TY  - JOUR
AU  - Bećarević, Mirjana
AU  - Jovičić, Snežana
AU  - Ignjatović, Svetlana
AU  - Mirković, Duško
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4320
AB  - Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings.
AB  - Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data
T1  - Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci
VL  - 41
IS  - 4
SP  - 506
EP  - 512
DO  - 10.5937/JOMB0-35642
ER  - 

@article{
author = "Bećarević, Mirjana and Jovičić, Snežana and Ignjatović, Svetlana and Mirković, Duško",
year = "2022",
abstract = "Background: The investigation of the prevalence of the IgG and the IgM isotypes of anticardiolipin (aCL) and antib2glycoprotein I (ab2gpI) Abs in healthy Serbian middle-aged subjects was the main goal of our study. In addition, we analyzed the potential associations of above-mentioned Abs with serum proteins and lipids/lipoproteins. Methods: Forty healthy subjects were included in our study. Obesity (BMI 30 kg/m2) was present in 8/40 (20%) subjects. Titers of analyzed Abs were measured by ELISA. Results: The prevalence of IgG and IgM ab2gpI Abs was 5% and 12.5%, respectively, while the prevalence of IgM aCL was 10%. The IgG ab2gpI Abs were significantly different between subjects with normal triglycerides levels and those with hypertriglyceridemia (Mann-Whitney, P = 0.014). The significant difference in hsCRP concentrations was observed between subjects with the increased levels of the IgM isotype of aCL Abs and those with normal IgM aCL values (Mann-Whitney, P = 0.028). Conclusions: Dyslipidemia and BMI ≥30 were associated with aPL Abs and therefore, the correction of BMI and lipid status might be beneficial in reduction or elimination of predisposing factors that might trigger thrombotic events in otherwise healthy middle-aged subjects. Larger national study is necessary to confirm our findings., Uvod: Analiza prevalentnosti IgG i IgM izotipa antikardiolip- inskih (aCL) i anti- b2glikoprotein I (ab2gpI) At kod zdravih sredove~nih stanovnika Srbije je bila glavni cilj na{e studije. Dodatno, analizirali smo potencijalnu povezanost gore- navedenih At sa serumskim proteinima i lipidima/lipopro- teinima. Metode: 40 zdravih ispitanika je bilo uklju~eno u na{u studiju. Gojaznost (BMI ≥ 30 kg/m2) je uo~ena kod 8/40 (20%) osoba. Titri analiziranih antitela su utvr|ivani ELISA testom. Rezultati: Prevalentnost IgG i IgM ab2gpIAt je bila 5% i 12.5%, redom, dok je prevalentnost IgM aCL bila 10%. Nivoi IgG ab2gpI At su se zna~ajno razlikovali izme|u ispi- tanika sa i bez hipertrigliceridemije (Mann-Whitney, P = 0.014). Zna~ajne razlike u hsCRP koncentracijama uo~ene su izme|u osoba sa povi{enim nivoima IgM aCL At i onih sa referentim vrednostima (Mann-Whitney, P = 0,028). Zaklju~ak: Dislipidemija i BMI ≥30 su bili povezani sa aPL At uprkos njihovoj niskoj prevalentnosti, i zato korekcija BMI i lipidnog statusa bi bila korisna u redukciji ili elimi- naciji predispoziraju}ih faktora koji mogu da izazovu trom- boti~ki doga|aj kod ina~e zdravih sredove~nih ispitanika. Obimnije nacionalne studije su neophodne da bi potvrdile na{e nalaze.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data, Antifosfolipidna antitela u zdravih srpskih osoba srednjih godina - preliminarni podaci",
volume = "41",
number = "4",
pages = "506-512",
doi = "10.5937/JOMB0-35642"
}

Bećarević, M., Jovičić, S., Ignjatović, S.,& Mirković, D.. (2022). Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 41(4), 506-512.
https://doi.org/10.5937/JOMB0-35642

Bećarević M, Jovičić S, Ignjatović S, Mirković D. Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data. in Journal of Medical Biochemistry. 2022;41(4):506-512.
doi:10.5937/JOMB0-35642 .

Bećarević, Mirjana, Jovičić, Snežana, Ignjatović, Svetlana, Mirković, Duško, "Antiphospholipid antibodies in healthy serbian middle-aged subjects: preliminary data" in Journal of Medical Biochemistry, 41, no. 4 (2022):506-512,
https://doi.org/10.5937/JOMB0-35642 . .

TY  - JOUR
AU  - Bećarević, Mirjana
AU  - Sarić-Matutinović, Marija
AU  - Žarković, Miloš
AU  - Nedeljković-Beleslin, Biljana
AU  - Ćirić, Jasmina
AU  - Ignjatović, Svetlana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3909
AB  - Purpose: Graves’ orbitopathy (GO) is an inflammatory autoimmune disorder of the orbit and while the antiphospholipid antibodies (aPL) Abs were associated with the markers of inflammation in the antiphospholipid syndrome (APS), there is no literature that investigate the presence of aPL Abs in GO. We analyzed the prevalence of aPL Abs and the differences between aPL (+) and aPL (−) subgroups of GO patients. Methods: Study included consecutive patients with GO (66 with Graves’ (GD), 10 with Hashimoto (HD), and 8 were euthyroid). Anticardiolipin (aCL) and anti-beta 2glycoprotein I (aβ2gpI) Abs were measured by ELISA. Results: aPL Abs were present in 9/84 (10.71%) patients. The IgM aβ2gpI Abs were present in 8/66 and in 1/10 patients with GD and HD. The IgG aCL Abs were present in one GD patient, and IgM aCL were present in 3/66 GD and in 1/10 patients with HD. In GD group, anti-Tg Abs were in positive correlation with aβ2gpI IgG (p = 0.000) and with anti-TPO Abs (p = 0.016). In HD group, anti-Tg Abs were in positive correlation with IgM aCL (p = 0.042), while anti-TPO Abs were in positive correlation with aβ2gpI IgM (p = 0.014). Conclusion: This study is the first report of the aPL Abs presence in GO patients. The anti-thyroid Abs were linked to aPL suggesting that their presence is not the sole consequence of hyperstimulation of autoreactive B-lymphocytes. Larger studies are necessary to confirm potential cause-effect relations. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PB  - Springer
T2  - Endocrine
T1  - Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data
DO  - 10.1007/s12020-021-02769-z
ER  - 

@article{
author = "Bećarević, Mirjana and Sarić-Matutinović, Marija and Žarković, Miloš and Nedeljković-Beleslin, Biljana and Ćirić, Jasmina and Ignjatović, Svetlana",
year = "2021",
abstract = "Purpose: Graves’ orbitopathy (GO) is an inflammatory autoimmune disorder of the orbit and while the antiphospholipid antibodies (aPL) Abs were associated with the markers of inflammation in the antiphospholipid syndrome (APS), there is no literature that investigate the presence of aPL Abs in GO. We analyzed the prevalence of aPL Abs and the differences between aPL (+) and aPL (−) subgroups of GO patients. Methods: Study included consecutive patients with GO (66 with Graves’ (GD), 10 with Hashimoto (HD), and 8 were euthyroid). Anticardiolipin (aCL) and anti-beta 2glycoprotein I (aβ2gpI) Abs were measured by ELISA. Results: aPL Abs were present in 9/84 (10.71%) patients. The IgM aβ2gpI Abs were present in 8/66 and in 1/10 patients with GD and HD. The IgG aCL Abs were present in one GD patient, and IgM aCL were present in 3/66 GD and in 1/10 patients with HD. In GD group, anti-Tg Abs were in positive correlation with aβ2gpI IgG (p = 0.000) and with anti-TPO Abs (p = 0.016). In HD group, anti-Tg Abs were in positive correlation with IgM aCL (p = 0.042), while anti-TPO Abs were in positive correlation with aβ2gpI IgM (p = 0.014). Conclusion: This study is the first report of the aPL Abs presence in GO patients. The anti-thyroid Abs were linked to aPL suggesting that their presence is not the sole consequence of hyperstimulation of autoreactive B-lymphocytes. Larger studies are necessary to confirm potential cause-effect relations. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
publisher = "Springer",
journal = "Endocrine",
title = "Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data",
doi = "10.1007/s12020-021-02769-z"
}

Bećarević, M., Sarić-Matutinović, M., Žarković, M., Nedeljković-Beleslin, B., Ćirić, J.,& Ignjatović, S.. (2021). Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data. in Endocrine
Springer..
https://doi.org/10.1007/s12020-021-02769-z

Bećarević M, Sarić-Matutinović M, Žarković M, Nedeljković-Beleslin B, Ćirić J, Ignjatović S. Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data. in Endocrine. 2021;.
doi:10.1007/s12020-021-02769-z .

Bećarević, Mirjana, Sarić-Matutinović, Marija, Žarković, Miloš, Nedeljković-Beleslin, Biljana, Ćirić, Jasmina, Ignjatović, Svetlana, "Antiphospholipid antibodies in patients with Graves’orbitopathy:preliminary data" in Endocrine (2021),
https://doi.org/10.1007/s12020-021-02769-z . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Nikolić, B.S
AU  - Ignjatović, Svetlana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3287
AB  - The antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of the IgG and/or IgM isotypes of the antiphospholipid antibodies, thrombosis and/or recurrent pregnancy losses. Various markers of inflammation are associated with clinical and/or laboratory features of APS. Adiponectin (Ad) is a member of the adipocytokines that exert its roles by binding to its receptors (AdR). Peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonists induced Ad production. The aged Pparg null-mice represented the first animal model that spontaneously develops APS and this model emphasized the importance of PPAR-gamma signaling in the development of APS. Recombinant Ad (rAd) application was beneficial for the improvement of glucose, insulin and lipid levels in mice. Orally active AdR agonist exerted similar effects to Ad in mice. Due to the re-occurrence of thrombotic episodes in APS patients (despite life-long anticoagulation), administration of PPAR-gamma agonists, rAd, or AdR agonists should be further tested in experimental models of APS, which eventually, will provide more data for novel therapeutic strategies that will ameliorate clinical manifestations of the APS.
PB  - Springer Verlag
T2  - Rheumatology International
T1  - Adiponectin: a therapeutic target in the antiphospholipid syndrome?
DO  - 10.1007/s00296-019-04349-x
ER  - 

@article{
author = "Becarević, Mirjana and Nikolić, B.S and Ignjatović, Svetlana",
year = "2019",
abstract = "The antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of the IgG and/or IgM isotypes of the antiphospholipid antibodies, thrombosis and/or recurrent pregnancy losses. Various markers of inflammation are associated with clinical and/or laboratory features of APS. Adiponectin (Ad) is a member of the adipocytokines that exert its roles by binding to its receptors (AdR). Peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonists induced Ad production. The aged Pparg null-mice represented the first animal model that spontaneously develops APS and this model emphasized the importance of PPAR-gamma signaling in the development of APS. Recombinant Ad (rAd) application was beneficial for the improvement of glucose, insulin and lipid levels in mice. Orally active AdR agonist exerted similar effects to Ad in mice. Due to the re-occurrence of thrombotic episodes in APS patients (despite life-long anticoagulation), administration of PPAR-gamma agonists, rAd, or AdR agonists should be further tested in experimental models of APS, which eventually, will provide more data for novel therapeutic strategies that will ameliorate clinical manifestations of the APS.",
publisher = "Springer Verlag",
journal = "Rheumatology International",
title = "Adiponectin: a therapeutic target in the antiphospholipid syndrome?",
doi = "10.1007/s00296-019-04349-x"
}

Becarević, M., Nikolić, B.S,& Ignjatović, S.. (2019). Adiponectin: a therapeutic target in the antiphospholipid syndrome?. in Rheumatology International
Springer Verlag..
https://doi.org/10.1007/s00296-019-04349-x

Becarević M, Nikolić B, Ignjatović S. Adiponectin: a therapeutic target in the antiphospholipid syndrome?. in Rheumatology International. 2019;.
doi:10.1007/s00296-019-04349-x .

Becarević, Mirjana, Nikolić, B.S, Ignjatović, Svetlana, "Adiponectin: a therapeutic target in the antiphospholipid syndrome?" in Rheumatology International (2019),
https://doi.org/10.1007/s00296-019-04349-x . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Sarić, Marija
AU  - Stojanović, Ljudmila
AU  - Mirković, Duško
AU  - Dopsaj, Violeta
AU  - Ignjatović, Svetlana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3063
AB  - Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome
VL  - 37
IS  - 12
SP  - 3359
EP  - 3364
DO  - 10.1007/s10067-018-4170-7
ER  - 

@article{
author = "Becarević, Mirjana and Sarić, Marija and Stojanović, Ljudmila and Mirković, Duško and Dopsaj, Violeta and Ignjatović, Svetlana",
year = "2018",
abstract = "Vascular antiphospholipid syndrome (VAPS) and obstetric (OAPS) are different entities because some patients only develop thrombosis (without recurrent pregnancy losses) and vice versa. Only two articles have reported that low 25-hydroxy-cholecalciferol (vitamin D3, VD3) levels were not correlated with the presence of conventional antiphospholipid antibodies (aPL Abs: anticardiolipin (aCL), anti-beta2glycoprotein I (a2gpI), and lupus anticoagulant (LA)), but no article analyzed the association of VD3 and anti-annexin A5 (aanxA5) Abs. The aim of our study was to investigate the association between VD3, multiple positivity of conventional aPL and aanxA5 Abs levels only in female OAPS vs. VAPS. Our study included 62 consecutive female PAPS patients. Concentrations of Abs were measured by ELISA, while VD3 levels were determined by immunochemiluminescence. Only 10/62 (16.13%) had sufficient (30ng/ml) VD3 levels, while 48/62 (77.42%) and 4/62 (6.45%) had insufficiency and VD3 deficiency, respectively. Statistically significant VD3 deficiency was noticed in VAPS (vs. OAPS, P=0.013). A negative correlation between VD3 levels and the age of patients was noticed (r=-0.493, P=0.032) only in VAPS subgroup. Multiple positivity of aPL and aanxA5 Abs was not associated with VD3 deficiency. Newly emerging aPL Abs, such as aanxA5 Abs, or their combinations with classical aPL Abs are not associated with VD3 deficiency in neither OAPS nor VAPS patients. Due to its immunomodulatory roles in B-Ly homeostasis, supplementation with VD3 should be considered in APS, at least in subgroup with severe form of the disease, i.e., VAPS.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome",
volume = "37",
number = "12",
pages = "3359-3364",
doi = "10.1007/s10067-018-4170-7"
}

Becarević, M., Sarić, M., Stojanović, L., Mirković, D., Dopsaj, V.,& Ignjatović, S.. (2018). Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 37(12), 3359-3364.
https://doi.org/10.1007/s10067-018-4170-7

Becarević M, Sarić M, Stojanović L, Mirković D, Dopsaj V, Ignjatović S. Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome. in Clinical Rheumatology. 2018;37(12):3359-3364.
doi:10.1007/s10067-018-4170-7 .

Becarević, Mirjana, Sarić, Marija, Stojanović, Ljudmila, Mirković, Duško, Dopsaj, Violeta, Ignjatović, Svetlana, "Anti-annexin A5 antibodies and 25-hydroxy-cholecalciferol in female patients with primary antiphospholipid syndrome" in Clinical Rheumatology, 37, no. 12 (2018):3359-3364,
https://doi.org/10.1007/s10067-018-4170-7 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Cabarkapa, Velibor
AU  - Derić, Mirjana
AU  - Ignjatović, Svetlana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3006
AB  - Objective: Antiphospholipid antibodies (aPL Abs) represented an independent factor that was associated with the occurrence and/or progression of nephropathy in patients with antiphospholipid syndrome, but their role in diabetic nephropathy is not elucidated. Therefore, we evaluated the association of aPL Abs with the renal impairment parameters in patients with diabetic nephropathy. Methods: Concentrations of analyzed antibodies were measured by enzyme-linked immunosorbent assay. Results: Cystatin C and anticardiolipin (aCL) antibodies of the immunoglobulin (Ig) G (r = .349, P = .004) and the IgM isotype (r = .316, P = .009) were in positive correlation. The IgG isotype of the aCL Abs was in positive correlation with creatinine (r = .252, P = .038), urea (r = .241, P = .048), and uric acid (r = .271, P = .025). The concentrations of the IgG isotype of the aCL Abs were significantly different between subgroups of patients with diabetic polyneuropathy and patients without this clinical finding (Mann-Whitney, P = .033). Conclusion: This is the first report on positive correlation between aCL Abs and renal impairment parameters. Larger studies are necessary for elucidation whether this association is involved in further progression of the disease.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Clinical and Applied Thrombosis-Hemostasis
T1  - Antiphospholipid Antibodies and Renal Impairment Parameters in Diabetic Nephropathy: Preliminary Data
VL  - 23
IS  - 4
SP  - 379
EP  - 383
DO  - 10.1177/1076029616642512
ER  - 

@article{
author = "Becarević, Mirjana and Cabarkapa, Velibor and Derić, Mirjana and Ignjatović, Svetlana",
year = "2017",
abstract = "Objective: Antiphospholipid antibodies (aPL Abs) represented an independent factor that was associated with the occurrence and/or progression of nephropathy in patients with antiphospholipid syndrome, but their role in diabetic nephropathy is not elucidated. Therefore, we evaluated the association of aPL Abs with the renal impairment parameters in patients with diabetic nephropathy. Methods: Concentrations of analyzed antibodies were measured by enzyme-linked immunosorbent assay. Results: Cystatin C and anticardiolipin (aCL) antibodies of the immunoglobulin (Ig) G (r = .349, P = .004) and the IgM isotype (r = .316, P = .009) were in positive correlation. The IgG isotype of the aCL Abs was in positive correlation with creatinine (r = .252, P = .038), urea (r = .241, P = .048), and uric acid (r = .271, P = .025). The concentrations of the IgG isotype of the aCL Abs were significantly different between subgroups of patients with diabetic polyneuropathy and patients without this clinical finding (Mann-Whitney, P = .033). Conclusion: This is the first report on positive correlation between aCL Abs and renal impairment parameters. Larger studies are necessary for elucidation whether this association is involved in further progression of the disease.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Clinical and Applied Thrombosis-Hemostasis",
title = "Antiphospholipid Antibodies and Renal Impairment Parameters in Diabetic Nephropathy: Preliminary Data",
volume = "23",
number = "4",
pages = "379-383",
doi = "10.1177/1076029616642512"
}

Becarević, M., Cabarkapa, V., Derić, M.,& Ignjatović, S.. (2017). Antiphospholipid Antibodies and Renal Impairment Parameters in Diabetic Nephropathy: Preliminary Data. in Clinical and Applied Thrombosis-Hemostasis
Sage Publications Inc, Thousand Oaks., 23(4), 379-383.
https://doi.org/10.1177/1076029616642512

Becarević M, Cabarkapa V, Derić M, Ignjatović S. Antiphospholipid Antibodies and Renal Impairment Parameters in Diabetic Nephropathy: Preliminary Data. in Clinical and Applied Thrombosis-Hemostasis. 2017;23(4):379-383.
doi:10.1177/1076029616642512 .

Becarević, Mirjana, Cabarkapa, Velibor, Derić, Mirjana, Ignjatović, Svetlana, "Antiphospholipid Antibodies and Renal Impairment Parameters in Diabetic Nephropathy: Preliminary Data" in Clinical and Applied Thrombosis-Hemostasis, 23, no. 4 (2017):379-383,
https://doi.org/10.1177/1076029616642512 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Ignjatović, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2733
AB  - The latest classification criteria for the diagnosis of the antiphospholipid syndrome (APS, an autoimmune disease characterized by thromboses, miscarriages and presence of antiphospholipid antibodies (Abs)) emphasized that thrombotic manifestations of APS should be without any signs of an inflammatory process. However, atherosclerosis (a chronic inflammatory response to the accumulation of lipoproteins in the walls of arteries) and APS are characterized by some similar features. We evaluated whether proinflammatory proteins were associated with the features of the primary APS (PAPS). PAPS patients without obstetric complications and with impaired lipid profile were included in the study. Antiphospholipid antibodies, TNF-alpha, and apo(a) were determined by ELISA. Complement components and hsCRP were measured by immunonephelometry. Decreased C3c was observed in female patients with increased titers of IgG anti-beta 2gpI (chi(2) = 3.939, P = 0.047) and in male patients with increased IgM anticardiolipin Abs (chi(2) = 4.286, P = 0.038). Pulmonary emboli were associated with interleukin (IL)-6 in male (chi(2) = 6.519, P = 0.011) and in female (chi(2) = 10.405, P = 0.001) patients. Cerebrovascular insults were associated with LDL-cholesterol (P = 0.05, 95 % CI: 1.003 - 12.739) in female and with apo(a) (P = 0.016, 95 % CI: 0.000-0.003) in male patients. Older female patients had increased LDL-cholesterol levels and frequency of myocardial infarctions. Proinflammatory proteins were associated with features of primary APS. No real gender differences in regard to proinflammatory protein levels were observed. Premenopausal state of female PAPS patients confers lower cardiovascular risk.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Proinflammatory proteins in female and male patients with primary antiphospholipid syndrome: preliminary data
VL  - 35
IS  - 10
SP  - 2477
EP  - 2483
DO  - 10.1007/s10067-016-3345-3
ER  - 

@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana",
year = "2016",
abstract = "The latest classification criteria for the diagnosis of the antiphospholipid syndrome (APS, an autoimmune disease characterized by thromboses, miscarriages and presence of antiphospholipid antibodies (Abs)) emphasized that thrombotic manifestations of APS should be without any signs of an inflammatory process. However, atherosclerosis (a chronic inflammatory response to the accumulation of lipoproteins in the walls of arteries) and APS are characterized by some similar features. We evaluated whether proinflammatory proteins were associated with the features of the primary APS (PAPS). PAPS patients without obstetric complications and with impaired lipid profile were included in the study. Antiphospholipid antibodies, TNF-alpha, and apo(a) were determined by ELISA. Complement components and hsCRP were measured by immunonephelometry. Decreased C3c was observed in female patients with increased titers of IgG anti-beta 2gpI (chi(2) = 3.939, P = 0.047) and in male patients with increased IgM anticardiolipin Abs (chi(2) = 4.286, P = 0.038). Pulmonary emboli were associated with interleukin (IL)-6 in male (chi(2) = 6.519, P = 0.011) and in female (chi(2) = 10.405, P = 0.001) patients. Cerebrovascular insults were associated with LDL-cholesterol (P = 0.05, 95 % CI: 1.003 - 12.739) in female and with apo(a) (P = 0.016, 95 % CI: 0.000-0.003) in male patients. Older female patients had increased LDL-cholesterol levels and frequency of myocardial infarctions. Proinflammatory proteins were associated with features of primary APS. No real gender differences in regard to proinflammatory protein levels were observed. Premenopausal state of female PAPS patients confers lower cardiovascular risk.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Proinflammatory proteins in female and male patients with primary antiphospholipid syndrome: preliminary data",
volume = "35",
number = "10",
pages = "2477-2483",
doi = "10.1007/s10067-016-3345-3"
}

Becarević, M.,& Ignjatović, S.. (2016). Proinflammatory proteins in female and male patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology
Springer London Ltd, London., 35(10), 2477-2483.
https://doi.org/10.1007/s10067-016-3345-3

Becarević M, Ignjatović S. Proinflammatory proteins in female and male patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology. 2016;35(10):2477-2483.
doi:10.1007/s10067-016-3345-3 .

Becarević, Mirjana, Ignjatović, Svetlana, "Proinflammatory proteins in female and male patients with primary antiphospholipid syndrome: preliminary data" in Clinical Rheumatology, 35, no. 10 (2016):2477-2483,
https://doi.org/10.1007/s10067-016-3345-3 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Mirković, Duško
AU  - Ignjatović, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2629
AB  - Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data
VL  - 35
IS  - 12
SP  - 2947
EP  - 2954
DO  - 10.1007/s10067-016-3438-z
ER  - 

@article{
author = "Becarević, Mirjana and Mirković, Duško and Ignjatović, Svetlana",
year = "2016",
abstract = "Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data",
volume = "35",
number = "12",
pages = "2947-2954",
doi = "10.1007/s10067-016-3438-z"
}

Becarević, M., Mirković, D.,& Ignjatović, S.. (2016). Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology
Springer London Ltd, London., 35(12), 2947-2954.
https://doi.org/10.1007/s10067-016-3438-z

Becarević M, Mirković D, Ignjatović S. Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology. 2016;35(12):2947-2954.
doi:10.1007/s10067-016-3438-z .

Becarević, Mirjana, Mirković, Duško, Ignjatović, Svetlana, "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data" in Clinical Rheumatology, 35, no. 12 (2016):2947-2954,
https://doi.org/10.1007/s10067-016-3438-z . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Stojanović, Ljudmila
AU  - Ignjatović, Svetlana
AU  - Dopsaj, Violeta
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2590
AB  - We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome
VL  - 35
IS  - 5
SP  - 1361
EP  - 1365
DO  - 10.1007/s10067-016-3230-0
ER  - 

@article{
author = "Becarević, Mirjana and Stojanović, Ljudmila and Ignjatović, Svetlana and Dopsaj, Violeta",
year = "2016",
abstract = "We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome",
volume = "35",
number = "5",
pages = "1361-1365",
doi = "10.1007/s10067-016-3230-0"
}

Becarević, M., Stojanović, L., Ignjatović, S.,& Dopsaj, V.. (2016). The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 35(5), 1361-1365.
https://doi.org/10.1007/s10067-016-3230-0

Becarević M, Stojanović L, Ignjatović S, Dopsaj V. The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology. 2016;35(5):1361-1365.
doi:10.1007/s10067-016-3230-0 .

Becarević, Mirjana, Stojanović, Ljudmila, Ignjatović, Svetlana, Dopsaj, Violeta, "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome" in Clinical Rheumatology, 35, no. 5 (2016):1361-1365,
https://doi.org/10.1007/s10067-016-3230-0 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1934
AB  - It has been proposed that apoptosis is one of the mechanisms involved in the generation of antiphospholipid antibodies. The presence of antiphospholipid antibodies is the main laboratory criterion for a definite diagnosis of the antiphospholipid syndrome. Annexinopathies are disorders characterized by deregulation of annexins expression levels and function. Annexin A5 has been used as an agent for molecular imaging techniques (visualization of phosphatidylserine-expressing apoptotic cells) in vitro and in vivo in animal models and in patients (injection of human recombinant anxA5 into the patient's circulation). Although the determination of titers of anti-annexin A5 antibodies is not mandatory for the diagnosis of the antiphospholipid syndrome, it was reported that patients with primary antiphospholipid syndrome with a history of recurrent abortions had elevated titers of anti-annexin A5 antibodies, while the presence of thromboses was not associated with elevated levels of these antibodies.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome
VL  - 32
IS  - 2
SP  - 89
EP  - 95
DO  - 10.2478/jomb-2013-0014
ER  - 

@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2013",
abstract = "It has been proposed that apoptosis is one of the mechanisms involved in the generation of antiphospholipid antibodies. The presence of antiphospholipid antibodies is the main laboratory criterion for a definite diagnosis of the antiphospholipid syndrome. Annexinopathies are disorders characterized by deregulation of annexins expression levels and function. Annexin A5 has been used as an agent for molecular imaging techniques (visualization of phosphatidylserine-expressing apoptotic cells) in vitro and in vivo in animal models and in patients (injection of human recombinant anxA5 into the patient's circulation). Although the determination of titers of anti-annexin A5 antibodies is not mandatory for the diagnosis of the antiphospholipid syndrome, it was reported that patients with primary antiphospholipid syndrome with a history of recurrent abortions had elevated titers of anti-annexin A5 antibodies, while the presence of thromboses was not associated with elevated levels of these antibodies.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome",
volume = "32",
number = "2",
pages = "89-95",
doi = "10.2478/jomb-2013-0014"
}

Becarević, M., Ignjatović, S.,& Majkić-Singh, N.. (2013). Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(2), 89-95.
https://doi.org/10.2478/jomb-2013-0014

Becarević M, Ignjatović S, Majkić-Singh N. Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome. in Journal of Medical Biochemistry. 2013;32(2):89-95.
doi:10.2478/jomb-2013-0014 .

Becarević, Mirjana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Apoptosis, annexin a5 and anti-Annexin a5 antibodies in the antiphospholipid syndrome" in Journal of Medical Biochemistry, 32, no. 2 (2013):89-95,
https://doi.org/10.2478/jomb-2013-0014 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1756
AB  - The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus
VL  - 31
IS  - 3
SP  - 199
EP  - 204
DO  - 10.2478/v10011-012-0009-y
ER  - 

@article{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Singh, Sandra and Majkić-Singh, Nada",
year = "2012",
abstract = "The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus",
volume = "31",
number = "3",
pages = "199-204",
doi = "10.2478/v10011-012-0009-y"
}

Becarević, M., Seferović, J., Ignjatović, S., Singh, S.,& Majkić-Singh, N.. (2012). Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(3), 199-204.
https://doi.org/10.2478/v10011-012-0009-y

Becarević M, Seferović J, Ignjatović S, Singh S, Majkić-Singh N. Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry. 2012;31(3):199-204.
doi:10.2478/v10011-012-0009-y .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Singh, Sandra, Majkić-Singh, Nada, "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus" in Journal of Medical Biochemistry, 31, no. 3 (2012):199-204,
https://doi.org/10.2478/v10011-012-0009-y . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1632
AB  - Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies
VL  - 58
IS  - 9-10
SP  - 1079
EP  - 1084
DO  - 10.7754/Clin.Lab.2012.110918
ER  - 

@article{
author = "Becarević, Mirjana and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2012",
abstract = "Background: To investigate the association between clinical and serological features of patients with primary antiphospholipid syndrome (PAPS) and TNF-alpha, interleukin 6 (IL-6), and soluble IL-2 receptor (sIL-2R). Methods: ELISA was used for measurement of antibodies (Abs) and TNF-alpha, while IL-6 and sIL-2R were measured by chemiluminescence. Results: PAPS patients with pulmonary emboli showed positive correlation between IgM isotype of anti-annexin A5 antibodies and TNF-alpha (r = 0.894, p = 0.041) and IgM class of anticardiolipin antibodies and sIL-2R (r = 0.900, p = 0.037). In PAPS with cerebrovascular insults, positive correlation was noticed between TNF-alpha and IgG isotype of anticardiolipin (r = 0.624, p = 0.040) and anti-annexinA5 Abs (r = 0.768, p = 0.006). Conclusions: Isotype analysis of antiphospholipid and anti-annexin A5 Abs and investigation of their association with TNF-alpha is important for differentiation of PAPS patients that are prone to further deterioration of arterial and venous thromboses. (Clin. Lab. 2012;58:1079-1084. DOI: 10.7754/Clin.Lab.2012.110918)",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies",
volume = "58",
number = "9-10",
pages = "1079-1084",
doi = "10.7754/Clin.Lab.2012.110918"
}

Becarević, M., Ignjatović, S.,& Majkić-Singh, N.. (2012). Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 58(9-10), 1079-1084.
https://doi.org/10.7754/Clin.Lab.2012.110918

Becarević M, Ignjatović S, Majkić-Singh N. Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies. in Clinical Laboratory. 2012;58(9-10):1079-1084.
doi:10.7754/Clin.Lab.2012.110918 .

Becarević, Mirjana, Ignjatović, Svetlana, Majkić-Singh, Nada, "Deterioration of Thromboses in Primary Antiphospholipid Syndrome: TNF-alpha and Anti-Annexin A5 Antibodies" in Clinical Laboratory, 58, no. 9-10 (2012):1079-1084,
https://doi.org/10.7754/Clin.Lab.2012.110918 . .

TY  - CONF
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1495
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis Supplements
T1  - Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus
VL  - 12
IS  - 1
SP  - 91
EP  - 91
DO  - 10.1016/S1567-5688(11)70427-5
ER  - 

@conference{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Majkić-Singh, Nada",
year = "2011",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis Supplements",
title = "Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus",
volume = "12",
number = "1",
pages = "91-91",
doi = "10.1016/S1567-5688(11)70427-5"
}

Becarević, M., Seferović, J., Ignjatović, S.,& Majkić-Singh, N.. (2011). Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus. in Atherosclerosis Supplements
Elsevier Ireland Ltd, Clare., 12(1), 91-91.
https://doi.org/10.1016/S1567-5688(11)70427-5

Becarević M, Seferović J, Ignjatović S, Majkić-Singh N. Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus. in Atherosclerosis Supplements. 2011;12(1):91-91.
doi:10.1016/S1567-5688(11)70427-5 .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Majkić-Singh, Nada, "Association of tnf-alpha and anticardiolipin antibodies in type ii diabetes mellitus" in Atherosclerosis Supplements, 12, no. 1 (2011):91-91,
https://doi.org/10.1016/S1567-5688(11)70427-5 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1552
AB  - Objective: In vitro, stimulation of monocytes with antiphospholipid (aPL) antibodies resulted in increased secretion of TNF-alpha, but association of aPL with features of diabetes mellitus is not clarified yet. Therefore, we investigated the distribution of anticardiolipin (aCL), anti-beta 2gpI (a beta 2gpI), anti-annexin A5 (aannxA5), and anti-oxLDL (aoxLDL) antibodies, and TNF-alpha in well-formed group of 78 patients with type II diabetes mellitus without vascular complications. Methods: Investigated antibodies and TNF-alpha concentrations were measured by ELISA. Results: Antiphospholipid antibodies were in positive correlation with TNF-alpha concentrations: aCL IgG (r = 0.303, p = 0.007), aCL IgM (r = 0.386, p = 0.000), a beta 2gpI IgG (r = 0.499, p = 0.000), a beta 2gpI IgM (r = 0.462, p = 0.000), aanxA5 IgG (r = 0.479, p = 0.000), aanxA5 IgM (r = 0.641, p = 0.000), aoxLDL (IgG + IgM, r = 0.279, p = 0.000). Anticardiolipin-positive and aCL-negative subgroups differed in TNF-alpha concentrations (Mann-Whitney, p = 0.032). Significantly elevated LDL concentrations were noticed in aCL-positive patients with disease duration 10-15 years (chi(2) = 15.000, p = 0.000) and apoB concentrations were elevated in aoxLDL-positive patients with disease duration 7-10 years (chi(2) = 3.938, p = 0.047). Conclusion: Significant association of antiphospholipid antibodies and TNF-alpha might be a marker of severe atherogenic profile (suggested by increased levels of lipids in aPL-positive subgroups) and should be used for the stratification of patients with an increased risk for future deterioration of the disease.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Cytokine
T1  - Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications
VL  - 55
IS  - 2
SP  - 301
EP  - 306
DO  - 10.1016/j.cyto.2011.05.001
ER  - 

@article{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Singh, Sandra and Majkić-Singh, Nada",
year = "2011",
abstract = "Objective: In vitro, stimulation of monocytes with antiphospholipid (aPL) antibodies resulted in increased secretion of TNF-alpha, but association of aPL with features of diabetes mellitus is not clarified yet. Therefore, we investigated the distribution of anticardiolipin (aCL), anti-beta 2gpI (a beta 2gpI), anti-annexin A5 (aannxA5), and anti-oxLDL (aoxLDL) antibodies, and TNF-alpha in well-formed group of 78 patients with type II diabetes mellitus without vascular complications. Methods: Investigated antibodies and TNF-alpha concentrations were measured by ELISA. Results: Antiphospholipid antibodies were in positive correlation with TNF-alpha concentrations: aCL IgG (r = 0.303, p = 0.007), aCL IgM (r = 0.386, p = 0.000), a beta 2gpI IgG (r = 0.499, p = 0.000), a beta 2gpI IgM (r = 0.462, p = 0.000), aanxA5 IgG (r = 0.479, p = 0.000), aanxA5 IgM (r = 0.641, p = 0.000), aoxLDL (IgG + IgM, r = 0.279, p = 0.000). Anticardiolipin-positive and aCL-negative subgroups differed in TNF-alpha concentrations (Mann-Whitney, p = 0.032). Significantly elevated LDL concentrations were noticed in aCL-positive patients with disease duration 10-15 years (chi(2) = 15.000, p = 0.000) and apoB concentrations were elevated in aoxLDL-positive patients with disease duration 7-10 years (chi(2) = 3.938, p = 0.047). Conclusion: Significant association of antiphospholipid antibodies and TNF-alpha might be a marker of severe atherogenic profile (suggested by increased levels of lipids in aPL-positive subgroups) and should be used for the stratification of patients with an increased risk for future deterioration of the disease.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Cytokine",
title = "Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications",
volume = "55",
number = "2",
pages = "301-306",
doi = "10.1016/j.cyto.2011.05.001"
}

Becarević, M., Seferović, J., Ignjatović, S., Singh, S.,& Majkić-Singh, N.. (2011). Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications. in Cytokine
Academic Press Ltd- Elsevier Science Ltd, London., 55(2), 301-306.
https://doi.org/10.1016/j.cyto.2011.05.001

Becarević M, Seferović J, Ignjatović S, Singh S, Majkić-Singh N. Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications. in Cytokine. 2011;55(2):301-306.
doi:10.1016/j.cyto.2011.05.001 .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Singh, Sandra, Majkić-Singh, Nada, "Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications" in Cytokine, 55, no. 2 (2011):301-306,
https://doi.org/10.1016/j.cyto.2011.05.001 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Mirković, Duško
AU  - Majkić-Singh, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1161
AB  - The thrombotic tendency in anti phospholipid syndrome (APS) shares several pathways with atherosclerosis. Atherothrombosis (atherosclerosis superimposed with thromboses) is influenced by nonmodifiable and some modifiable risk factors (smoking, obesity, physical inactivity, alcohol abuse, hyperhomocysteinemia). Therefore, we investigated the association among clinical and serological features of patients with primary APS and potentially modifiable risk factors for the development of atherothrombosis. Also, we compared the analyzed parameters with those in control subjects. Homo cysteine concentrations were detected by HPLC (high performance liquid chromatography), while antiphospholipid antibodies were detected by ELISA. Smokers had elevated levels of homocysteine (chi(2) = 6.22, p  lt  0.05). Independently of patients' age, the association between increased levels of homocysteine and history of myocardial infarctions was found (chi(2) = 4.61, p  lt  0.05). Hyperhomocysteinemia and smoking are the most important modifiable risk factors for atherothrombosis in primary APS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Hyperhomocysteinemia and smoking in primary antiphospholipid syndrome
VL  - 28
IS  - 3
SP  - 172
EP  - 175
DO  - 10.2478/v10011-009-0019-6
ER  - 

@article{
author = "Becarević, Mirjana and Mirković, Duško and Majkić-Singh, Nada",
year = "2009",
abstract = "The thrombotic tendency in anti phospholipid syndrome (APS) shares several pathways with atherosclerosis. Atherothrombosis (atherosclerosis superimposed with thromboses) is influenced by nonmodifiable and some modifiable risk factors (smoking, obesity, physical inactivity, alcohol abuse, hyperhomocysteinemia). Therefore, we investigated the association among clinical and serological features of patients with primary APS and potentially modifiable risk factors for the development of atherothrombosis. Also, we compared the analyzed parameters with those in control subjects. Homo cysteine concentrations were detected by HPLC (high performance liquid chromatography), while antiphospholipid antibodies were detected by ELISA. Smokers had elevated levels of homocysteine (chi(2) = 6.22, p  lt  0.05). Independently of patients' age, the association between increased levels of homocysteine and history of myocardial infarctions was found (chi(2) = 4.61, p  lt  0.05). Hyperhomocysteinemia and smoking are the most important modifiable risk factors for atherothrombosis in primary APS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Hyperhomocysteinemia and smoking in primary antiphospholipid syndrome",
volume = "28",
number = "3",
pages = "172-175",
doi = "10.2478/v10011-009-0019-6"
}

Becarević, M., Mirković, D.,& Majkić-Singh, N.. (2009). Hyperhomocysteinemia and smoking in primary antiphospholipid syndrome. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 28(3), 172-175.
https://doi.org/10.2478/v10011-009-0019-6

Becarević M, Mirković D, Majkić-Singh N. Hyperhomocysteinemia and smoking in primary antiphospholipid syndrome. in Journal of Medical Biochemistry. 2009;28(3):172-175.
doi:10.2478/v10011-009-0019-6 .

Becarević, Mirjana, Mirković, Duško, Majkić-Singh, Nada, "Hyperhomocysteinemia and smoking in primary antiphospholipid syndrome" in Journal of Medical Biochemistry, 28, no. 3 (2009):172-175,
https://doi.org/10.2478/v10011-009-0019-6 . .