TY  - CONF
AU  - Marić, Đurđica
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Mandić-Rajčević, Stefan
AU  - Žarković, Miloš
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Buha-Đorđević, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5426
AB  - Uticaj toksičnog metala nikla (Ni) na funkciju štitaste žlezde još uvek nije dovoljno istražen.
Stoga je cilj ove studije bio da se ispita potencijal Ni da poremeti funkciju štitaste žlezde ispitivanjem
odnosa između koncentracije Ni u krvi i nivoa hormona u serumu (TSH, T4, T3, fT4, fT3), kao i parametara
tiroidne homeostaze (SPINA-GT i SPINA-GD) korišćenjem korelacione analize i Benchmark (BMD) koncepta. ...
AB  - Nickel (Ni) is one of the well-known toxic metals whose effect on thyroid function has not been suffi-
ciently investigated yet. The aim of this study was to analyse the potential of Ni to disrupt thyroid func-
tion by exploring the relationship between blood Ni concentration and serum hormone levels (TSH,
T4, T3, fT4, fT3), as well as the parameters of thyroid homeostasis (SPINA-GT and SPINA-GD) by using
correlation analysis and Benchmark (BMD) concept ...
PB  - Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
T1  - Veza između izloženosti niklu i poremećaja funkcije štitaste žlezde - rezultati studije humanog biomonitoringa
T1  - The relationship between nickel exposure and thyroid dysfunction - results of a human biomonitoring study
SP  - 63
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5426
ER  - 

@conference{
author = "Marić, Đurđica and Baralić, Katarina and Vukelić, Dragana and Mandić-Rajčević, Stefan and Žarković, Miloš and Antonijević, Biljana and Đukić-Ćosić, Danijela and Bulat, Zorica and Buha-Đorđević, Aleksandra",
year = "2023",
abstract = "Uticaj toksičnog metala nikla (Ni) na funkciju štitaste žlezde još uvek nije dovoljno istražen.
Stoga je cilj ove studije bio da se ispita potencijal Ni da poremeti funkciju štitaste žlezde ispitivanjem
odnosa između koncentracije Ni u krvi i nivoa hormona u serumu (TSH, T4, T3, fT4, fT3), kao i parametara
tiroidne homeostaze (SPINA-GT i SPINA-GD) korišćenjem korelacione analize i Benchmark (BMD) koncepta. ..., Nickel (Ni) is one of the well-known toxic metals whose effect on thyroid function has not been suffi-
ciently investigated yet. The aim of this study was to analyse the potential of Ni to disrupt thyroid func-
tion by exploring the relationship between blood Ni concentration and serum hormone levels (TSH,
T4, T3, fT4, fT3), as well as the parameters of thyroid homeostasis (SPINA-GT and SPINA-GD) by using
correlation analysis and Benchmark (BMD) concept ...",
publisher = "Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book",
title = "Veza između izloženosti niklu i poremećaja funkcije štitaste žlezde - rezultati studije humanog biomonitoringa, The relationship between nickel exposure and thyroid dysfunction - results of a human biomonitoring study",
pages = "63-64",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5426"
}

Marić, Đ., Baralić, K., Vukelić, D., Mandić-Rajčević, S., Žarković, M., Antonijević, B., Đukić-Ćosić, D., Bulat, Z.,& Buha-Đorđević, A.. (2023). Veza između izloženosti niklu i poremećaja funkcije štitaste žlezde - rezultati studije humanog biomonitoringa. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
Udruženje toksikologa Srbije., 63-64.
https://hdl.handle.net/21.15107/rcub_farfar_5426

Marić Đ, Baralić K, Vukelić D, Mandić-Rajčević S, Žarković M, Antonijević B, Đukić-Ćosić D, Bulat Z, Buha-Đorđević A. Veza između izloženosti niklu i poremećaja funkcije štitaste žlezde - rezultati studije humanog biomonitoringa. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book. 2023;:63-64.
https://hdl.handle.net/21.15107/rcub_farfar_5426 .

Marić, Đurđica, Baralić, Katarina, Vukelić, Dragana, Mandić-Rajčević, Stefan, Žarković, Miloš, Antonijević, Biljana, Đukić-Ćosić, Danijela, Bulat, Zorica, Buha-Đorđević, Aleksandra, "Veza između izloženosti niklu i poremećaja funkcije štitaste žlezde - rezultati studije humanog biomonitoringa" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book (2023):63-64,
https://hdl.handle.net/21.15107/rcub_farfar_5426 .

TY  - JOUR
AU  - Marić, Đurđica
AU  - Baralić, Katarina
AU  - Javorac, Dragana
AU  - Mandić-Rajčević, Stefan
AU  - Žarković, Miloš
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Buha-Đorđević, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5144
AB  - Introduction: Nickel (Ni) is one of the well-known toxic metals found in the environment. However, its influence on thyroid function is not explored enough. Hence, the aim of this study was to analyse the potential of Ni to disrupt thyroid function by exploring the relationship between blood Ni concentration and serum hormone levels (TSH, T4, T3, fT4 and fT3), as well as the parameters of thyroid homeostasis (SPINA-GT and SPINA-GD) by using correlation analysis and Benchmark (BMD) concept. Methods: Ni concentration was measured by ICP-MS method, while CLIA was used for serum hormone determination. SPINA Thyr software was used to calculate SPINA-GT and SPINA-GD parameters. BMD analysis was performed by PROAST software (70.1). The limitations of this study are the small sample size and the uneven distribution of healthy and unhealthy subjects, limited confounding factors, as well as the age of the subjects that could have influenced the obtained results. Results and discussion: The highest median value for blood Ni concentration was observed for the male population and amounted 8,278 µg/L. Accordingly, the statistically significant correlation was observed only in the male population, for Ni-fT4 and Ni-SPINA-GT pairs. The existence of a dose-response relationship was established between Ni and all the measured parameters of thyroid functions in entire population and in both sexes. However, the narrowest BMD intervals were obtained only in men, for Ni - SPINA-GT pair (1.36-60.9 µg/L) and Ni - fT3 pair (0.397-66.8 µg/L), indicating that even 78.68 and 83.25% of men in our study might be in 10% higher risk of Ni-induced SPINA-GT and fT3 alterations, respectively. Due to the relationship established between Ni and the SPINA-GT parameter, it can be concluded that Ni has an influence on the secretory function of the thyroid gland in men. Although the further research is required, these findings suggest possible role of Ni in thyroid function disturbances. Copyright
PB  - Frontiers Media SA
T2  - Frontiers in Endocrinology
T1  - Nickel as a potential disruptor of thyroid function: benchmark modelling of human data
VL  - 14
DO  - 10.3389/fendo.2023.1145153
ER  - 

@article{
author = "Marić, Đurđica and Baralić, Katarina and Javorac, Dragana and Mandić-Rajčević, Stefan and Žarković, Miloš and Antonijević, Biljana and Đukić-Ćosić, Danijela and Bulat, Zorica and Buha-Đorđević, Aleksandra",
year = "2023",
abstract = "Introduction: Nickel (Ni) is one of the well-known toxic metals found in the environment. However, its influence on thyroid function is not explored enough. Hence, the aim of this study was to analyse the potential of Ni to disrupt thyroid function by exploring the relationship between blood Ni concentration and serum hormone levels (TSH, T4, T3, fT4 and fT3), as well as the parameters of thyroid homeostasis (SPINA-GT and SPINA-GD) by using correlation analysis and Benchmark (BMD) concept. Methods: Ni concentration was measured by ICP-MS method, while CLIA was used for serum hormone determination. SPINA Thyr software was used to calculate SPINA-GT and SPINA-GD parameters. BMD analysis was performed by PROAST software (70.1). The limitations of this study are the small sample size and the uneven distribution of healthy and unhealthy subjects, limited confounding factors, as well as the age of the subjects that could have influenced the obtained results. Results and discussion: The highest median value for blood Ni concentration was observed for the male population and amounted 8,278 µg/L. Accordingly, the statistically significant correlation was observed only in the male population, for Ni-fT4 and Ni-SPINA-GT pairs. The existence of a dose-response relationship was established between Ni and all the measured parameters of thyroid functions in entire population and in both sexes. However, the narrowest BMD intervals were obtained only in men, for Ni - SPINA-GT pair (1.36-60.9 µg/L) and Ni - fT3 pair (0.397-66.8 µg/L), indicating that even 78.68 and 83.25% of men in our study might be in 10% higher risk of Ni-induced SPINA-GT and fT3 alterations, respectively. Due to the relationship established between Ni and the SPINA-GT parameter, it can be concluded that Ni has an influence on the secretory function of the thyroid gland in men. Although the further research is required, these findings suggest possible role of Ni in thyroid function disturbances. Copyright",
publisher = "Frontiers Media SA",
journal = "Frontiers in Endocrinology",
title = "Nickel as a potential disruptor of thyroid function: benchmark modelling of human data",
volume = "14",
doi = "10.3389/fendo.2023.1145153"
}

Marić, Đ., Baralić, K., Javorac, D., Mandić-Rajčević, S., Žarković, M., Antonijević, B., Đukić-Ćosić, D., Bulat, Z.,& Buha-Đorđević, A.. (2023). Nickel as a potential disruptor of thyroid function: benchmark modelling of human data. in Frontiers in Endocrinology
Frontiers Media SA., 14.
https://doi.org/10.3389/fendo.2023.1145153

Marić Đ, Baralić K, Javorac D, Mandić-Rajčević S, Žarković M, Antonijević B, Đukić-Ćosić D, Bulat Z, Buha-Đorđević A. Nickel as a potential disruptor of thyroid function: benchmark modelling of human data. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1145153 .

Marić, Đurđica, Baralić, Katarina, Javorac, Dragana, Mandić-Rajčević, Stefan, Žarković, Miloš, Antonijević, Biljana, Đukić-Ćosić, Danijela, Bulat, Zorica, Buha-Đorđević, Aleksandra, "Nickel as a potential disruptor of thyroid function: benchmark modelling of human data" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1145153 . .

TY  - JOUR
AU  - Sarić-Matutinović, Marija
AU  - Kahaly, G.J.
AU  - Žarković, Miloš
AU  - Ćirić, J.
AU  - Ignjatović, Svetlana
AU  - Nedeljković Beleslin, B.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4654
AB  - Purpose: Graves’ orbitopathy (GO) is a specific inflammatory disorder of the orbit characterized by a highly heterogeneous clinical phenotype. The role of thyrotropin receptor antibodies (TSH-R-Ab) has been widely researched, however there is still no evidence that these antibodies have a direct pathogenic role in this pathology. The aim of this study was to examine their relation to the individual clinical features of GO. Methods: Ninety-one consecutive patients with GO were recruited. Total antibody concentration (TSH-R binding inhibitory immunoglobulins, TBII) and their functional activity (stimulating TSH-R-Ab, TSAb) were measured using binding immunoassay and cell-based bioassay, respectively. Results: Both TSAb and TBII levels were significantly associated to the clinical parameters of GO activity. TSAb was a more sensitive serological marker compared to TBII pertaining to eyelid retraction and edema, proptosis, extra-orbital muscle disorders, diplopia, irritable eye symptoms, and photophobia. TSAb, but not TBII, was a significant predictive marker of conjunctival redness, chemosis, caruncle/plica inflammation, eye irritation, and orbital pain, (odds ratio: 3.096, p = 0.016; 5.833, p = 0.009; 6.443, p = 0.020; 3.167, p = 0.045; 2.893, p = 0.032; versus 2.187, p = 0.093; 2.775, p = 0.081; 3.824, p = 0.055; 0.952, p = 0.930; 2.226, p = 0.099, respectively). Neither TSAb nor TBII correlated with the level of proptosis (ρ = 0.259, p = 0.090, and ρ = 0.254, p = 0.104, respectively), however rising TSAb levels were strongly associated to the level of proptosis. Conclusions: TSH-R-Ab were significantly associated with GO’s phenotype. Especially TSAb, as a sensitive and predictive serological biomarker, can improve diagnosis and management of GO.
PB  - Springer Science and Business Media
T2  - Journal of Endocrinological Investigation
T1  - The phenotype of Graves’ orbitopathy is associated with thyrotropin receptor antibody levels
DO  - 10.1007/s40618-023-02085-5
ER  - 

@article{
author = "Sarić-Matutinović, Marija and Kahaly, G.J. and Žarković, Miloš and Ćirić, J. and Ignjatović, Svetlana and Nedeljković Beleslin, B.",
year = "2023",
abstract = "Purpose: Graves’ orbitopathy (GO) is a specific inflammatory disorder of the orbit characterized by a highly heterogeneous clinical phenotype. The role of thyrotropin receptor antibodies (TSH-R-Ab) has been widely researched, however there is still no evidence that these antibodies have a direct pathogenic role in this pathology. The aim of this study was to examine their relation to the individual clinical features of GO. Methods: Ninety-one consecutive patients with GO were recruited. Total antibody concentration (TSH-R binding inhibitory immunoglobulins, TBII) and their functional activity (stimulating TSH-R-Ab, TSAb) were measured using binding immunoassay and cell-based bioassay, respectively. Results: Both TSAb and TBII levels were significantly associated to the clinical parameters of GO activity. TSAb was a more sensitive serological marker compared to TBII pertaining to eyelid retraction and edema, proptosis, extra-orbital muscle disorders, diplopia, irritable eye symptoms, and photophobia. TSAb, but not TBII, was a significant predictive marker of conjunctival redness, chemosis, caruncle/plica inflammation, eye irritation, and orbital pain, (odds ratio: 3.096, p = 0.016; 5.833, p = 0.009; 6.443, p = 0.020; 3.167, p = 0.045; 2.893, p = 0.032; versus 2.187, p = 0.093; 2.775, p = 0.081; 3.824, p = 0.055; 0.952, p = 0.930; 2.226, p = 0.099, respectively). Neither TSAb nor TBII correlated with the level of proptosis (ρ = 0.259, p = 0.090, and ρ = 0.254, p = 0.104, respectively), however rising TSAb levels were strongly associated to the level of proptosis. Conclusions: TSH-R-Ab were significantly associated with GO’s phenotype. Especially TSAb, as a sensitive and predictive serological biomarker, can improve diagnosis and management of GO.",
publisher = "Springer Science and Business Media",
journal = "Journal of Endocrinological Investigation",
title = "The phenotype of Graves’ orbitopathy is associated with thyrotropin receptor antibody levels",
doi = "10.1007/s40618-023-02085-5"
}

Sarić-Matutinović, M., Kahaly, G.J., Žarković, M., Ćirić, J., Ignjatović, S.,& Nedeljković Beleslin, B.. (2023). The phenotype of Graves’ orbitopathy is associated with thyrotropin receptor antibody levels. in Journal of Endocrinological Investigation
Springer Science and Business Media..
https://doi.org/10.1007/s40618-023-02085-5

Sarić-Matutinović M, Kahaly G, Žarković M, Ćirić J, Ignjatović S, Nedeljković Beleslin B. The phenotype of Graves’ orbitopathy is associated with thyrotropin receptor antibody levels. in Journal of Endocrinological Investigation. 2023;.
doi:10.1007/s40618-023-02085-5 .

Sarić-Matutinović, Marija, Kahaly, G.J., Žarković, Miloš, Ćirić, J., Ignjatović, Svetlana, Nedeljković Beleslin, B., "The phenotype of Graves’ orbitopathy is associated with thyrotropin receptor antibody levels" in Journal of Endocrinological Investigation (2023),
https://doi.org/10.1007/s40618-023-02085-5 . .

TY  - JOUR
AU  - Sarić-Matutinović, Marija
AU  - Diana, Tanja
AU  - Nedeljković-Beleslin, Biljana
AU  - Ćirić, Jasmina
AU  - Žarković, Miloš
AU  - Perović Blagojević, Iva
AU  - Kahaly, George
AU  - Ignjatović, Svetlana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4091
AB  - Background: Thyrotropin receptor autoantibodies (TSH-R-Ab) are indispensable biomarkers in the laboratory assessment of thyroid-associated orbitopathy (TAO). Clinical sensitivity of three different assays for TSH-R-Ab determination was evaluated in patients with TAO.

Methods: 87 consecutive TAO patients were enrolled and their serum samples analyzed in parallel with three assays. An ECLIA competitive binding and a chemiluminescent bridge immunoassay were used to measure total and bind-ing TSH-R-Ab concentration, while their functional activity was determined using a stimulatory TSH-R-Ab (TSAb) cell-based bioassay.

Results: Compared to the two binding assays (ECLIA p<0.001, bridge p=0.003), the TSAb bioassay was more sensitive pertaining to the positive detection of TSH-R-Ab in TAO patients. No difference (p=0.057) was noted between the ECLIA and bridge assays regarding sensitivity rate. All patients with active and/or moderate-to-severe TAO tested positive in the TSAb bioassay (100% and 100%, respectively), while the positivity rates for bridge and ECLIA binding assays were 89.7% and 82.1% for active TAO, and 90.2% and 86.3% for severe TAO, respectively. Negative predictive values of the bioassay, bridge, and ECLIA assays were 100%, 75%, and 71%, respectively for active TAO, and 100%, 86%, and 71%, respectively for moderate-to-severe TAO. The superiority of the bioassay was most prominent in euthyroid (ET) TAO. Positivity rates of the TSAb bioassay, bridge and ECLIA binding assays were 89.6%, 75%, and 64.6%, respectively for inactive TAO; 86.1%, 69.4%, and 52.8%, respectively for mild TAO; 87.5%, 62.5%, and 12.5%, respectively for euthyroid TAO. The bridge assay correlated better with the ECLIA binding assay (r=0.893, p<0.001), compared to the bioassay (r=0.669, p<0.001).

Conclusions: In patients with TAO of various activity and severity, the TSAb bioassay demonstrates a superior clinical performance compared to both ECLIA and bridge binding assays.
AB  - Uvod: Autoantitela na receptore za tireostimulišući hormon (TSH-R-Ab) su nezamenljivi biomarkeri u laboratorijskoj proceni orbitopatije udru'ene sa štitnom žlezdom (TAO). U radu je procenjena klinička osetljivost tri različita testa za određivanje TSH-R-Ab kod pacijenata sa TAO. Metode: U studiju je ključeno 87 uzastopnih pacijenata sa TAO i njihovi uzorci seruma su analizirani paralelno sa tri testa. Za merenje ukupne i vezujuće koncentracije TSH-RAb korišćeni su ECLIA imunohemijski test kompetitivnog vezivanja i hemiluminiscentni imunohemijski "sendvič" test, dok je njihova funkcionalna aktivnost određena pomoću ćelijskog biološkog testa (bioeseja) za određivanje stimulatornih TSH-R-Ab (TSAb). Rezultati: U poređenju sa dva imunohemijska testa vezivanja (ECLIA P<0,001, "sendvič test" P=0,003), TSAb bioesej se pokazao najosetljivijim u pogledu pozitivne detekcije TSH-RAb kod TAO pacijenata. Nikakva razlika nije detektovana (P=0,057) između ECLIA i "sendvič" testa u pogledu stope osetljivosti. TSAb bioesej je bio pozitivan kod svih pacijenata sa aktivnom i/ili umerenom do teškom TAO (redom 100% i 100%), dok su stope pozitivnosti za sendvič i ECLIA imunohemijski test bile 89,7% i 82,1% za aktivnu TAO, i 90,2% i 86,3% za tešku TAO, redom. Negativne prediktivne vrednosti bioeseja, "sendvič" i ECLIA testova bile su redom 100%, 75% i 71%, za aktivnu TAO, odnosno 100%, 86% i 71%, redom za umerenu do tešku TAO. Superiornost biološke analize bila je najistaknutija kod eutiroidnog oblika (ET) TAO. Stope pozitivnosti TSAb bioeseja, "sendvič" i ECLIA testova vezivanja bile su redom 89,6%, 75% i 64,6%, za neaktivnu TAO; 86,1%, 69,4% i 52,8% za blagu TAO; 87,5%, 62,5% i 12,5% za eutiroidni TAO. "Sendvič" imunohemijski test je bio u boljoj korelaciji sa ECLIA testom kompetitivnog vezivanja (r=0,893, P<0,001), u poređenju sa bioesejom (r=0,669, P<0,001). Zaključak: Kod pacijenata sa TAO različite aktivnosti i težine TSAb bioesej pokazuje superiorne kliničke performanse u poređenju sa obe vrste imunohemijskih testova vezivanja (ECLIA i "sendvič test").
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Sensitivity of three thyrotropin receptor antibody assays in thyroid-associated orbitopathy
T1  - Osetljivost tri testa za određivanje antitela na receptor za tireostimulišući hormon kod pacijenata sa orbitopatijom udruženom sa štitnom žlezdom
VL  - 41
IS  - 2
SP  - 211
EP  - 220
DO  - 10.5937/jomb0-34718
ER  - 

@article{
author = "Sarić-Matutinović, Marija and Diana, Tanja and Nedeljković-Beleslin, Biljana and Ćirić, Jasmina and Žarković, Miloš and Perović Blagojević, Iva and Kahaly, George and Ignjatović, Svetlana",
year = "2022",
abstract = "Background: Thyrotropin receptor autoantibodies (TSH-R-Ab) are indispensable biomarkers in the laboratory assessment of thyroid-associated orbitopathy (TAO). Clinical sensitivity of three different assays for TSH-R-Ab determination was evaluated in patients with TAO.

Methods: 87 consecutive TAO patients were enrolled and their serum samples analyzed in parallel with three assays. An ECLIA competitive binding and a chemiluminescent bridge immunoassay were used to measure total and bind-ing TSH-R-Ab concentration, while their functional activity was determined using a stimulatory TSH-R-Ab (TSAb) cell-based bioassay.

Results: Compared to the two binding assays (ECLIA p<0.001, bridge p=0.003), the TSAb bioassay was more sensitive pertaining to the positive detection of TSH-R-Ab in TAO patients. No difference (p=0.057) was noted between the ECLIA and bridge assays regarding sensitivity rate. All patients with active and/or moderate-to-severe TAO tested positive in the TSAb bioassay (100% and 100%, respectively), while the positivity rates for bridge and ECLIA binding assays were 89.7% and 82.1% for active TAO, and 90.2% and 86.3% for severe TAO, respectively. Negative predictive values of the bioassay, bridge, and ECLIA assays were 100%, 75%, and 71%, respectively for active TAO, and 100%, 86%, and 71%, respectively for moderate-to-severe TAO. The superiority of the bioassay was most prominent in euthyroid (ET) TAO. Positivity rates of the TSAb bioassay, bridge and ECLIA binding assays were 89.6%, 75%, and 64.6%, respectively for inactive TAO; 86.1%, 69.4%, and 52.8%, respectively for mild TAO; 87.5%, 62.5%, and 12.5%, respectively for euthyroid TAO. The bridge assay correlated better with the ECLIA binding assay (r=0.893, p<0.001), compared to the bioassay (r=0.669, p<0.001).

Conclusions: In patients with TAO of various activity and severity, the TSAb bioassay demonstrates a superior clinical performance compared to both ECLIA and bridge binding assays., Uvod: Autoantitela na receptore za tireostimulišući hormon (TSH-R-Ab) su nezamenljivi biomarkeri u laboratorijskoj proceni orbitopatije udru'ene sa štitnom žlezdom (TAO). U radu je procenjena klinička osetljivost tri različita testa za određivanje TSH-R-Ab kod pacijenata sa TAO. Metode: U studiju je ključeno 87 uzastopnih pacijenata sa TAO i njihovi uzorci seruma su analizirani paralelno sa tri testa. Za merenje ukupne i vezujuće koncentracije TSH-RAb korišćeni su ECLIA imunohemijski test kompetitivnog vezivanja i hemiluminiscentni imunohemijski "sendvič" test, dok je njihova funkcionalna aktivnost određena pomoću ćelijskog biološkog testa (bioeseja) za određivanje stimulatornih TSH-R-Ab (TSAb). Rezultati: U poređenju sa dva imunohemijska testa vezivanja (ECLIA P<0,001, "sendvič test" P=0,003), TSAb bioesej se pokazao najosetljivijim u pogledu pozitivne detekcije TSH-RAb kod TAO pacijenata. Nikakva razlika nije detektovana (P=0,057) između ECLIA i "sendvič" testa u pogledu stope osetljivosti. TSAb bioesej je bio pozitivan kod svih pacijenata sa aktivnom i/ili umerenom do teškom TAO (redom 100% i 100%), dok su stope pozitivnosti za sendvič i ECLIA imunohemijski test bile 89,7% i 82,1% za aktivnu TAO, i 90,2% i 86,3% za tešku TAO, redom. Negativne prediktivne vrednosti bioeseja, "sendvič" i ECLIA testova bile su redom 100%, 75% i 71%, za aktivnu TAO, odnosno 100%, 86% i 71%, redom za umerenu do tešku TAO. Superiornost biološke analize bila je najistaknutija kod eutiroidnog oblika (ET) TAO. Stope pozitivnosti TSAb bioeseja, "sendvič" i ECLIA testova vezivanja bile su redom 89,6%, 75% i 64,6%, za neaktivnu TAO; 86,1%, 69,4% i 52,8% za blagu TAO; 87,5%, 62,5% i 12,5% za eutiroidni TAO. "Sendvič" imunohemijski test je bio u boljoj korelaciji sa ECLIA testom kompetitivnog vezivanja (r=0,893, P<0,001), u poređenju sa bioesejom (r=0,669, P<0,001). Zaključak: Kod pacijenata sa TAO različite aktivnosti i težine TSAb bioesej pokazuje superiorne kliničke performanse u poređenju sa obe vrste imunohemijskih testova vezivanja (ECLIA i "sendvič test").",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Sensitivity of three thyrotropin receptor antibody assays in thyroid-associated orbitopathy, Osetljivost tri testa za određivanje antitela na receptor za tireostimulišući hormon kod pacijenata sa orbitopatijom udruženom sa štitnom žlezdom",
volume = "41",
number = "2",
pages = "211-220",
doi = "10.5937/jomb0-34718"
}

Sarić-Matutinović, M., Diana, T., Nedeljković-Beleslin, B., Ćirić, J., Žarković, M., Perović Blagojević, I., Kahaly, G.,& Ignjatović, S.. (2022). Sensitivity of three thyrotropin receptor antibody assays in thyroid-associated orbitopathy. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(2), 211-220.
https://doi.org/10.5937/jomb0-34718

Sarić-Matutinović M, Diana T, Nedeljković-Beleslin B, Ćirić J, Žarković M, Perović Blagojević I, Kahaly G, Ignjatović S. Sensitivity of three thyrotropin receptor antibody assays in thyroid-associated orbitopathy. in Journal of Medical Biochemistry. 2022;41(2):211-220.
doi:10.5937/jomb0-34718 .

Sarić-Matutinović, Marija, Diana, Tanja, Nedeljković-Beleslin, Biljana, Ćirić, Jasmina, Žarković, Miloš, Perović Blagojević, Iva, Kahaly, George, Ignjatović, Svetlana, "Sensitivity of three thyrotropin receptor antibody assays in thyroid-associated orbitopathy" in Journal of Medical Biochemistry, 41, no. 2 (2022):211-220,
https://doi.org/10.5937/jomb0-34718 . .

TY  - JOUR
AU  - Marić, Đurđica
AU  - Bonderović, Vera
AU  - Javorac, Dragana
AU  - Baralić, Katarina
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
AU  - Mandić-Rajčević, Stefan
AU  - Žarković, Miloš
AU  - Buha-Đorđević, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4400
AB  - Mercury (Hg) is one of the most important environmental pollutants with endocrine-
disrupting properties. There is little data from epidemiological studies describing the dose-
response relationship between toxic metal levels and hormone levels. The aim of this study was
to use the nearest neighbor matching analysis to determine the difference in Hg concentration in
healthy/sick subjects with thyroid disease and to use Benchmark modeling to determine the dose-
response relationship between Hg levels in the blood and thyroid-stimulating hormone (TSH) and
thyroid hormones in serum. Blood samples were collected and used for Hg measurement using
the ICP-MS method, and separated serum was used for hormone analysis. The study showed the
existence of a statistically significant difference in Hg levels measured in healthy and sick subjects
and the existence of a dose-response relationship between Hg and all measured hormones, with a
narrow interval obtained for the Hg-TSH pair. The results of this research support the use of the
Benchmark dose approach for the purpose of analyzing data from human studies, and our further
research will be focused on examining the impact of low doses on animal models in order to
determine more precise effects of low doses on the organism.
AB  - Živa (Hg) je jedan od najznačajnijih zagađivača životne sredine sa osobinama endokrinog ometača. Malo je podataka iz epidemioloških studija koji opisuju odnos doza-odgovor između nivoa toksičnih metala i nivoa hormona. Cilj ovog rada bio je da primenom nearest neighbor matching analize utvrdi razliku u koncentraciji Hg kod zdravih/obolelih ispitanika od bolesti štitaste žlezde i da primenom Benchmark modelovanja utvrdi odnos doza-odgovor između nivoa Hg u krvi i tireostimulišućeg hormona (TSH) i tiroidnih hormona u serumu. Uzorci krvi su sakupljeni i korišćeni za merenje Hg uz pomoć ICP-MS metode, a izdvojeni serum korišćen je za analizu hormona. Studija je pokazala postojanje statistički značajne razlike u nivoima Hg koji su izmereni kod zdravih i bolesnih ispitanika i postojanje odnosa doza-odgovor između Hg i svih merenih hormona, pri čemu je uzak interval dobijen za Hg-TSH par. Rezultati ovog istraživanja podržavaju upotrebu Benchmark dose pristupa u svrhu analize podataka iz humanih studija, a naša dalja istraživanja će biti usmerena na ispitivanje uticaja niskih doza na životinjskim modelima, u cilju utvrđivanja preciznijih efekata niskih doza na organizam.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Exposure to mercury and thyroid function: Is there a connection?
T1  - Izloženost živi i funkcija štitaste žlezde: postoji li veza?
VL  - 72
IS  - 5
SP  - 468
EP  - 485
DO  - 10.5937/arhfarm72-40122
ER  - 

@article{
author = "Marić, Đurđica and Bonderović, Vera and Javorac, Dragana and Baralić, Katarina and Bulat, Zorica and Đukić-Ćosić, Danijela and Mandić-Rajčević, Stefan and Žarković, Miloš and Buha-Đorđević, Aleksandra",
year = "2022",
abstract = "Mercury (Hg) is one of the most important environmental pollutants with endocrine-
disrupting properties. There is little data from epidemiological studies describing the dose-
response relationship between toxic metal levels and hormone levels. The aim of this study was
to use the nearest neighbor matching analysis to determine the difference in Hg concentration in
healthy/sick subjects with thyroid disease and to use Benchmark modeling to determine the dose-
response relationship between Hg levels in the blood and thyroid-stimulating hormone (TSH) and
thyroid hormones in serum. Blood samples were collected and used for Hg measurement using
the ICP-MS method, and separated serum was used for hormone analysis. The study showed the
existence of a statistically significant difference in Hg levels measured in healthy and sick subjects
and the existence of a dose-response relationship between Hg and all measured hormones, with a
narrow interval obtained for the Hg-TSH pair. The results of this research support the use of the
Benchmark dose approach for the purpose of analyzing data from human studies, and our further
research will be focused on examining the impact of low doses on animal models in order to
determine more precise effects of low doses on the organism., Živa (Hg) je jedan od najznačajnijih zagađivača životne sredine sa osobinama endokrinog ometača. Malo je podataka iz epidemioloških studija koji opisuju odnos doza-odgovor između nivoa toksičnih metala i nivoa hormona. Cilj ovog rada bio je da primenom nearest neighbor matching analize utvrdi razliku u koncentraciji Hg kod zdravih/obolelih ispitanika od bolesti štitaste žlezde i da primenom Benchmark modelovanja utvrdi odnos doza-odgovor između nivoa Hg u krvi i tireostimulišućeg hormona (TSH) i tiroidnih hormona u serumu. Uzorci krvi su sakupljeni i korišćeni za merenje Hg uz pomoć ICP-MS metode, a izdvojeni serum korišćen je za analizu hormona. Studija je pokazala postojanje statistički značajne razlike u nivoima Hg koji su izmereni kod zdravih i bolesnih ispitanika i postojanje odnosa doza-odgovor između Hg i svih merenih hormona, pri čemu je uzak interval dobijen za Hg-TSH par. Rezultati ovog istraživanja podržavaju upotrebu Benchmark dose pristupa u svrhu analize podataka iz humanih studija, a naša dalja istraživanja će biti usmerena na ispitivanje uticaja niskih doza na životinjskim modelima, u cilju utvrđivanja preciznijih efekata niskih doza na organizam.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Exposure to mercury and thyroid function: Is there a connection?, Izloženost živi i funkcija štitaste žlezde: postoji li veza?",
volume = "72",
number = "5",
pages = "468-485",
doi = "10.5937/arhfarm72-40122"
}

Marić, Đ., Bonderović, V., Javorac, D., Baralić, K., Bulat, Z., Đukić-Ćosić, D., Mandić-Rajčević, S., Žarković, M.,& Buha-Đorđević, A.. (2022). Exposure to mercury and thyroid function: Is there a connection?. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 72(5), 468-485.
https://doi.org/10.5937/arhfarm72-40122

Marić Đ, Bonderović V, Javorac D, Baralić K, Bulat Z, Đukić-Ćosić D, Mandić-Rajčević S, Žarković M, Buha-Đorđević A. Exposure to mercury and thyroid function: Is there a connection?. in Arhiv za farmaciju. 2022;72(5):468-485.
doi:10.5937/arhfarm72-40122 .

Marić, Đurđica, Bonderović, Vera, Javorac, Dragana, Baralić, Katarina, Bulat, Zorica, Đukić-Ćosić, Danijela, Mandić-Rajčević, Stefan, Žarković, Miloš, Buha-Đorđević, Aleksandra, "Exposure to mercury and thyroid function: Is there a connection?" in Arhiv za farmaciju, 72, no. 5 (2022):468-485,
https://doi.org/10.5937/arhfarm72-40122 . .

TY  - JOUR
AU  - Bećarević, Mirjana
AU  - Sarić-Matutinović, Marija
AU  - Žarković, Miloš
AU  - Nedeljković-Beleslin, Biljana
AU  - Ćirić, Jasmina
AU  - Ignjatović, Svetlana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3909
AB  - Purpose: Graves’ orbitopathy (GO) is an inflammatory autoimmune disorder of the orbit and while the antiphospholipid antibodies (aPL) Abs were associated with the markers of inflammation in the antiphospholipid syndrome (APS), there is no literature that investigate the presence of aPL Abs in GO. We analyzed the prevalence of aPL Abs and the differences between aPL (+) and aPL (−) subgroups of GO patients. Methods: Study included consecutive patients with GO (66 with Graves’ (GD), 10 with Hashimoto (HD), and 8 were euthyroid). Anticardiolipin (aCL) and anti-beta 2glycoprotein I (aβ2gpI) Abs were measured by ELISA. Results: aPL Abs were present in 9/84 (10.71%) patients. The IgM aβ2gpI Abs were present in 8/66 and in 1/10 patients with GD and HD. The IgG aCL Abs were present in one GD patient, and IgM aCL were present in 3/66 GD and in 1/10 patients with HD. In GD group, anti-Tg Abs were in positive correlation with aβ2gpI IgG (p = 0.000) and with anti-TPO Abs (p = 0.016). In HD group, anti-Tg Abs were in positive correlation with IgM aCL (p = 0.042), while anti-TPO Abs were in positive correlation with aβ2gpI IgM (p = 0.014). Conclusion: This study is the first report of the aPL Abs presence in GO patients. The anti-thyroid Abs were linked to aPL suggesting that their presence is not the sole consequence of hyperstimulation of autoreactive B-lymphocytes. Larger studies are necessary to confirm potential cause-effect relations. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PB  - Springer
T2  - Endocrine
T1  - Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data
DO  - 10.1007/s12020-021-02769-z
ER  - 

@article{
author = "Bećarević, Mirjana and Sarić-Matutinović, Marija and Žarković, Miloš and Nedeljković-Beleslin, Biljana and Ćirić, Jasmina and Ignjatović, Svetlana",
year = "2021",
abstract = "Purpose: Graves’ orbitopathy (GO) is an inflammatory autoimmune disorder of the orbit and while the antiphospholipid antibodies (aPL) Abs were associated with the markers of inflammation in the antiphospholipid syndrome (APS), there is no literature that investigate the presence of aPL Abs in GO. We analyzed the prevalence of aPL Abs and the differences between aPL (+) and aPL (−) subgroups of GO patients. Methods: Study included consecutive patients with GO (66 with Graves’ (GD), 10 with Hashimoto (HD), and 8 were euthyroid). Anticardiolipin (aCL) and anti-beta 2glycoprotein I (aβ2gpI) Abs were measured by ELISA. Results: aPL Abs were present in 9/84 (10.71%) patients. The IgM aβ2gpI Abs were present in 8/66 and in 1/10 patients with GD and HD. The IgG aCL Abs were present in one GD patient, and IgM aCL were present in 3/66 GD and in 1/10 patients with HD. In GD group, anti-Tg Abs were in positive correlation with aβ2gpI IgG (p = 0.000) and with anti-TPO Abs (p = 0.016). In HD group, anti-Tg Abs were in positive correlation with IgM aCL (p = 0.042), while anti-TPO Abs were in positive correlation with aβ2gpI IgM (p = 0.014). Conclusion: This study is the first report of the aPL Abs presence in GO patients. The anti-thyroid Abs were linked to aPL suggesting that their presence is not the sole consequence of hyperstimulation of autoreactive B-lymphocytes. Larger studies are necessary to confirm potential cause-effect relations. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
publisher = "Springer",
journal = "Endocrine",
title = "Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data",
doi = "10.1007/s12020-021-02769-z"
}

Bećarević, M., Sarić-Matutinović, M., Žarković, M., Nedeljković-Beleslin, B., Ćirić, J.,& Ignjatović, S.. (2021). Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data. in Endocrine
Springer..
https://doi.org/10.1007/s12020-021-02769-z

Bećarević M, Sarić-Matutinović M, Žarković M, Nedeljković-Beleslin B, Ćirić J, Ignjatović S. Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data. in Endocrine. 2021;.
doi:10.1007/s12020-021-02769-z .

Bećarević, Mirjana, Sarić-Matutinović, Marija, Žarković, Miloš, Nedeljković-Beleslin, Biljana, Ćirić, Jasmina, Ignjatović, Svetlana, "Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data" in Endocrine (2021),
https://doi.org/10.1007/s12020-021-02769-z . .

TY  - JOUR
AU  - Radosavljević, Branimir
AU  - Žarković, Miloš
AU  - Ignjatović, Svetlana
AU  - Dajak, Marijana
AU  - Milinković, Neda
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2700
AB  - Physical effort is known to alter the blood levels of many hormones, but there are only a few studies about the biological changes of salivary hormones. The aim of this work was to determine whether salivary testosterone and salivary cortisol levels, measured two weeks before a half-marathon race, relate to running performance in male recreational athletes. A group of eleven male recreational athletes preparing for a half-marathon was included in the study. Saliva for testosterone and cortisol determinations was collected before and immediately after a 15-km training run, two weeks before the half-marathon. Individual official half-marathon times, expressed in hours, were used as a measure of performance. Mean testosterone concentrations were 1.07 +/- 0.33 nmol/l before the run and 0.88 +/- 0.35 nmol/l after the run (p lt 0.05). Mean cortisol concentrations were 12.28 +/- 8.46 nmol/l before the run and 38.08 +/- 19.63 nmol/l after the run (p lt 0.05). The pre-run salivary testosterone levels marginally correlated with the corresponding half-marathon running times (p=0.068, 95% bootstrap CI for slope -0.40 to -0.06). However, post-run salivary testosterone levels significantly correlated with the corresponding half-marathon running times (p=0.011, 95% bootstrap CI for slope -0.41 to -0.16), even considering correlations with the runners' age. Salivary cortisol levels, either pre- or post-run, did not correlate with the corresponding half-marathon running times. The results of this study suggest that post-exercise salivary testosterone levels could have the potential to predict performance in endurance running, at least in recreational athletes.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Biological aspects of salivary hormones in male half-marathon performance
VL  - 68
IS  - 3
SP  - 495
EP  - 500
DO  - 10.2298/ABS150904038R
ER  - 

@article{
author = "Radosavljević, Branimir and Žarković, Miloš and Ignjatović, Svetlana and Dajak, Marijana and Milinković, Neda",
year = "2016",
abstract = "Physical effort is known to alter the blood levels of many hormones, but there are only a few studies about the biological changes of salivary hormones. The aim of this work was to determine whether salivary testosterone and salivary cortisol levels, measured two weeks before a half-marathon race, relate to running performance in male recreational athletes. A group of eleven male recreational athletes preparing for a half-marathon was included in the study. Saliva for testosterone and cortisol determinations was collected before and immediately after a 15-km training run, two weeks before the half-marathon. Individual official half-marathon times, expressed in hours, were used as a measure of performance. Mean testosterone concentrations were 1.07 +/- 0.33 nmol/l before the run and 0.88 +/- 0.35 nmol/l after the run (p lt 0.05). Mean cortisol concentrations were 12.28 +/- 8.46 nmol/l before the run and 38.08 +/- 19.63 nmol/l after the run (p lt 0.05). The pre-run salivary testosterone levels marginally correlated with the corresponding half-marathon running times (p=0.068, 95% bootstrap CI for slope -0.40 to -0.06). However, post-run salivary testosterone levels significantly correlated with the corresponding half-marathon running times (p=0.011, 95% bootstrap CI for slope -0.41 to -0.16), even considering correlations with the runners' age. Salivary cortisol levels, either pre- or post-run, did not correlate with the corresponding half-marathon running times. The results of this study suggest that post-exercise salivary testosterone levels could have the potential to predict performance in endurance running, at least in recreational athletes.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Biological aspects of salivary hormones in male half-marathon performance",
volume = "68",
number = "3",
pages = "495-500",
doi = "10.2298/ABS150904038R"
}

Radosavljević, B., Žarković, M., Ignjatović, S., Dajak, M.,& Milinković, N.. (2016). Biological aspects of salivary hormones in male half-marathon performance. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(3), 495-500.
https://doi.org/10.2298/ABS150904038R

Radosavljević B, Žarković M, Ignjatović S, Dajak M, Milinković N. Biological aspects of salivary hormones in male half-marathon performance. in Archives of Biological Sciences. 2016;68(3):495-500.
doi:10.2298/ABS150904038R .

Radosavljević, Branimir, Žarković, Miloš, Ignjatović, Svetlana, Dajak, Marijana, Milinković, Neda, "Biological aspects of salivary hormones in male half-marathon performance" in Archives of Biological Sciences, 68, no. 3 (2016):495-500,
https://doi.org/10.2298/ABS150904038R . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Jovičić, Snežana
AU  - Radosavljević, Branimir
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2099
AB  - Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results
VL  - 74
IS  - 5
SP  - 378
EP  - 384
DO  - 10.3109/00365513.2014.898324
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Jovičić, Snežana and Radosavljević, Branimir and Singh, Sandra and Majkić-Singh, Nada",
year = "2014",
abstract = "Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results",
volume = "74",
number = "5",
pages = "378-384",
doi = "10.3109/00365513.2014.898324"
}

Milinković, N., Ignjatović, S., Žarković, M., Jovičić, S., Radosavljević, B., Singh, S.,& Majkić-Singh, N.. (2014). Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 74(5), 378-384.
https://doi.org/10.3109/00365513.2014.898324

Milinković N, Ignjatović S, Žarković M, Jovičić S, Radosavljević B, Singh S, Majkić-Singh N. Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation. 2014;74(5):378-384.
doi:10.3109/00365513.2014.898324 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Jovičić, Snežana, Radosavljević, Branimir, Singh, Sandra, Majkić-Singh, Nada, "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results" in Scandinavian Journal of Clinical and Laboratory Investigation, 74, no. 5 (2014):378-384,
https://doi.org/10.3109/00365513.2014.898324 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Radosavljević, Branimir
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2081
AB  - Background: The aim of this work was to determine indirect reference intervals from patients' results obtained during routine laboratory work. This could be an accurate alternative to the laborious and expensive job of producing reference intervals for populations according to international recommendations. Methods: All the results for thyrotropin (TSH), total and free thyroxine, and triiodothyronine (T4, fT4, T3, and fT3) stored in our laboratory information system between 2008 and 2011 were included in this study. We used logarithmic transformation of the raw data to exclude outliers. After visual observation of the data distribution, we estimated non-parametric reference intervals. A standard normal deviation test was performed to test the significance of differences between subgroups. Results: There was no significant difference in the serum levels of the analyzed thyroid parameters, so we calculated combined reference values. However, we found a significant difference in TSH values between ambulatory and hospitalized patients, but only in 2011. Indirect reference values for TSH, T4, fT4, T3 and fT3 were 0.42 - 3.67 mIU/L, 66.0 - 136.10 nmol/L, 10.20 - 18.40 pmol/L, 1.10 - 2.39 nmol/L, and 3.17 - 5.59 pmol/L, respectively. Conclusions: The indirect determination of laboratory-specific reference intervals using patients' laboratory data is a relatively easy and inexpensive method. Also, indirect reference limits will be more precise and true if skewness and kurtosis of the distribution are not too large.
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Indirect Estimation of Reference Intervals for Thyroid Parameters
VL  - 60
IS  - 7
SP  - 1083
EP  - 1089
DO  - 10.7754/Clin.Lab.2013.130733
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Radosavljević, Branimir and Majkić-Singh, Nada",
year = "2014",
abstract = "Background: The aim of this work was to determine indirect reference intervals from patients' results obtained during routine laboratory work. This could be an accurate alternative to the laborious and expensive job of producing reference intervals for populations according to international recommendations. Methods: All the results for thyrotropin (TSH), total and free thyroxine, and triiodothyronine (T4, fT4, T3, and fT3) stored in our laboratory information system between 2008 and 2011 were included in this study. We used logarithmic transformation of the raw data to exclude outliers. After visual observation of the data distribution, we estimated non-parametric reference intervals. A standard normal deviation test was performed to test the significance of differences between subgroups. Results: There was no significant difference in the serum levels of the analyzed thyroid parameters, so we calculated combined reference values. However, we found a significant difference in TSH values between ambulatory and hospitalized patients, but only in 2011. Indirect reference values for TSH, T4, fT4, T3 and fT3 were 0.42 - 3.67 mIU/L, 66.0 - 136.10 nmol/L, 10.20 - 18.40 pmol/L, 1.10 - 2.39 nmol/L, and 3.17 - 5.59 pmol/L, respectively. Conclusions: The indirect determination of laboratory-specific reference intervals using patients' laboratory data is a relatively easy and inexpensive method. Also, indirect reference limits will be more precise and true if skewness and kurtosis of the distribution are not too large.",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Indirect Estimation of Reference Intervals for Thyroid Parameters",
volume = "60",
number = "7",
pages = "1083-1089",
doi = "10.7754/Clin.Lab.2013.130733"
}

Milinković, N., Ignjatović, S., Žarković, M., Radosavljević, B.,& Majkić-Singh, N.. (2014). Indirect Estimation of Reference Intervals for Thyroid Parameters. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 60(7), 1083-1089.
https://doi.org/10.7754/Clin.Lab.2013.130733

Milinković N, Ignjatović S, Žarković M, Radosavljević B, Majkić-Singh N. Indirect Estimation of Reference Intervals for Thyroid Parameters. in Clinical Laboratory. 2014;60(7):1083-1089.
doi:10.7754/Clin.Lab.2013.130733 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Radosavljević, Branimir, Majkić-Singh, Nada, "Indirect Estimation of Reference Intervals for Thyroid Parameters" in Clinical Laboratory, 60, no. 7 (2014):1083-1089,
https://doi.org/10.7754/Clin.Lab.2013.130733 . .

TY  - JOUR
AU  - Žarković, Miloš
AU  - Ignjatović, Svetlana
AU  - Dajak, Marijana
AU  - Ćirić, Jasmina
AU  - Beleslin, Biljana
AU  - Savić, Slavica
AU  - Stojković, Mirjana
AU  - Bulat, Petar
AU  - Trbojević, Bozo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1026
AB  - Objective: Interleukin 6(IL6) has the ability to influence each level of the hypothalamo-pituitary-adrenocortical (HPA) axis. The aim of the study was to test whether IL6 concentration correlates with the adrenal cortex response to ACTH in healthy humans. We postulated that higher basal IL6 concentration would be associated with the higher cortisol response to the stimulation. Design and methods: Basal IL6 concentration was measured and a low dose (1 mu g) ACTH test was performed to assess cortisol response. apparently healthy Subjects (11 male. 16 female. mean age 31.1 years. age range 22-47 years) were included in the study. Results: Data are presented as mean +/- S.E.M. Basal IL6 level was 0.84 +/- 0.10 pg/ml, Basal cortisol was 351.9 +/- 18.3. nmol/l. Maximal cortisol during synacthen test was 653.0 +/- 20.6 nmol/l. Maximal cortisol increment was 301.1 +/- 20.0 nmol/l. IL6 concentration was not correlated with basal or maximal cortisol concentration. but correlated significantly with cortisol increment (r=0.63. 95%. confidence interval) 0.42-0.83). Conclusions: In our study. We found that higher basal IL6 concentration is associated with the higher cortisol response to ACTH stimulation. Based on previous research and our data, IL6, even in low concentrations and under physiologic conditions, modulates adrenal cortex responsivity to ACTH. Therefore. it seems that immune modulation of HPA axis is also present under physiologic and not only pathologic conditions.
PB  - Bioscientifica Ltd, Bristol
T2  - European Journal of Endocrinology
T1  - Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans
VL  - 159
IS  - 5
SP  - 649
EP  - 652
DO  - 10.1530/EJE-08-0544
ER  - 

@article{
author = "Žarković, Miloš and Ignjatović, Svetlana and Dajak, Marijana and Ćirić, Jasmina and Beleslin, Biljana and Savić, Slavica and Stojković, Mirjana and Bulat, Petar and Trbojević, Bozo",
year = "2008",
abstract = "Objective: Interleukin 6(IL6) has the ability to influence each level of the hypothalamo-pituitary-adrenocortical (HPA) axis. The aim of the study was to test whether IL6 concentration correlates with the adrenal cortex response to ACTH in healthy humans. We postulated that higher basal IL6 concentration would be associated with the higher cortisol response to the stimulation. Design and methods: Basal IL6 concentration was measured and a low dose (1 mu g) ACTH test was performed to assess cortisol response. apparently healthy Subjects (11 male. 16 female. mean age 31.1 years. age range 22-47 years) were included in the study. Results: Data are presented as mean +/- S.E.M. Basal IL6 level was 0.84 +/- 0.10 pg/ml, Basal cortisol was 351.9 +/- 18.3. nmol/l. Maximal cortisol during synacthen test was 653.0 +/- 20.6 nmol/l. Maximal cortisol increment was 301.1 +/- 20.0 nmol/l. IL6 concentration was not correlated with basal or maximal cortisol concentration. but correlated significantly with cortisol increment (r=0.63. 95%. confidence interval) 0.42-0.83). Conclusions: In our study. We found that higher basal IL6 concentration is associated with the higher cortisol response to ACTH stimulation. Based on previous research and our data, IL6, even in low concentrations and under physiologic conditions, modulates adrenal cortex responsivity to ACTH. Therefore. it seems that immune modulation of HPA axis is also present under physiologic and not only pathologic conditions.",
publisher = "Bioscientifica Ltd, Bristol",
journal = "European Journal of Endocrinology",
title = "Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans",
volume = "159",
number = "5",
pages = "649-652",
doi = "10.1530/EJE-08-0544"
}

Žarković, M., Ignjatović, S., Dajak, M., Ćirić, J., Beleslin, B., Savić, S., Stojković, M., Bulat, P.,& Trbojević, B.. (2008). Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans. in European Journal of Endocrinology
Bioscientifica Ltd, Bristol., 159(5), 649-652.
https://doi.org/10.1530/EJE-08-0544

Žarković M, Ignjatović S, Dajak M, Ćirić J, Beleslin B, Savić S, Stojković M, Bulat P, Trbojević B. Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans. in European Journal of Endocrinology. 2008;159(5):649-652.
doi:10.1530/EJE-08-0544 .

Žarković, Miloš, Ignjatović, Svetlana, Dajak, Marijana, Ćirić, Jasmina, Beleslin, Biljana, Savić, Slavica, Stojković, Mirjana, Bulat, Petar, Trbojević, Bozo, "Cortisol response to ACTH stimulation correlates with blood interleukin 6 concentration in healthy humans" in European Journal of Endocrinology, 159, no. 5 (2008):649-652,
https://doi.org/10.1530/EJE-08-0544 . .