TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić-Matutinović, Marija
AU  - Pejanović, Svetlana
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3617
AB  - Background: Problem  of  the  variability  between  the different  methods  using  for  bone  alkaline  phosphatase(bALP)  determination  greately  influences  the  clinical significance of bALP as direct marker of bone metabolism.The  aim  of  this  study  was  to  compare  immunoassay  with electrophoresis technique for bALP determination.  Methods: We  measured  bALP  in  71  patients  on  hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter).Results: The   analyzed   methods   showed   significant correlation  (Spearman’s  rho:  0.776,  P  <  0.01),  but  we found statistically significant (P < 0.01) positive bias (27%)for  the  results  measured  by  immunoassay.  In  support  of this,  using  electrophoresis  technique  we  have  detected presence  of  the  intestinal  isoenzymes  of  alkaline  phosphatase in 55% of patients with median value of 30% of the total  alkaline  phoshatase  and  presence  of  liver-2  alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant  concordance  between  two  analysed  methods.Cusum  test  showed  no  significant  deviation  from  linearity(P=0.850).Conclusions:Despite  good  agreement  between  immunoassay  methods  and  electrophoresis  technique  for  bALP determination,  interchangeability  between  these  two methods  is  questionable.  Although  immunoassays  are increasingly  used,  as  fully  automated  methods,  in  a  large number  of  laboratories  and  become  routine  methods  for bALP determination, it should be beared in mind, besidesvarious  interferences,  also  the  heterogeneity  of  the  bALP itself, especially in patients on hemodialysis.
AB  - Uvod: Problem varijabilnosti između različitih metoda pomoću kojih se određuje koštani izoezim alkalne fosfataze (bALP) u velikoj meri utiče na klinički značaj bALP kao direktnog markera metabolizma kostiju. Cilj ove studije bio je poređenje imunohemijskog testa sa elektroforetskom tehnikom za određivanje bALP. Metode: bALP je određen kod 71 pacijenta na hemodijalizi, agar gel elektroforetskom tehnikom (ISO-PAL, SEBIA) i imunohemijskom metodom (OSTASE, Beckman Coulter). Rezultati: Analizirane metode pokazale su značajno slaganje (Spearmanov rho: 0,776, P < 0,01), ali je uočen statistički značajan (P < 0,01) pozitivan bias (27%) za rezultate izmerene imunohemijskom metodom. U prilog ovome, primenom elektroforetske tehnike, otkrili smo prisustvo intestinalnih izoenzima alkalne fosfataze u 55% bolesnika, sa srednjom vrednosti od 30% ukupne alkalne fostataze, kao i prisustvo jetrenih izoformi alkalne fosfataze kod 42% bolesnika sa srednjom vrednosti od 16,6%. Kendall-ov W od 0,787 (P < 0,0001) ukazivao je na značajno slaganje između dve analizirane metode. Cusumov test nije pokazao značajno odstupanje od linearnosti (P = 0,850). Zaključak: Uprkos tome što postoji dobro slaganje između imunohemijske metode i elektroforetske tehnike za određivanje bALP-a, međusobna zamenjivost ove dve metode je pod znakom pitanja. Iako se imunohemijske metode sve više koriste kao potpuno automatizovane, u velikom broju laboratorija i postaju rutinske metode za određivanje bALP-a, treba imati na umu, osim različitih interferencija, i heterogenost samog bALP, posebno kod bolesnika na hemodijalizi.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients
T1  - Poređenje imunohemijske metode i elektroforetske tehnike za određivanje koštanog izoenzima alkalne fosfataze kod pacijenata na hemodijalizi
VL  - 39
IS  - 2
SP  - 178
EP  - 183
DO  - 10.2478/jomb-2019-0048
ER  - 

@article{
author = "Milinković, Neda and Sarić-Matutinović, Marija and Pejanović, Svetlana and Ignjatović, Svetlana",
year = "2020",
abstract = "Background: Problem  of  the  variability  between  the different  methods  using  for  bone  alkaline  phosphatase(bALP)  determination  greately  influences  the  clinical significance of bALP as direct marker of bone metabolism.The  aim  of  this  study  was  to  compare  immunoassay  with electrophoresis technique for bALP determination.  Methods: We  measured  bALP  in  71  patients  on  hemodialysis with agar gel electrophoresis (ISO-PAL, SEBIA) and immunoassay (OSTASE, Beckman Coulter).Results: The   analyzed   methods   showed   significant correlation  (Spearman’s  rho:  0.776,  P  <  0.01),  but  we found statistically significant (P < 0.01) positive bias (27%)for  the  results  measured  by  immunoassay.  In  support  of this,  using  electrophoresis  technique  we  have  detected presence  of  the  intestinal  isoenzymes  of  alkaline  phosphatase in 55% of patients with median value of 30% of the total  alkaline  phoshatase  and  presence  of  liver-2  alkaline phosphatase isoform in 42% of patients with median value of 16.6%. The Kendall’s W of 0.787 (P<0.0001) revealed significant  concordance  between  two  analysed  methods.Cusum  test  showed  no  significant  deviation  from  linearity(P=0.850).Conclusions:Despite  good  agreement  between  immunoassay  methods  and  electrophoresis  technique  for  bALP determination,  interchangeability  between  these  two methods  is  questionable.  Although  immunoassays  are increasingly  used,  as  fully  automated  methods,  in  a  large number  of  laboratories  and  become  routine  methods  for bALP determination, it should be beared in mind, besidesvarious  interferences,  also  the  heterogeneity  of  the  bALP itself, especially in patients on hemodialysis., Uvod: Problem varijabilnosti između različitih metoda pomoću kojih se određuje koštani izoezim alkalne fosfataze (bALP) u velikoj meri utiče na klinički značaj bALP kao direktnog markera metabolizma kostiju. Cilj ove studije bio je poređenje imunohemijskog testa sa elektroforetskom tehnikom za određivanje bALP. Metode: bALP je određen kod 71 pacijenta na hemodijalizi, agar gel elektroforetskom tehnikom (ISO-PAL, SEBIA) i imunohemijskom metodom (OSTASE, Beckman Coulter). Rezultati: Analizirane metode pokazale su značajno slaganje (Spearmanov rho: 0,776, P < 0,01), ali je uočen statistički značajan (P < 0,01) pozitivan bias (27%) za rezultate izmerene imunohemijskom metodom. U prilog ovome, primenom elektroforetske tehnike, otkrili smo prisustvo intestinalnih izoenzima alkalne fosfataze u 55% bolesnika, sa srednjom vrednosti od 30% ukupne alkalne fostataze, kao i prisustvo jetrenih izoformi alkalne fosfataze kod 42% bolesnika sa srednjom vrednosti od 16,6%. Kendall-ov W od 0,787 (P < 0,0001) ukazivao je na značajno slaganje između dve analizirane metode. Cusumov test nije pokazao značajno odstupanje od linearnosti (P = 0,850). Zaključak: Uprkos tome što postoji dobro slaganje između imunohemijske metode i elektroforetske tehnike za određivanje bALP-a, međusobna zamenjivost ove dve metode je pod znakom pitanja. Iako se imunohemijske metode sve više koriste kao potpuno automatizovane, u velikom broju laboratorija i postaju rutinske metode za određivanje bALP-a, treba imati na umu, osim različitih interferencija, i heterogenost samog bALP, posebno kod bolesnika na hemodijalizi.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients, Poređenje imunohemijske metode i elektroforetske tehnike za određivanje koštanog izoenzima alkalne fosfataze kod pacijenata na hemodijalizi",
volume = "39",
number = "2",
pages = "178-183",
doi = "10.2478/jomb-2019-0048"
}

Milinković, N., Sarić-Matutinović, M., Pejanović, S.,& Ignjatović, S.. (2020). Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(2), 178-183.
https://doi.org/10.2478/jomb-2019-0048

Milinković N, Sarić-Matutinović M, Pejanović S, Ignjatović S. Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients. in Journal of Medical Biochemistry. 2020;39(2):178-183.
doi:10.2478/jomb-2019-0048 .

Milinković, Neda, Sarić-Matutinović, Marija, Pejanović, Svetlana, Ignjatović, Svetlana, "Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients" in Journal of Medical Biochemistry, 39, no. 2 (2020):178-183,
https://doi.org/10.2478/jomb-2019-0048 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Majkić-Singh, Nada
AU  - Ignjatović, Svetlana
AU  - Lezaić, Višnja D.
AU  - Pejanović, Svetlana D.
AU  - Jovanović, Dijana B.
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1638
AB  - Background: Bone alkaline phosphatase (BALP) is a direct and independent indicator of impaired bone turnover. We intended to find out whether there are any significant changes in BALP and iPTH levels, in comparison to total Ca, total Mg, inorganic P, total alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) in predialysis and dialysis patients. Methods: Out of 266 patients investigated, 114 were on continuous ambulatory peritoneal dialysis, 112 were on maintenance haemodialysis, while 40 predialysis patients had end stage renal disease. The parameters were analysed according to the manufacturers' instructions. Results: Correlations were established for the bone marker concentrations analysed among the studied groups. The largest ranges were determined for BALP and iPTH. Predialysis and dialysis patients showed very low levels of BALP. Dialysis patients had lower levels of iPTH (p lt 0.001), while in predialysis patients the levels were significantly higher (p lt 0.05) than recommended for low bone turnover, according to K/DOQI. Conclusions: The observations made in this study identify BALP as a good indicator of decreased bone turnover in predialysis and dialysis patients. However, in order to reveal a difference between bone activity and the level of parathyroid activity and its effect on bone turnover, it is always necessary to observe both BALP and iPTH levels. (Clin. Lab. 2012;58:747-753. DOI: 10.7754/Clin.Lab.2011.110816)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients
VL  - 58
IS  - 7-8
SP  - 747
EP  - 753
DO  - 10.7754/Clin.Lab.2011.110816
ER  - 

@article{
author = "Milinković, Neda and Majkić-Singh, Nada and Ignjatović, Svetlana and Lezaić, Višnja D. and Pejanović, Svetlana D. and Jovanović, Dijana B.",
year = "2012",
abstract = "Background: Bone alkaline phosphatase (BALP) is a direct and independent indicator of impaired bone turnover. We intended to find out whether there are any significant changes in BALP and iPTH levels, in comparison to total Ca, total Mg, inorganic P, total alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) in predialysis and dialysis patients. Methods: Out of 266 patients investigated, 114 were on continuous ambulatory peritoneal dialysis, 112 were on maintenance haemodialysis, while 40 predialysis patients had end stage renal disease. The parameters were analysed according to the manufacturers' instructions. Results: Correlations were established for the bone marker concentrations analysed among the studied groups. The largest ranges were determined for BALP and iPTH. Predialysis and dialysis patients showed very low levels of BALP. Dialysis patients had lower levels of iPTH (p lt 0.001), while in predialysis patients the levels were significantly higher (p lt 0.05) than recommended for low bone turnover, according to K/DOQI. Conclusions: The observations made in this study identify BALP as a good indicator of decreased bone turnover in predialysis and dialysis patients. However, in order to reveal a difference between bone activity and the level of parathyroid activity and its effect on bone turnover, it is always necessary to observe both BALP and iPTH levels. (Clin. Lab. 2012;58:747-753. DOI: 10.7754/Clin.Lab.2011.110816)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients",
volume = "58",
number = "7-8",
pages = "747-753",
doi = "10.7754/Clin.Lab.2011.110816"
}

Milinković, N., Majkić-Singh, N., Ignjatović, S., Lezaić, V. D., Pejanović, S. D.,& Jovanović, D. B.. (2012). Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(7-8), 747-753.
https://doi.org/10.7754/Clin.Lab.2011.110816

Milinković N, Majkić-Singh N, Ignjatović S, Lezaić VD, Pejanović SD, Jovanović DB. Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients. in Clinical Laboratory. 2012;58(7-8):747-753.
doi:10.7754/Clin.Lab.2011.110816 .

Milinković, Neda, Majkić-Singh, Nada, Ignjatović, Svetlana, Lezaić, Višnja D., Pejanović, Svetlana D., Jovanović, Dijana B., "Correlation of Bone Alkaline Phosphatase and iPTH with Some Basic Biochemical Markers in Predialysis and Dialysis Patients" in Clinical Laboratory, 58, no. 7-8 (2012):747-753,
https://doi.org/10.7754/Clin.Lab.2011.110816 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Majkić-Singh, Nada
AU  - Mirković, Duško
AU  - Beletić, Anđelo
AU  - Pejanović, Svetlana D.
AU  - Vujanić, Svetlana T.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1189
AB  - Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients
VL  - 55
IS  - 9-10
SP  - 333
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1189
ER  - 

@article{
author = "Milinković, Neda and Majkić-Singh, Nada and Mirković, Duško and Beletić, Anđelo and Pejanović, Svetlana D. and Vujanić, Svetlana T.",
year = "2009",
abstract = "Deficient serum 25-hydroxyvitamin D [25(OH)D] may contribute to the impaired bone turnover of end stage renal disease patients. In 112 hemodialysed patients we analysed the relation between 25(OH)D and bone alkaline phosphatase (BALP), beta-CrossLaps (beta-CTx) and iPTH. We analysed parameters according to the manufacturers' instructions. We found potentially significant vitamin D deficiency: 71% of patients had 25(OH)D levels below 50 nmol/L. In patients with iPTH below 150 pg/mL (n = 57), we observed significantly low 25(OH) (p lt 0.01). In addition, patients with iPTH above 300 pg/mL had higher BALP levels (p lt 0.05). There were negative correlations between serum 25(OH)D and both BALP and iPTH (r = -0.225, p lt 0.05 and r = -0.331, p lt 0.05). Beta-CTx levels were significantly higher in patients who did not receive vitamin D supplementation (p lt 0.01). In addition, reduced BALP and iPTH levels indicate decreased bone turnover. Recorded data could signify that vitamin D deficiency may contribute to the impaired bone metabolism of hemodialysis patients. (Clin. Lab. 2009;55:333-339)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients",
volume = "55",
number = "9-10",
pages = "333-339",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1189"
}

Milinković, N., Majkić-Singh, N., Mirković, D., Beletić, A., Pejanović, S. D.,& Vujanić, S. T.. (2009). Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 55(9-10), 333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189

Milinković N, Majkić-Singh N, Mirković D, Beletić A, Pejanović SD, Vujanić ST. Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients. in Clinical Laboratory. 2009;55(9-10):333-339.
https://hdl.handle.net/21.15107/rcub_farfar_1189 .

Milinković, Neda, Majkić-Singh, Nada, Mirković, Duško, Beletić, Anđelo, Pejanović, Svetlana D., Vujanić, Svetlana T., "Relation Between 25(OH)-Vitamin D Deficiency and Markers of Bone Formation and Resorption in Haemodialysis Patients" in Clinical Laboratory, 55, no. 9-10 (2009):333-339,
https://hdl.handle.net/21.15107/rcub_farfar_1189 .