number = "2",
pages = "309-319",
doi = "10.4183/aeb.2012.309"
}

Pejović, M., Bogavac-Stanojević, N., Popović, D., Kavarić, S.,& Stojanov, M.. (2012). A pilot study of thyroid testing in Montenegro. in Acta Endocrinologica-Bucharest
Editura Acad Romane, Bucuresti., 8(2), 309-319.
https://doi.org/10.4183/aeb.2012.309

Pejović M, Bogavac-Stanojević N, Popović D, Kavarić S, Stojanov M. A pilot study of thyroid testing in Montenegro. in Acta Endocrinologica-Bucharest. 2012;8(2):309-319.
doi:10.4183/aeb.2012.309 .

Pejović, M., Bogavac-Stanojević, Nataša, Popović, D., Kavarić, Sreten, Stojanov, Marina, "A pilot study of thyroid testing in Montenegro" in Acta Endocrinologica-Bucharest, 8, no. 2 (2012):309-319,
https://doi.org/10.4183/aeb.2012.309 . .