TY  - JOUR
AU  - Perović-Blagojević, Iva
AU  - Vekić, Jelena
AU  - Macut, Đuro
AU  - Ignjatović, Svetlana
AU  - Miljković-Trailović, Milica
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Božić-Antić, Ivana
AU  - Bjekić-Macut, Jelica
AU  - Kastratović-Kotlica, Biljana
AU  - Andrić, Zoran
AU  - Ilić, Dušan
AU  - Kotur-Stevuljević, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3976
AB  - Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.
PB  - Cambridge University Press
T2  - British Journal of Nutrition
T1  - Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia
VL  - 128
IS  - 4
SP  - 604
EP  - 612
DO  - 10.1017/S0007114521003585
ER  - 

@article{
author = "Perović-Blagojević, Iva and Vekić, Jelena and Macut, Đuro and Ignjatović, Svetlana and Miljković-Trailović, Milica and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Božić-Antić, Ivana and Bjekić-Macut, Jelica and Kastratović-Kotlica, Biljana and Andrić, Zoran and Ilić, Dušan and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "Objective: Polycystic ovary syndrome (PCOS) is associated with altered lipid profile and increased small, dense LDL particles (sdLDL). Considering that paraoxonase 1 (PON1) is an anti-oxidative enzyme located on high-density lipoprotein (HDL) particles, the aim of this study was to investigate the connection between oxidative stress (OS) and PON1 activity with lipoprotein subclasses in PCOS depending on obesity. Methods: In 115 PCOS patients lipoprotein subclasses distributions were determined by gradient gel electrophoresis. OS status was assessed by total oxidative status (TOS), advanced oxidation protein products (AOPP), malondialdehyde (MDA), prooxidant-Antioxidant balance (PAB), total antioxidant status (TAS) and superoxide dismutase (SOD) and PON1 activity. Results: Overweight/obese PCOS patients (n=55) had increased OS compared to normal weight patients (n=60). In addition, overweight/obese group had lower HDL size and higher proportion of HDL 3a subclasses (P<0.05). PAB was in negative correlation with HDL 2a (P<0.001), whereas MDA and SOD correlated positively with HDL 3 subclasses (P<0.05). Serum PON1 activity was positively associated with proportions of PON1 activity on HDL 2b (P<0.05) and 2a (P<0.01), but negatively with the proportion on HDL 3 particles (P<0.01). LDL B phenotype patients had increased TAS, SOD and PON1 activity on HDL 2b, but decreased PON1 activity on HDL 3 subclasses. Conclusion: OS is associated with altered lipoprotein subclasses distribution in PCOS patients. Obesity in PCOS affects the profile of HDL subclasses, reflected through the reduced proportion of PON1 activity on HDL 3 subclasses in the presence of sdLDL particles.",
publisher = "Cambridge University Press",
journal = "British Journal of Nutrition",
title = "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia",
volume = "128",
number = "4",
pages = "604-612",
doi = "10.1017/S0007114521003585"
}

Perović-Blagojević, I., Vekić, J., Macut, Đ., Ignjatović, S., Miljković-Trailović, M., Zeljković, A., Spasojević-Kalimanovska, V., Božić-Antić, I., Bjekić-Macut, J., Kastratović-Kotlica, B., Andrić, Z., Ilić, D.,& Kotur-Stevuljević, J.. (2022). Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition
Cambridge University Press., 128(4), 604-612.
https://doi.org/10.1017/S0007114521003585

Perović-Blagojević I, Vekić J, Macut Đ, Ignjatović S, Miljković-Trailović M, Zeljković A, Spasojević-Kalimanovska V, Božić-Antić I, Bjekić-Macut J, Kastratović-Kotlica B, Andrić Z, Ilić D, Kotur-Stevuljević J. Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia. in British Journal of Nutrition. 2022;128(4):604-612.
doi:10.1017/S0007114521003585 .

Perović-Blagojević, Iva, Vekić, Jelena, Macut, Đuro, Ignjatović, Svetlana, Miljković-Trailović, Milica, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Božić-Antić, Ivana, Bjekić-Macut, Jelica, Kastratović-Kotlica, Biljana, Andrić, Zoran, Ilić, Dušan, Kotur-Stevuljević, Jelena, "Overweight and Obesity in Polycystic Ovary Syndrome: Association with Inflammation, Oxidative Stress and Dyslipidemia" in British Journal of Nutrition, 128, no. 4 (2022):604-612,
https://doi.org/10.1017/S0007114521003585 . .