TY  - JOUR
AU  - Lippi, Giuseppe
AU  - Cadamuro, Janne
AU  - Danese, Elisa
AU  - Favaloro, Emmanuel J.
AU  - Favresse, Julien
AU  - Henry, Brandon M.
AU  - Jovičić, Snežana
AU  - Ozben, Tomris
AU  - Plebani, Mario
AU  - Thachil, Jecko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5343
AB  - Background: The EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) created a survey that has been distributed to its members for gathering information on the key hazards experienced by European medical laboratories during the COVID-19 pandemic. Methods: The survey was distributed to over 12,000 potential contacts (laboratory workers) via an EFLM newsletter, with responses collected between May 8 and June 8, 2023. Results: Two hundred replies were collected and examined from European laboratories. 69.7% and 78.1% of all responders said they were short on non-COVID and COVID reagents, respectively. Exactly half of respondents (50.0%) said that they could not complete all laboratory tests required for a specific period, but this figure climbed to 61.2% for COVID tests. Finally, 72.3% of respondents expressed exhaustion during the pandemic, and 61.2% reported increasing patient hostility. Conclusions: The COVID-19 pandemic had a significant impact on laboratory medicine in Europe. Cultural change, proactive planning, and even re-engineering in some parts of the laboratory industry may thus be necessary to prepare for future challenges.
PB  - International Federation of Clinical Chemistry and Laboratory Medicine
T2  - Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine
T1  - Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey
VL  - 34
IS  - 3
SP  - 213
EP  - 219
DO  - 10.20944/preprints202306.1592.v1
ER  - 

@article{
author = "Lippi, Giuseppe and Cadamuro, Janne and Danese, Elisa and Favaloro, Emmanuel J. and Favresse, Julien and Henry, Brandon M. and Jovičić, Snežana and Ozben, Tomris and Plebani, Mario and Thachil, Jecko",
year = "2023",
abstract = "Background: The EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) created a survey that has been distributed to its members for gathering information on the key hazards experienced by European medical laboratories during the COVID-19 pandemic. Methods: The survey was distributed to over 12,000 potential contacts (laboratory workers) via an EFLM newsletter, with responses collected between May 8 and June 8, 2023. Results: Two hundred replies were collected and examined from European laboratories. 69.7% and 78.1% of all responders said they were short on non-COVID and COVID reagents, respectively. Exactly half of respondents (50.0%) said that they could not complete all laboratory tests required for a specific period, but this figure climbed to 61.2% for COVID tests. Finally, 72.3% of respondents expressed exhaustion during the pandemic, and 61.2% reported increasing patient hostility. Conclusions: The COVID-19 pandemic had a significant impact on laboratory medicine in Europe. Cultural change, proactive planning, and even re-engineering in some parts of the laboratory industry may thus be necessary to prepare for future challenges.",
publisher = "International Federation of Clinical Chemistry and Laboratory Medicine",
journal = "Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine",
title = "Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey",
volume = "34",
number = "3",
pages = "213-219",
doi = "10.20944/preprints202306.1592.v1"
}

Lippi, G., Cadamuro, J., Danese, E., Favaloro, E. J., Favresse, J., Henry, B. M., Jovičić, S., Ozben, T., Plebani, M.,& Thachil, J.. (2023). Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey. in Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine
International Federation of Clinical Chemistry and Laboratory Medicine., 34(3), 213-219.
https://doi.org/10.20944/preprints202306.1592.v1

Lippi G, Cadamuro J, Danese E, Favaloro EJ, Favresse J, Henry BM, Jovičić S, Ozben T, Plebani M, Thachil J. Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey. in Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. 2023;34(3):213-219.
doi:10.20944/preprints202306.1592.v1 .

Lippi, Giuseppe, Cadamuro, Janne, Danese, Elisa, Favaloro, Emmanuel J., Favresse, Julien, Henry, Brandon M., Jovičić, Snežana, Ozben, Tomris, Plebani, Mario, Thachil, Jecko, "Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey" in Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine, 34, no. 3 (2023):213-219,
https://doi.org/10.20944/preprints202306.1592.v1 . .

TY  - JOUR
AU  - Lippi, Giuseppe
AU  - Cadamuro, Janne
AU  - Danese, Elisa
AU  - Favaloro, Emmanuel J.
AU  - Favresse, Julien
AU  - Henry, Brandon M.
AU  - Jovičić, Snežana
AU  - Ozben, Tomris
AU  - Plebani, Mario
AU  - Thachil, Jecko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4935
PB  - De Gruyter Open Ltd
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Results of the first survey of the EFLM Task Force Preparation of Labs for Emergencies (TF-PLE)
DO  - 10.1515/cclm-2023-0622
ER  - 

@article{
author = "Lippi, Giuseppe and Cadamuro, Janne and Danese, Elisa and Favaloro, Emmanuel J. and Favresse, Julien and Henry, Brandon M. and Jovičić, Snežana and Ozben, Tomris and Plebani, Mario and Thachil, Jecko",
year = "2023",
publisher = "De Gruyter Open Ltd",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Results of the first survey of the EFLM Task Force Preparation of Labs for Emergencies (TF-PLE)",
doi = "10.1515/cclm-2023-0622"
}

Lippi, G., Cadamuro, J., Danese, E., Favaloro, E. J., Favresse, J., Henry, B. M., Jovičić, S., Ozben, T., Plebani, M.,& Thachil, J.. (2023). Results of the first survey of the EFLM Task Force Preparation of Labs for Emergencies (TF-PLE). in Clinical Chemistry and Laboratory Medicine
De Gruyter Open Ltd..
https://doi.org/10.1515/cclm-2023-0622

Lippi G, Cadamuro J, Danese E, Favaloro EJ, Favresse J, Henry BM, Jovičić S, Ozben T, Plebani M, Thachil J. Results of the first survey of the EFLM Task Force Preparation of Labs for Emergencies (TF-PLE). in Clinical Chemistry and Laboratory Medicine. 2023;.
doi:10.1515/cclm-2023-0622 .

Lippi, Giuseppe, Cadamuro, Janne, Danese, Elisa, Favaloro, Emmanuel J., Favresse, Julien, Henry, Brandon M., Jovičić, Snežana, Ozben, Tomris, Plebani, Mario, Thachil, Jecko, "Results of the first survey of the EFLM Task Force Preparation of Labs for Emergencies (TF-PLE)" in Clinical Chemistry and Laboratory Medicine (2023),
https://doi.org/10.1515/cclm-2023-0622 . .

TY  - JOUR
AU  - Vicković, Sanja
AU  - Zdravković, Ranko
AU  - Maričić-Prijić, Sanja
AU  - Nikolić, Dragan
AU  - Pap, Dragana
AU  - Čolak, Emina
AU  - Jovičić, Snežana
PY  - 2023
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151461118&doi=10.5937%2fjomb0-42011&partnerID=40&md5=e093411634659d1acbf5cdbb88450218
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4655
AB  - Background: Surgical stress and pain result in activation of hypothalamus-pituitary-adrenal axis. The aim of this study was to establish the effects of postoperative pain and various modalities of analgesic administration on salivary and serum cortisol levels, as well as to establish the validity of salivary cortisol as a stress indicator in surgical patients. Methods: A randomized controlled trial involved 60 patients scheduled for elective abdominal aortic aneurysm surgery. Patients were randomly divided into two groups depending on the model of postoperative analgesia. The first group (MI – morphine intermittently) included patients given morphine doses 0.1 mg/kg/6h s.c. intermittently. The second group (MPCA – morphine patient-controlled analgesia) included patients who received morphine via the PCA system – intravenous administration of morphine adjusted to a dose of 1 mg per shot and a lockout interval of 6 minutes. Results: The intensity of pain did not significantly vary until the hour 10 post-surgery. However, in the period from hour 10 to hour 18 post-surgery, higher intensity of pain was reported in group MPCA (P < 0.05). Hemodynamic instability was more prevalent in the MI group (40.0% vs 6.7%, P = 0.0048). Serum cortisol levels were almost identical in both groups (MI 509.4 nmol/L vs MPCA 511.0 nmol/L, P = 0.1473). Higher values of salivary cortisol were recorded in group MPCA; however, the difference was not statistically significant (47.1 nmol/L vs 116.3 nmol/L, P = 0.0970). Conclusion: Our study confirmed that salivary cortisol is a more sensitive stress biomarker in surgical patients as compared to blood cortisol.
AB  - Uvod: Hirur{ki stres i bol su uzrok aktivacije hipotalamus- no-hipofizno-nadbubre`ne osovine. Cilj ove studije bio je da se utvrdi uticaj postoperativnog bola i razli~itih vidova administracije analgetika na vrednosti kortizola u serumu i salivi, kao i da se utvrdi da li je salivarni kortizol dobar poka- zatelj stresa kod hirur{kih pacijenata. Metode: Randomizovana kontrolisana studija koja je uklju- ~ila 60 pacijenata primljenih za elektivnu operaciju ane- urizme abdominalne aorte. Pacijenti su metodom slu~ajnog izbora podeljeni u dve grupe u zavisnosti od modela post- operativne analgezije. Prvu grupu (MI – intermitentna pri- mena morfina) su ~inili pacijenti koji su dobijali morfin intermitentno u dozi od 0.1 mg/kg/6h s.c. U drugoj grupi (MPCA – analgezija morfinom kontrolisana od strane paci- jenta) su bili pacijenti koji su dobijali morfin takozvanom PCA metodom – intravenska primena morfina 1 pritisak/1 mg, interval 6 minuta. Rezultati: Intenzitet bola se nije zna~ajno razlikovao do desetog sata nakon operacije. Me|utim, u periodu od de- setog do osamnaestog sata nakon operacije bol je bio izra`eniji u MPCA grupi (P < 0.05). Hemodinamska nesta- bilnost je bila zastupljenija u grupi MI (40.0% vs 6.7%, P = 0.0048). Serumski kortizol bio je gotovo identi~an po gru- pama (MI 509.4 nmol/L vs MPCA 511.0 nmol/L, P = 0.1473). Salivarni kortizol je bio vi{i u grupi MPCA ali razika nije statisti~ki zna~ajna (47.1 nmol/L vs 116.3 nmol/L, P = 0.0970). Zaklju~ak: Na{a studija je pokazala da je salivarni kortizol senzitivniji biomarker stresa kod hirur{kih pacijenata u odnosu na serumski kortizol.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Salivary cortisol as a biomarker of stress in surgical patients
T1  - Salivarni kortizol kao biomarker stresa kod hirurških pacijenata
VL  - 42
SP  - 1
EP  - 7
DO  - 10.5937/jomb0-42011
ER  - 

@article{
author = "Vicković, Sanja and Zdravković, Ranko and Maričić-Prijić, Sanja and Nikolić, Dragan and Pap, Dragana and Čolak, Emina and Jovičić, Snežana",
year = "2023",
abstract = "Background: Surgical stress and pain result in activation of hypothalamus-pituitary-adrenal axis. The aim of this study was to establish the effects of postoperative pain and various modalities of analgesic administration on salivary and serum cortisol levels, as well as to establish the validity of salivary cortisol as a stress indicator in surgical patients. Methods: A randomized controlled trial involved 60 patients scheduled for elective abdominal aortic aneurysm surgery. Patients were randomly divided into two groups depending on the model of postoperative analgesia. The first group (MI – morphine intermittently) included patients given morphine doses 0.1 mg/kg/6h s.c. intermittently. The second group (MPCA – morphine patient-controlled analgesia) included patients who received morphine via the PCA system – intravenous administration of morphine adjusted to a dose of 1 mg per shot and a lockout interval of 6 minutes. Results: The intensity of pain did not significantly vary until the hour 10 post-surgery. However, in the period from hour 10 to hour 18 post-surgery, higher intensity of pain was reported in group MPCA (P < 0.05). Hemodynamic instability was more prevalent in the MI group (40.0% vs 6.7%, P = 0.0048). Serum cortisol levels were almost identical in both groups (MI 509.4 nmol/L vs MPCA 511.0 nmol/L, P = 0.1473). Higher values of salivary cortisol were recorded in group MPCA; however, the difference was not statistically significant (47.1 nmol/L vs 116.3 nmol/L, P = 0.0970). Conclusion: Our study confirmed that salivary cortisol is a more sensitive stress biomarker in surgical patients as compared to blood cortisol., Uvod: Hirur{ki stres i bol su uzrok aktivacije hipotalamus- no-hipofizno-nadbubre`ne osovine. Cilj ove studije bio je da se utvrdi uticaj postoperativnog bola i razli~itih vidova administracije analgetika na vrednosti kortizola u serumu i salivi, kao i da se utvrdi da li je salivarni kortizol dobar poka- zatelj stresa kod hirur{kih pacijenata. Metode: Randomizovana kontrolisana studija koja je uklju- ~ila 60 pacijenata primljenih za elektivnu operaciju ane- urizme abdominalne aorte. Pacijenti su metodom slu~ajnog izbora podeljeni u dve grupe u zavisnosti od modela post- operativne analgezije. Prvu grupu (MI – intermitentna pri- mena morfina) su ~inili pacijenti koji su dobijali morfin intermitentno u dozi od 0.1 mg/kg/6h s.c. U drugoj grupi (MPCA – analgezija morfinom kontrolisana od strane paci- jenta) su bili pacijenti koji su dobijali morfin takozvanom PCA metodom – intravenska primena morfina 1 pritisak/1 mg, interval 6 minuta. Rezultati: Intenzitet bola se nije zna~ajno razlikovao do desetog sata nakon operacije. Me|utim, u periodu od de- setog do osamnaestog sata nakon operacije bol je bio izra`eniji u MPCA grupi (P < 0.05). Hemodinamska nesta- bilnost je bila zastupljenija u grupi MI (40.0% vs 6.7%, P = 0.0048). Serumski kortizol bio je gotovo identi~an po gru- pama (MI 509.4 nmol/L vs MPCA 511.0 nmol/L, P = 0.1473). Salivarni kortizol je bio vi{i u grupi MPCA ali razika nije statisti~ki zna~ajna (47.1 nmol/L vs 116.3 nmol/L, P = 0.0970). Zaklju~ak: Na{a studija je pokazala da je salivarni kortizol senzitivniji biomarker stresa kod hirur{kih pacijenata u odnosu na serumski kortizol.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Salivary cortisol as a biomarker of stress in surgical patients, Salivarni kortizol kao biomarker stresa kod hirurških pacijenata",
volume = "42",
pages = "1-7",
doi = "10.5937/jomb0-42011"
}

Vicković, S., Zdravković, R., Maričić-Prijić, S., Nikolić, D., Pap, D., Čolak, E.,& Jovičić, S.. (2023). Salivary cortisol as a biomarker of stress in surgical patients. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 42, 1-7.
https://doi.org/10.5937/jomb0-42011

Vicković S, Zdravković R, Maričić-Prijić S, Nikolić D, Pap D, Čolak E, Jovičić S. Salivary cortisol as a biomarker of stress in surgical patients. in Journal of Medical Biochemistry. 2023;42:1-7.
doi:10.5937/jomb0-42011 .

Vicković, Sanja, Zdravković, Ranko, Maričić-Prijić, Sanja, Nikolić, Dragan, Pap, Dragana, Čolak, Emina, Jovičić, Snežana, "Salivary cortisol as a biomarker of stress in surgical patients" in Journal of Medical Biochemistry, 42 (2023):1-7,
https://doi.org/10.5937/jomb0-42011 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Vitkus, Dalius
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4402
AB  - The transformation of clinical laboratories towards digitalization requires processes that improve digital maturity. This requires establishing connectivity, end-to-end workflow, and advanced analytical technologies and techniques. Digital technologies have the key role here, directing laboratory personnel and scientists to move their focus from routine to more complex and meaningful work. This requires their empowerment in working with new instruments and software. Strategies leading clinical laboratories through this transformation are not without challenges, but different models are being developed to overcome them. The essential is the role of interoperability.
PB  - De Gruyter Open Ltd
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Digital transformation towards the clinical laboratory of the future. Perspectives for the next decade
VL  - 61
IS  - 4
SP  - 567
EP  - 569
DO  - 10.1515/cclm-2023-0001
ER  - 

@article{
author = "Jovičić, Snežana and Vitkus, Dalius",
year = "2023",
abstract = "The transformation of clinical laboratories towards digitalization requires processes that improve digital maturity. This requires establishing connectivity, end-to-end workflow, and advanced analytical technologies and techniques. Digital technologies have the key role here, directing laboratory personnel and scientists to move their focus from routine to more complex and meaningful work. This requires their empowerment in working with new instruments and software. Strategies leading clinical laboratories through this transformation are not without challenges, but different models are being developed to overcome them. The essential is the role of interoperability.",
publisher = "De Gruyter Open Ltd",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Digital transformation towards the clinical laboratory of the future. Perspectives for the next decade",
volume = "61",
number = "4",
pages = "567-569",
doi = "10.1515/cclm-2023-0001"
}

Jovičić, S.,& Vitkus, D.. (2023). Digital transformation towards the clinical laboratory of the future. Perspectives for the next decade. in Clinical Chemistry and Laboratory Medicine
De Gruyter Open Ltd., 61(4), 567-569.
https://doi.org/10.1515/cclm-2023-0001

Jovičić S, Vitkus D. Digital transformation towards the clinical laboratory of the future. Perspectives for the next decade. in Clinical Chemistry and Laboratory Medicine. 2023;61(4):567-569.
doi:10.1515/cclm-2023-0001 .

Jovičić, Snežana, Vitkus, Dalius, "Digital transformation towards the clinical laboratory of the future. Perspectives for the next decade" in Clinical Chemistry and Laboratory Medicine, 61, no. 4 (2023):567-569,
https://doi.org/10.1515/cclm-2023-0001 . .

TY  - CONF
AU  - Jovičić, Snežana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5588
AB  - Hematološki parametri su se pokazali korisnim za stratifikaciju i prognozu toka bolesti
kod pacijenata obolelih od COVID-19. Limfopenija je prepoznatljiv znak SARS-CoV-2 infekcije
i prisutna je u različitom stepenu kod skoro svih pacijenata. Postoje i indikacije da je stepen
smanjenja broja limfocita povezan sa težinom bolesti. Takođe, pokazan je i značaj niskog
broja eozinofilnih leukocita, koji u kombinaciji sa limfopenijom kod pacijenata sa simptomi-
ma predstavlja snažan indikator infekcije. Povišen broj neutrofilnih leukocita ukazuje na loš
ishod, a u kombinaciji sa niskim brojem limfocita, kada je povišen indeks neutrofilni leukociti /
limfociti, može da se koristi kao marker nepovoljne prognoze bolesti. Koagulopatija je ključna
karakteristika SARS-CoV-2 infekcije. Najčešće se manifestuje pro-trombotskim stanjem sa
povećanom incidencom venskih i arterijskih tromboza. Povišene vrednosti D-dimera su pove-
zane sa nepovoljnom progresijom bolesti. Pored toga, koagulopatija može da se manifestuje
i produženim protrombinskim i parcijalnim tromboplastinskim vremenom, kao i povišenom
koncentarcijom fibrinogena koja je posledica prisutne inflamacije. Kod osoba kod kojih dođe
do razvoja diseminovane intravaskularne koagulacije može doći do pada koncentracije fibri-
nogena i trombocitopenije. Takođe, trombocitopenija je još jedan pokazatelj nepovoljnog is-
hoda bolesti.
PB  - Udruženje farmaceuta Beograd
C3  - Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
T1  - Promene u hematološkim i parametrima hemostaze u toku infekcije SARS-CoV-2
SP  - 10
EP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5588
ER  - 

@conference{
author = "Jovičić, Snežana",
year = "2022",
abstract = "Hematološki parametri su se pokazali korisnim za stratifikaciju i prognozu toka bolesti
kod pacijenata obolelih od COVID-19. Limfopenija je prepoznatljiv znak SARS-CoV-2 infekcije
i prisutna je u različitom stepenu kod skoro svih pacijenata. Postoje i indikacije da je stepen
smanjenja broja limfocita povezan sa težinom bolesti. Takođe, pokazan je i značaj niskog
broja eozinofilnih leukocita, koji u kombinaciji sa limfopenijom kod pacijenata sa simptomi-
ma predstavlja snažan indikator infekcije. Povišen broj neutrofilnih leukocita ukazuje na loš
ishod, a u kombinaciji sa niskim brojem limfocita, kada je povišen indeks neutrofilni leukociti /
limfociti, može da se koristi kao marker nepovoljne prognoze bolesti. Koagulopatija je ključna
karakteristika SARS-CoV-2 infekcije. Najčešće se manifestuje pro-trombotskim stanjem sa
povećanom incidencom venskih i arterijskih tromboza. Povišene vrednosti D-dimera su pove-
zane sa nepovoljnom progresijom bolesti. Pored toga, koagulopatija može da se manifestuje
i produženim protrombinskim i parcijalnim tromboplastinskim vremenom, kao i povišenom
koncentarcijom fibrinogena koja je posledica prisutne inflamacije. Kod osoba kod kojih dođe
do razvoja diseminovane intravaskularne koagulacije može doći do pada koncentracije fibri-
nogena i trombocitopenije. Takođe, trombocitopenija je još jedan pokazatelj nepovoljnog is-
hoda bolesti.",
publisher = "Udruženje farmaceuta Beograd",
journal = "Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd",
title = "Promene u hematološkim i parametrima hemostaze u toku infekcije SARS-CoV-2",
pages = "10-10",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5588"
}

Jovičić, S.. (2022). Promene u hematološkim i parametrima hemostaze u toku infekcije SARS-CoV-2. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd
Udruženje farmaceuta Beograd., 10-10.
https://hdl.handle.net/21.15107/rcub_farfar_5588

Jovičić S. Promene u hematološkim i parametrima hemostaze u toku infekcije SARS-CoV-2. in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd. 2022;:10-10.
https://hdl.handle.net/21.15107/rcub_farfar_5588 .

Jovičić, Snežana, "Promene u hematološkim i parametrima hemostaze u toku infekcije SARS-CoV-2" in Simpozijum "Covid-19 infekcija-dijagnostički i prognostički biohemijski parametri", Program i zbornik sažetaka, 18.03.2022, Beograd (2022):10-10,
https://hdl.handle.net/21.15107/rcub_farfar_5588 .

TY  - JOUR
AU  - Bećarević, Mirjana
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Mirković, Duško
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4320
AB  - Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings.
AB  - Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data
T1  - Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci
VL  - 41
IS  - 4
SP  - 506
EP  - 512
DO  - 10.5937/JOMB0-35642
ER  - 

@article{
author = "Bećarević, Mirjana and Jovičić, Snežana and Ignjatović, Svetlana and Mirković, Duško",
year = "2022",
abstract = "Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings., Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data, Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci",
volume = "41",
number = "4",
pages = "506-512",
doi = "10.5937/JOMB0-35642"
}

Bećarević, M., Jovičić, S., Ignjatović, S.,& Mirković, D.. (2022). Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(4), 506-512.
https://doi.org/10.5937/JOMB0-35642

Bećarević M, Jovičić S, Ignjatović S, Mirković D. Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry. 2022;41(4):506-512.
doi:10.5937/JOMB0-35642 .

Bećarević, Mirjana, Jovičić, Snežana, Ignjatović, Svetlana, Mirković, Duško, "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data" in Journal of Medical Biochemistry, 41, no. 4 (2022):506-512,
https://doi.org/10.5937/JOMB0-35642 . .

TY  - JOUR
AU  - Vidojević, Dejana
AU  - Seman, Stefan
AU  - Lasica, Ratko
AU  - Tešić, Milorad
AU  - Sarić-Matutinović, Marija
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Arena, Ross
AU  - Damjanović, Svetozar
AU  - Popović, Dejana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3779
AB  - Purpose: Alpha-melanocyte-stimulating hormone (alpha-MSH) has proven cardiovascular effects and plays a significant role as an endogenous countermeasure to ischemia-reperfusion injury. The aim of the current study was to examine the response of alpha-MSH during exercise in patients diagnosed with coronary artery disease (CAD) and evaluate its value in the assessment of severity and prognosis. Methods: Forty subjects with documented CAD (i.e., lesions on coronary angiography ≥ 50%) were included. Cardiopulmonary exercise testing (CPET) on a treadmill (TM) and recumbent ergometer (RE) were performed on two visits, 2–4 days apart, during 2 months of coronary angiography; subsequently, the subjects were followed up for 32 ± 10 months. At rest, at peak CPET, and after 3 min of recovery, plasma levels of alpha-MSH were measured by enzyme-linked immunosorbent assay technique. Results: Mean ejection fraction was 56.7 ± 9.6%. Alpha-MSH similarly increased from rest to peak CPET on both modalities. There were no significant differences in alpha-MSH values during testing in patients with 1,2- and 3-vesel CAD, nor in patients with a SYNTAX score </≥ 23 (p > 0.05). Among CPET and hormonal parameters, ∆alpha-MSH recovery/peak during RE CPET was the best predictor of cardiac event occurrence (chi-square 6.67, HR = 0.51, CI = 0.25–1.02, p = 0.010). Conclusion: ∆alpha-MSH recovery/peak during RE CPET has predictive value for CAD prognosis, demonstrating involvement of alpha-MSH in CAD and a link between stress hormones and cardiac events.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Hormones
T1  - Alpha-melanocyte-stimulating hormone during exercise recovery has prognostic value for coronary artery disease
VL  - 20
SP  - 381
EP  - 387
DO  - 10.1007/s42000-020-00270-2
ER  - 

@article{
author = "Vidojević, Dejana and Seman, Stefan and Lasica, Ratko and Tešić, Milorad and Sarić-Matutinović, Marija and Jovičić, Snežana and Ignjatović, Svetlana and Arena, Ross and Damjanović, Svetozar and Popović, Dejana",
year = "2021",
abstract = "Purpose: Alpha-melanocyte-stimulating hormone (alpha-MSH) has proven cardiovascular effects and plays a significant role as an endogenous countermeasure to ischemia-reperfusion injury. The aim of the current study was to examine the response of alpha-MSH during exercise in patients diagnosed with coronary artery disease (CAD) and evaluate its value in the assessment of severity and prognosis. Methods: Forty subjects with documented CAD (i.e., lesions on coronary angiography ≥ 50%) were included. Cardiopulmonary exercise testing (CPET) on a treadmill (TM) and recumbent ergometer (RE) were performed on two visits, 2–4 days apart, during 2 months of coronary angiography; subsequently, the subjects were followed up for 32 ± 10 months. At rest, at peak CPET, and after 3 min of recovery, plasma levels of alpha-MSH were measured by enzyme-linked immunosorbent assay technique. Results: Mean ejection fraction was 56.7 ± 9.6%. Alpha-MSH similarly increased from rest to peak CPET on both modalities. There were no significant differences in alpha-MSH values during testing in patients with 1,2- and 3-vesel CAD, nor in patients with a SYNTAX score </≥ 23 (p > 0.05). Among CPET and hormonal parameters, ∆alpha-MSH recovery/peak during RE CPET was the best predictor of cardiac event occurrence (chi-square 6.67, HR = 0.51, CI = 0.25–1.02, p = 0.010). Conclusion: ∆alpha-MSH recovery/peak during RE CPET has predictive value for CAD prognosis, demonstrating involvement of alpha-MSH in CAD and a link between stress hormones and cardiac events.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Hormones",
title = "Alpha-melanocyte-stimulating hormone during exercise recovery has prognostic value for coronary artery disease",
volume = "20",
pages = "381-387",
doi = "10.1007/s42000-020-00270-2"
}

Vidojević, D., Seman, S., Lasica, R., Tešić, M., Sarić-Matutinović, M., Jovičić, S., Ignjatović, S., Arena, R., Damjanović, S.,& Popović, D.. (2021). Alpha-melanocyte-stimulating hormone during exercise recovery has prognostic value for coronary artery disease. in Hormones
Springer Science and Business Media Deutschland GmbH., 20, 381-387.
https://doi.org/10.1007/s42000-020-00270-2

Vidojević D, Seman S, Lasica R, Tešić M, Sarić-Matutinović M, Jovičić S, Ignjatović S, Arena R, Damjanović S, Popović D. Alpha-melanocyte-stimulating hormone during exercise recovery has prognostic value for coronary artery disease. in Hormones. 2021;20:381-387.
doi:10.1007/s42000-020-00270-2 .

Vidojević, Dejana, Seman, Stefan, Lasica, Ratko, Tešić, Milorad, Sarić-Matutinović, Marija, Jovičić, Snežana, Ignjatović, Svetlana, Arena, Ross, Damjanović, Svetozar, Popović, Dejana, "Alpha-melanocyte-stimulating hormone during exercise recovery has prognostic value for coronary artery disease" in Hormones, 20 (2021):381-387,
https://doi.org/10.1007/s42000-020-00270-2 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Siodmiak, Joanna
AU  - Alcorta, Marta Duque
AU  - Kittel, Maximillian
AU  - Oosterhuis, Wytze
AU  - Aakre, Kristin Moberg
AU  - Jørgensen, Per
AU  - Palicka, Vladimir
AU  - Kutt, Marge
AU  - Anttonen, Mikko
AU  - Georgieva Velizarova, Mileva
AU  - Marc, Jania
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3789
AB  - There are many mobile health applications (apps) now available and some that use in some way laboratory medicine data. Among them, patient-oriented are of the lowest content quality. The aim of this study was to compare the opinions of non-laboratory medicine professionals (NLMP) with those of laboratory medicine specialists (LMS) and define the benchmarks for quality assessment of laboratory medicine apps. Twenty-five volunteers from six European countries evaluated 16 selected patient-oriented apps. Participants were 20-60 years old, 44% were females, with different educational degrees, and no professional involvement in laboratory medicine. Each participant completed a questionnaire based on the Mobile Application Rating Scale (MARS) and the System Usability Scale, as previously used for rating the app quality by LMS. The responses from the two groups were compared using the Mann-Whitney U test and Spearman correlation. The median total score of NLMP app evaluation was 2.73 out of 5 (IQR 0.95) compared to 3.78 (IQR 1.05) by the LMS. All scores were statistically significantly lower in the NLMP group (p<0.05), except for the item Information quality (p=0.1631). The suggested benchmarks for a useful appear: increasing awareness of the importance and delivering an understanding of persons' own laboratory test results; understandable terminology; easy to use; appropriate graphic design, and trustworthy information. NLMP' evaluation confirmed the low utility of currently available laboratory medicine apps. A reliable app should contain trustworthy and understandable information. The appearance of an app should be fit for purpose and easy to use.
PB  - De Gruyter Open Ltd
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Quality benchmarking of smartphone laboratory medicine applications: Comparison of laboratory medicine specialists' and non-laboratory medicine professionals' evaluation
DO  - 10.1515/cclm-2020-0869
ER  - 

@article{
author = "Jovičić, Snežana and Siodmiak, Joanna and Alcorta, Marta Duque and Kittel, Maximillian and Oosterhuis, Wytze and Aakre, Kristin Moberg and Jørgensen, Per and Palicka, Vladimir and Kutt, Marge and Anttonen, Mikko and Georgieva Velizarova, Mileva and Marc, Jania",
year = "2021",
abstract = "There are many mobile health applications (apps) now available and some that use in some way laboratory medicine data. Among them, patient-oriented are of the lowest content quality. The aim of this study was to compare the opinions of non-laboratory medicine professionals (NLMP) with those of laboratory medicine specialists (LMS) and define the benchmarks for quality assessment of laboratory medicine apps. Twenty-five volunteers from six European countries evaluated 16 selected patient-oriented apps. Participants were 20-60 years old, 44% were females, with different educational degrees, and no professional involvement in laboratory medicine. Each participant completed a questionnaire based on the Mobile Application Rating Scale (MARS) and the System Usability Scale, as previously used for rating the app quality by LMS. The responses from the two groups were compared using the Mann-Whitney U test and Spearman correlation. The median total score of NLMP app evaluation was 2.73 out of 5 (IQR 0.95) compared to 3.78 (IQR 1.05) by the LMS. All scores were statistically significantly lower in the NLMP group (p<0.05), except for the item Information quality (p=0.1631). The suggested benchmarks for a useful appear: increasing awareness of the importance and delivering an understanding of persons' own laboratory test results; understandable terminology; easy to use; appropriate graphic design, and trustworthy information. NLMP' evaluation confirmed the low utility of currently available laboratory medicine apps. A reliable app should contain trustworthy and understandable information. The appearance of an app should be fit for purpose and easy to use.",
publisher = "De Gruyter Open Ltd",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Quality benchmarking of smartphone laboratory medicine applications: Comparison of laboratory medicine specialists' and non-laboratory medicine professionals' evaluation",
doi = "10.1515/cclm-2020-0869"
}

Jovičić, S., Siodmiak, J., Alcorta, M. D., Kittel, M., Oosterhuis, W., Aakre, K. M., Jørgensen, P., Palicka, V., Kutt, M., Anttonen, M., Georgieva Velizarova, M.,& Marc, J.. (2021). Quality benchmarking of smartphone laboratory medicine applications: Comparison of laboratory medicine specialists' and non-laboratory medicine professionals' evaluation. in Clinical Chemistry and Laboratory Medicine
De Gruyter Open Ltd..
https://doi.org/10.1515/cclm-2020-0869

Jovičić S, Siodmiak J, Alcorta MD, Kittel M, Oosterhuis W, Aakre KM, Jørgensen P, Palicka V, Kutt M, Anttonen M, Georgieva Velizarova M, Marc J. Quality benchmarking of smartphone laboratory medicine applications: Comparison of laboratory medicine specialists' and non-laboratory medicine professionals' evaluation. in Clinical Chemistry and Laboratory Medicine. 2021;.
doi:10.1515/cclm-2020-0869 .

Jovičić, Snežana, Siodmiak, Joanna, Alcorta, Marta Duque, Kittel, Maximillian, Oosterhuis, Wytze, Aakre, Kristin Moberg, Jørgensen, Per, Palicka, Vladimir, Kutt, Marge, Anttonen, Mikko, Georgieva Velizarova, Mileva, Marc, Jania, "Quality benchmarking of smartphone laboratory medicine applications: Comparison of laboratory medicine specialists' and non-laboratory medicine professionals' evaluation" in Clinical Chemistry and Laboratory Medicine (2021),
https://doi.org/10.1515/cclm-2020-0869 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3742
AB  - Measurement uncertainty (MU) of results is one of the basic recommended and accepted statistical
methods in laboratory medicine, with which analytical and clinical evaluation of laboratory
test quality is assessed. Literature data indicate that the calculation of MU is not a simple process,
but that its assessment in daily laboratory practice should be reduced to routine and simple
presentation, understandable to both laboratory professionals and physicians. In order to achieve
this, it is necessary to understand the purpose of the test for which MU is to be determined.
Various suggestions have been given for presentation of MU as a quantitative indicator of the
quality of the final measurement result in the medical laboratory. Although MU refers to the final
measurement result, this metrological concept reflects the entire laboratory measurement process.
The data on estimated MU is used to interpret the measured numerical result, and represents
quantitatively the quality of the measurement itself, i.e. how different are the results of
multiple measurements of the analyte of interest in the same sample, as well as whether the
method of determination itself is subjected to significant random and systematic deviation.
Initially, in the metrological concept, the MU is viewed in relation to the true value of the analyte
of interest. However, the true value of the analyte measured in the biological fluid matrix of the
study population cannot be known. It is therefore considered the closest value obtained by the
perfect method, for which the bias and inaccuracy, as measures of systematic and random error,
are equal to zero, which is practically impossible to achieve in routine laboratory practice.
Although current standards require accredited medical laboratories to estimate MU, none of
these guidelines provide clear guidance on how this can be achieved in daily laboratory work.
This review examines literary data and documents dealing with MU issues, but also highlights
what additional terms and data should be considered when interpreting MU. This paper ultimately
draws attention, and once again points out, that a simpler solution is needed for this universal
concept to be formally and universally applicable in routine laboratory medicine practice.
PB  - Taylor & Francis Group
T2  - Critical Reviews in Clinical Laboratory Sciences
T1  - Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?
DO  - 10.1080/10408363.2020.1784838
ER  - 

@article{
author = "Milinković, Neda and Jovičić, Snežana and Ignjatović, Svetlana",
year = "2020",
abstract = "Measurement uncertainty (MU) of results is one of the basic recommended and accepted statistical
methods in laboratory medicine, with which analytical and clinical evaluation of laboratory
test quality is assessed. Literature data indicate that the calculation of MU is not a simple process,
but that its assessment in daily laboratory practice should be reduced to routine and simple
presentation, understandable to both laboratory professionals and physicians. In order to achieve
this, it is necessary to understand the purpose of the test for which MU is to be determined.
Various suggestions have been given for presentation of MU as a quantitative indicator of the
quality of the final measurement result in the medical laboratory. Although MU refers to the final
measurement result, this metrological concept reflects the entire laboratory measurement process.
The data on estimated MU is used to interpret the measured numerical result, and represents
quantitatively the quality of the measurement itself, i.e. how different are the results of
multiple measurements of the analyte of interest in the same sample, as well as whether the
method of determination itself is subjected to significant random and systematic deviation.
Initially, in the metrological concept, the MU is viewed in relation to the true value of the analyte
of interest. However, the true value of the analyte measured in the biological fluid matrix of the
study population cannot be known. It is therefore considered the closest value obtained by the
perfect method, for which the bias and inaccuracy, as measures of systematic and random error,
are equal to zero, which is practically impossible to achieve in routine laboratory practice.
Although current standards require accredited medical laboratories to estimate MU, none of
these guidelines provide clear guidance on how this can be achieved in daily laboratory work.
This review examines literary data and documents dealing with MU issues, but also highlights
what additional terms and data should be considered when interpreting MU. This paper ultimately
draws attention, and once again points out, that a simpler solution is needed for this universal
concept to be formally and universally applicable in routine laboratory medicine practice.",
publisher = "Taylor & Francis Group",
journal = "Critical Reviews in Clinical Laboratory Sciences",
title = "Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?",
doi = "10.1080/10408363.2020.1784838"
}

Milinković, N., Jovičić, S.,& Ignjatović, S.. (2020). Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?. in Critical Reviews in Clinical Laboratory Sciences
Taylor & Francis Group..
https://doi.org/10.1080/10408363.2020.1784838

Milinković N, Jovičić S, Ignjatović S. Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?. in Critical Reviews in Clinical Laboratory Sciences. 2020;.
doi:10.1080/10408363.2020.1784838 .

Milinković, Neda, Jovičić, Snežana, Ignjatović, Svetlana, "Measurement uncertainty as a universal concept: can it be universally applicable in routine laboratory practice?" in Critical Reviews in Clinical Laboratory Sciences (2020),
https://doi.org/10.1080/10408363.2020.1784838 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić, Marija
AU  - Jovičić, Snežana
AU  - Mirković, Duško
AU  - Ležaić, Višnja
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3624
AB  - Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients.
AB  - Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients
T1  - Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega
VL  - 39
IS  - 3
SP  - 309
EP  - 317
DO  - 10.2478/jomb-2019-0032
ER  - 

@article{
author = "Milinković, Neda and Sarić, Marija and Jovičić, Snežana and Mirković, Duško and Ležaić, Višnja and Ignjatović, Svetlana",
year = "2020",
abstract = "Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients., Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients, Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega",
volume = "39",
number = "3",
pages = "309-317",
doi = "10.2478/jomb-2019-0032"
}

Milinković, N., Sarić, M., Jovičić, S., Mirković, D., Ležaić, V.,& Ignjatović, S.. (2020). Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 309-317.
https://doi.org/10.2478/jomb-2019-0032

Milinković N, Sarić M, Jovičić S, Mirković D, Ležaić V, Ignjatović S. Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry. 2020;39(3):309-317.
doi:10.2478/jomb-2019-0032 .

Milinković, Neda, Sarić, Marija, Jovičić, Snežana, Mirković, Duško, Ležaić, Višnja, Ignjatović, Svetlana, "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients" in Journal of Medical Biochemistry, 39, no. 3 (2020):309-317,
https://doi.org/10.2478/jomb-2019-0032 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Siodmiak, Joanna
AU  - Watson, Ian D.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3307
AB  - Background: Many of the mobile applications (apps) used for delivering health interventions involve laboratory medicine data. This survey was conducted to search the online market for health apps that manage laboratory medicine data. The aim was to review them and perform a quality evaluation. Methods: Apps search criteria were "Lab results blood work", "Lab results", and "Health apps". After the stepwise exclusion process, 52 selected apps were downloaded and analyzed. For review and content analysis of the apps, a multidimensional tool for classifying and rating the quality of mobile health apps - Mobile App Rating Scale (MARS), was used. Results: Selected apps were classified into five categories according to their intended use by patients or physicians, and the type of data engaged. Spearman's correlation analysis found significant correlations between MARS individual scoring items, as with the subjective quality and number of technical aspects. Kruskal-Wallis analysis showed a significant difference in the number of technical aspects employed, MARS engagement and informational quality score items, total score, and subjective quality. The lowest values for all of these items were in the category of apps designed for patients, and the post hoc test showed that the difference was statistically significant between this and the values in all other categories. Conclusions: Apps designed for patients, are of the poorest quality, considering the total quality of the content and information they provide, estimated using the MARS tool. This estimation needs to be validated for laboratory medicine apps, and eventually modified after consideration of specific quality benchmarks.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Quality evaluation of smartphone applications for laboratory medicine
VL  - 57
IS  - 3
SP  - 388
EP  - 397
DO  - 10.1515/cclm-2018-0710
ER  - 

@article{
author = "Jovičić, Snežana and Siodmiak, Joanna and Watson, Ian D.",
year = "2019",
abstract = "Background: Many of the mobile applications (apps) used for delivering health interventions involve laboratory medicine data. This survey was conducted to search the online market for health apps that manage laboratory medicine data. The aim was to review them and perform a quality evaluation. Methods: Apps search criteria were "Lab results blood work", "Lab results", and "Health apps". After the stepwise exclusion process, 52 selected apps were downloaded and analyzed. For review and content analysis of the apps, a multidimensional tool for classifying and rating the quality of mobile health apps - Mobile App Rating Scale (MARS), was used. Results: Selected apps were classified into five categories according to their intended use by patients or physicians, and the type of data engaged. Spearman's correlation analysis found significant correlations between MARS individual scoring items, as with the subjective quality and number of technical aspects. Kruskal-Wallis analysis showed a significant difference in the number of technical aspects employed, MARS engagement and informational quality score items, total score, and subjective quality. The lowest values for all of these items were in the category of apps designed for patients, and the post hoc test showed that the difference was statistically significant between this and the values in all other categories. Conclusions: Apps designed for patients, are of the poorest quality, considering the total quality of the content and information they provide, estimated using the MARS tool. This estimation needs to be validated for laboratory medicine apps, and eventually modified after consideration of specific quality benchmarks.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Quality evaluation of smartphone applications for laboratory medicine",
volume = "57",
number = "3",
pages = "388-397",
doi = "10.1515/cclm-2018-0710"
}

Jovičić, S., Siodmiak, J.,& Watson, I. D.. (2019). Quality evaluation of smartphone applications for laboratory medicine. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 57(3), 388-397.
https://doi.org/10.1515/cclm-2018-0710

Jovičić S, Siodmiak J, Watson ID. Quality evaluation of smartphone applications for laboratory medicine. in Clinical Chemistry and Laboratory Medicine. 2019;57(3):388-397.
doi:10.1515/cclm-2018-0710 .

Jovičić, Snežana, Siodmiak, Joanna, Watson, Ian D., "Quality evaluation of smartphone applications for laboratory medicine" in Clinical Chemistry and Laboratory Medicine, 57, no. 3 (2019):388-397,
https://doi.org/10.1515/cclm-2018-0710 . .

TY  - JOUR
AU  - Topić, Aleksandra
AU  - Francuski, Đorđe
AU  - Nikolić, Aleksandra
AU  - Milošević, Katarina
AU  - Jovičić, Snežana
AU  - Marković, Bojan
AU  - Đukić, Mirjana
AU  - Radojković, Dragica
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2833
AB  - Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Fetal and Pediatric Pathology
T1  - The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma
VL  - 36
IS  - 4
SP  - 294
EP  - 303
DO  - 10.1080/15513815.2017.1315199
ER  - 

@article{
author = "Topić, Aleksandra and Francuski, Đorđe and Nikolić, Aleksandra and Milošević, Katarina and Jovičić, Snežana and Marković, Bojan and Đukić, Mirjana and Radojković, Dragica",
year = "2017",
abstract = "Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Fetal and Pediatric Pathology",
title = "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma",
volume = "36",
number = "4",
pages = "294-303",
doi = "10.1080/15513815.2017.1315199"
}

Topić, A., Francuski, Đ., Nikolić, A., Milošević, K., Jovičić, S., Marković, B., Đukić, M.,& Radojković, D.. (2017). The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology
Taylor & Francis Inc, Philadelphia., 36(4), 294-303.
https://doi.org/10.1080/15513815.2017.1315199

Topić A, Francuski Đ, Nikolić A, Milošević K, Jovičić S, Marković B, Đukić M, Radojković D. The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology. 2017;36(4):294-303.
doi:10.1080/15513815.2017.1315199 .

Topić, Aleksandra, Francuski, Đorđe, Nikolić, Aleksandra, Milošević, Katarina, Jovičić, Snežana, Marković, Bojan, Đukić, Mirjana, Radojković, Dragica, "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma" in Fetal and Pediatric Pathology, 36, no. 4 (2017):294-303,
https://doi.org/10.1080/15513815.2017.1315199 . .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Majkić-Singh, Nada
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3018
AB  - Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate study program was established, educating Medical Biochemists under a special program. Since the academic year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and four-year postgraduate program according to EC4 European Syllabus for Postgraduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredited these programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the professional exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. In order to present laboratory medical biochemistry practice in Serbia this paper will be focused on the following: Serbian national legislation, healthcare services organization, sub-disciplines of laboratory medicine and medical biochemistry as the most significant, education in medical biochemistry, conditions for professional practice in medical biochemistry, continuous quality improvement, and accreditation. Serbian healthcare is based on fundamental principles of universal health coverage and solidarity between all citizens.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia
VL  - 36
IS  - 2
SP  - 177
EP  - 186
DO  - 10.1515/jomb-2017-0010
ER  - 

@article{
author = "Jovičić, Snežana and Majkić-Singh, Nada",
year = "2017",
abstract = "Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate study program was established, educating Medical Biochemists under a special program. Since the academic year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and four-year postgraduate program according to EC4 European Syllabus for Postgraduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredited these programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the professional exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. In order to present laboratory medical biochemistry practice in Serbia this paper will be focused on the following: Serbian national legislation, healthcare services organization, sub-disciplines of laboratory medicine and medical biochemistry as the most significant, education in medical biochemistry, conditions for professional practice in medical biochemistry, continuous quality improvement, and accreditation. Serbian healthcare is based on fundamental principles of universal health coverage and solidarity between all citizens.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia",
volume = "36",
number = "2",
pages = "177-186",
doi = "10.1515/jomb-2017-0010"
}

Jovičić, S.,& Majkić-Singh, N.. (2017). Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 36(2), 177-186.
https://doi.org/10.1515/jomb-2017-0010

Jovičić S, Majkić-Singh N. Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia. in Journal of Medical Biochemistry. 2017;36(2):177-186.
doi:10.1515/jomb-2017-0010 .

Jovičić, Snežana, Majkić-Singh, Nada, "Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia" in Journal of Medical Biochemistry, 36, no. 2 (2017):177-186,
https://doi.org/10.1515/jomb-2017-0010 . .

TY  - JOUR
AU  - Watson, Ian D.
AU  - Oosterhuis, Wytze P.
AU  - Jorgensen, Per E.
AU  - Dikmen, Zeliha Gunnur
AU  - Siodmiak, Joanna
AU  - Jovičić, Snežana
AU  - Aakre, Kristin M.
AU  - Palicka, Vladimir
AU  - Kutt, Marge
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2816
AB  - Background: There is increasing interest in direct patient engagement including receiving their laboratory medicine results. We previously established an appetite for Specialists in Laboratory Medicine to support patients in understanding results. The aim of this study was to establish whether patients agreed with such an approach, determined through surveying views in eight European countries. Methods: A standardized five-question survey was administered across eight European countries to a total of 1084 individuals attending medical outpatient clinics, with 100 patients each in Poland, Serbia, Netherlands, Turkey and Czech Republic, 101 in Estonia, 116 in Denmark and 367 in Norway. The responses across countries were compared using the chi-square test (p lt 0.05). Results: Patients wanting their results ranged from 50% to 94% (mean 65%) of those responding positively, a mean of 72% wanted additional information with their results; direct receipt was preferred over referral to a website. Specialists in Laboratory Medicine providing such information were acceptable to a mean of 62% of those respondents wishing their results; in countries where payment was possible, there was little interest in making additional payment for such a service. Conclusions: A clear proportion of patients are interested in receiving their laboratory medicine results, the majority with explanatory notes; a role for Specialists in Laboratory Medicine is acceptable and raises the potential for direct engagement by such specialists with patients offering a new paradigm for the provision of laboratory medicine activities.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - A survey of patients' views from eight European countries of interpretive support from Specialists in Laboratory Medicine
VL  - 55
IS  - 10
SP  - 1496
EP  - 1500
DO  - 10.1515/cclm-2017-0080
ER  - 

@article{
author = "Watson, Ian D. and Oosterhuis, Wytze P. and Jorgensen, Per E. and Dikmen, Zeliha Gunnur and Siodmiak, Joanna and Jovičić, Snežana and Aakre, Kristin M. and Palicka, Vladimir and Kutt, Marge",
year = "2017",
abstract = "Background: There is increasing interest in direct patient engagement including receiving their laboratory medicine results. We previously established an appetite for Specialists in Laboratory Medicine to support patients in understanding results. The aim of this study was to establish whether patients agreed with such an approach, determined through surveying views in eight European countries. Methods: A standardized five-question survey was administered across eight European countries to a total of 1084 individuals attending medical outpatient clinics, with 100 patients each in Poland, Serbia, Netherlands, Turkey and Czech Republic, 101 in Estonia, 116 in Denmark and 367 in Norway. The responses across countries were compared using the chi-square test (p lt 0.05). Results: Patients wanting their results ranged from 50% to 94% (mean 65%) of those responding positively, a mean of 72% wanted additional information with their results; direct receipt was preferred over referral to a website. Specialists in Laboratory Medicine providing such information were acceptable to a mean of 62% of those respondents wishing their results; in countries where payment was possible, there was little interest in making additional payment for such a service. Conclusions: A clear proportion of patients are interested in receiving their laboratory medicine results, the majority with explanatory notes; a role for Specialists in Laboratory Medicine is acceptable and raises the potential for direct engagement by such specialists with patients offering a new paradigm for the provision of laboratory medicine activities.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "A survey of patients' views from eight European countries of interpretive support from Specialists in Laboratory Medicine",
volume = "55",
number = "10",
pages = "1496-1500",
doi = "10.1515/cclm-2017-0080"
}

Watson, I. D., Oosterhuis, W. P., Jorgensen, P. E., Dikmen, Z. G., Siodmiak, J., Jovičić, S., Aakre, K. M., Palicka, V.,& Kutt, M.. (2017). A survey of patients' views from eight European countries of interpretive support from Specialists in Laboratory Medicine. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 55(10), 1496-1500.
https://doi.org/10.1515/cclm-2017-0080

Watson ID, Oosterhuis WP, Jorgensen PE, Dikmen ZG, Siodmiak J, Jovičić S, Aakre KM, Palicka V, Kutt M. A survey of patients' views from eight European countries of interpretive support from Specialists in Laboratory Medicine. in Clinical Chemistry and Laboratory Medicine. 2017;55(10):1496-1500.
doi:10.1515/cclm-2017-0080 .

Watson, Ian D., Oosterhuis, Wytze P., Jorgensen, Per E., Dikmen, Zeliha Gunnur, Siodmiak, Joanna, Jovičić, Snežana, Aakre, Kristin M., Palicka, Vladimir, Kutt, Marge, "A survey of patients' views from eight European countries of interpretive support from Specialists in Laboratory Medicine" in Clinical Chemistry and Laboratory Medicine, 55, no. 10 (2017):1496-1500,
https://doi.org/10.1515/cclm-2017-0080 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Bajić, Vladan
AU  - Jovičić, Snežana
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2538
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 

@article{
author = "Živković, Lada and Pirković, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan and Jovičić, Snežana and Potparević, Biljana",
year = "2016",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}

Živković, L., Pirković, A., Marčetić, M., Kovačević, N., Bajić, V., Jovičić, S.,& Potparević, B.. (2016). Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z

Živković L, Pirković A, Marčetić M, Kovačević N, Bajić V, Jovičić S, Potparević B. Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144.
doi:10.2298/ABS150512135Z .

Živković, Lada, Pirković, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan, Jovičić, Snežana, Potparević, Biljana, "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z . .

TY  - JOUR
AU  - Popević, Spasoje
AU  - Šumarac, Zorica
AU  - Jovanović, Dragana
AU  - Babić, Dragan
AU  - Stjepanović, Mihailo
AU  - Jovičić, Snežana
AU  - Sobić-Saranović, Dragana
AU  - Filipović, Snežana
AU  - Gvozdenović, Branko
AU  - Omcikus, Maja
AU  - Milovanović, Andela
AU  - Videnović-Ivanov, Jelica
AU  - Radović, Ana
AU  - Zugić, Vladimir
AU  - Vučinić-Mihailović, Violeta
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2749
AB  - Background: Until now, a proper biomarker(s) to evaluate sarcoidosis activity has not been recognized. The aims of this study were to evaluate the sensitivity and specificity of the two biomarkers of sarcoidosis activity already in use (serum angiotensin converting enzyme - ACE and serum chitotriosidase) in a population of 430 sarcoidosis patients. The activities of these markers were also analyzed in a group of 264 healthy controls. Methods: Four hundred and thirty biopsy positive sarcoidosis patients were divided into groups with active and inactive disease, and groups with acute or chronic disease. In a subgroup of 55 sarcoidosis patients, activity was also assessed by F-18 fluorodeoxyglucose positron emission tomography (F-18-FDG-PET) scanning. Both serum chitotriosidase and ACE levels showed non-normal distribution, so nonparametric tests were used in statistical analysis. Results: Serum chitotriosidase activities were almost 6 times higher in patients with active sarcoidosis than in healthy controls and inactive disease. A serum chitotriosidase value of 100 nmol/mL/h had the sensitivity of 82.5% and specificity of 70.0%. A serum ACE activity cutoff value of 32.0 U/L had the sensitivity of 66.0% and the specificity of 54%. A statistically significant correlation was obtained between the focal granulomatous activity detected on F-18-FDG PET/CT and serum chitotriosidase levels, but no such correlation was found with ACE. The levels of serum chitotriosidase activity significantly correlated with the disease duration (P lt 0.0001). Also, serum chitotriosidase significantly correlated with clinical outcome status (COS) categories (rho=0.272, P=0.001). Conclusions: Serum chitotriosidase proved to be a reliable biomarker of sarcoidosis activity and disease chronicity.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Verifying Sarcoidosis Activity: Chitotriosidase Versus ACE in Sarcoidosis - A Case-Control Study
VL  - 35
IS  - 4
SP  - 390
EP  - 400
DO  - 10.1515/jomb-2016-0017
ER  - 

@article{
author = "Popević, Spasoje and Šumarac, Zorica and Jovanović, Dragana and Babić, Dragan and Stjepanović, Mihailo and Jovičić, Snežana and Sobić-Saranović, Dragana and Filipović, Snežana and Gvozdenović, Branko and Omcikus, Maja and Milovanović, Andela and Videnović-Ivanov, Jelica and Radović, Ana and Zugić, Vladimir and Vučinić-Mihailović, Violeta",
year = "2016",
abstract = "Background: Until now, a proper biomarker(s) to evaluate sarcoidosis activity has not been recognized. The aims of this study were to evaluate the sensitivity and specificity of the two biomarkers of sarcoidosis activity already in use (serum angiotensin converting enzyme - ACE and serum chitotriosidase) in a population of 430 sarcoidosis patients. The activities of these markers were also analyzed in a group of 264 healthy controls. Methods: Four hundred and thirty biopsy positive sarcoidosis patients were divided into groups with active and inactive disease, and groups with acute or chronic disease. In a subgroup of 55 sarcoidosis patients, activity was also assessed by F-18 fluorodeoxyglucose positron emission tomography (F-18-FDG-PET) scanning. Both serum chitotriosidase and ACE levels showed non-normal distribution, so nonparametric tests were used in statistical analysis. Results: Serum chitotriosidase activities were almost 6 times higher in patients with active sarcoidosis than in healthy controls and inactive disease. A serum chitotriosidase value of 100 nmol/mL/h had the sensitivity of 82.5% and specificity of 70.0%. A serum ACE activity cutoff value of 32.0 U/L had the sensitivity of 66.0% and the specificity of 54%. A statistically significant correlation was obtained between the focal granulomatous activity detected on F-18-FDG PET/CT and serum chitotriosidase levels, but no such correlation was found with ACE. The levels of serum chitotriosidase activity significantly correlated with the disease duration (P lt 0.0001). Also, serum chitotriosidase significantly correlated with clinical outcome status (COS) categories (rho=0.272, P=0.001). Conclusions: Serum chitotriosidase proved to be a reliable biomarker of sarcoidosis activity and disease chronicity.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Verifying Sarcoidosis Activity: Chitotriosidase Versus ACE in Sarcoidosis - A Case-Control Study",
volume = "35",
number = "4",
pages = "390-400",
doi = "10.1515/jomb-2016-0017"
}

Popević, S., Šumarac, Z., Jovanović, D., Babić, D., Stjepanović, M., Jovičić, S., Sobić-Saranović, D., Filipović, S., Gvozdenović, B., Omcikus, M., Milovanović, A., Videnović-Ivanov, J., Radović, A., Zugić, V.,& Vučinić-Mihailović, V.. (2016). Verifying Sarcoidosis Activity: Chitotriosidase Versus ACE in Sarcoidosis - A Case-Control Study. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(4), 390-400.
https://doi.org/10.1515/jomb-2016-0017

Popević S, Šumarac Z, Jovanović D, Babić D, Stjepanović M, Jovičić S, Sobić-Saranović D, Filipović S, Gvozdenović B, Omcikus M, Milovanović A, Videnović-Ivanov J, Radović A, Zugić V, Vučinić-Mihailović V. Verifying Sarcoidosis Activity: Chitotriosidase Versus ACE in Sarcoidosis - A Case-Control Study. in Journal of Medical Biochemistry. 2016;35(4):390-400.
doi:10.1515/jomb-2016-0017 .

Popević, Spasoje, Šumarac, Zorica, Jovanović, Dragana, Babić, Dragan, Stjepanović, Mihailo, Jovičić, Snežana, Sobić-Saranović, Dragana, Filipović, Snežana, Gvozdenović, Branko, Omcikus, Maja, Milovanović, Andela, Videnović-Ivanov, Jelica, Radović, Ana, Zugić, Vladimir, Vučinić-Mihailović, Violeta, "Verifying Sarcoidosis Activity: Chitotriosidase Versus ACE in Sarcoidosis - A Case-Control Study" in Journal of Medical Biochemistry, 35, no. 4 (2016):390-400,
https://doi.org/10.1515/jomb-2016-0017 . .

TY  - JOUR
AU  - Kangrga, Ranka
AU  - Ignjatović, Svetlana
AU  - Dragasević, Mirjana M.
AU  - Jovičić, Snežana
AU  - Majkić-Singh, Nada
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2546
AB  - Objective: The measurement of pancreatic elastase (PE) in feces is used widely to screen for pancreatic exocrine insufficiency. The aim of our study was to evaluate the relationship of PE with residual beta cell secretion and metabolic control in patients with diabetes mellitus. Method: We determined the presence of PE in specimens via enzyme-linked immunosorbent assay (ELISA), whereas serum fasting glucose, C-peptide, amylase, lipase, triglycerides, total 25(OH)-vitamin D, C-reactive protein (CRP), and hemoglobin A(1c) (HbA(1c)) concentrations were assayed using routine laboratory tests. Results: PE values in 48 patients with diabetes were significantly lower than in 24 healthy volunteers (P = .001). In one-third of participants with diabetes mellitus, PE were less than 200 mu g per g, indicating pancreatic functional insufficiency. Among the patients in the cohort, PE correlated positively with C-peptide levels (P = .04), lipase (P = .009), CRP (P = .04), sex (P = .03), and BMI (P = .02) but not significantly with duration of diabetes (P = .81) or levels of HbA(1c) (P = .87), amylase (P = .06), total 25(OH)-vitamin D (P = .16), or triglycerides (P = .52). Conclusion: Our results demonstrated a strong association of diabetes with low PE levels.
PB  - Oxford Univ Press, Oxford
T2  - Labmedicine
T1  - Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus
VL  - 47
IS  - 2
SP  - 140
EP  - 148
DO  - 10.1093/labmed/lmw015
ER  - 

@article{
author = "Kangrga, Ranka and Ignjatović, Svetlana and Dragasević, Mirjana M. and Jovičić, Snežana and Majkić-Singh, Nada",
year = "2016",
abstract = "Objective: The measurement of pancreatic elastase (PE) in feces is used widely to screen for pancreatic exocrine insufficiency. The aim of our study was to evaluate the relationship of PE with residual beta cell secretion and metabolic control in patients with diabetes mellitus. Method: We determined the presence of PE in specimens via enzyme-linked immunosorbent assay (ELISA), whereas serum fasting glucose, C-peptide, amylase, lipase, triglycerides, total 25(OH)-vitamin D, C-reactive protein (CRP), and hemoglobin A(1c) (HbA(1c)) concentrations were assayed using routine laboratory tests. Results: PE values in 48 patients with diabetes were significantly lower than in 24 healthy volunteers (P = .001). In one-third of participants with diabetes mellitus, PE were less than 200 mu g per g, indicating pancreatic functional insufficiency. Among the patients in the cohort, PE correlated positively with C-peptide levels (P = .04), lipase (P = .009), CRP (P = .04), sex (P = .03), and BMI (P = .02) but not significantly with duration of diabetes (P = .81) or levels of HbA(1c) (P = .87), amylase (P = .06), total 25(OH)-vitamin D (P = .16), or triglycerides (P = .52). Conclusion: Our results demonstrated a strong association of diabetes with low PE levels.",
publisher = "Oxford Univ Press, Oxford",
journal = "Labmedicine",
title = "Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus",
volume = "47",
number = "2",
pages = "140-148",
doi = "10.1093/labmed/lmw015"
}

Kangrga, R., Ignjatović, S., Dragasević, M. M., Jovičić, S.,& Majkić-Singh, N.. (2016). Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus. in Labmedicine
Oxford Univ Press, Oxford., 47(2), 140-148.
https://doi.org/10.1093/labmed/lmw015

Kangrga R, Ignjatović S, Dragasević MM, Jovičić S, Majkić-Singh N. Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus. in Labmedicine. 2016;47(2):140-148.
doi:10.1093/labmed/lmw015 .

Kangrga, Ranka, Ignjatović, Svetlana, Dragasević, Mirjana M., Jovičić, Snežana, Majkić-Singh, Nada, "Pancreatic Elastase Levels in Feces As A Marker of Exocrine Pancreatic Function in Patients With Diabetes Mellitus" in Labmedicine, 47, no. 2 (2016):140-148,
https://doi.org/10.1093/labmed/lmw015 . .

TY  - CONF
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Dajak, Marijana
AU  - Majkić-Singh, Nada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5491
AB  - U kliničkoj praksi koristi se nekoliko skorova za
procenu kratkoročnog (10-godišnjeg) rizika od
pojave različitih oblika kardiovaskularnih bolesti
(KVB) koji se zasnivaju na multivarijabilnim regre-
sionim jednačinama izvedenim iz rezultata praćenja
različitih kohortnih grupa. Međutim, pošto je starost
promenljiva kojoj se dodeljuje najveći broj poena u
modelima 10-godišnjeg rizika, mnoge osobe sa zna
čajnim opterećenjem faktorima rizika imaju kratko-
ročni rizik daleko ispod granice koja uslovljava inten-
zivan tretman, iako njihov dugoročni (30-godišnji)
rizik može biti značajan. Takođe, drugi biomarkeri
mogu da identifikuju osobe sa većim kardiovasku-
larnim rizikom od onog izračunatog primenom skoro-
va kratkoročnog rizika. Cilj rada bio je da se analizira
priroda uticaja ispitivanih biomarkera na kardiovas-
kularni rizik i njihovo grupisanje, kao i povezanost
dobijenih faktora sa kategorizacijom 30-godišnjeg
rizika faktorskom analizom. Pomoću interaktivnog
kalkulatora »30-year risk of cardiovascular disease«
izračunavan je dugoročni 30-godišnji rizik za pojavu
»kompletne« KVB (sve manifestacije KVB) i »teške«
KVB (potencijalno fatalne komplikacije KVB). Analiza
glavnih komponenti je korišćena za ispitivanje
grupisanja markera inflamacije [visoko-osetljivi C-
reaktivni protein (hsCRP), serumski amiloid A (SAA),
fibrinogen, a1-kiseli glikoprotein (A1AGP), haptoglo-
bin, C3 i C4 komponente komplementa], metabo-lizma lipida [non-HDL i LDL holesterol, trigliceridi,
apolipoprotein A-I (apo A-I), apolipoprotein B (apo
B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin,
mokraćna kiselina, cistatin C (Cys-C)] i srčane funk-
cije [N-terminalni pro-natriuretički peptid tip B (NT-
proBNP), visoko-osetljivi srčani troponin T (hs-cTnT)],
dobijenih analizom uzoraka seruma 242 zdrave oso-
be. Faktorskom analizom identifikovano je 5 klastera,
kojima je objašnjeno je 67,4% ukupne varijacije, ras-
poređene na sledeći način 1) 29,7% »sistemska infla-
macija« (hsCRP, fibrinogen, SAA, A1AGP, haptoglo-
bin, C3 i C4 komponenta komplementa); 2) 12,5%
»aterogena dislipidemija« (LDL i non-HDL holesterol,
apo B i trigliceridi); 3) 11,0% »kardiorenalni faktor«
(kreatinin, mokraćna kiselina, Cys-C i hs-cTnT); 4)
7,6% »hemodinamski faktor« (NT-proBNP) i 5) 6,7%
»lipoproteinski faktor« [apo A-I, Lp(a)]. Prediktivne
vrednosti u proceni 30-godišnjeg rizika za »komplet-
nu KVB« i »tešku KVB« su bile značajne za četiri fak-
tora (OR 1,892–5,590; P<0,0001 i OR 2,183–
5,931; P<0,0001, redom), a »hemodinamski fak-
tor« nije imao statistički značajan prediktivni potenci-
jal za vrednosti iznad optimalnih/normalnih za odgo-
varajući pol i starost (P>0,05). Površine ispod ROC
krivih (AUC) modela sa pet faktora u predikciji
povećanog 30-godišnjeg rizika za »kompletnu KVB« i
»tešku KVB« iznosile su 0,881 i 0,888, redom, i nisu
bile statistički značajno različite od multivarijabilnog
logističkog modela od 18 polaznih parametara
(0,892 i 0,901; P>0,05; redom). Sistemska inflama-
cija, aterogena dislipidemija, kardiorenalna funkcija i
lipoproteinski status nezavisno doprinose dugo-
ročnom, 30-godišnjem riziku iznad normalnog/opti-
malnog kako za ozbiljne komplikacije KVB, tako i za
sve vrste kardiovaskularnih komplikacija.
AB  - Several risk score algorithms for short-term (10-
year) cardiovascular risk assessment based on multi-
variable regression equations derived from different
cohorts are being used in clinical practice. However,
since the age is variable with the strongest influence
on short-term risk, many individuals with moderate
increase of other traditional risk factors would have a
10-year risk below cutoff for intensive treatment, but
a significant long-term (30-year) risk. Also, other bio-
markers might identify persons with higher actual
cardiovascular risk compared with calculated using
short-term risk scores. The aim of this study was to
analyze the nature of influence of examined biomark-
ers on cardiovascular risk and their clustering, as well
as relations of identified factors with long-term 30-
year risk categorization, using factor analysis.
Interactive calculator »30-year risk of cardiovascular
disease« was used for long-term 30-year risk calcula-
tion, for both »full CVD« (all manifestations of cardio-
vascular disease) and »hard CVD« (serious manifesta-
tions of CVD). Principal component analysis was used
to investigate clustering of markers of inflammation
[high sensitivity C-reactive protein (hsCRP), serum
amyloid A (SAA), fibrinogen, a1-acid glycoprotein
(A1AGP), haptoglobin, C3 and C4 complement
components], lipid metabolism [non-HDL and LDL
cholesterol, triglycerides, apolipoprotein A-I (apo A-
I), apolipoprotein B (apo B), lipoprotein (a) (Lp(a))], renal [creatinine, uric acid, cystatin C (Cys-C)] and
cardiac function [N-terminal pro-natriuretic peptide
type B (NT-proBNP), high sensitivity cardiac troponin
T (hs-cTnT)], obtained from 242 apparently healthy
individuals. Factor analysis identified five clusters,
which explained 67.4% of the total variance distrib-
uted as follows: 1) 29.7% »systemic inflammation«
(hsCRP, fibrinogen, SAA, A1AGP, haptoglobin, C3,
C4); 2) 12.5% »atherogenic dyslipidemia«, (LDL and
non-HDL cholesterol, apo B, triglycerides); 3) 11.0%
»cardiorenal factor« (creatinine, uric acid, Cys-C, hs-
cTnT); 4) 7.6% »hemodynamic factor« (NT-proBNP);
and 5) 6.7% »lipoprotein factor« [apo A-I, Lp(a)].
When estimating 30-year risk from both »full CVD«
and »hard CVD«, predictive values were significant
for four factors (OR 1.892–5.590, P<0.0001 and
OR 2.183–5.931, P<0.0001, respectively), and
»hemodynamic factor« had no statistical significance
in predicting potential for values above optimal/nor-
mal for corresponding gender and age (P>0.05).
The areas under the receiver operating characteristic
curves (AUCs) of the five factor model in predicting
increased 30-year risk for »full CVD« and »hard CVD«
were 0.881 and 0.888, respectively, which were not
statistically significantly different from AUCs of the
multivariable logistic model of 18 original parameters
(0.892 and 0.901, P>0.05, respectively). Long-
term, 30-year risk above normal/optimal for hard
CVD complications and for all kinds of cardiovascular
complications was independently contributed by sys-
temic inflammation, atherogenic dyslipidemia, car-
diorenal function and lipoprotein status.
PB  - Društvo medicinskih biohemičara Srbije, Beograd
C3  - XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.
T1  - Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika
T1  - Factor analysis of association of lipid, inflammatory, cardiac and renal biomarkers with long-term 30-year cardiovascular risk classification
VL  - 34
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5491
ER  - 

@conference{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Dajak, Marijana and Majkić-Singh, Nada",
year = "2015",
abstract = "U kliničkoj praksi koristi se nekoliko skorova za
procenu kratkoročnog (10-godišnjeg) rizika od
pojave različitih oblika kardiovaskularnih bolesti
(KVB) koji se zasnivaju na multivarijabilnim regre-
sionim jednačinama izvedenim iz rezultata praćenja
različitih kohortnih grupa. Međutim, pošto je starost
promenljiva kojoj se dodeljuje najveći broj poena u
modelima 10-godišnjeg rizika, mnoge osobe sa zna
čajnim opterećenjem faktorima rizika imaju kratko-
ročni rizik daleko ispod granice koja uslovljava inten-
zivan tretman, iako njihov dugoročni (30-godišnji)
rizik može biti značajan. Takođe, drugi biomarkeri
mogu da identifikuju osobe sa većim kardiovasku-
larnim rizikom od onog izračunatog primenom skoro-
va kratkoročnog rizika. Cilj rada bio je da se analizira
priroda uticaja ispitivanih biomarkera na kardiovas-
kularni rizik i njihovo grupisanje, kao i povezanost
dobijenih faktora sa kategorizacijom 30-godišnjeg
rizika faktorskom analizom. Pomoću interaktivnog
kalkulatora »30-year risk of cardiovascular disease«
izračunavan je dugoročni 30-godišnji rizik za pojavu
»kompletne« KVB (sve manifestacije KVB) i »teške«
KVB (potencijalno fatalne komplikacije KVB). Analiza
glavnih komponenti je korišćena za ispitivanje
grupisanja markera inflamacije [visoko-osetljivi C-
reaktivni protein (hsCRP), serumski amiloid A (SAA),
fibrinogen, a1-kiseli glikoprotein (A1AGP), haptoglo-
bin, C3 i C4 komponente komplementa], metabo-lizma lipida [non-HDL i LDL holesterol, trigliceridi,
apolipoprotein A-I (apo A-I), apolipoprotein B (apo
B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin,
mokraćna kiselina, cistatin C (Cys-C)] i srčane funk-
cije [N-terminalni pro-natriuretički peptid tip B (NT-
proBNP), visoko-osetljivi srčani troponin T (hs-cTnT)],
dobijenih analizom uzoraka seruma 242 zdrave oso-
be. Faktorskom analizom identifikovano je 5 klastera,
kojima je objašnjeno je 67,4% ukupne varijacije, ras-
poređene na sledeći način 1) 29,7% »sistemska infla-
macija« (hsCRP, fibrinogen, SAA, A1AGP, haptoglo-
bin, C3 i C4 komponenta komplementa); 2) 12,5%
»aterogena dislipidemija« (LDL i non-HDL holesterol,
apo B i trigliceridi); 3) 11,0% »kardiorenalni faktor«
(kreatinin, mokraćna kiselina, Cys-C i hs-cTnT); 4)
7,6% »hemodinamski faktor« (NT-proBNP) i 5) 6,7%
»lipoproteinski faktor« [apo A-I, Lp(a)]. Prediktivne
vrednosti u proceni 30-godišnjeg rizika za »komplet-
nu KVB« i »tešku KVB« su bile značajne za četiri fak-
tora (OR 1,892–5,590; P<0,0001 i OR 2,183–
5,931; P<0,0001, redom), a »hemodinamski fak-
tor« nije imao statistički značajan prediktivni potenci-
jal za vrednosti iznad optimalnih/normalnih za odgo-
varajući pol i starost (P>0,05). Površine ispod ROC
krivih (AUC) modela sa pet faktora u predikciji
povećanog 30-godišnjeg rizika za »kompletnu KVB« i
»tešku KVB« iznosile su 0,881 i 0,888, redom, i nisu
bile statistički značajno različite od multivarijabilnog
logističkog modela od 18 polaznih parametara
(0,892 i 0,901; P>0,05; redom). Sistemska inflama-
cija, aterogena dislipidemija, kardiorenalna funkcija i
lipoproteinski status nezavisno doprinose dugo-
ročnom, 30-godišnjem riziku iznad normalnog/opti-
malnog kako za ozbiljne komplikacije KVB, tako i za
sve vrste kardiovaskularnih komplikacija., Several risk score algorithms for short-term (10-
year) cardiovascular risk assessment based on multi-
variable regression equations derived from different
cohorts are being used in clinical practice. However,
since the age is variable with the strongest influence
on short-term risk, many individuals with moderate
increase of other traditional risk factors would have a
10-year risk below cutoff for intensive treatment, but
a significant long-term (30-year) risk. Also, other bio-
markers might identify persons with higher actual
cardiovascular risk compared with calculated using
short-term risk scores. The aim of this study was to
analyze the nature of influence of examined biomark-
ers on cardiovascular risk and their clustering, as well
as relations of identified factors with long-term 30-
year risk categorization, using factor analysis.
Interactive calculator »30-year risk of cardiovascular
disease« was used for long-term 30-year risk calcula-
tion, for both »full CVD« (all manifestations of cardio-
vascular disease) and »hard CVD« (serious manifesta-
tions of CVD). Principal component analysis was used
to investigate clustering of markers of inflammation
[high sensitivity C-reactive protein (hsCRP), serum
amyloid A (SAA), fibrinogen, a1-acid glycoprotein
(A1AGP), haptoglobin, C3 and C4 complement
components], lipid metabolism [non-HDL and LDL
cholesterol, triglycerides, apolipoprotein A-I (apo A-
I), apolipoprotein B (apo B), lipoprotein (a) (Lp(a))], renal [creatinine, uric acid, cystatin C (Cys-C)] and
cardiac function [N-terminal pro-natriuretic peptide
type B (NT-proBNP), high sensitivity cardiac troponin
T (hs-cTnT)], obtained from 242 apparently healthy
individuals. Factor analysis identified five clusters,
which explained 67.4% of the total variance distrib-
uted as follows: 1) 29.7% »systemic inflammation«
(hsCRP, fibrinogen, SAA, A1AGP, haptoglobin, C3,
C4); 2) 12.5% »atherogenic dyslipidemia«, (LDL and
non-HDL cholesterol, apo B, triglycerides); 3) 11.0%
»cardiorenal factor« (creatinine, uric acid, Cys-C, hs-
cTnT); 4) 7.6% »hemodynamic factor« (NT-proBNP);
and 5) 6.7% »lipoprotein factor« [apo A-I, Lp(a)].
When estimating 30-year risk from both »full CVD«
and »hard CVD«, predictive values were significant
for four factors (OR 1.892–5.590, P<0.0001 and
OR 2.183–5.931, P<0.0001, respectively), and
»hemodynamic factor« had no statistical significance
in predicting potential for values above optimal/nor-
mal for corresponding gender and age (P>0.05).
The areas under the receiver operating characteristic
curves (AUCs) of the five factor model in predicting
increased 30-year risk for »full CVD« and »hard CVD«
were 0.881 and 0.888, respectively, which were not
statistically significantly different from AUCs of the
multivariable logistic model of 18 original parameters
(0.892 and 0.901, P>0.05, respectively). Long-
term, 30-year risk above normal/optimal for hard
CVD complications and for all kinds of cardiovascular
complications was independently contributed by sys-
temic inflammation, atherogenic dyslipidemia, car-
diorenal function and lipoprotein status.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd",
journal = "XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.",
title = "Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika, Factor analysis of association of lipid, inflammatory, cardiac and renal biomarkers with long-term 30-year cardiovascular risk classification",
volume = "34",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5491"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Dajak, M.,& Majkić-Singh, N.. (2015). Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika. in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.
Društvo medicinskih biohemičara Srbije, Beograd., 34, 68-69.
https://hdl.handle.net/21.15107/rcub_farfar_5491

Jovičić S, Ignjatović S, Kangrga R, Dajak M, Majkić-Singh N. Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika. in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015.. 2015;34:68-69.
https://hdl.handle.net/21.15107/rcub_farfar_5491 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Dajak, Marijana, Majkić-Singh, Nada, "Faktorska analiza povezanosti inflamatornih, lipidnih, srčanih i bubrežnih biomarkera sa Klasifikacijom dugoročnog 30-godišnjeg kardiovaskularnog rizika" in XIX kongres medicinske i laboratorijske medicine sa me|unarodnim učešćem, 2015, Journal of Medical Chemistry, 34, 1, 2015., 34 (2015):68-69,
https://hdl.handle.net/21.15107/rcub_farfar_5491 .

TY  - JOUR
AU  - Watson, Ian D.
AU  - Siodmiak, Joanna
AU  - Oosterhuis, Wytze P.
AU  - Corberand, Joel
AU  - Jorgensen, Per E.
AU  - Dikmen, Zeliha Gunnur
AU  - Jovičić, Snežana
AU  - Theodorsson, Elvar
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2302
AB  - Background: Medicine is a highly professionalized endeavour, by tradition centred on the authority of physicians. Better education and the advent of the information age cater for increased demands on society in general and on health care in particular to enable people to make informed decisions regarding themselves. Participation in medical decisions requires informed knowledge which is hard to obtain without substantial and time consuming professional help. Methods: We performed a survey amongst the member organizations of European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) in order to investigate the recognition and preparedness of providing help to patients in interpreting their laboratory results. Results: Out of 40 EFLM Member Societies, 27 sent their responses to the survey. In most cases the first line delivery of laboratory results to physicians is by computer link (63%). Patients receive their laboratory results on demand from their physician in 60% of cases. However, 34% of laboratory specialists showed a negative attitude for delivering laboratory results to patients. Yet, in 48% of countries 1-5 patients per day ask a laboratory specialist about the significance of laboratory results outside the reference range. When patients are informed about the purpose of laboratory testing, they seek information primarily from their physician, followed by the internet and the Specialist in Laboratory Medicine. Conclusions: Changing practices increasingly enabling patient access to their records are on the increase facilitated by recent innovations in information technologies. Successful transfer of some of the responsibilities of physicians, demands a mutual triangular dialogue between the patient, their physician and laboratory medicine.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - European views on patients directly obtaining their laboratory test results
VL  - 53
IS  - 12
SP  - 1961
EP  - 1966
DO  - 10.1515/cclm-2015-0056
ER  - 

@article{
author = "Watson, Ian D. and Siodmiak, Joanna and Oosterhuis, Wytze P. and Corberand, Joel and Jorgensen, Per E. and Dikmen, Zeliha Gunnur and Jovičić, Snežana and Theodorsson, Elvar",
year = "2015",
abstract = "Background: Medicine is a highly professionalized endeavour, by tradition centred on the authority of physicians. Better education and the advent of the information age cater for increased demands on society in general and on health care in particular to enable people to make informed decisions regarding themselves. Participation in medical decisions requires informed knowledge which is hard to obtain without substantial and time consuming professional help. Methods: We performed a survey amongst the member organizations of European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) in order to investigate the recognition and preparedness of providing help to patients in interpreting their laboratory results. Results: Out of 40 EFLM Member Societies, 27 sent their responses to the survey. In most cases the first line delivery of laboratory results to physicians is by computer link (63%). Patients receive their laboratory results on demand from their physician in 60% of cases. However, 34% of laboratory specialists showed a negative attitude for delivering laboratory results to patients. Yet, in 48% of countries 1-5 patients per day ask a laboratory specialist about the significance of laboratory results outside the reference range. When patients are informed about the purpose of laboratory testing, they seek information primarily from their physician, followed by the internet and the Specialist in Laboratory Medicine. Conclusions: Changing practices increasingly enabling patient access to their records are on the increase facilitated by recent innovations in information technologies. Successful transfer of some of the responsibilities of physicians, demands a mutual triangular dialogue between the patient, their physician and laboratory medicine.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "European views on patients directly obtaining their laboratory test results",
volume = "53",
number = "12",
pages = "1961-1966",
doi = "10.1515/cclm-2015-0056"
}

Watson, I. D., Siodmiak, J., Oosterhuis, W. P., Corberand, J., Jorgensen, P. E., Dikmen, Z. G., Jovičić, S.,& Theodorsson, E.. (2015). European views on patients directly obtaining their laboratory test results. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 53(12), 1961-1966.
https://doi.org/10.1515/cclm-2015-0056

Watson ID, Siodmiak J, Oosterhuis WP, Corberand J, Jorgensen PE, Dikmen ZG, Jovičić S, Theodorsson E. European views on patients directly obtaining their laboratory test results. in Clinical Chemistry and Laboratory Medicine. 2015;53(12):1961-1966.
doi:10.1515/cclm-2015-0056 .

Watson, Ian D., Siodmiak, Joanna, Oosterhuis, Wytze P., Corberand, Joel, Jorgensen, Per E., Dikmen, Zeliha Gunnur, Jovičić, Snežana, Theodorsson, Elvar, "European views on patients directly obtaining their laboratory test results" in Clinical Chemistry and Laboratory Medicine, 53, no. 12 (2015):1961-1966,
https://doi.org/10.1515/cclm-2015-0056 . .

TY  - JOUR
AU  - Vucelić, Dragica
AU  - Jesić, Rada
AU  - Jovičić, Snežana
AU  - Zivotić, Maja
AU  - Grubor, Nikica
AU  - Trajković, Goran
AU  - Canić, Ivana
AU  - Elezović, Ivo
AU  - Antović, Aleksandra
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2396
AB  - Background: The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing information on both quantitative deficit and fibrin polymerization disorders. Objective: To compare these three methods of assessing fibrinogen in patients with cirrhosis of different aetiologies, characterized by impairment in fibrinogen concentration as well as functional aberrance. Methods: Sixty patients with alcoholic and 24 patients with cholestatic cirrhosis were included (Child-Pugh score (CPs) A, n = 24; B, n = 32; C, n = 28). All parameters were compared with those from a control group. Maximum clot firmness (MCF) in the FIBTEM test was assessed in regard to its relevance in detection of qualitative fibrinogen disorders in comparison with results obtained by standard measurement methods, i.e. the Clauss fibrinogen method and TCT. Results: With increased cirrhosis severity, fibrinogen and FIBTEM-MCF levels significantly declined (p = 0.002), while TCT was significantly prolonged (p = 0.002). In all CPs groups, fibrinogen strongly correlated with FIBTEM-MCF (r = 0.77, r = 0.72, r = 0.74; p  lt  0.001), while cross-correlations of other assays were highly variable. The prevalence of decreased FIBTEM-MCF values ( lt 9 mm) was significantly higher in advanced CPs categories (p = 0.027), whereby the highest prevalence was detected in patients with CPsC (10/16; 62.5%). Nine of the 16 patients with decreased FIBTEM-MCF values had also decreased fibrinogen levels, while in the remaining 7 patients fibrinogen levels were within the reference range, indicating the possible presence of qualitatively altered fibrinogen that could be detected by FIBTEM-MCF. Conclusions: FIBTEM-MCF may be considered as a reliable alternative to standard plasma fibrinogen measurement in cirrhotic patients, especially in evaluating fibrin polymerization disorders in these patients. Further studies are needed to evaluate the usefulness of this assay in predicting bleeding complications in cirrhotic patients as well as monitoring replacement treatment.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Tohoku Journal of Experimental Medicine
T1  - Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis
VL  - 135
IS  - 6
SP  - 1124
EP  - 1130
DO  - 10.1016/j.thromres.2015.04.003
ER  - 

@article{
author = "Vucelić, Dragica and Jesić, Rada and Jovičić, Snežana and Zivotić, Maja and Grubor, Nikica and Trajković, Goran and Canić, Ivana and Elezović, Ivo and Antović, Aleksandra",
year = "2015",
abstract = "Background: The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing information on both quantitative deficit and fibrin polymerization disorders. Objective: To compare these three methods of assessing fibrinogen in patients with cirrhosis of different aetiologies, characterized by impairment in fibrinogen concentration as well as functional aberrance. Methods: Sixty patients with alcoholic and 24 patients with cholestatic cirrhosis were included (Child-Pugh score (CPs) A, n = 24; B, n = 32; C, n = 28). All parameters were compared with those from a control group. Maximum clot firmness (MCF) in the FIBTEM test was assessed in regard to its relevance in detection of qualitative fibrinogen disorders in comparison with results obtained by standard measurement methods, i.e. the Clauss fibrinogen method and TCT. Results: With increased cirrhosis severity, fibrinogen and FIBTEM-MCF levels significantly declined (p = 0.002), while TCT was significantly prolonged (p = 0.002). In all CPs groups, fibrinogen strongly correlated with FIBTEM-MCF (r = 0.77, r = 0.72, r = 0.74; p  lt  0.001), while cross-correlations of other assays were highly variable. The prevalence of decreased FIBTEM-MCF values ( lt 9 mm) was significantly higher in advanced CPs categories (p = 0.027), whereby the highest prevalence was detected in patients with CPsC (10/16; 62.5%). Nine of the 16 patients with decreased FIBTEM-MCF values had also decreased fibrinogen levels, while in the remaining 7 patients fibrinogen levels were within the reference range, indicating the possible presence of qualitatively altered fibrinogen that could be detected by FIBTEM-MCF. Conclusions: FIBTEM-MCF may be considered as a reliable alternative to standard plasma fibrinogen measurement in cirrhotic patients, especially in evaluating fibrin polymerization disorders in these patients. Further studies are needed to evaluate the usefulness of this assay in predicting bleeding complications in cirrhotic patients as well as monitoring replacement treatment.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Tohoku Journal of Experimental Medicine",
title = "Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis",
volume = "135",
number = "6",
pages = "1124-1130",
doi = "10.1016/j.thromres.2015.04.003"
}

Vucelić, D., Jesić, R., Jovičić, S., Zivotić, M., Grubor, N., Trajković, G., Canić, I., Elezović, I.,& Antović, A.. (2015). Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis. in Tohoku Journal of Experimental Medicine
Pergamon-Elsevier Science Ltd, Oxford., 135(6), 1124-1130.
https://doi.org/10.1016/j.thromres.2015.04.003

Vucelić D, Jesić R, Jovičić S, Zivotić M, Grubor N, Trajković G, Canić I, Elezović I, Antović A. Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis. in Tohoku Journal of Experimental Medicine. 2015;135(6):1124-1130.
doi:10.1016/j.thromres.2015.04.003 .

Vucelić, Dragica, Jesić, Rada, Jovičić, Snežana, Zivotić, Maja, Grubor, Nikica, Trajković, Goran, Canić, Ivana, Elezović, Ivo, Antović, Aleksandra, "Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis" in Tohoku Journal of Experimental Medicine, 135, no. 6 (2015):1124-1130,
https://doi.org/10.1016/j.thromres.2015.04.003 . .

TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Jovičić, Snežana
AU  - Radosavljević, Branimir
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2099
AB  - Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results
VL  - 74
IS  - 5
SP  - 378
EP  - 384
DO  - 10.3109/00365513.2014.898324
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Jovičić, Snežana and Radosavljević, Branimir and Singh, Sandra and Majkić-Singh, Nada",
year = "2014",
abstract = "Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results",
volume = "74",
number = "5",
pages = "378-384",
doi = "10.3109/00365513.2014.898324"
}

Milinković, N., Ignjatović, S., Žarković, M., Jovičić, S., Radosavljević, B., Singh, S.,& Majkić-Singh, N.. (2014). Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 74(5), 378-384.
https://doi.org/10.3109/00365513.2014.898324

Milinković N, Ignjatović S, Žarković M, Jovičić S, Radosavljević B, Singh S, Majkić-Singh N. Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation. 2014;74(5):378-384.
doi:10.3109/00365513.2014.898324 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Jovičić, Snežana, Radosavljević, Branimir, Singh, Sandra, Majkić-Singh, Nada, "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results" in Scandinavian Journal of Clinical and Laboratory Investigation, 74, no. 5 (2014):378-384,
https://doi.org/10.3109/00365513.2014.898324 . .

TY  - JOUR
AU  - Seferović, Jelena
AU  - Lalić, Nebojša M.
AU  - Floridi, Federico
AU  - Tesić, Milorad
AU  - Seferović, Petar M.
AU  - Giga, Vojislav
AU  - Lalić, Katarina
AU  - Jotić, Aleksandra
AU  - Jovičić, Snežana
AU  - Colak, Emina
AU  - Salerno, Gerardo
AU  - Cardelli, Patrizia
AU  - Di Somma, Salvatore
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2074
AB  - Background: Galectin-3 is a protein widely distributed in the heart, brain and blood vessels, and has a regulatory role in inflammation, immunology and cancer. Many studies demonstrated that the increased level of galectin-3 is associated with progressive fibrosis and stiffening of the myocardium. The aim of this study was to investigate the role of galectin-3 in patients with type 2 diabetes (T2D) and/or arterial hypertension (HT). Methods: Study population included 189 patients, with no coronary artery disease, divided into three groups: group 1 (T2D), group 2 (T2D+HT), and group 3 (HT). All subjects underwent routine laboratory tests, as well as specific biomarkers assessment [galectin-3, glycosylated hemoglobin (HbA(1c)), N- terminal fragment B-type natriuretic peptide (NT-proBNP)]. Cardiological evaluation included physical examination, transthoracic tissue Doppler echocardiography and stress echocardiography. Results: The results of this study demonstrated significantly increased levels of galectin-3, blood glucose, and HbA(1c) in group 2. Also, echocardiographicaly, left ventricular (LV) diameters and IVS thickness were increased in this group of patients. Furthermore, in the same cohort a positive correlation between galectin-3 and NT-pro BNP, and galectin-3 and LV mass were demonstrated. In addition, a negative correlation between galectin-3 and LV end-diastolic diameter was revealed. Conclusions: This study revealed that levels of galectin-3 were higher in patients with both T2D and HT, and correlated with LV mass, indicating the potential role of this biomarker for early detection of myocardial structural and functional alterations.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Structural myocardial alterations in diabetes and hypertension: the role of galectin-3
VL  - 52
IS  - 10
SP  - 1499
EP  - 1505
DO  - 10.1515/cclm-2014-0265
ER  - 

@article{
author = "Seferović, Jelena and Lalić, Nebojša M. and Floridi, Federico and Tesić, Milorad and Seferović, Petar M. and Giga, Vojislav and Lalić, Katarina and Jotić, Aleksandra and Jovičić, Snežana and Colak, Emina and Salerno, Gerardo and Cardelli, Patrizia and Di Somma, Salvatore",
year = "2014",
abstract = "Background: Galectin-3 is a protein widely distributed in the heart, brain and blood vessels, and has a regulatory role in inflammation, immunology and cancer. Many studies demonstrated that the increased level of galectin-3 is associated with progressive fibrosis and stiffening of the myocardium. The aim of this study was to investigate the role of galectin-3 in patients with type 2 diabetes (T2D) and/or arterial hypertension (HT). Methods: Study population included 189 patients, with no coronary artery disease, divided into three groups: group 1 (T2D), group 2 (T2D+HT), and group 3 (HT). All subjects underwent routine laboratory tests, as well as specific biomarkers assessment [galectin-3, glycosylated hemoglobin (HbA(1c)), N- terminal fragment B-type natriuretic peptide (NT-proBNP)]. Cardiological evaluation included physical examination, transthoracic tissue Doppler echocardiography and stress echocardiography. Results: The results of this study demonstrated significantly increased levels of galectin-3, blood glucose, and HbA(1c) in group 2. Also, echocardiographicaly, left ventricular (LV) diameters and IVS thickness were increased in this group of patients. Furthermore, in the same cohort a positive correlation between galectin-3 and NT-pro BNP, and galectin-3 and LV mass were demonstrated. In addition, a negative correlation between galectin-3 and LV end-diastolic diameter was revealed. Conclusions: This study revealed that levels of galectin-3 were higher in patients with both T2D and HT, and correlated with LV mass, indicating the potential role of this biomarker for early detection of myocardial structural and functional alterations.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Structural myocardial alterations in diabetes and hypertension: the role of galectin-3",
volume = "52",
number = "10",
pages = "1499-1505",
doi = "10.1515/cclm-2014-0265"
}

Seferović, J., Lalić, N. M., Floridi, F., Tesić, M., Seferović, P. M., Giga, V., Lalić, K., Jotić, A., Jovičić, S., Colak, E., Salerno, G., Cardelli, P.,& Di Somma, S.. (2014). Structural myocardial alterations in diabetes and hypertension: the role of galectin-3. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 52(10), 1499-1505.
https://doi.org/10.1515/cclm-2014-0265

Seferović J, Lalić NM, Floridi F, Tesić M, Seferović PM, Giga V, Lalić K, Jotić A, Jovičić S, Colak E, Salerno G, Cardelli P, Di Somma S. Structural myocardial alterations in diabetes and hypertension: the role of galectin-3. in Clinical Chemistry and Laboratory Medicine. 2014;52(10):1499-1505.
doi:10.1515/cclm-2014-0265 .

Seferović, Jelena, Lalić, Nebojša M., Floridi, Federico, Tesić, Milorad, Seferović, Petar M., Giga, Vojislav, Lalić, Katarina, Jotić, Aleksandra, Jovičić, Snežana, Colak, Emina, Salerno, Gerardo, Cardelli, Patrizia, Di Somma, Salvatore, "Structural myocardial alterations in diabetes and hypertension: the role of galectin-3" in Clinical Chemistry and Laboratory Medicine, 52, no. 10 (2014):1499-1505,
https://doi.org/10.1515/cclm-2014-0265 . .

TY  - THES
AU  - Jovičić, Snežana
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1005
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:7631/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44888079
UR  - http://nardus.mpn.gov.rs/123456789/3149
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3392
AB  - U kliničkoj praksi koristi se nekoliko skorova za procenu rizika od pojaverazličitih oblika kardiovaskularnih bolesti (KVB) koji se zasnivaju na multivarijabilnimregresionim jednačinama izvedenim iz rezultata praćenja različitih kohortnih grupa. Naosnovu prisustva tradicionalnih faktora rizika [hiperholesterolemija, hipertenzija, pol,starost, porodična istorija KVB, dijabetes i pušenje] definisanim algoritmima seizračunava apsolutni 10-godišnji rizik za koronarnu bolest srca (KBS) na osnovuFramingamskog rizik skora (FRS), 10-godišnji rizik od svih oblika KVB – tzv. „globalniKVB rizik“ (globalni FRS), kao i dugoročni (30-godišnji) rizik za KVB.Ateroskleroza je bolest uslovljena brojnim faktorima koju prati hroničnainflamacija niskog intenziteta i dislipidemija. Zahvaljujući velikom broju postojećihdokaza da C-reaktivni protein (CRP) snažno i nezavisno predviđa pojavukardiovaskularnih komplikacija, primena CRP-a u kliničkoj praksi definisana je odstrane nekoliko organizacija. Za proces ateroskleroze karakteristična je hroničnainflamacija gde su koncentracije CRP-a u cirkulaciji niže od granice detekcijekonvencionalnih testova. Određivanje tako niskih nivoa CRP-a zahteva testove savećom analitičkom osetljivošću, koji se označavaju kao visoko osetljivi (highsensitivity,hs), a na ovaj način određena koncentracija CRP-a kao „visoko osetljiviCRP“ (hsCRP). Takođe, postoje podaci i o drugim faktorima koji doprinose održavanjuinflamacije ili odražavaju intenzitet aterosklerotskih procesa i koji bi mogli daidentifikuju doprinos kardiovaskularnom riziku koji ne potiče od tradicionalnih faktorarizika, kao što su mokraćna kiselina, jačina glomerularne filtracije procenjena na osnovukoncentracije kreatinina ili cistatina C (eGFR), amino-terminalni pro-natriuretičkipeptid tipa B (NT-proBNP), srčani troponin (cTn).Cilj rada bio je da se ispita da li postoji povezanost između hsCRP-a,ustanovljenog biomarkera proaterogenog metaboličkog stanja, i drugih biomarkerainflamacije [serumski amiloid A (SAA), fibrinogen, α1-kiseli glikoprotein (A1AGP),haptoglobin, C3 i C4 komponente komplementa), metabolizma lipida [ukupan, HDL,non-HDL i LDL holesterol, trigliceridi, apolipoprotein A-I (apo A-I), apolipoprotein B(apo B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin, cistatin C (Cys-C), procenjenajačina glomerularne filtracije (eGFR)] i srčane funkcije (NT-proBNP, cTnT), koji bimogli da unaprede procenu kardiovaskularnog rizika u primarnoj prevenciji. Ispitane sui analitičke karakteristike i klinička efikasnost metode visoke osetljivosti koja sekoristila za određivanje hsCRP-a. Faktorskom analizom ispitana je priroda uticajasvakog pojedinačnog biomarkera na kardiovaskularni rizik i eventualna povezanost savrednostima hsCRP-a, grupisanje ispitivanih biomarkera povezanih sa aterosklerozom iinflamacijom slabog intenziteta, kao i povezanost dobijenih faktora sa vrednostimahsCRP, kategorizacijom 10-godišnjeg rizika na osnovu FRS i globalnog FRS, kao i saklasifikacijom 30-godišnjeg rizika...
AB  - Several risk score algorithms for cardiovascular risk assessment based onmultivariable regression equations derived from different cohorts are being used inclinical practice. According to presence of traditional risk factors [hypercholesterolemia,hypertension, gender, age, family history of premature cardiovascular disease (CVD),diabetes and cigarette smoking], absolute 10-year risk for coronary heart disease (CHD)according to Framingham risk score (FRS), 10-year risk for cardiovascular disease ingeneral – „global CVD risk“ using global FRS, and long term (30-year) CVD risk arebeing calculated.Atherosclerosis is a disease conditioned with multiple factors followed bychronic low-grade inflammation and dyslipidemia. Thanks to substantial evidence thatC-reactive protein (CRP) strongly and independently predicts cardiovascularcomplications, the use of CRP in clinical practice is recommended by severalinstitutions. Atherosclerosis process is characterized with chronic inflammation wherecirculating CRP concentrations are lower than limit of detection of conventional assays.For measuring such low CRP levels high-sensitivity (hsCRP) assays have beendeveloped. Also, there are evidence of other factors, contributing to and maintaining theintensity of atherosclerotic processes, which might identify cardiovascular riskcontribution not originated from traditional risk factors. These are uric acid, estimatedglomerular filtration rate (eGFR) based on creatinine or cystatin C, amino-terminal pro-B-type natriuretic peptide (NT-proBNP), cardiac troponin (cTn).The aim of this study was to examine whether there is association betweenhsCRP, as the established marker of proaterogenic metabolic state, and other biomarkersof inflammation [serum amyloid A (SAA), fibrinogen, α1-acid glycoprotein (A1AGP),haptoglobin, C3 and C4 complement components), lipid metabolism [total, HDL, non-HDL and LDL cholesterol, triglycerides, apolipoprotein A-I (apo A-I), apolipoprotein B(apo B), lipoprotein (a) (Lp(a))], renal [creatinine, cystatin C (Cys-S), estimatedglomerular filtration rate (eGFR)] and cardiac function (NT-proBNP, cTnT), whichmight promote cardiovascular risk assessment in primary prevention. The analyticalperformance and clinical efficacy of high sensitivity method used for CRPdetermination were also evaluated. Using factor analysis, the nature of influence ofevery single examined biomarker on cardiovascular risk and their possible connection tohsCRP values, also clustering of examined biomarkers associated with atherosclerosisand low-grade inflammation, as well as relations of identified factors with hsCRPvalues, 10-year risk categorization based on FRS and global FRS, and 30-year riskclassification, were analyzed.The examined population were 242 healthy volunteers, 100 men and 142women, 20–80 years old. They were free of diabetes mellitus and of any known cardiac,renal, hepatic and rheumatic disease, and were not taking any prescribed medication.Information about their age, smoking habits, body weight and height, physical activity, family history of CVD, aspirin intake and, in case of women, if they were taking anyoral contraceptives, were obtained through questionnaire. Blood pressure was measuredprior to venipuncture...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Faktorska analiza i povezanost lipidnih, inflamatornih, srčanih i bubrežnih biomarkera sa C-reaktivnim proteinom u kategorizaciji kardiovaskularnog rizika
T1  - Factor analysis and association of lipid, inflammatory, cardiac and renal biomarkers with creactive protein in cardiovascular risk categorization
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3149
ER  - 

@phdthesis{
author = "Jovičić, Snežana",
year = "2013",
abstract = "U kliničkoj praksi koristi se nekoliko skorova za procenu rizika od pojaverazličitih oblika kardiovaskularnih bolesti (KVB) koji se zasnivaju na multivarijabilnimregresionim jednačinama izvedenim iz rezultata praćenja različitih kohortnih grupa. Naosnovu prisustva tradicionalnih faktora rizika [hiperholesterolemija, hipertenzija, pol,starost, porodična istorija KVB, dijabetes i pušenje] definisanim algoritmima seizračunava apsolutni 10-godišnji rizik za koronarnu bolest srca (KBS) na osnovuFramingamskog rizik skora (FRS), 10-godišnji rizik od svih oblika KVB – tzv. „globalniKVB rizik“ (globalni FRS), kao i dugoročni (30-godišnji) rizik za KVB.Ateroskleroza je bolest uslovljena brojnim faktorima koju prati hroničnainflamacija niskog intenziteta i dislipidemija. Zahvaljujući velikom broju postojećihdokaza da C-reaktivni protein (CRP) snažno i nezavisno predviđa pojavukardiovaskularnih komplikacija, primena CRP-a u kliničkoj praksi definisana je odstrane nekoliko organizacija. Za proces ateroskleroze karakteristična je hroničnainflamacija gde su koncentracije CRP-a u cirkulaciji niže od granice detekcijekonvencionalnih testova. Određivanje tako niskih nivoa CRP-a zahteva testove savećom analitičkom osetljivošću, koji se označavaju kao visoko osetljivi (highsensitivity,hs), a na ovaj način određena koncentracija CRP-a kao „visoko osetljiviCRP“ (hsCRP). Takođe, postoje podaci i o drugim faktorima koji doprinose održavanjuinflamacije ili odražavaju intenzitet aterosklerotskih procesa i koji bi mogli daidentifikuju doprinos kardiovaskularnom riziku koji ne potiče od tradicionalnih faktorarizika, kao što su mokraćna kiselina, jačina glomerularne filtracije procenjena na osnovukoncentracije kreatinina ili cistatina C (eGFR), amino-terminalni pro-natriuretičkipeptid tipa B (NT-proBNP), srčani troponin (cTn).Cilj rada bio je da se ispita da li postoji povezanost između hsCRP-a,ustanovljenog biomarkera proaterogenog metaboličkog stanja, i drugih biomarkerainflamacije [serumski amiloid A (SAA), fibrinogen, α1-kiseli glikoprotein (A1AGP),haptoglobin, C3 i C4 komponente komplementa), metabolizma lipida [ukupan, HDL,non-HDL i LDL holesterol, trigliceridi, apolipoprotein A-I (apo A-I), apolipoprotein B(apo B), lipoprotein (a) (Lp(a))], bubrežne [kreatinin, cistatin C (Cys-C), procenjenajačina glomerularne filtracije (eGFR)] i srčane funkcije (NT-proBNP, cTnT), koji bimogli da unaprede procenu kardiovaskularnog rizika u primarnoj prevenciji. Ispitane sui analitičke karakteristike i klinička efikasnost metode visoke osetljivosti koja sekoristila za određivanje hsCRP-a. Faktorskom analizom ispitana je priroda uticajasvakog pojedinačnog biomarkera na kardiovaskularni rizik i eventualna povezanost savrednostima hsCRP-a, grupisanje ispitivanih biomarkera povezanih sa aterosklerozom iinflamacijom slabog intenziteta, kao i povezanost dobijenih faktora sa vrednostimahsCRP, kategorizacijom 10-godišnjeg rizika na osnovu FRS i globalnog FRS, kao i saklasifikacijom 30-godišnjeg rizika..., Several risk score algorithms for cardiovascular risk assessment based onmultivariable regression equations derived from different cohorts are being used inclinical practice. According to presence of traditional risk factors [hypercholesterolemia,hypertension, gender, age, family history of premature cardiovascular disease (CVD),diabetes and cigarette smoking], absolute 10-year risk for coronary heart disease (CHD)according to Framingham risk score (FRS), 10-year risk for cardiovascular disease ingeneral – „global CVD risk“ using global FRS, and long term (30-year) CVD risk arebeing calculated.Atherosclerosis is a disease conditioned with multiple factors followed bychronic low-grade inflammation and dyslipidemia. Thanks to substantial evidence thatC-reactive protein (CRP) strongly and independently predicts cardiovascularcomplications, the use of CRP in clinical practice is recommended by severalinstitutions. Atherosclerosis process is characterized with chronic inflammation wherecirculating CRP concentrations are lower than limit of detection of conventional assays.For measuring such low CRP levels high-sensitivity (hsCRP) assays have beendeveloped. Also, there are evidence of other factors, contributing to and maintaining theintensity of atherosclerotic processes, which might identify cardiovascular riskcontribution not originated from traditional risk factors. These are uric acid, estimatedglomerular filtration rate (eGFR) based on creatinine or cystatin C, amino-terminal pro-B-type natriuretic peptide (NT-proBNP), cardiac troponin (cTn).The aim of this study was to examine whether there is association betweenhsCRP, as the established marker of proaterogenic metabolic state, and other biomarkersof inflammation [serum amyloid A (SAA), fibrinogen, α1-acid glycoprotein (A1AGP),haptoglobin, C3 and C4 complement components), lipid metabolism [total, HDL, non-HDL and LDL cholesterol, triglycerides, apolipoprotein A-I (apo A-I), apolipoprotein B(apo B), lipoprotein (a) (Lp(a))], renal [creatinine, cystatin C (Cys-S), estimatedglomerular filtration rate (eGFR)] and cardiac function (NT-proBNP, cTnT), whichmight promote cardiovascular risk assessment in primary prevention. The analyticalperformance and clinical efficacy of high sensitivity method used for CRPdetermination were also evaluated. Using factor analysis, the nature of influence ofevery single examined biomarker on cardiovascular risk and their possible connection tohsCRP values, also clustering of examined biomarkers associated with atherosclerosisand low-grade inflammation, as well as relations of identified factors with hsCRPvalues, 10-year risk categorization based on FRS and global FRS, and 30-year riskclassification, were analyzed.The examined population were 242 healthy volunteers, 100 men and 142women, 20–80 years old. They were free of diabetes mellitus and of any known cardiac,renal, hepatic and rheumatic disease, and were not taking any prescribed medication.Information about their age, smoking habits, body weight and height, physical activity, family history of CVD, aspirin intake and, in case of women, if they were taking anyoral contraceptives, were obtained through questionnaire. Blood pressure was measuredprior to venipuncture...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Faktorska analiza i povezanost lipidnih, inflamatornih, srčanih i bubrežnih biomarkera sa C-reaktivnim proteinom u kategorizaciji kardiovaskularnog rizika, Factor analysis and association of lipid, inflammatory, cardiac and renal biomarkers with creactive protein in cardiovascular risk categorization",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3149"
}

Jovičić, S.. (2013). Faktorska analiza i povezanost lipidnih, inflamatornih, srčanih i bubrežnih biomarkera sa C-reaktivnim proteinom u kategorizaciji kardiovaskularnog rizika. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_3149

Jovičić S. Faktorska analiza i povezanost lipidnih, inflamatornih, srčanih i bubrežnih biomarkera sa C-reaktivnim proteinom u kategorizaciji kardiovaskularnog rizika. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_3149 .

Jovičić, Snežana, "Faktorska analiza i povezanost lipidnih, inflamatornih, srčanih i bubrežnih biomarkera sa C-reaktivnim proteinom u kategorizaciji kardiovaskularnog rizika" in Универзитет у Београду (2013),
https://hdl.handle.net/21.15107/rcub_nardus_3149 .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Kangrga, Ranka
AU  - Beletić, Anđelo
AU  - Mirković, Duško
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1656
AB  - Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L).
AB  - Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience
T1  - Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo
VL  - 31
IS  - 4
SP  - 347
EP  - 357
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1656
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Kangrga, Ranka and Beletić, Anđelo and Mirković, Duško and Majkić-Singh, Nada",
year = "2012",
abstract = "Determination of 25-hydroxyvitamin D [25(OH)D] represents a unique challenge, considering its lipophilic nature. Considering the widespread prevalence of vitamin D deficiency, which leads to increasing number of requests for 25(OH)D determination, immunoassay measurements adjusted to automated analyzers are being developed. Because of the variability among assays, it is often difficult to monitor vitamin D status and supplementation. The aim of this study was to compare the results of two immunoassays with high performance liquid chromatography with ultraviolet detection (HPLC-UV). Also, the aim was to estimate vitamin D status, since up to date the prevalence of vitamin D deficiency in Serbia was not examined. We have evaluated analytical characteristics of two automated immunoassays for 25(OH)D determination, from Roche (Cobas® e601) and Abbott (Architect). For comparison studies we used HPLC analysis of 25-(OH)-Vitamin D3/D2 from Chromsystems (Waters isocratic system). In order to estimate vitamin D status in general population, we have searched the database of the laboratory information system and analyzed the data from 533 patients whose 25(OH)D was determined together with intact parathyroid hormone (iPTH). For imprecision assessment, four serum pools were prepared with following 25(OH)D concentrations: 35 nmol/L, ?50 nmol/L, ?75 nmol/L and ?125 nmol/L. Obtai ned CVs for Roche method were 1.5-2.8% for within-run and 4.0-6.7% for between-run imprecision. For Abbott method, CVs were 0.7-4.4% for withinrun and 3.8-7.2% for between-run imprecision. Inaccuracy was analyzed with commercial control sera. Obtained deviations from target value were 2.1% for Roche assay and 1.3-1.5% for Abbott method, and were not statistically significant (P>0.05). Comparison of Roche and HPLC-UV methods using Passing-Bablok regression analysis gave the following equation for the regression line y=0.937x+9.518 (r=0.739; n=97) and the regression line equation from the comparison of Abbott and HPLC-UV methods was y=0.745x+10.343 (r=0.793; n=97). Mean difference and SD for Bland-Altman plot were -4.5 nmol/L and 21.75 nmo/L, respectively for Roche method and 6.4 nmol/L and 18.8 nmol/L, respectively for Abbott. Statistical analysis (Chi-square test) of frequency distribution among different vitamin D status categories ( lt 25 nmol/L severe deficiency, 25-50 nmol/L deficiency, 50-75 nmol/L insufficiency and >75 nmol/L sufficiency) showed that the frequency distribution obtained with Abbott method was significantly different from the distribution of the HPLC results, in contrast to Roche results frequency distribution which did not differ significantly. Also, statistical analysis of the agreement between the three methods for each vitamin D status category showed that results of both Roche and Abbott methods were significantly higher than HPLC in the two deficiency categories (P=0.005 for Roche, P=0.0407 for Abbott), and in the sufficiency category Abbott method significantly underestimated concentration of 25(OH)D compared to HPLC results (P lt 0.0001). Median population values of 25(OH)D and iPTH were 41.8 nmol/L and 76.6 ng/L, respectively. ANOVA analyses showed significant (P lt 0.05) decrease in iPTH and Ca2+ concentrations across the 25(OH)D concentration categories. Stepwise multiple linear regression analysis indicated independent correlation of iPTH with 25(OH)D concentration (b=-0.290, P=0.0008). Also, one-way ANOVA with Student-Newman-Keuls test demonstrated that 25(OH)D concentrations measured in summer and autumn were significantly (P lt 0.001) higher compared to those determined in winter and spring. Despite acceptable imprecision and inaccuracy of both examined methods, results obtained with them did not correlate well with HPLC-UV (r lt 0.9), which was used as a reference. However, methods showed satisfactory ability to classify patients into vitamin D status categories, which is important for diagnosis of vitamin D deficiency and therapy follow-up. About two thirds (68.5%) of the examined population had vitamin D deficiency (25(OH)D lt 50 nmol/L) and only 8% had sufficient 25(OH)D concentration (>75 nmol/L)., Određivanje 25-hidroksivitamina D [25(OH)D] predstavlja jedinstven izazov, s obzirom da je visoko lipofilno jedinjenje. Visoka prevalencija deficijencije vitamina D uzrok je povećanja broja zahteva za određivanjem 25(OH)D, zbog čega se razvijaju imunohemijske metode prilagođene automatizovanim sistemima. Često je teško pratiti status vitamina D i suplementaciju zbog varijabilnosti između testova. Cilj ove studije bio je da se uporede rezultati dve imunohemijske metode sa tečnom hromatografijom visoke efikasnosti sa detekcijom u ultraljubičastom delu spektra (HPLC-UV). Takođe, cilj je bio i procena statusa vitamina D, pošto do sada nije ispitivana prevalencija deficijencije vitamina D u Srbiji. Ispitivane su karakteristike dve imunohemijske metode za određivanje 25(OH)D, proizvođača Roche (analizator Cobas® e601) i Abbott (na analizatoru Architect). Metode su poređene sa rezultatima HPLC analize korišćenjem 25-(OH)-Vitamin D3/D2 reagenasa firme Chromsystems (Waters izokratski sistem). Da bi se procenio status vitamina D u opštoj populaciji, pretražena je baza podataka laboratorijskog informacionog sistema i analizirani su rezultati 533 pacijenata kojima je određen 25(OH)D zajedno sa intaktnim paratiroidnim hormonom (iPTH). Pripremljena su četiri serumska pool-a sa koncentracijama 25(OH)D ? 35 nmol/L, ?50 nmol/L, ?75 nmol/L i ?125 nmol/L za procenu nepreciznosti imunohemijskih određivanja. Dobijeni koeficijenti varijacije za Roche metodu su se kretali u opsegu 1,5-2,8% u seriji i 4,0-6,7% između serija. Za Abbott metodu su koficijenti varijacije iznosili 0,7-4,4% u seriji i 3,8-7,2% između serija. Netačnost je ispitivana pomoću komercijalnih kontrolnih uzoraka. Dobijena odstupanja od deklarisane vrednosti su iznosila 2,1% za Roche i 1,3-1,5% za Abbott, i nisu bila statistički značajna (P>0,05). Poređenjem Roche i HPLC-UV metoda pomoću Passing-Bablok regresione analize dobijena je sledeća regresiona jednačina y=0,937x+9,518 (r=0,739; n=97), dok regresiona jednačina dobijena poređenjem Abbott i HPLC-UV metoda glasi y=0,745x+10,343 (r=0,793; n=97). Srednja vrednost razlika na Bland-Altman dijagramu razlika i standardna devijacija su iznosile -4,5 nmol/L i 21,75 nmo/L, redom, za Roche metodu i 6,4 nmol/L i 18,8 nmol/L, re dom, za Abbott metodu. Statistička analiza (Chi-kvadrat test) distribucije frekvencija među različitim kategorijama statusa vitamina D ( lt 25 nmol/L teška deficijencija, 25-50 nmol/L deficijencija, 50-75 nmol/L insuficijencija i >75 nmol/L preporučena koncentracija) je pokazala da je distribucija frekvencija dobijena Abbott metodom značajno različita od distribucije HPLC rezultata, za razliku od ras po dele frekvencija dobijene Roche metodom koja se nije značajno razlikovala. Takođe, statistička analiza slaganja između ispitivane tri metode u svakoj od kategorija statusa vitamina D je pokazala da su rezultati i Roche i Abbott metoda značajno veći od HPLC-UV u kategorijama deficijencije vitamina D (P=0,005 za Roche; P=0,0407 za Abbott), i u kategoriji sa preporučenom koncentracijom vitamina D Abbott metoda je značajno potcenjivala koncentraciju 25(OH)D u poređenju sa HPLC rezultatima (P lt 0,0001). Medijana za 25(OH)D u ispitivanoj populaciji bila je 41,8 nmol/L, i 76,6 za iPTH. ANOVA analiza je pokazala značajan pad (P lt 0,05) koncentracija iPTH i jonizovanog kalcijuma između kategorija koncentracija 25(OH)D. Multiplomlinearnom regresionom analizom utvrđena je ne zavisna korelacija između koncentracija iPTH i 25(OH)D (b =-0,290; P=0,0008). Takođe, ANOVA za jedan kriterijum klasifikacije sa Student-Newman-Keuls testom je pokazala da su koncentracije 25(OH)D određene u leto i jesen značajno više (P lt 0,001) u poređenju sa onima određenim u zimu ili proleće. Uprkos prihvatljivoj nepreciznosti i netačnosti obe ispitivane imunohemijske metode, dobijeni rezultati nisu u zadovoljavajućoj korelaciji sa HPLC-UV metodom (r lt 0,9), koja je korišćena kao referentna u ovom slučaju. Uprkos ovoj činjenici, metode su pokazale zadovoljavajuću sposobnost klasifikacije pacijenata u kategorije statusa vitamina D, što je važno za dijagnozu deficijencije vitamina D i praćenje terapije. Oko dve trećine (68,5%) ispitivane populacije je imalo deficijenciju vitamina D (25(OH)D lt 50 nmol/L) i samo 8% je imalo preporučenu koncentraciju 25(OH)D (>75 nmol/L).",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience, Poređenje tri različite metode za određivanje 25(OH)-vitamina D i statusa vitamina D u opštoj populaciji - srpsko iskustvo",
volume = "31",
number = "4",
pages = "347-357",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1656"
}

Jovičić, S., Ignjatović, S., Kangrga, R., Beletić, A., Mirković, D.,& Majkić-Singh, N.. (2012). Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656

Jovičić S, Ignjatović S, Kangrga R, Beletić A, Mirković D, Majkić-Singh N. Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience. in Journal of Medical Biochemistry. 2012;31(4):347-357.
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

Jovičić, Snežana, Ignjatović, Svetlana, Kangrga, Ranka, Beletić, Anđelo, Mirković, Duško, Majkić-Singh, Nada, "Comparison of three different methods for 25(OH)-vitamin D determination and vitamin D status in general population: Serbian experience" in Journal of Medical Biochemistry, 31, no. 4 (2012):347-357,
https://hdl.handle.net/21.15107/rcub_farfar_1656 .

TY  - JOUR
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1667
AB  - Vitamin D is not technically a vitamin, since it is not an essential dietary factor. It is rather a prohormone produced photochemically in the skin from 7-dehydrocholesterol. Vitamin D and its metabolites may be categorized as either cholecalciferols or ergocalciferols. Cholecalciferol (vitamin D3) is the parent compound of the naturally occurring family and is produced in the skin from 7-dehydrocholesterol on exposure to the ultraviolet B portion of sunlight. Vitamin D2 (ergocalciferol), the parent compound of the other family, is manufactured by irradiation of ergosterol produced by yeasts and its potency is less than one-third of vitamin D3's potency. The steps in the vitamin D endocrine system include the following: 1) the photoconversion of 7-dehydrocholesterol to vitamin D3 in the skin or dietary intake of vitamin D3; 2) metabolism of vitamin D3 by the liver to 25-hydroxyvitamin-D3 [25(OH)D3], the major form of vitamin D circulating in the blood compartment; 3) conversion of 25(OH)D3 by the kidney (functioning as an endocrine gland) to the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 ]; 4) systemic transport of the dihydroxylated metabolite 1,25(OH)2D3 to distal target organs; and 5) binding of 1,25(OH)2D3 to a nuclear receptor (VDR) at target organs, followed by generation of appropriate biological responses. The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes. Six cytochrome P450 (CYP) isoforms have been shown to hydroxylate vitamin D. Four of these, CYP27A1, CYP2R1, CYP3A4 and CYP2J3, are candidates for the enzyme vitamin D 25-hydroxylase that is involved in the first step of activation. The highly regulated, renal enzyme 25-hydroxyvitamin D-1a-hydro xylase contains the component CYP27B1, which completes the activation pathway to the hormonal form 1,25(OH)2D3. A five-step inactivation pathway from 1,25(OH)2D3 to calcitroic acid is attributed to a single multifunctional CYP, CYP24A1, which is transcriptionally induced in vitamin D target cells by the action of 1,25(OH)2D3. An additional key component in the operation of the vitamin D endocrine system is the plasma vitamin D binding protein (DBP), which carries vitamin D3 and its metabolites to their metabolism and target organs. DBP is a specific, high-affinity transport protein. It is synthesized by the liver and circulates in great excess, with fewer than 5% of the binding sites normally occupied. 1,25(OH)2D3, acts as a ligand for a nuclear transcription factor, vitamin D receptor - VDR, which like all other nuclear receptors, regulates gene transcription and cell function. The widespread presence of VDR, and the key activating (1a-hydroxylase, CYP27B1) and inactivating (24-hydroxylase, CYP24A1) enzymes in most mammalian cells means that the cells in these tissues have the potential to produce biological responses, depending on the availability of appropriate amounts of vitamin D3. Thanks to this widespread presence of elements of vitamin D endocrine system, its biological features are being recognized outside bone tissue, i.e. calcium and phosphate metabolism.
AB  - Vitamin D nije pravi vitamin, odnosno nije esencijalni dijetetski faktor, već je pre prohormon koji nastaje fotohemijskom reakcijom u koži iz 7-dehidroholesterola. Vita min D i njegovi metaboliti mogu da se kategorizuju kao holekalciferoli ili ergokalciferoli. Holekalciferol (vitamin D3) je polazno jedinjenje za familiju koja se nalazi u prirodi i produkuje se u koži iz 7-dehidroholesterola pri izlaganju ultraljubičastom B delu spektra sunčeve svetlosti. Vitamin D2 (ergokalciferol), polazno jedinjenje druge familije, nastaje radijacijom ergosterola koga produkuju kvasci i ima samo jednu trećinu aktivnosti vitamina D3. Faze u endokrinom sistemu vitamina D su: 1) fotokonverzija 7-dehidroholesterola u vitamin D3 u koži ili unos vitamina D3-hranom; 2) metabolizam vitamina D3 u jetri do 25-hidroksivitamina D3 [25(OH)D3], glavnog oblika vitamina D u cirkulaciji; 3) konverzija 25(OH)D3 u bubregu (koji ovde funkcioniše kao endokrina žlezda) do hormona 1,25-dihidroksivitamin D3 [1,25(OH)2D3]; 4) sistemski transport dihidroksi-metabolita do distalnih ciljnih organa; i 5) vezivanje 1,25(OH)2D3 za nuklearni receptor (VDR) u ciljnim organima, što prati odgovarajući biološki odgovor. Aktivacija vitamina D do hormonskog oblika je posredovana citohrom P450 enzimima. Pokazano je da šest izoformi citohroma P450 (CYP) učestvuje u hidroksilaciji vitamina D. Za četiri od njih, CYP27A1, CYP2R1, CYP3A4 i CYP2J3, se pretpostavlja da imaju aktivnost 25-hidroksilaze koja uče s tvuje u prvom koraku aktivacije. Renalni enzim, 25-hidroksivitamin D-1a-hidroksilaza sa strogo regulisanom aktivnošću, predstavlja CYP27B1, koji završava aktivaciju do hormonskog oblika 1,25(OH)2D3. Proces inaktivacije, koji se sastoji iz pet stupnjeva od 1,25(OH)2D3 do kalcitroične kiseline, obavlja jedan multifunkcionalni CYP, CYP24A1, čija je transkripcija indukovana u ciljnim ćelija carbonma dejstva vitamina D posredstvom 1,25(OH)2D3. Dodatna ključna komponenta u dejstvu vitamin D endokrinog sistema je vitamin D vezujući protein u plazmi (DBP), koji transportuje vitamin D3 i njegove metabolite do ciljnih i organa gde se odvija njihov metabolizam. DBP je specifičan transportni protein velikog afiniteta. Sintetiše se u jetri i cirkuliše u velikom višku, sa zasićenjem vezujućih mesta manjim od 5%. 1,25(OH)2D3 deluje kao ligand nuklearnog transkripcionog faktora, VDR, koji reguliše transkripciju gena i funkciju ćelija. široka rasprostranjenost VDR i ključnih enizma aktivacije (1a-hidroksilaza, CYP27B1) i inaktivacije (24-hidroksilaza, CYP24A1) u većini ćelija sisara znači da ćelije u ovim tkivima imaju potencijal za produkovanje bioloških odgovora, zavisno od raspoloživosti dovoljnih ko li čina vitamina D3. Zahvaljujući rasprostranjenosti elemenata endokrinog sistema vitamina D, njegove biološke oso bi ne se prepoznaju i izvan koštanog sistema, odnosno metabolizma kalcijuma i fosfora.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Biochemistry and metabolism of vitamin D
T1  - Biohemija i metabolizam vitamina D
VL  - 31
IS  - 4
SP  - 309
EP  - 315
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1667
ER  - 

@article{
author = "Jovičić, Snežana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2012",
abstract = "Vitamin D is not technically a vitamin, since it is not an essential dietary factor. It is rather a prohormone produced photochemically in the skin from 7-dehydrocholesterol. Vitamin D and its metabolites may be categorized as either cholecalciferols or ergocalciferols. Cholecalciferol (vitamin D3) is the parent compound of the naturally occurring family and is produced in the skin from 7-dehydrocholesterol on exposure to the ultraviolet B portion of sunlight. Vitamin D2 (ergocalciferol), the parent compound of the other family, is manufactured by irradiation of ergosterol produced by yeasts and its potency is less than one-third of vitamin D3's potency. The steps in the vitamin D endocrine system include the following: 1) the photoconversion of 7-dehydrocholesterol to vitamin D3 in the skin or dietary intake of vitamin D3; 2) metabolism of vitamin D3 by the liver to 25-hydroxyvitamin-D3 [25(OH)D3], the major form of vitamin D circulating in the blood compartment; 3) conversion of 25(OH)D3 by the kidney (functioning as an endocrine gland) to the hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 ]; 4) systemic transport of the dihydroxylated metabolite 1,25(OH)2D3 to distal target organs; and 5) binding of 1,25(OH)2D3 to a nuclear receptor (VDR) at target organs, followed by generation of appropriate biological responses. The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes. Six cytochrome P450 (CYP) isoforms have been shown to hydroxylate vitamin D. Four of these, CYP27A1, CYP2R1, CYP3A4 and CYP2J3, are candidates for the enzyme vitamin D 25-hydroxylase that is involved in the first step of activation. The highly regulated, renal enzyme 25-hydroxyvitamin D-1a-hydro xylase contains the component CYP27B1, which completes the activation pathway to the hormonal form 1,25(OH)2D3. A five-step inactivation pathway from 1,25(OH)2D3 to calcitroic acid is attributed to a single multifunctional CYP, CYP24A1, which is transcriptionally induced in vitamin D target cells by the action of 1,25(OH)2D3. An additional key component in the operation of the vitamin D endocrine system is the plasma vitamin D binding protein (DBP), which carries vitamin D3 and its metabolites to their metabolism and target organs. DBP is a specific, high-affinity transport protein. It is synthesized by the liver and circulates in great excess, with fewer than 5% of the binding sites normally occupied. 1,25(OH)2D3, acts as a ligand for a nuclear transcription factor, vitamin D receptor - VDR, which like all other nuclear receptors, regulates gene transcription and cell function. The widespread presence of VDR, and the key activating (1a-hydroxylase, CYP27B1) and inactivating (24-hydroxylase, CYP24A1) enzymes in most mammalian cells means that the cells in these tissues have the potential to produce biological responses, depending on the availability of appropriate amounts of vitamin D3. Thanks to this widespread presence of elements of vitamin D endocrine system, its biological features are being recognized outside bone tissue, i.e. calcium and phosphate metabolism., Vitamin D nije pravi vitamin, odnosno nije esencijalni dijetetski faktor, već je pre prohormon koji nastaje fotohemijskom reakcijom u koži iz 7-dehidroholesterola. Vita min D i njegovi metaboliti mogu da se kategorizuju kao holekalciferoli ili ergokalciferoli. Holekalciferol (vitamin D3) je polazno jedinjenje za familiju koja se nalazi u prirodi i produkuje se u koži iz 7-dehidroholesterola pri izlaganju ultraljubičastom B delu spektra sunčeve svetlosti. Vitamin D2 (ergokalciferol), polazno jedinjenje druge familije, nastaje radijacijom ergosterola koga produkuju kvasci i ima samo jednu trećinu aktivnosti vitamina D3. Faze u endokrinom sistemu vitamina D su: 1) fotokonverzija 7-dehidroholesterola u vitamin D3 u koži ili unos vitamina D3-hranom; 2) metabolizam vitamina D3 u jetri do 25-hidroksivitamina D3 [25(OH)D3], glavnog oblika vitamina D u cirkulaciji; 3) konverzija 25(OH)D3 u bubregu (koji ovde funkcioniše kao endokrina žlezda) do hormona 1,25-dihidroksivitamin D3 [1,25(OH)2D3]; 4) sistemski transport dihidroksi-metabolita do distalnih ciljnih organa; i 5) vezivanje 1,25(OH)2D3 za nuklearni receptor (VDR) u ciljnim organima, što prati odgovarajući biološki odgovor. Aktivacija vitamina D do hormonskog oblika je posredovana citohrom P450 enzimima. Pokazano je da šest izoformi citohroma P450 (CYP) učestvuje u hidroksilaciji vitamina D. Za četiri od njih, CYP27A1, CYP2R1, CYP3A4 i CYP2J3, se pretpostavlja da imaju aktivnost 25-hidroksilaze koja uče s tvuje u prvom koraku aktivacije. Renalni enzim, 25-hidroksivitamin D-1a-hidroksilaza sa strogo regulisanom aktivnošću, predstavlja CYP27B1, koji završava aktivaciju do hormonskog oblika 1,25(OH)2D3. Proces inaktivacije, koji se sastoji iz pet stupnjeva od 1,25(OH)2D3 do kalcitroične kiseline, obavlja jedan multifunkcionalni CYP, CYP24A1, čija je transkripcija indukovana u ciljnim ćelija carbonma dejstva vitamina D posredstvom 1,25(OH)2D3. Dodatna ključna komponenta u dejstvu vitamin D endokrinog sistema je vitamin D vezujući protein u plazmi (DBP), koji transportuje vitamin D3 i njegove metabolite do ciljnih i organa gde se odvija njihov metabolizam. DBP je specifičan transportni protein velikog afiniteta. Sintetiše se u jetri i cirkuliše u velikom višku, sa zasićenjem vezujućih mesta manjim od 5%. 1,25(OH)2D3 deluje kao ligand nuklearnog transkripcionog faktora, VDR, koji reguliše transkripciju gena i funkciju ćelija. široka rasprostranjenost VDR i ključnih enizma aktivacije (1a-hidroksilaza, CYP27B1) i inaktivacije (24-hidroksilaza, CYP24A1) u većini ćelija sisara znači da ćelije u ovim tkivima imaju potencijal za produkovanje bioloških odgovora, zavisno od raspoloživosti dovoljnih ko li čina vitamina D3. Zahvaljujući rasprostranjenosti elemenata endokrinog sistema vitamina D, njegove biološke oso bi ne se prepoznaju i izvan koštanog sistema, odnosno metabolizma kalcijuma i fosfora.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Biochemistry and metabolism of vitamin D, Biohemija i metabolizam vitamina D",
volume = "31",
number = "4",
pages = "309-315",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1667"
}

Jovičić, S., Ignjatović, S.,& Majkić-Singh, N.. (2012). Biochemistry and metabolism of vitamin D. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(4), 309-315.
https://hdl.handle.net/21.15107/rcub_farfar_1667

Jovičić S, Ignjatović S, Majkić-Singh N. Biochemistry and metabolism of vitamin D. in Journal of Medical Biochemistry. 2012;31(4):309-315.
https://hdl.handle.net/21.15107/rcub_farfar_1667 .

Jovičić, Snežana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Biochemistry and metabolism of vitamin D" in Journal of Medical Biochemistry, 31, no. 4 (2012):309-315,
https://hdl.handle.net/21.15107/rcub_farfar_1667 .