TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2803
AB  - The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.
PB  - Univ South Bohemia, Ceska Budejovice
T2  - Journal of Applied Biomedicine
T1  - Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function
VL  - 15
IS  - 3
SP  - 204
EP  - 209
DO  - 10.1016/j.jab.2017.02.002
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2017",
abstract = "The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.",
publisher = "Univ South Bohemia, Ceska Budejovice",
journal = "Journal of Applied Biomedicine",
title = "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function",
volume = "15",
number = "3",
pages = "204-209",
doi = "10.1016/j.jab.2017.02.002"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2017). Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine
Univ South Bohemia, Ceska Budejovice., 15(3), 204-209.
https://doi.org/10.1016/j.jab.2017.02.002

Totosković D, Dopsaj V, Martinović J. Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine. 2017;15(3):204-209.
doi:10.1016/j.jab.2017.02.002 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function" in Journal of Applied Biomedicine, 15, no. 3 (2017):204-209,
https://doi.org/10.1016/j.jab.2017.02.002 . .

TY  - CONF
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2697
PB  - Wiley-Blackwell, Hoboken
C3  - International Journal of Laboratory Hematology
T1  - Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment
VL  - 38
SP  - 107
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2697
ER  - 

@conference{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment",
volume = "38",
pages = "107-108",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2697"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 38, 107-108.
https://hdl.handle.net/21.15107/rcub_farfar_2697

Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment. in International Journal of Laboratory Hematology. 2016;38:107-108.
https://hdl.handle.net/21.15107/rcub_farfar_2697 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in cobalamin status assessment" in International Journal of Laboratory Hematology, 38 (2016):107-108,
https://hdl.handle.net/21.15107/rcub_farfar_2697 .

TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2589
AB  - IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis
VL  - 38
IS  - 3
SP  - 265
EP  - 272
DO  - 10.1111/ijlh.12479
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2016",
abstract = "IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis",
volume = "38",
number = "3",
pages = "265-272",
doi = "10.1111/ijlh.12479"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 38(3), 265-272.
https://doi.org/10.1111/ijlh.12479

Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology. 2016;38(3):265-272.
doi:10.1111/ijlh.12479 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis" in International Journal of Laboratory Hematology, 38, no. 3 (2016):265-272,
https://doi.org/10.1111/ijlh.12479 . .