TY  - JOUR
AU  - Jovanović, Dragana
AU  - Milenković, Marina
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Jelena
AU  - Ceriman, Vesna
AU  - Kontić, Milica
AU  - Skodrić-Trifunović, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3136
AB  - Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.
PB  - Ame Publ Co, Shatin
T2  - Journal of Thoracic Disease
T1  - Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study
VL  - 10
IS  - 12
SP  - 6660
EP  - 6669
DO  - 10.21037/jtd.2018.11.16
ER  - 

@article{
author = "Jovanović, Dragana and Milenković, Marina and Kotur-Stevuljević, Jelena and Marković, Jelena and Ceriman, Vesna and Kontić, Milica and Skodrić-Trifunović, Vesna",
year = "2018",
abstract = "Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.",
publisher = "Ame Publ Co, Shatin",
journal = "Journal of Thoracic Disease",
title = "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study",
volume = "10",
number = "12",
pages = "6660-6669",
doi = "10.21037/jtd.2018.11.16"
}

Jovanović, D., Milenković, M., Kotur-Stevuljević, J., Marković, J., Ceriman, V., Kontić, M.,& Skodrić-Trifunović, V.. (2018). Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease
Ame Publ Co, Shatin., 10(12), 6660-6669.
https://doi.org/10.21037/jtd.2018.11.16

Jovanović D, Milenković M, Kotur-Stevuljević J, Marković J, Ceriman V, Kontić M, Skodrić-Trifunović V. Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease. 2018;10(12):6660-6669.
doi:10.21037/jtd.2018.11.16 .

Jovanović, Dragana, Milenković, Marina, Kotur-Stevuljević, Jelena, Marković, Jelena, Ceriman, Vesna, Kontić, Milica, Skodrić-Trifunović, Vesna, "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study" in Journal of Thoracic Disease, 10, no. 12 (2018):6660-6669,
https://doi.org/10.21037/jtd.2018.11.16 . .

TY  - CONF
AU  - Jovanović, Dragana M.
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Jelena
AU  - Loncarević, Olivera
AU  - Simon, Marioara
AU  - Milenković, Marina
AU  - Vukanić, Ivana
AU  - Kontić, Milica
AU  - Samardžić, Natalija
AU  - Ceriman, Vesna
AU  - Popević, Spasoje
AU  - Glumac, Sofija
AU  - Skodrić-Trifunović, Vesna
AU  - Radojević-Skodrić, Sanja
AU  - Ilić, Branislav
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3133
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research
VL  - 52
DO  - 10.1183/13993003.congress-2018.PA4232
ER  - 

@conference{
author = "Jovanović, Dragana M. and Kotur-Stevuljević, Jelena and Marković, Jelena and Loncarević, Olivera and Simon, Marioara and Milenković, Marina and Vukanić, Ivana and Kontić, Milica and Samardžić, Natalija and Ceriman, Vesna and Popević, Spasoje and Glumac, Sofija and Skodrić-Trifunović, Vesna and Radojević-Skodrić, Sanja and Ilić, Branislav",
year = "2018",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research",
volume = "52",
doi = "10.1183/13993003.congress-2018.PA4232"
}

Jovanović, D. M., Kotur-Stevuljević, J., Marković, J., Loncarević, O., Simon, M., Milenković, M., Vukanić, I., Kontić, M., Samardžić, N., Ceriman, V., Popević, S., Glumac, S., Skodrić-Trifunović, V., Radojević-Skodrić, S.,& Ilić, B.. (2018). PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 52.
https://doi.org/10.1183/13993003.congress-2018.PA4232

Jovanović DM, Kotur-Stevuljević J, Marković J, Loncarević O, Simon M, Milenković M, Vukanić I, Kontić M, Samardžić N, Ceriman V, Popević S, Glumac S, Skodrić-Trifunović V, Radojević-Skodrić S, Ilić B. PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research. in European Respiratory Journal. 2018;52.
doi:10.1183/13993003.congress-2018.PA4232 .

Jovanović, Dragana M., Kotur-Stevuljević, Jelena, Marković, Jelena, Loncarević, Olivera, Simon, Marioara, Milenković, Marina, Vukanić, Ivana, Kontić, Milica, Samardžić, Natalija, Ceriman, Vesna, Popević, Spasoje, Glumac, Sofija, Skodrić-Trifunović, Vesna, Radojević-Skodrić, Sanja, Ilić, Branislav, "PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research" in European Respiratory Journal, 52 (2018),
https://doi.org/10.1183/13993003.congress-2018.PA4232 . .

TY  - CONF
AU  - Jovanović, Dragana M.
AU  - Kotur-Stevuljević, Jelena
AU  - Roksandić-Milenković, Marina
AU  - Simon, Marioara
AU  - Ceriman, Vesna
AU  - Popević, Spasoje
AU  - Vukanić, Ivana
AU  - Samardžić, Natalija
AU  - Kontić, Milica
AU  - Ilić, Branislav
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3119
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - Soluble sPD-L1 plasma concentrations in a series of 95 lung cancer patients
VL  - 52
DO  - 10.1183/13993003.congress-2018.PA2218
ER  - 

@conference{
author = "Jovanović, Dragana M. and Kotur-Stevuljević, Jelena and Roksandić-Milenković, Marina and Simon, Marioara and Ceriman, Vesna and Popević, Spasoje and Vukanić, Ivana and Samardžić, Natalija and Kontić, Milica and Ilić, Branislav",
year = "2018",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Soluble sPD-L1 plasma concentrations in a series of 95 lung cancer patients",
volume = "52",
doi = "10.1183/13993003.congress-2018.PA2218"
}

Jovanović, D. M., Kotur-Stevuljević, J., Roksandić-Milenković, M., Simon, M., Ceriman, V., Popević, S., Vukanić, I., Samardžić, N., Kontić, M.,& Ilić, B.. (2018). Soluble sPD-L1 plasma concentrations in a series of 95 lung cancer patients. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 52.
https://doi.org/10.1183/13993003.congress-2018.PA2218

Jovanović DM, Kotur-Stevuljević J, Roksandić-Milenković M, Simon M, Ceriman V, Popević S, Vukanić I, Samardžić N, Kontić M, Ilić B. Soluble sPD-L1 plasma concentrations in a series of 95 lung cancer patients. in European Respiratory Journal. 2018;52.
doi:10.1183/13993003.congress-2018.PA2218 .

Jovanović, Dragana M., Kotur-Stevuljević, Jelena, Roksandić-Milenković, Marina, Simon, Marioara, Ceriman, Vesna, Popević, Spasoje, Vukanić, Ivana, Samardžić, Natalija, Kontić, Milica, Ilić, Branislav, "Soluble sPD-L1 plasma concentrations in a series of 95 lung cancer patients" in European Respiratory Journal, 52 (2018),
https://doi.org/10.1183/13993003.congress-2018.PA2218 . .