TY  - JOUR
AU  - Đelić, N
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Đelić, D
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/851
AB  - Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.
PB  - Elsevier Science BV, Amsterdam
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro
VL  - 604
IS  - 1-2
SP  - 1
EP  - 7
DO  - 10.1016/j.mrgentox.2005.11.013
ER  - 

@article{
author = "Đelić, N and Potparević, Biljana and Bajić, Vladan and Đelić, D",
year = "2006",
abstract = "Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro",
volume = "604",
number = "1-2",
pages = "1-7",
doi = "10.1016/j.mrgentox.2005.11.013"
}

Đelić, N., Potparević, B., Bajić, V.,& Đelić, D.. (2006). Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier Science BV, Amsterdam., 604(1-2), 1-7.
https://doi.org/10.1016/j.mrgentox.2005.11.013

Đelić N, Potparević B, Bajić V, Đelić D. Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2006;604(1-2):1-7.
doi:10.1016/j.mrgentox.2005.11.013 .

Đelić, N, Potparević, Biljana, Bajić, Vladan, Đelić, D, "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 604, no. 1-2 (2006):1-7,
https://doi.org/10.1016/j.mrgentox.2005.11.013 . .

TY  - JOUR
AU  - Đelić, N
AU  - Potparević, Biljana
AU  - Đelić, D
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/622
AB  - The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time.
PB  - Akademiai Kiado, Budapest
T2  - Acta Biologica Hungarica
T1  - Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication
VL  - 56
IS  - 3-4
SP  - 403
EP  - 406
DO  - 10.1556/ABiol.56.2005.3-4.22
ER  - 

@article{
author = "Đelić, N and Potparević, Biljana and Đelić, D",
year = "2005",
abstract = "The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time.",
publisher = "Akademiai Kiado, Budapest",
journal = "Acta Biologica Hungarica",
title = "Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication",
volume = "56",
number = "3-4",
pages = "403-406",
doi = "10.1556/ABiol.56.2005.3-4.22"
}

Đelić, N., Potparević, B.,& Đelić, D.. (2005). Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication. in Acta Biologica Hungarica
Akademiai Kiado, Budapest., 56(3-4), 403-406.
https://doi.org/10.1556/ABiol.56.2005.3-4.22

Đelić N, Potparević B, Đelić D. Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication. in Acta Biologica Hungarica. 2005;56(3-4):403-406.
doi:10.1556/ABiol.56.2005.3-4.22 .

Đelić, N, Potparević, Biljana, Đelić, D, "Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay - Short communication" in Acta Biologica Hungarica, 56, no. 3-4 (2005):403-406,
https://doi.org/10.1556/ABiol.56.2005.3-4.22 . .