TY  - JOUR
AU  - Miletić Vukajlović, Jadranka
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Ilić, Tihomir V.
AU  - Stefanović, Aleksandra
AU  - Petković, Marijana
AU  - Schiller, Jürgen
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3522
AB  - Rationale: Changes in lipid composition might be associated with the onset and progression of various neurodegenerative diseases. Herein, we investigated the changes in the plasma phosphatidylcholine (PC)/lysophosphatidylcholine (LPC) ratios in patients with Parkinson's disease (PD) in comparison with healthy subjects and their correlation with clinico-pathological features. Methods: The study included 10 controls and 25 patients with PD. All patients were assigned to groups based on clinico-pathological characteristics (gender, age at examination, duration of disease and Hoehn and Yahr (H&Y) stage). The analysis of the PC/LPC intensity ratios in plasma lipid extracts was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: PD patients exhibited an increased PC/LPC intensity ratio in comparison with the control group of healthy subjects. Furthermore, the investigated ratio was shown to be correlated with clinico-pathological parameters, in particular with H&Y stage and disease duration. The PC/LPC intensity ratio in plasma samples of PD patients was found to be elevated in all examined H&Y stages and throughout the disease duration. Conclusions: To our knowledge, this is the first study examining the PC/LPC ratios in plasma of patients with PD and illustrating their correlation with clinico-pathological features. Although the presented results may be considered as preliminary due to the limited number of participants, the observed alterations of PC/LPC ratios in plasma might be a first step in the characterization of plasma lipid changes in PD patients and an indicator of lipid reconfiguration.
PB  - John Wiley and Sons Ltd
T2  - Rapid Communications in Mass Spectrometry
T1  - Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
VL  - 34
IS  - 4
DO  - 10.1002/rcm.8595
ER  - 

@article{
author = "Miletić Vukajlović, Jadranka and Drakulić, Dunja and Pejić, Snežana and Ilić, Tihomir V. and Stefanović, Aleksandra and Petković, Marijana and Schiller, Jürgen",
year = "2020",
abstract = "Rationale: Changes in lipid composition might be associated with the onset and progression of various neurodegenerative diseases. Herein, we investigated the changes in the plasma phosphatidylcholine (PC)/lysophosphatidylcholine (LPC) ratios in patients with Parkinson's disease (PD) in comparison with healthy subjects and their correlation with clinico-pathological features. Methods: The study included 10 controls and 25 patients with PD. All patients were assigned to groups based on clinico-pathological characteristics (gender, age at examination, duration of disease and Hoehn and Yahr (H&Y) stage). The analysis of the PC/LPC intensity ratios in plasma lipid extracts was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: PD patients exhibited an increased PC/LPC intensity ratio in comparison with the control group of healthy subjects. Furthermore, the investigated ratio was shown to be correlated with clinico-pathological parameters, in particular with H&Y stage and disease duration. The PC/LPC intensity ratio in plasma samples of PD patients was found to be elevated in all examined H&Y stages and throughout the disease duration. Conclusions: To our knowledge, this is the first study examining the PC/LPC ratios in plasma of patients with PD and illustrating their correlation with clinico-pathological features. Although the presented results may be considered as preliminary due to the limited number of participants, the observed alterations of PC/LPC ratios in plasma might be a first step in the characterization of plasma lipid changes in PD patients and an indicator of lipid reconfiguration.",
publisher = "John Wiley and Sons Ltd",
journal = "Rapid Communications in Mass Spectrometry",
title = "Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease",
volume = "34",
number = "4",
doi = "10.1002/rcm.8595"
}

Miletić Vukajlović, J., Drakulić, D., Pejić, S., Ilić, T. V., Stefanović, A., Petković, M.,& Schiller, J.. (2020). Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease. in Rapid Communications in Mass Spectrometry
John Wiley and Sons Ltd., 34(4).
https://doi.org/10.1002/rcm.8595

Miletić Vukajlović J, Drakulić D, Pejić S, Ilić TV, Stefanović A, Petković M, Schiller J. Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease. in Rapid Communications in Mass Spectrometry. 2020;34(4).
doi:10.1002/rcm.8595 .

Miletić Vukajlović, Jadranka, Drakulić, Dunja, Pejić, Snežana, Ilić, Tihomir V., Stefanović, Aleksandra, Petković, Marijana, Schiller, Jürgen, "Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease" in Rapid Communications in Mass Spectrometry, 34, no. 4 (2020),
https://doi.org/10.1002/rcm.8595 . .

TY  - JOUR
AU  - Miletić, Jadranka
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Petković, Marijana
AU  - Ilić, Tihomir V.
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Prostran, Milica
AU  - Stojanov, Marina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3163
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3420
AB  - Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
VL  - 128
IS  - 7
SP  - 600
EP  - 607
DO  - 10.1080/00207454.2017.1403916
ER  - 

@article{
author = "Miletić, Jadranka and Drakulić, Dunja and Pejić, Snežana and Petković, Marijana and Ilić, Tihomir V. and Miljković, Milica and Stefanović, Aleksandra and Prostran, Milica and Stojanov, Marina",
year = "2018",
abstract = "Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease",
volume = "128",
number = "7",
pages = "600-607",
doi = "10.1080/00207454.2017.1403916"
}

Miletić, J., Drakulić, D., Pejić, S., Petković, M., Ilić, T. V., Miljković, M., Stefanović, A., Prostran, M.,& Stojanov, M.. (2018). Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 128(7), 600-607.
https://doi.org/10.1080/00207454.2017.1403916

Miletić J, Drakulić D, Pejić S, Petković M, Ilić TV, Miljković M, Stefanović A, Prostran M, Stojanov M. Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience. 2018;128(7):600-607.
doi:10.1080/00207454.2017.1403916 .

Miletić, Jadranka, Drakulić, Dunja, Pejić, Snežana, Petković, Marijana, Ilić, Tihomir V., Miljković, Milica, Stefanović, Aleksandra, Prostran, Milica, Stojanov, Marina, "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease" in International Journal of Neuroscience, 128, no. 7 (2018):600-607,
https://doi.org/10.1080/00207454.2017.1403916 . .

TY  - JOUR
AU  - Miletić, Jadranka
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Petković, Marijana
AU  - Ilić, Tihomir V.
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Prostran, Milica
AU  - Stojanov, Marina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3163
AB  - Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
VL  - 128
IS  - 7
SP  - 600
EP  - 607
DO  - 10.1080/00207454.2017.1403916
ER  - 

@article{
author = "Miletić, Jadranka and Drakulić, Dunja and Pejić, Snežana and Petković, Marijana and Ilić, Tihomir V. and Miljković, Milica and Stefanović, Aleksandra and Prostran, Milica and Stojanov, Marina",
year = "2018",
abstract = "Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease",
volume = "128",
number = "7",
pages = "600-607",
doi = "10.1080/00207454.2017.1403916"
}

Miletić, J., Drakulić, D., Pejić, S., Petković, M., Ilić, T. V., Miljković, M., Stefanović, A., Prostran, M.,& Stojanov, M.. (2018). Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 128(7), 600-607.
https://doi.org/10.1080/00207454.2017.1403916

Miletić J, Drakulić D, Pejić S, Petković M, Ilić TV, Miljković M, Stefanović A, Prostran M, Stojanov M. Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience. 2018;128(7):600-607.
doi:10.1080/00207454.2017.1403916 .

Miletić, Jadranka, Drakulić, Dunja, Pejić, Snežana, Petković, Marijana, Ilić, Tihomir V., Miljković, Milica, Stefanović, Aleksandra, Prostran, Milica, Stojanov, Marina, "Prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease" in International Journal of Neuroscience, 128, no. 7 (2018):600-607,
https://doi.org/10.1080/00207454.2017.1403916 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Micić, Mileva
AU  - Filipović, Jelena
AU  - Drakulić, Dunja
AU  - Stanojlović, Miloš
AU  - Čalija, Bojan
AU  - Valenta Sobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3015
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Micić, Mileva and Filipović, Jelena and Drakulić, Dunja and Stanojlović, Miloš and Čalija, Bojan and Valenta Sobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Micić, M., Filipović, J., Drakulić, D., Stanojlović, M., Čalija, B., Valenta Sobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Micić M, Filipović J, Drakulić D, Stanojlović M, Čalija B, Valenta Sobot A, Demajo M, Nilsson R. Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Micić, Mileva, Filipović, Jelena, Drakulić, Dunja, Stanojlović, Miloš, Čalija, Bojan, Valenta Sobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach" in Acta veterinaria, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .