TY  - JOUR
AU  - Ćulafić, Milica
AU  - Vezmar-Kovačević, Sandra
AU  - Dopsaj, Violeta
AU  - Stulić, Miloš
AU  - Vlaisavljević, Željko
AU  - Miljković, Branislava
AU  - Ćulafić, Đorđe
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3262
AB  - Background and objectives: Data suggests that nearly 30% of the general population have steatosis and up to 5% of this population develops nonalcoholic steatohepatitis (NASH). Liver biopsy is still considered to be the gold standard for the diagnosis of NASH. Great effort is being made toward the identification of sensitive diagnostic tests that do not involve invasive procedures to address a common concern in patients with the nonalcoholic fatty liver disease-whether they have NASH or simple steatosis. We aimed to investigate the independent predictors and develop a non-invasive, easy-to-perform, low-cost set of parameters that may be used in clinical practice to differentiate simple steatosis from NASH. Methods: А cross-sectional study of nonalcoholic fatty liver disease (NAFLD) patients divided into two groups: group I-simple steatosis (SS) and group II-biopsy-proven NASH. Strict inclusion criteria and stepwise analysis allowed the evaluation of a vast number of measured/estimated parameters. Results: One hundred and eleven patients were included-82 with simple steatosis and 29 with biopsy-proven NASH. The probability of NASH was the highest when homeostatic model assessment of insulin resistance (HOMA-IR) was above 2.5, uric acid above 380 µmol/L, ferritin above 100 µg/L and ALT above 45 U/L. An acronym of using first letters was created and named the HUFA index. This combined model resulted in an area under the receiver operator characteristic curve (AUROC) of 0.94, provided sensitivity, specificity, positive predictive value and a negative predictive value for NASH of 70.3%, 95.1%, 83.1% and 90.0%, respectively. Conclusion: We suggest a simple non-invasive predictive index HUFA that encompasses four easily available parameters (HOMA-IR, uric acid, ferritin and ALT) to identify patients with NASH, which may reduce the need for a liver biopsy on a routine basis in patients with NAFLD.
PB  - NLM (Medline)
T2  - Medicina (Kaunas, Lithuania)
T1  - A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests
VL  - 55
IS  - 6
DO  - 10.3390/medicina55060243
ER  - 

@article{
author = "Ćulafić, Milica and Vezmar-Kovačević, Sandra and Dopsaj, Violeta and Stulić, Miloš and Vlaisavljević, Željko and Miljković, Branislava and Ćulafić, Đorđe",
year = "2019",
abstract = "Background and objectives: Data suggests that nearly 30% of the general population have steatosis and up to 5% of this population develops nonalcoholic steatohepatitis (NASH). Liver biopsy is still considered to be the gold standard for the diagnosis of NASH. Great effort is being made toward the identification of sensitive diagnostic tests that do not involve invasive procedures to address a common concern in patients with the nonalcoholic fatty liver disease-whether they have NASH or simple steatosis. We aimed to investigate the independent predictors and develop a non-invasive, easy-to-perform, low-cost set of parameters that may be used in clinical practice to differentiate simple steatosis from NASH. Methods: А cross-sectional study of nonalcoholic fatty liver disease (NAFLD) patients divided into two groups: group I-simple steatosis (SS) and group II-biopsy-proven NASH. Strict inclusion criteria and stepwise analysis allowed the evaluation of a vast number of measured/estimated parameters. Results: One hundred and eleven patients were included-82 with simple steatosis and 29 with biopsy-proven NASH. The probability of NASH was the highest when homeostatic model assessment of insulin resistance (HOMA-IR) was above 2.5, uric acid above 380 µmol/L, ferritin above 100 µg/L and ALT above 45 U/L. An acronym of using first letters was created and named the HUFA index. This combined model resulted in an area under the receiver operator characteristic curve (AUROC) of 0.94, provided sensitivity, specificity, positive predictive value and a negative predictive value for NASH of 70.3%, 95.1%, 83.1% and 90.0%, respectively. Conclusion: We suggest a simple non-invasive predictive index HUFA that encompasses four easily available parameters (HOMA-IR, uric acid, ferritin and ALT) to identify patients with NASH, which may reduce the need for a liver biopsy on a routine basis in patients with NAFLD.",
publisher = "NLM (Medline)",
journal = "Medicina (Kaunas, Lithuania)",
title = "A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests",
volume = "55",
number = "6",
doi = "10.3390/medicina55060243"
}

Ćulafić, M., Vezmar-Kovačević, S., Dopsaj, V., Stulić, M., Vlaisavljević, Ž., Miljković, B.,& Ćulafić, Đ.. (2019). A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests. in Medicina (Kaunas, Lithuania)
NLM (Medline)., 55(6).
https://doi.org/10.3390/medicina55060243

Ćulafić M, Vezmar-Kovačević S, Dopsaj V, Stulić M, Vlaisavljević Ž, Miljković B, Ćulafić Đ. A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests. in Medicina (Kaunas, Lithuania). 2019;55(6).
doi:10.3390/medicina55060243 .

Ćulafić, Milica, Vezmar-Kovačević, Sandra, Dopsaj, Violeta, Stulić, Miloš, Vlaisavljević, Željko, Miljković, Branislava, Ćulafić, Đorđe, "A Simple Index for Nonalcoholic Steatohepatitis-HUFA-Based on Routinely Performed Blood Tests" in Medicina (Kaunas, Lithuania), 55, no. 6 (2019),
https://doi.org/10.3390/medicina55060243 . .

TY  - JOUR
AU  - Stulić, Miloš
AU  - Ćulafić, Đorđe
AU  - Obrenović, Radmila
AU  - Janković, Goran
AU  - Alempijević, Tamara
AU  - Stojković-Lalošević, Milica
AU  - Dostanić, Nataša
AU  - Vezmar-Kovačević, Sandra
AU  - Ćulafić, Milica
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3068
AB  - Background: Data suggest cystatin C (CysC) levels and hepatic artery resistive index (HARI) correspond to the progression of chronic liver disease. We aimed to evaluate the clinical significance of these parameters in assessment of fibrosis in patients with liver cirrhosis. Methods: The cross-sectional study included 63 patients with liver cirrhosis. A control group consisted of 30 age- and gender-matched healthy persons. Results: We confirmed significantly higher values of CysC in patients with cirrhosis compared to control group (p = 0.036). Average value of HARI in the examined group was increased (0.72 +/- 0.06) and there was the statistically significant difference compared to controls (0.66 +/- 0.03) (p  lt  0.001). We found statistically significant correlation between HARI and CysC in the study group. Analyzing the possibility of distinguishing healthy subjects from patients with fibrosis, we have found that the area under the curve is far greater in the HARI index than CysC. Comparison of CysC among Child-Pugh stages and correlation with a model for end-stage liver disease (MELD) score showed statistically significant results. Conclusion: We confirmed HARI is a more accurate parameter than CysC in discriminating healthy subjects from patients with fibrosis, while CysC could be a better indicator of the stage of liver cirrhosis.
PB  - MDPI, Basel
T2  - Medicina-Lithuania
T1  - The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis
VL  - 54
IS  - 3
DO  - 10.3390/medicina54030037
ER  - 

@article{
author = "Stulić, Miloš and Ćulafić, Đorđe and Obrenović, Radmila and Janković, Goran and Alempijević, Tamara and Stojković-Lalošević, Milica and Dostanić, Nataša and Vezmar-Kovačević, Sandra and Ćulafić, Milica",
year = "2018",
abstract = "Background: Data suggest cystatin C (CysC) levels and hepatic artery resistive index (HARI) correspond to the progression of chronic liver disease. We aimed to evaluate the clinical significance of these parameters in assessment of fibrosis in patients with liver cirrhosis. Methods: The cross-sectional study included 63 patients with liver cirrhosis. A control group consisted of 30 age- and gender-matched healthy persons. Results: We confirmed significantly higher values of CysC in patients with cirrhosis compared to control group (p = 0.036). Average value of HARI in the examined group was increased (0.72 +/- 0.06) and there was the statistically significant difference compared to controls (0.66 +/- 0.03) (p  lt  0.001). We found statistically significant correlation between HARI and CysC in the study group. Analyzing the possibility of distinguishing healthy subjects from patients with fibrosis, we have found that the area under the curve is far greater in the HARI index than CysC. Comparison of CysC among Child-Pugh stages and correlation with a model for end-stage liver disease (MELD) score showed statistically significant results. Conclusion: We confirmed HARI is a more accurate parameter than CysC in discriminating healthy subjects from patients with fibrosis, while CysC could be a better indicator of the stage of liver cirrhosis.",
publisher = "MDPI, Basel",
journal = "Medicina-Lithuania",
title = "The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis",
volume = "54",
number = "3",
doi = "10.3390/medicina54030037"
}

Stulić, M., Ćulafić, Đ., Obrenović, R., Janković, G., Alempijević, T., Stojković-Lalošević, M., Dostanić, N., Vezmar-Kovačević, S.,& Ćulafić, M.. (2018). The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis. in Medicina-Lithuania
MDPI, Basel., 54(3).
https://doi.org/10.3390/medicina54030037

Stulić M, Ćulafić Đ, Obrenović R, Janković G, Alempijević T, Stojković-Lalošević M, Dostanić N, Vezmar-Kovačević S, Ćulafić M. The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis. in Medicina-Lithuania. 2018;54(3).
doi:10.3390/medicina54030037 .

Stulić, Miloš, Ćulafić, Đorđe, Obrenović, Radmila, Janković, Goran, Alempijević, Tamara, Stojković-Lalošević, Milica, Dostanić, Nataša, Vezmar-Kovačević, Sandra, Ćulafić, Milica, "The Clinical Importance of Cystatin C and Hepatic Artery Resistive Index in Liver Cirrhosis" in Medicina-Lithuania, 54, no. 3 (2018),
https://doi.org/10.3390/medicina54030037 . .

TY  - CONF
AU  - Ćulafić, Milica
AU  - Vezmar-Kovačević, Sandra
AU  - Stulić, Miloš
AU  - Krstić, Aleksandra
AU  - Vučićević, Katarina
AU  - Miljković, Branislava
AU  - Ćulafić, Đorđe
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2452
PB  - Wiley-Blackwell, Hoboken
C3  - Pharmacotherapy
T1  - Pentoxifylline in combination with metformin in the treatment of non-alcoholic steatohepatitis.
VL  - 35
IS  - 5
SP  - e69
EP  - e69
DO  - 10.1002/phar.1606
ER  - 

@conference{
author = "Ćulafić, Milica and Vezmar-Kovačević, Sandra and Stulić, Miloš and Krstić, Aleksandra and Vučićević, Katarina and Miljković, Branislava and Ćulafić, Đorđe",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Pharmacotherapy",
title = "Pentoxifylline in combination with metformin in the treatment of non-alcoholic steatohepatitis.",
volume = "35",
number = "5",
pages = "e69-e69",
doi = "10.1002/phar.1606"
}

Ćulafić, M., Vezmar-Kovačević, S., Stulić, M., Krstić, A., Vučićević, K., Miljković, B.,& Ćulafić, Đ.. (2015). Pentoxifylline in combination with metformin in the treatment of non-alcoholic steatohepatitis.. in Pharmacotherapy
Wiley-Blackwell, Hoboken., 35(5), e69-e69.
https://doi.org/10.1002/phar.1606

Ćulafić M, Vezmar-Kovačević S, Stulić M, Krstić A, Vučićević K, Miljković B, Ćulafić Đ. Pentoxifylline in combination with metformin in the treatment of non-alcoholic steatohepatitis.. in Pharmacotherapy. 2015;35(5):e69-e69.
doi:10.1002/phar.1606 .

Ćulafić, Milica, Vezmar-Kovačević, Sandra, Stulić, Miloš, Krstić, Aleksandra, Vučićević, Katarina, Miljković, Branislava, Ćulafić, Đorđe, "Pentoxifylline in combination with metformin in the treatment of non-alcoholic steatohepatitis." in Pharmacotherapy, 35, no. 5 (2015):e69-e69,
https://doi.org/10.1002/phar.1606 . .

TY  - JOUR
AU  - Culafić-Vojinović, Violeta
AU  - Ćulafić, Đorđe
AU  - Ignjatović, Svetlana
AU  - Petakov, Milan
AU  - Đurović-Nikolić, Marina
AU  - Vasić, Jelena
AU  - Mirković, Duško
AU  - Mijac, Dragana
AU  - Stulić, Miloš
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2146
AB  - Background: Metabolic bone disease in patients with chronic liver disease is called hepatic osteodystrophy and is primarily a sequel to osteopenia/osteoporosis, and rarely secondary to osteomalacia: The aim of this work was to define the influence of vitamin D-3 and parathyroid hormone (PTH) in the pathogenesis of hepatic osteodystrophy, as well as the predictive significance of biochemical bone markers. Methods: This prospective study included 58 male patients with alcoholic (49) and viral (9) cirrhosis. The concentrations of serum vitamin D-3, PTH, osteocalcin and beta-carboxy-terminal cross-linked telopeptide of type I collagen (beta-CTX) were determined. Bone density was measured by dual energy X-ray absorptiometry in the L1 -L4 spinal segment and the femoral neck. Results: Lower bone mineral density (BMD) was measured in 41 patients (70.7%). There was no significant correlation between PTH and vitamin D3 values and T score in the femoral neck (p=0.51; p=0.063) and lumbar spine (p=0.49; 0.064). Also, no significant correlation was found between the osteocalcin values in lumbar spine BMD (p=0.944) and femoral neck (p=0.161), or with beta-CTX values and BMD in the lumbar spine (p=0.347) and femoral neck (p=0.73). Statistically significant difference was confirmed between the stage A osteocalcin (p=0.000) and beta-CTX (p=0.008) values in relation to advanced stages B and C. Conclusions: PTH and vitamin D3 do not influence the development of hepatic osteodystrophy. In patients with cirrhosis, osteocalcin and beta-CTX are not valid indicators of decreased BMD, but their values correlate with the degree of liver insufficiency.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis
VL  - 33
IS  - 2
SP  - 149
EP  - 155
DO  - 10.2478/jomb-2013-0025
ER  - 

@article{
author = "Culafić-Vojinović, Violeta and Ćulafić, Đorđe and Ignjatović, Svetlana and Petakov, Milan and Đurović-Nikolić, Marina and Vasić, Jelena and Mirković, Duško and Mijac, Dragana and Stulić, Miloš",
year = "2014",
abstract = "Background: Metabolic bone disease in patients with chronic liver disease is called hepatic osteodystrophy and is primarily a sequel to osteopenia/osteoporosis, and rarely secondary to osteomalacia: The aim of this work was to define the influence of vitamin D-3 and parathyroid hormone (PTH) in the pathogenesis of hepatic osteodystrophy, as well as the predictive significance of biochemical bone markers. Methods: This prospective study included 58 male patients with alcoholic (49) and viral (9) cirrhosis. The concentrations of serum vitamin D-3, PTH, osteocalcin and beta-carboxy-terminal cross-linked telopeptide of type I collagen (beta-CTX) were determined. Bone density was measured by dual energy X-ray absorptiometry in the L1 -L4 spinal segment and the femoral neck. Results: Lower bone mineral density (BMD) was measured in 41 patients (70.7%). There was no significant correlation between PTH and vitamin D3 values and T score in the femoral neck (p=0.51; p=0.063) and lumbar spine (p=0.49; 0.064). Also, no significant correlation was found between the osteocalcin values in lumbar spine BMD (p=0.944) and femoral neck (p=0.161), or with beta-CTX values and BMD in the lumbar spine (p=0.347) and femoral neck (p=0.73). Statistically significant difference was confirmed between the stage A osteocalcin (p=0.000) and beta-CTX (p=0.008) values in relation to advanced stages B and C. Conclusions: PTH and vitamin D3 do not influence the development of hepatic osteodystrophy. In patients with cirrhosis, osteocalcin and beta-CTX are not valid indicators of decreased BMD, but their values correlate with the degree of liver insufficiency.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis",
volume = "33",
number = "2",
pages = "149-155",
doi = "10.2478/jomb-2013-0025"
}

Culafić-Vojinović, V., Ćulafić, Đ., Ignjatović, S., Petakov, M., Đurović-Nikolić, M., Vasić, J., Mirković, D., Mijac, D.,& Stulić, M.. (2014). The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 33(2), 149-155.
https://doi.org/10.2478/jomb-2013-0025

Culafić-Vojinović V, Ćulafić Đ, Ignjatović S, Petakov M, Đurović-Nikolić M, Vasić J, Mirković D, Mijac D, Stulić M. The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis. in Journal of Medical Biochemistry. 2014;33(2):149-155.
doi:10.2478/jomb-2013-0025 .

Culafić-Vojinović, Violeta, Ćulafić, Đorđe, Ignjatović, Svetlana, Petakov, Milan, Đurović-Nikolić, Marina, Vasić, Jelena, Mirković, Duško, Mijac, Dragana, Stulić, Miloš, "The clinical importance of biochemical bone markers in patients with alcoholic and viral liver cirrhosis" in Journal of Medical Biochemistry, 33, no. 2 (2014):149-155,
https://doi.org/10.2478/jomb-2013-0025 . .

TY  - JOUR
AU  - Ćulafić, Đorđe
AU  - Stulić, Miloš
AU  - Obrenović, Radmila
AU  - Miletić, Danijela
AU  - Mijac, Dragana
AU  - Stojković, Milica
AU  - Jovanović, Marija
AU  - Ćulafić, Milica
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2213
AB  - AIM: To evaluate the clinical significance of cystatin C and renal resistive index for the determination of renal function in patients with liver cirrhosis. METHODS: We conducted a study of 63 patients with liver cirrhosis. A control group comprised of 30 age and gender-matched healthy persons. Serum cystatin C was determined in all study subjects and renal Doppler ultrasonography was made. Estimated glomerular filtration rate from serum creatinine (GFR(Cr)) and cystatin C (GFR(Cys)) was calculated. RESULTS: We confirmed significant differences in values of cystatin C between patients with different stages of liver cirrhosis according to Child-Pugh (P = 0.01), and a significant correlation with model of end stage liver disease (MELD) score (r(s) = 0.527, P  lt  0.001). More patients with decreased glomerular filtration rate were identified based on GFR(Cys) than on GFR(Cr) (P  lt  0.001). Significantly higher renal resistive index was noted in Child-Pugh C than in A (P  lt  0.001) and B stage (P = 0.001). Also, a significant correlation between renal resistive index and MELD score was observed (r(s) = 0.607, P  lt  0.001). Renal resistive index correlated significantly with cystatin C (r(s) = 0.283, P = 0.028) and showed a negative correlation with GFR(Cys) (r(s) = -0.31, P = 0.016). CONCLUSION: Cystatin C may be a more reliable marker for assessment of liver insufficiency. Additionally, cystatin C and renal resistive index represent sensitive indicators of renal dysfunction in patients with liver cirrhosis.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Microbiology & Biotechnology
T1  - Role of cystatin C and renal resistive index in assessment of renal function in patients with liver cirrhosis
VL  - 20
IS  - 21
SP  - 6573
EP  - 6579
DO  - 10.3748/wjg.v20.i21.6573
ER  - 

@article{
author = "Ćulafić, Đorđe and Stulić, Miloš and Obrenović, Radmila and Miletić, Danijela and Mijac, Dragana and Stojković, Milica and Jovanović, Marija and Ćulafić, Milica",
year = "2014",
abstract = "AIM: To evaluate the clinical significance of cystatin C and renal resistive index for the determination of renal function in patients with liver cirrhosis. METHODS: We conducted a study of 63 patients with liver cirrhosis. A control group comprised of 30 age and gender-matched healthy persons. Serum cystatin C was determined in all study subjects and renal Doppler ultrasonography was made. Estimated glomerular filtration rate from serum creatinine (GFR(Cr)) and cystatin C (GFR(Cys)) was calculated. RESULTS: We confirmed significant differences in values of cystatin C between patients with different stages of liver cirrhosis according to Child-Pugh (P = 0.01), and a significant correlation with model of end stage liver disease (MELD) score (r(s) = 0.527, P  lt  0.001). More patients with decreased glomerular filtration rate were identified based on GFR(Cys) than on GFR(Cr) (P  lt  0.001). Significantly higher renal resistive index was noted in Child-Pugh C than in A (P  lt  0.001) and B stage (P = 0.001). Also, a significant correlation between renal resistive index and MELD score was observed (r(s) = 0.607, P  lt  0.001). Renal resistive index correlated significantly with cystatin C (r(s) = 0.283, P = 0.028) and showed a negative correlation with GFR(Cys) (r(s) = -0.31, P = 0.016). CONCLUSION: Cystatin C may be a more reliable marker for assessment of liver insufficiency. Additionally, cystatin C and renal resistive index represent sensitive indicators of renal dysfunction in patients with liver cirrhosis.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Microbiology & Biotechnology",
title = "Role of cystatin C and renal resistive index in assessment of renal function in patients with liver cirrhosis",
volume = "20",
number = "21",
pages = "6573-6579",
doi = "10.3748/wjg.v20.i21.6573"
}

Ćulafić, Đ., Stulić, M., Obrenović, R., Miletić, D., Mijac, D., Stojković, M., Jovanović, M.,& Ćulafić, M.. (2014). Role of cystatin C and renal resistive index in assessment of renal function in patients with liver cirrhosis. in World Journal of Microbiology & Biotechnology
Baishideng Publishing Group Inc, Pleasanton., 20(21), 6573-6579.
https://doi.org/10.3748/wjg.v20.i21.6573

Ćulafić Đ, Stulić M, Obrenović R, Miletić D, Mijac D, Stojković M, Jovanović M, Ćulafić M. Role of cystatin C and renal resistive index in assessment of renal function in patients with liver cirrhosis. in World Journal of Microbiology & Biotechnology. 2014;20(21):6573-6579.
doi:10.3748/wjg.v20.i21.6573 .

Ćulafić, Đorđe, Stulić, Miloš, Obrenović, Radmila, Miletić, Danijela, Mijac, Dragana, Stojković, Milica, Jovanović, Marija, Ćulafić, Milica, "Role of cystatin C and renal resistive index in assessment of renal function in patients with liver cirrhosis" in World Journal of Microbiology & Biotechnology, 20, no. 21 (2014):6573-6579,
https://doi.org/10.3748/wjg.v20.i21.6573 . .