TY  - JOUR
AU  - Kostić, Emilija
AU  - Đukić, Mirjana
AU  - Antović, Aleksandra
AU  - Zdravković, Miodrag
AU  - Milić, Miroslav
AU  - Stojanović, Ivan
AU  - Todorović, Stevan
AU  - Vujović, Maja
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4771
AB  - Background: The cannabis plant or marijuana has been used since ancient times for different purposes. An increase in the content of THC in cannabis has been observed worldwide, whereas the CBD content is dropping. This study’s main goal was to categorize marijuana suspected policies’ seizures (MSPS) based on the cannabinoid component concentrations concerning their potency and age. Methods: The samples were MSPS seized in southeast Serbia from April 2019 to April 2020. The cannabinoid content was determined using gas chromatography with mass spectrometry. Descriptive statistics were performed using Linux LibreOffice Calc. Results: All of the samples had a THC content higher than 0.3%, thus classifying the samples as marijuana according to the Serbian Law on Psychoactive Controlled Substances. The highest concentration of THC was 16.10%, while the lowest was 4.90%. The highest average concentration of THC (12.39%) was found in the buds. Only 11.81% of the samples had a CBD concentration higher than 1%, making all other marijuana samples extremely psychoactive. Most of the samples were older than 2 years, according to the calculated CBN/THC ratio. Conclusion: Our results confirm trends regarding the dominance of THC content in contemporary cannabis worldwide. It is necessary to monitor trends of cannabis potency in Serbia continuously because of its location.
PB  - MDPI
T2  - Separations
T1  - Categorization of Marijuana Suspected Policies’ Seizures in Southeast Serbia According to Cannabinoids Content
VL  - 10
IS  - 5
DO  - 10.3390/separations10050307
ER  - 

@article{
author = "Kostić, Emilija and Đukić, Mirjana and Antović, Aleksandra and Zdravković, Miodrag and Milić, Miroslav and Stojanović, Ivan and Todorović, Stevan and Vujović, Maja",
year = "2023",
abstract = "Background: The cannabis plant or marijuana has been used since ancient times for different purposes. An increase in the content of THC in cannabis has been observed worldwide, whereas the CBD content is dropping. This study’s main goal was to categorize marijuana suspected policies’ seizures (MSPS) based on the cannabinoid component concentrations concerning their potency and age. Methods: The samples were MSPS seized in southeast Serbia from April 2019 to April 2020. The cannabinoid content was determined using gas chromatography with mass spectrometry. Descriptive statistics were performed using Linux LibreOffice Calc. Results: All of the samples had a THC content higher than 0.3%, thus classifying the samples as marijuana according to the Serbian Law on Psychoactive Controlled Substances. The highest concentration of THC was 16.10%, while the lowest was 4.90%. The highest average concentration of THC (12.39%) was found in the buds. Only 11.81% of the samples had a CBD concentration higher than 1%, making all other marijuana samples extremely psychoactive. Most of the samples were older than 2 years, according to the calculated CBN/THC ratio. Conclusion: Our results confirm trends regarding the dominance of THC content in contemporary cannabis worldwide. It is necessary to monitor trends of cannabis potency in Serbia continuously because of its location.",
publisher = "MDPI",
journal = "Separations",
title = "Categorization of Marijuana Suspected Policies’ Seizures in Southeast Serbia According to Cannabinoids Content",
volume = "10",
number = "5",
doi = "10.3390/separations10050307"
}

Kostić, E., Đukić, M., Antović, A., Zdravković, M., Milić, M., Stojanović, I., Todorović, S.,& Vujović, M.. (2023). Categorization of Marijuana Suspected Policies’ Seizures in Southeast Serbia According to Cannabinoids Content. in Separations
MDPI., 10(5).
https://doi.org/10.3390/separations10050307

Kostić E, Đukić M, Antović A, Zdravković M, Milić M, Stojanović I, Todorović S, Vujović M. Categorization of Marijuana Suspected Policies’ Seizures in Southeast Serbia According to Cannabinoids Content. in Separations. 2023;10(5).
doi:10.3390/separations10050307 .

Kostić, Emilija, Đukić, Mirjana, Antović, Aleksandra, Zdravković, Miodrag, Milić, Miroslav, Stojanović, Ivan, Todorović, Stevan, Vujović, Maja, "Categorization of Marijuana Suspected Policies’ Seizures in Southeast Serbia According to Cannabinoids Content" in Separations, 10, no. 5 (2023),
https://doi.org/10.3390/separations10050307 . .

TY  - JOUR
AU  - Lalić-Ćosić, Sanja
AU  - Dopsaj, Violeta
AU  - Kovač, Mirjana
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Mobarrez, Fariborz
AU  - Antović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3997
AB  - Background: Pre-eclampsia (P-EC) is associated with systemic inflammation, endothelial dysfunction and hypercoagulability. The role of extracellular vesicles (EVs) in coagulation disturbances affecting the development and severity of P-EC remains elusive. We aimed to evaluate the concentration of EVs expressing phosphatidylserine (PS) and specific markers in relation to the thrombin and fibrin formation as well as fibrin clot properties, in pregnant women with P-EC in comparison to healthy pregnant women of similar gestational age. Methods: Blood samples of 30 pregnant women diagnosed with P-EC were collected on the morning following admission to hospital and after delivery (mean duration 5 days). The concentration of the PS-exposing EVs (PS+ EVs) from platelets (CD42a+, endothelial cells (CD62E+), and PS+ EVs expressing tissue factor (TF) and vascular cell adhesion molecule 1 (VCAM-1) were measured by flow cytometry. Further phenotyping of EVs also included expression of PlGF. Markers of maternal haemostasis were correlated with EVs concentration in plasma. Results: Preeclamptic pregnancy was associated with significantly higher plasma levels of PS+ CD42a+ EVs and PS+ VCAM-1+ EVs in comparison with normotensive pregnancy. P-EC patients after delivery had markedly elevated concentration of PS+ CD42a+ EVs, CD62E+ EVs, TF+ EVs, and VCAM-1+ EVs compared to those before delivery. Inverse correlation was observed between EVs concentrations (PS+, PS+ TF+, and PlGF+) and parameters of overall haemostatic potential (OHP) and fibrin formation, while PS+ VCAM-1+ EVs directly correlated with FVIII activity in plasma. Conclusion: Increased levels of PS+ EVs subpopulations in P-EC and their association with global haemostatic parameters, as well as with fibrin clot properties may suggest EVs involvement in intravascular fibrin deposition leading to subsequent microcirculation disorders.
PB  - Frontiers Media S.A.
T2  - Frontiers in Medicine
T1  - Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients
VL  - 8
DO  - 10.3389/fmed.2021.761453
ER  - 

@article{
author = "Lalić-Ćosić, Sanja and Dopsaj, Violeta and Kovač, Mirjana and Mandić-Marković, Vesna and Miković, Željko and Mobarrez, Fariborz and Antović, Aleksandra",
year = "2021",
abstract = "Background: Pre-eclampsia (P-EC) is associated with systemic inflammation, endothelial dysfunction and hypercoagulability. The role of extracellular vesicles (EVs) in coagulation disturbances affecting the development and severity of P-EC remains elusive. We aimed to evaluate the concentration of EVs expressing phosphatidylserine (PS) and specific markers in relation to the thrombin and fibrin formation as well as fibrin clot properties, in pregnant women with P-EC in comparison to healthy pregnant women of similar gestational age. Methods: Blood samples of 30 pregnant women diagnosed with P-EC were collected on the morning following admission to hospital and after delivery (mean duration 5 days). The concentration of the PS-exposing EVs (PS+ EVs) from platelets (CD42a+, endothelial cells (CD62E+), and PS+ EVs expressing tissue factor (TF) and vascular cell adhesion molecule 1 (VCAM-1) were measured by flow cytometry. Further phenotyping of EVs also included expression of PlGF. Markers of maternal haemostasis were correlated with EVs concentration in plasma. Results: Preeclamptic pregnancy was associated with significantly higher plasma levels of PS+ CD42a+ EVs and PS+ VCAM-1+ EVs in comparison with normotensive pregnancy. P-EC patients after delivery had markedly elevated concentration of PS+ CD42a+ EVs, CD62E+ EVs, TF+ EVs, and VCAM-1+ EVs compared to those before delivery. Inverse correlation was observed between EVs concentrations (PS+, PS+ TF+, and PlGF+) and parameters of overall haemostatic potential (OHP) and fibrin formation, while PS+ VCAM-1+ EVs directly correlated with FVIII activity in plasma. Conclusion: Increased levels of PS+ EVs subpopulations in P-EC and their association with global haemostatic parameters, as well as with fibrin clot properties may suggest EVs involvement in intravascular fibrin deposition leading to subsequent microcirculation disorders.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Medicine",
title = "Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients",
volume = "8",
doi = "10.3389/fmed.2021.761453"
}

Lalić-Ćosić, S., Dopsaj, V., Kovač, M., Mandić-Marković, V., Miković, Ž., Mobarrez, F.,& Antović, A.. (2021). Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients. in Frontiers in Medicine
Frontiers Media S.A.., 8.
https://doi.org/10.3389/fmed.2021.761453

Lalić-Ćosić S, Dopsaj V, Kovač M, Mandić-Marković V, Miković Ž, Mobarrez F, Antović A. Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients. in Frontiers in Medicine. 2021;8.
doi:10.3389/fmed.2021.761453 .

Lalić-Ćosić, Sanja, Dopsaj, Violeta, Kovač, Mirjana, Mandić-Marković, Vesna, Miković, Željko, Mobarrez, Fariborz, Antović, Aleksandra, "Phosphatidylserine Exposing Extracellular Vesicles in Pre-eclamptic Patients" in Frontiers in Medicine, 8 (2021),
https://doi.org/10.3389/fmed.2021.761453 . .

TY  - JOUR
AU  - Lalić-Ćosić, Sanja
AU  - Dopsaj, Violeta
AU  - Kovač, Mirjana
AU  - Pruner, Iva
AU  - Littmann, Karin
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Antović, Aleksandra
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3609
AB  - Introduction: Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods: Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays—endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)—were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results: Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP—determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion: Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.
PB  - Wiley-Blackwell
T2  - International Journal of Laboratory Hematology
T1  - Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia
VL  - 42
IS  - 3
SP  - 322
EP  - 330
DO  - 10.1111/ijlh.13183
ER  - 

@article{
author = "Lalić-Ćosić, Sanja and Dopsaj, Violeta and Kovač, Mirjana and Pruner, Iva and Littmann, Karin and Mandić-Marković, Vesna and Miković, Željko and Antović, Aleksandra",
year = "2020",
abstract = "Introduction: Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods: Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays—endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)—were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results: Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP—determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion: Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.",
publisher = "Wiley-Blackwell",
journal = "International Journal of Laboratory Hematology",
title = "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia",
volume = "42",
number = "3",
pages = "322-330",
doi = "10.1111/ijlh.13183"
}

Lalić-Ćosić, S., Dopsaj, V., Kovač, M., Pruner, I., Littmann, K., Mandić-Marković, V., Miković, Ž.,& Antović, A.. (2020). Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology
Wiley-Blackwell., 42(3), 322-330.
https://doi.org/10.1111/ijlh.13183

Lalić-Ćosić S, Dopsaj V, Kovač M, Pruner I, Littmann K, Mandić-Marković V, Miković Ž, Antović A. Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology. 2020;42(3):322-330.
doi:10.1111/ijlh.13183 .

Lalić-Ćosić, Sanja, Dopsaj, Violeta, Kovač, Mirjana, Pruner, Iva, Littmann, Karin, Mandić-Marković, Vesna, Miković, Željko, Antović, Aleksandra, "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia" in International Journal of Laboratory Hematology, 42, no. 3 (2020):322-330,
https://doi.org/10.1111/ijlh.13183 . .

TY  - JOUR
AU  - Vucelić, Dragica
AU  - Jesić, Rada
AU  - Jovičić, Snežana
AU  - Zivotić, Maja
AU  - Grubor, Nikica
AU  - Trajković, Goran
AU  - Canić, Ivana
AU  - Elezović, Ivo
AU  - Antović, Aleksandra
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2396
AB  - Background: The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing information on both quantitative deficit and fibrin polymerization disorders. Objective: To compare these three methods of assessing fibrinogen in patients with cirrhosis of different aetiologies, characterized by impairment in fibrinogen concentration as well as functional aberrance. Methods: Sixty patients with alcoholic and 24 patients with cholestatic cirrhosis were included (Child-Pugh score (CPs) A, n = 24; B, n = 32; C, n = 28). All parameters were compared with those from a control group. Maximum clot firmness (MCF) in the FIBTEM test was assessed in regard to its relevance in detection of qualitative fibrinogen disorders in comparison with results obtained by standard measurement methods, i.e. the Clauss fibrinogen method and TCT. Results: With increased cirrhosis severity, fibrinogen and FIBTEM-MCF levels significantly declined (p = 0.002), while TCT was significantly prolonged (p = 0.002). In all CPs groups, fibrinogen strongly correlated with FIBTEM-MCF (r = 0.77, r = 0.72, r = 0.74; p  lt  0.001), while cross-correlations of other assays were highly variable. The prevalence of decreased FIBTEM-MCF values ( lt 9 mm) was significantly higher in advanced CPs categories (p = 0.027), whereby the highest prevalence was detected in patients with CPsC (10/16; 62.5%). Nine of the 16 patients with decreased FIBTEM-MCF values had also decreased fibrinogen levels, while in the remaining 7 patients fibrinogen levels were within the reference range, indicating the possible presence of qualitatively altered fibrinogen that could be detected by FIBTEM-MCF. Conclusions: FIBTEM-MCF may be considered as a reliable alternative to standard plasma fibrinogen measurement in cirrhotic patients, especially in evaluating fibrin polymerization disorders in these patients. Further studies are needed to evaluate the usefulness of this assay in predicting bleeding complications in cirrhotic patients as well as monitoring replacement treatment.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Tohoku Journal of Experimental Medicine
T1  - Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis
VL  - 135
IS  - 6
SP  - 1124
EP  - 1130
DO  - 10.1016/j.thromres.2015.04.003
ER  - 

@article{
author = "Vucelić, Dragica and Jesić, Rada and Jovičić, Snežana and Zivotić, Maja and Grubor, Nikica and Trajković, Goran and Canić, Ivana and Elezović, Ivo and Antović, Aleksandra",
year = "2015",
abstract = "Background: The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing information on both quantitative deficit and fibrin polymerization disorders. Objective: To compare these three methods of assessing fibrinogen in patients with cirrhosis of different aetiologies, characterized by impairment in fibrinogen concentration as well as functional aberrance. Methods: Sixty patients with alcoholic and 24 patients with cholestatic cirrhosis were included (Child-Pugh score (CPs) A, n = 24; B, n = 32; C, n = 28). All parameters were compared with those from a control group. Maximum clot firmness (MCF) in the FIBTEM test was assessed in regard to its relevance in detection of qualitative fibrinogen disorders in comparison with results obtained by standard measurement methods, i.e. the Clauss fibrinogen method and TCT. Results: With increased cirrhosis severity, fibrinogen and FIBTEM-MCF levels significantly declined (p = 0.002), while TCT was significantly prolonged (p = 0.002). In all CPs groups, fibrinogen strongly correlated with FIBTEM-MCF (r = 0.77, r = 0.72, r = 0.74; p  lt  0.001), while cross-correlations of other assays were highly variable. The prevalence of decreased FIBTEM-MCF values ( lt 9 mm) was significantly higher in advanced CPs categories (p = 0.027), whereby the highest prevalence was detected in patients with CPsC (10/16; 62.5%). Nine of the 16 patients with decreased FIBTEM-MCF values had also decreased fibrinogen levels, while in the remaining 7 patients fibrinogen levels were within the reference range, indicating the possible presence of qualitatively altered fibrinogen that could be detected by FIBTEM-MCF. Conclusions: FIBTEM-MCF may be considered as a reliable alternative to standard plasma fibrinogen measurement in cirrhotic patients, especially in evaluating fibrin polymerization disorders in these patients. Further studies are needed to evaluate the usefulness of this assay in predicting bleeding complications in cirrhotic patients as well as monitoring replacement treatment.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Tohoku Journal of Experimental Medicine",
title = "Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis",
volume = "135",
number = "6",
pages = "1124-1130",
doi = "10.1016/j.thromres.2015.04.003"
}

Vucelić, D., Jesić, R., Jovičić, S., Zivotić, M., Grubor, N., Trajković, G., Canić, I., Elezović, I.,& Antović, A.. (2015). Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis. in Tohoku Journal of Experimental Medicine
Pergamon-Elsevier Science Ltd, Oxford., 135(6), 1124-1130.
https://doi.org/10.1016/j.thromres.2015.04.003

Vucelić D, Jesić R, Jovičić S, Zivotić M, Grubor N, Trajković G, Canić I, Elezović I, Antović A. Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis. in Tohoku Journal of Experimental Medicine. 2015;135(6):1124-1130.
doi:10.1016/j.thromres.2015.04.003 .

Vucelić, Dragica, Jesić, Rada, Jovičić, Snežana, Zivotić, Maja, Grubor, Nikica, Trajković, Goran, Canić, Ivana, Elezović, Ivo, Antović, Aleksandra, "Comparison of standard fibrinogen measurement methods with fibrin clot firmness assessed by thromboelastometry in patients with cirrhosis" in Tohoku Journal of Experimental Medicine, 135, no. 6 (2015):1124-1130,
https://doi.org/10.1016/j.thromres.2015.04.003 . .