TY  - JOUR
AU  - Jovanović, Dragana
AU  - Milenković, Marina
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Jelena
AU  - Ceriman, Vesna
AU  - Kontić, Milica
AU  - Skodrić-Trifunović, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3136
AB  - Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.
PB  - Ame Publ Co, Shatin
T2  - Journal of Thoracic Disease
T1  - Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study
VL  - 10
IS  - 12
SP  - 6660
EP  - 6669
DO  - 10.21037/jtd.2018.11.16
ER  - 

@article{
author = "Jovanović, Dragana and Milenković, Marina and Kotur-Stevuljević, Jelena and Marković, Jelena and Ceriman, Vesna and Kontić, Milica and Skodrić-Trifunović, Vesna",
year = "2018",
abstract = "Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.",
publisher = "Ame Publ Co, Shatin",
journal = "Journal of Thoracic Disease",
title = "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study",
volume = "10",
number = "12",
pages = "6660-6669",
doi = "10.21037/jtd.2018.11.16"
}

Jovanović, D., Milenković, M., Kotur-Stevuljević, J., Marković, J., Ceriman, V., Kontić, M.,& Skodrić-Trifunović, V.. (2018). Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease
Ame Publ Co, Shatin., 10(12), 6660-6669.
https://doi.org/10.21037/jtd.2018.11.16

Jovanović D, Milenković M, Kotur-Stevuljević J, Marković J, Ceriman V, Kontić M, Skodrić-Trifunović V. Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease. 2018;10(12):6660-6669.
doi:10.21037/jtd.2018.11.16 .

Jovanović, Dragana, Milenković, Marina, Kotur-Stevuljević, Jelena, Marković, Jelena, Ceriman, Vesna, Kontić, Milica, Skodrić-Trifunović, Vesna, "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study" in Journal of Thoracic Disease, 10, no. 12 (2018):6660-6669,
https://doi.org/10.21037/jtd.2018.11.16 . .

TY  - CONF
AU  - Jovanović, Dragana M.
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Jelena
AU  - Loncarević, Olivera
AU  - Simon, Marioara
AU  - Milenković, Marina
AU  - Vukanić, Ivana
AU  - Kontić, Milica
AU  - Samardžić, Natalija
AU  - Ceriman, Vesna
AU  - Popević, Spasoje
AU  - Glumac, Sofija
AU  - Skodrić-Trifunović, Vesna
AU  - Radojević-Skodrić, Sanja
AU  - Ilić, Branislav
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3133
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research
VL  - 52
DO  - 10.1183/13993003.congress-2018.PA4232
ER  - 

@conference{
author = "Jovanović, Dragana M. and Kotur-Stevuljević, Jelena and Marković, Jelena and Loncarević, Olivera and Simon, Marioara and Milenković, Marina and Vukanić, Ivana and Kontić, Milica and Samardžić, Natalija and Ceriman, Vesna and Popević, Spasoje and Glumac, Sofija and Skodrić-Trifunović, Vesna and Radojević-Skodrić, Sanja and Ilić, Branislav",
year = "2018",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research",
volume = "52",
doi = "10.1183/13993003.congress-2018.PA4232"
}

Jovanović, D. M., Kotur-Stevuljević, J., Marković, J., Loncarević, O., Simon, M., Milenković, M., Vukanić, I., Kontić, M., Samardžić, N., Ceriman, V., Popević, S., Glumac, S., Skodrić-Trifunović, V., Radojević-Skodrić, S.,& Ilić, B.. (2018). PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 52.
https://doi.org/10.1183/13993003.congress-2018.PA4232

Jovanović DM, Kotur-Stevuljević J, Marković J, Loncarević O, Simon M, Milenković M, Vukanić I, Kontić M, Samardžić N, Ceriman V, Popević S, Glumac S, Skodrić-Trifunović V, Radojević-Skodrić S, Ilić B. PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research. in European Respiratory Journal. 2018;52.
doi:10.1183/13993003.congress-2018.PA4232 .

Jovanović, Dragana M., Kotur-Stevuljević, Jelena, Marković, Jelena, Loncarević, Olivera, Simon, Marioara, Milenković, Marina, Vukanić, Ivana, Kontić, Milica, Samardžić, Natalija, Ceriman, Vesna, Popević, Spasoje, Glumac, Sofija, Skodrić-Trifunović, Vesna, Radojević-Skodrić, Sanja, Ilić, Branislav, "PD-L1 expression on alveolar macrophages and in plasma of IPF patients - original research" in European Respiratory Journal, 52 (2018),
https://doi.org/10.1183/13993003.congress-2018.PA4232 . .

TY  - JOUR
AU  - Vučinić, Violeta
AU  - Skodrić-Trifunović, Vesna
AU  - Ignjatović, Svetlana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1534
AB  - Purpose of review Calcium metabolism impairments have long been recognized as a complication of sarcoidosis. For more than six decades physicians and investigators have been trying to elucidate this severe problem; nevertheless it seems puzzlingly new for both readers and researchers. Recent findings This review highlights the problems of calcium metabolism in sarcoidosis in relation to vitamin D synthesis, which is definitely altered by granulomatous inflammation. Increasing evidence suggests that vitamin D is an immunomodulating hormone that inhibits both antigen presentation by cells of the innate immune system, and the cytokine release and proliferation of Th1 cells. As calcium homeostasis is primary controlled by levels of vitamin D, parathyroid hormone (PTH) and calcitonin, this literature review emphasizes the role of general immunomodulating properties of vitamin D and the correlation with calcium metabolism impairments, with the special accent on already known interactions with sarcoidosis. Summary Granuloma formation has been related to a failure of the innate immune system. One of the possible explanations is a vitamin D deficiency. The evidence-based findings on calcium metabolism impairments and the interactions with vitamin D might help both clinicians and researchers in developing new strategies.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Current Opinion in Pulmonary Medicine
T1  - How to diagnose and manage difficult problems of calcium metabolism in sarcoidosis: an evidence-based review
VL  - 17
IS  - 5
SP  - 297
EP  - 302
DO  - 10.1097/MCP.0b013e328348b3cb
ER  - 

@article{
author = "Vučinić, Violeta and Skodrić-Trifunović, Vesna and Ignjatović, Svetlana",
year = "2011",
abstract = "Purpose of review Calcium metabolism impairments have long been recognized as a complication of sarcoidosis. For more than six decades physicians and investigators have been trying to elucidate this severe problem; nevertheless it seems puzzlingly new for both readers and researchers. Recent findings This review highlights the problems of calcium metabolism in sarcoidosis in relation to vitamin D synthesis, which is definitely altered by granulomatous inflammation. Increasing evidence suggests that vitamin D is an immunomodulating hormone that inhibits both antigen presentation by cells of the innate immune system, and the cytokine release and proliferation of Th1 cells. As calcium homeostasis is primary controlled by levels of vitamin D, parathyroid hormone (PTH) and calcitonin, this literature review emphasizes the role of general immunomodulating properties of vitamin D and the correlation with calcium metabolism impairments, with the special accent on already known interactions with sarcoidosis. Summary Granuloma formation has been related to a failure of the innate immune system. One of the possible explanations is a vitamin D deficiency. The evidence-based findings on calcium metabolism impairments and the interactions with vitamin D might help both clinicians and researchers in developing new strategies.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Current Opinion in Pulmonary Medicine",
title = "How to diagnose and manage difficult problems of calcium metabolism in sarcoidosis: an evidence-based review",
volume = "17",
number = "5",
pages = "297-302",
doi = "10.1097/MCP.0b013e328348b3cb"
}

Vučinić, V., Skodrić-Trifunović, V.,& Ignjatović, S.. (2011). How to diagnose and manage difficult problems of calcium metabolism in sarcoidosis: an evidence-based review. in Current Opinion in Pulmonary Medicine
Lippincott Williams & Wilkins, Philadelphia., 17(5), 297-302.
https://doi.org/10.1097/MCP.0b013e328348b3cb

Vučinić V, Skodrić-Trifunović V, Ignjatović S. How to diagnose and manage difficult problems of calcium metabolism in sarcoidosis: an evidence-based review. in Current Opinion in Pulmonary Medicine. 2011;17(5):297-302.
doi:10.1097/MCP.0b013e328348b3cb .

Vučinić, Violeta, Skodrić-Trifunović, Vesna, Ignjatović, Svetlana, "How to diagnose and manage difficult problems of calcium metabolism in sarcoidosis: an evidence-based review" in Current Opinion in Pulmonary Medicine, 17, no. 5 (2011):297-302,
https://doi.org/10.1097/MCP.0b013e328348b3cb . .