TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Skapin, Sreco D.
AU  - Bracko, Ines
AU  - Milenković, Marina
AU  - Petković, Jana
AU  - Filipić, Metka
AU  - Uskoković, Dragan P.
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1733
AB  - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (alpha, gamma, L-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV-VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer
T1  - Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
VL  - 53
IS  - 14
SP  - 2818
EP  - 2828
DO  - 10.1016/j.polymer.2012.04.057
ER  - 

@article{
author = "Stevanović, Magdalena and Skapin, Sreco D. and Bracko, Ines and Milenković, Marina and Petković, Jana and Filipić, Metka and Uskoković, Dragan P.",
year = "2012",
abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (alpha, gamma, L-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV-VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer",
title = "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential",
volume = "53",
number = "14",
pages = "2818-2828",
doi = "10.1016/j.polymer.2012.04.057"
}

Stevanović, M., Skapin, S. D., Bracko, I., Milenković, M., Petković, J., Filipić, M.,& Uskoković, D. P.. (2012). Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer
Elsevier Sci Ltd, Oxford., 53(14), 2818-2828.
https://doi.org/10.1016/j.polymer.2012.04.057

Stevanović M, Skapin SD, Bracko I, Milenković M, Petković J, Filipić M, Uskoković DP. Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer. 2012;53(14):2818-2828.
doi:10.1016/j.polymer.2012.04.057 .

Stevanović, Magdalena, Skapin, Sreco D., Bracko, Ines, Milenković, Marina, Petković, Jana, Filipić, Metka, Uskoković, Dragan P., "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential" in Polymer, 53, no. 14 (2012):2818-2828,
https://doi.org/10.1016/j.polymer.2012.04.057 . .