TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Topić, Aleksandra
AU  - Savković, Miljan
AU  - Milinković, Neda
AU  - Novaković, Ivana
AU  - Cujić, Danica
AU  - Simić-Ogrizović, Sanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3366
AB  - Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.
PB  - Hindawi Ltd, London
T2  - Disease Markers
T1  - Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease
DO  - 10.1155/2019/4864370
ER  - 

@article{
author = "Dopsaj, Violeta and Topić, Aleksandra and Savković, Miljan and Milinković, Neda and Novaković, Ivana and Cujić, Danica and Simić-Ogrizović, Sanja",
year = "2019",
abstract = "Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.",
publisher = "Hindawi Ltd, London",
journal = "Disease Markers",
title = "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease",
doi = "10.1155/2019/4864370"
}

Dopsaj, V., Topić, A., Savković, M., Milinković, N., Novaković, I., Cujić, D.,& Simić-Ogrizović, S.. (2019). Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers
Hindawi Ltd, London..
https://doi.org/10.1155/2019/4864370

Dopsaj V, Topić A, Savković M, Milinković N, Novaković I, Cujić D, Simić-Ogrizović S. Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers. 2019;.
doi:10.1155/2019/4864370 .

Dopsaj, Violeta, Topić, Aleksandra, Savković, Miljan, Milinković, Neda, Novaković, Ivana, Cujić, Danica, Simić-Ogrizović, Sanja, "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease" in Disease Markers (2019),
https://doi.org/10.1155/2019/4864370 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Kontić-Vucinić, Olivera
AU  - Novaković, Ivana
AU  - Ignjatović, Svetlana
AU  - Mijović, Marija
AU  - Sulović, Nenad
AU  - Vukolić, Dušan
AU  - Komnenić, Milica
AU  - Tadić, Jasmina
AU  - Cetković, Aleksandar
AU  - Belić, Aleksandra
AU  - Ljubić, Aleksandar
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2572
AB  - This is the first study in Serbia and the region of South-East Europe dedicated to clients' perception of outcome and efficiency of prenatal and reproductive genetic counseling. The primary aim of this study was to assess overall value and success of genetic counseling in prenatal and reproductive care with regard to perceived personal control of clients, reflecting also in a part patient comprehension, knowledge retention, and empowerment in decision-making. The standardized Perceived Personal Control questionnaire (PPC) was used for the assessment of 239 female participants. First, we performed a complete validation of the psychometric characteristics of the Serbian-language version of the PPC questionnaire. The validation of the questionnaire permits other researchers from Serbian-speaking regions of South-East Europe to use this standard instrument to assess the effectiveness of prenatal genetic counseling in their communities and analyze advantages and disadvantages of their counseling models. We also measured social and demographic characteristics of participants. Further, we analyzed effects of our team-based prenatal and reproductive genetic counseling model through (a) calculation of PPC scores at three different stages (before initial, after initial, and before second counseling session), and (b) by assessing participants' responses by indication for referral (advanced maternal age, abnormal biochemical screening, family history of hereditary disorders, maternal exposure to drugs, exposure to radiation, exposure to infective agents, infertility or recurrent abortions, and miscellaneous). The results indicate that participants' knowledge after initial counseling increased significantly and after that remained stable and sustainable. A satisfactory level of confidence among participants had been achieved, in that many felt an increased sense of control over their situation and emotional response to it. Indirectly, these results indicate the success of a team-based prenatal genetic counseling model, which has not been assessed in the literature to date.
PB  - Springer, New York
T2  - Journal of Genetic Counseling
T1  - Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire
VL  - 25
IS  - 1
SP  - 189
EP  - 197
DO  - 10.1007/s10897-015-9857-1
ER  - 

@article{
author = "Cuturilo, Goran and Kontić-Vucinić, Olivera and Novaković, Ivana and Ignjatović, Svetlana and Mijović, Marija and Sulović, Nenad and Vukolić, Dušan and Komnenić, Milica and Tadić, Jasmina and Cetković, Aleksandar and Belić, Aleksandra and Ljubić, Aleksandar",
year = "2016",
abstract = "This is the first study in Serbia and the region of South-East Europe dedicated to clients' perception of outcome and efficiency of prenatal and reproductive genetic counseling. The primary aim of this study was to assess overall value and success of genetic counseling in prenatal and reproductive care with regard to perceived personal control of clients, reflecting also in a part patient comprehension, knowledge retention, and empowerment in decision-making. The standardized Perceived Personal Control questionnaire (PPC) was used for the assessment of 239 female participants. First, we performed a complete validation of the psychometric characteristics of the Serbian-language version of the PPC questionnaire. The validation of the questionnaire permits other researchers from Serbian-speaking regions of South-East Europe to use this standard instrument to assess the effectiveness of prenatal genetic counseling in their communities and analyze advantages and disadvantages of their counseling models. We also measured social and demographic characteristics of participants. Further, we analyzed effects of our team-based prenatal and reproductive genetic counseling model through (a) calculation of PPC scores at three different stages (before initial, after initial, and before second counseling session), and (b) by assessing participants' responses by indication for referral (advanced maternal age, abnormal biochemical screening, family history of hereditary disorders, maternal exposure to drugs, exposure to radiation, exposure to infective agents, infertility or recurrent abortions, and miscellaneous). The results indicate that participants' knowledge after initial counseling increased significantly and after that remained stable and sustainable. A satisfactory level of confidence among participants had been achieved, in that many felt an increased sense of control over their situation and emotional response to it. Indirectly, these results indicate the success of a team-based prenatal genetic counseling model, which has not been assessed in the literature to date.",
publisher = "Springer, New York",
journal = "Journal of Genetic Counseling",
title = "Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire",
volume = "25",
number = "1",
pages = "189-197",
doi = "10.1007/s10897-015-9857-1"
}

Cuturilo, G., Kontić-Vucinić, O., Novaković, I., Ignjatović, S., Mijović, M., Sulović, N., Vukolić, D., Komnenić, M., Tadić, J., Cetković, A., Belić, A.,& Ljubić, A.. (2016). Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire. in Journal of Genetic Counseling
Springer, New York., 25(1), 189-197.
https://doi.org/10.1007/s10897-015-9857-1

Cuturilo G, Kontić-Vucinić O, Novaković I, Ignjatović S, Mijović M, Sulović N, Vukolić D, Komnenić M, Tadić J, Cetković A, Belić A, Ljubić A. Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire. in Journal of Genetic Counseling. 2016;25(1):189-197.
doi:10.1007/s10897-015-9857-1 .

Cuturilo, Goran, Kontić-Vucinić, Olivera, Novaković, Ivana, Ignjatović, Svetlana, Mijović, Marija, Sulović, Nenad, Vukolić, Dušan, Komnenić, Milica, Tadić, Jasmina, Cetković, Aleksandar, Belić, Aleksandra, Ljubić, Aleksandar, "Clients' Perception of Outcome of Team-Based Prenatal and Reproductive Genetic Counseling in Serbian Service Using the Perceived Personal Control (PPC) Questionnaire" in Journal of Genetic Counseling, 25, no. 1 (2016):189-197,
https://doi.org/10.1007/s10897-015-9857-1 . .

TY  - JOUR
AU  - Novaković, Ivana
AU  - Maksimović, Nela
AU  - Pavlović, Aleksandra
AU  - Žarković, Milena
AU  - Rovcanin, Branislav
AU  - Mirković, Duško
AU  - Pekmezović, Tatjana
AU  - Cvetković, Dragana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2148
AB  - Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Introduction to Molecular genetic diagnostics
VL  - 33
IS  - 1
SP  - 3
EP  - 7
DO  - 10.2478/jomb-2013-0039
ER  - 

@article{
author = "Novaković, Ivana and Maksimović, Nela and Pavlović, Aleksandra and Žarković, Milena and Rovcanin, Branislav and Mirković, Duško and Pekmezović, Tatjana and Cvetković, Dragana",
year = "2014",
abstract = "Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Introduction to Molecular genetic diagnostics",
volume = "33",
number = "1",
pages = "3-7",
doi = "10.2478/jomb-2013-0039"
}

Novaković, I., Maksimović, N., Pavlović, A., Žarković, M., Rovcanin, B., Mirković, D., Pekmezović, T.,& Cvetković, D.. (2014). Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 33(1), 3-7.
https://doi.org/10.2478/jomb-2013-0039

Novaković I, Maksimović N, Pavlović A, Žarković M, Rovcanin B, Mirković D, Pekmezović T, Cvetković D. Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry. 2014;33(1):3-7.
doi:10.2478/jomb-2013-0039 .

Novaković, Ivana, Maksimović, Nela, Pavlović, Aleksandra, Žarković, Milena, Rovcanin, Branislav, Mirković, Duško, Pekmezović, Tatjana, Cvetković, Dragana, "Introduction to Molecular genetic diagnostics" in Journal of Medical Biochemistry, 33, no. 1 (2014):3-7,
https://doi.org/10.2478/jomb-2013-0039 . .

TY  - JOUR
AU  - Todorović, Zoran
AU  - Džoljić, Eleonora
AU  - Novaković, Ivana
AU  - Mirković, Duško
AU  - Stojanović, Radan
AU  - Nesić, Zorica
AU  - Krajinović, Maja
AU  - Prostran, Milica
AU  - Kostić, Vladimir
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/666
AB  - Both methylenetetrahydrofolate (MTHFR) C677T genotype and levodopa treatment may give rise to elevated serum homocysteine levels in parkinsonian patients. We aimed to clarify the interplay of these factors in pathogenesis of Parkinson's disease (PD)-related hyperhomocysteinemia. Total serum levels of homocysteine (tHcy) and MTHFR C677T genotype were investigated in levodopa-treated and -untreated parkinsonian ("de novo") patients, as well as in control healthy subjects matched by age and gender (N=83, 30 and 53, respectively). MTHFR C677T genotypes were equally distributed in PD patients and control subjects, the T allele homozygosity being observed in app. 12-17% cases. tHcy concentrations were significantly higher in both levodopa-treated and -untreated PD patients than in control subjects, and in TT homozygotes than in CT or CC genotype carriers. tHcy levels significantly correlated with the duration of the disease in PD treated patients only, reaching the maximum after 3-6 years. However, there was no correlation between tHcy levels and total daily intake of levodopa in the same group of PD patients. In conclusion, MTHFR C677T genotype is a significant factor for hyperhomocysteinemia. in patients with PD, levodopa-untreated and probably even more in levodopa-treated PD patients.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Neurological Sciences
T1  - Homocysteine serum levels and MTHFR C677T genotype in patients with Parkinson's disease, with and without levodopa therapy
VL  - 248
IS  - 1-2
SP  - 56
EP  - 61
DO  - 10.1016/j.jns.2006.05.040
ER  - 

@article{
author = "Todorović, Zoran and Džoljić, Eleonora and Novaković, Ivana and Mirković, Duško and Stojanović, Radan and Nesić, Zorica and Krajinović, Maja and Prostran, Milica and Kostić, Vladimir",
year = "2006",
abstract = "Both methylenetetrahydrofolate (MTHFR) C677T genotype and levodopa treatment may give rise to elevated serum homocysteine levels in parkinsonian patients. We aimed to clarify the interplay of these factors in pathogenesis of Parkinson's disease (PD)-related hyperhomocysteinemia. Total serum levels of homocysteine (tHcy) and MTHFR C677T genotype were investigated in levodopa-treated and -untreated parkinsonian ("de novo") patients, as well as in control healthy subjects matched by age and gender (N=83, 30 and 53, respectively). MTHFR C677T genotypes were equally distributed in PD patients and control subjects, the T allele homozygosity being observed in app. 12-17% cases. tHcy concentrations were significantly higher in both levodopa-treated and -untreated PD patients than in control subjects, and in TT homozygotes than in CT or CC genotype carriers. tHcy levels significantly correlated with the duration of the disease in PD treated patients only, reaching the maximum after 3-6 years. However, there was no correlation between tHcy levels and total daily intake of levodopa in the same group of PD patients. In conclusion, MTHFR C677T genotype is a significant factor for hyperhomocysteinemia. in patients with PD, levodopa-untreated and probably even more in levodopa-treated PD patients.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Neurological Sciences",
title = "Homocysteine serum levels and MTHFR C677T genotype in patients with Parkinson's disease, with and without levodopa therapy",
volume = "248",
number = "1-2",
pages = "56-61",
doi = "10.1016/j.jns.2006.05.040"
}

Todorović, Z., Džoljić, E., Novaković, I., Mirković, D., Stojanović, R., Nesić, Z., Krajinović, M., Prostran, M.,& Kostić, V.. (2006). Homocysteine serum levels and MTHFR C677T genotype in patients with Parkinson's disease, with and without levodopa therapy. in Journal of the Neurological Sciences
Elsevier Science BV, Amsterdam., 248(1-2), 56-61.
https://doi.org/10.1016/j.jns.2006.05.040

Todorović Z, Džoljić E, Novaković I, Mirković D, Stojanović R, Nesić Z, Krajinović M, Prostran M, Kostić V. Homocysteine serum levels and MTHFR C677T genotype in patients with Parkinson's disease, with and without levodopa therapy. in Journal of the Neurological Sciences. 2006;248(1-2):56-61.
doi:10.1016/j.jns.2006.05.040 .

Todorović, Zoran, Džoljić, Eleonora, Novaković, Ivana, Mirković, Duško, Stojanović, Radan, Nesić, Zorica, Krajinović, Maja, Prostran, Milica, Kostić, Vladimir, "Homocysteine serum levels and MTHFR C677T genotype in patients with Parkinson's disease, with and without levodopa therapy" in Journal of the Neurological Sciences, 248, no. 1-2 (2006):56-61,
https://doi.org/10.1016/j.jns.2006.05.040 . .