@conference{
author = "Arsenijević, Jelena and Jovanović, Aleksandar and Jevtić, Stefan and Kovačević, Nada and Drobac, Milica",
year = "2018",
abstract = "Herba bosiljka se tradicionalno koristi kao karminativ, spazmolitik, blag sedativ i
laktagog. Etarsko ulje herbe bosiljka sadrži fenilpropanoid estragol, koji je kancerogen i
genotoksičan, a čija količina zavisi od hemotipa bosiljka. Količina estragola koja se
unosi putem biljnih lekovitih proizvoda, prema preporuci Evropske agencije za lekove
(EMA), ograničena je na 0,5 mg dnevno tokom 14 dana. Cilj rada bio je ispitivanje
hemijskog sastava etarskih ulja, sadržaja estragola u etarskim uljima i infuzima herbe
bosiljka, dostupne u Srbiji.
Ispitivani su komercijalni uzorci monokomponentnih čajeva i uzorci herbe
bosiljka gajenog u domaćinstvima u različitim delovima Srbije. Infuzi su pripremljeni
prelivanjem uzoraka ključalom vodom (2 g/150 ml) i ceđenjem nakon 15 min.
Izolovanje etarskog ulja iz herbe, kao i izolovanje estragola iz infuza izvršeno je
destilacijom vodenom parom u aparaturi po Klevendžeru. Analiza etarskih ulja i
destilata infuza izvršena je GC‐FID‐MS metodom. Sadržaj estragola u etarskim uljima i
infuzima određen je metodom eksternog standarda.
Sastav svih ispitivanih etarskih ulja odgovara evropskom hemotipu bosiljka,
kojeg karakteriše visok sadržaj linalola ili linalola i estragola. Sadržaj estragola u
ispitivanim etarskim uljima iznosio je 2,1‐565,4 mg/ml, a u infuzima 0,5‐11,3 μg/ml.
Primenom ispitivanih infuza prema preporuci Deutsche Arzneimittel‐Codex (DAC) (2
g/150 ml, tri puta dnevno), dnevni unos estragola iznosio bi 0,2‐5,1 mg.
Primenom infuza pojedinih uzoraka herbe bosiljka postoji mogućnost
prekoračenjа dozvoljenоg dnevnog unosa estragola. Dobijeni rezultati ukazuju na
značaj utvrđivanja sadržaja estragola u herbi bosiljka koja se koristi u obliku biljnih
lekovitih proizvoda., Basil herb is traditionally used as carminative, spasmolytic, mild sedative and
lactagogue. Basil essential oil contains phenylpropanoide estragole, which is
carcinogenic and genotoxic, and its amount depends on Basil chemotype. The amount
of estragole administered through herbal medicinal products, according to the
recommendation of the European Medicines Agency (EMA), is limited to 0.5 mg daily
during 14 days. The aim of this work was examination of chemical composition of
essential oils, as well as determination of estragole content in essential oils and
infusions of Basil herb available in Serbia.
We have analyzed commercial samples of monocomponent Basil herbal teas and
samples of Basil herb cultivated in different parts of Serbia. Infusions were prepared by
steeping samples in boiling water (2 g/150 ml) for 15 min and subsequent filtering.
Isolation of the herb essential oil, as well as isolation of estragole from infusions was
carried out by hydrodistillation using a Clevenger‐type apparatus. Analyses of essential
oils and infusion distillates were performed using GC‐FID‐MS. The content of estragole
in essential oils and infusions was determined using external standard method.
Chemical composition of all investigated essential oils corresponds to European
chemotype of Basil that is characterized by high content of linalool or linalool and
estragole. The content of estragole in essential oils ranged from 2.1 to 565.4 mg/ml,
whereas infusions contained 0.5‐11.3 μg/ml of this compound. If the examined
infusions are administered according to the recommendation of Deutsche Arzneimittel‐
Codex (DAC) (2 g/150 ml, three times daily), the daily intake of estragole would be 0.2‐
5.1 mg.
By administration of infusions of some Basil herb samples, there is a possibility
of exceeding the permitted daily intake of estragole. The obtained results indicate the
importance of determining the content of estragole in Basil herb used in the form of
herbal medicinal products.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L., The content of estragole in essential oil and infusion of basil herb, Ocimum basilicum L.",
volume = "68",
number = "3",
pages = "712-713",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5279"
}
