TY  - JOUR
AU  - Samardžić, Stevan
AU  - Tucović, Aleksandra
AU  - Stojanović, Danilo
AU  - Maksimović, Zoran
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4663
AB  - Fruticin (amorphin) is a constituent of the fruit of the false indigo-bush (Amorpha fruticosa L.), which
belongs to the class of rotenoid glycosides, and shows several interesting pharmacological activities.
The aim of this study was to isolate and chemically characterize this natural product, as well as to
determine the optimal period of the year for A. fruticosa fruits collection. Fruticin was obtained by
re-crystallization of the precipitate that formed after partial evaporation of the extract, prepared by 3-
fold extraction of powdered plant material by chloroform - ethanol (1:1, v/v). The structure of the final
product was determined by various techniques of instrumental analysis (NMR, UV and MS), and con-
firmed by comparing the obtained spectra with corresponding data in available literature. The content
of fruticin in A. fruticosa fruit was determined by LC-DAD-MS, using the external standard method
based on the constructed calibration curve. Limits of detection (LOD) and quantification (LOQ) were
also determined. A substantial increase in fruticin content was observed during the ripening period
(>50 %). It has also been established that the optimal time for fruit collection is mid-December. Ob-
tained results indicate that the content of fruticin in the A. fruticosa fruit is highly dependable on the
time of collection. Since the biosynthesis of secondary plant metabolites is influenced not only by the
time of collection, but by numerous other factors as well, additional studies are needed to define, with
greater certainty, the conditions that are necessary for design of prospective efficient and sustainable
production process.
PB  - Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia
VL  - 40
SP  - 52
EP  - 56
DO  - 10.5937/leksir2040052S
ER  - 

@article{
author = "Samardžić, Stevan and Tucović, Aleksandra and Stojanović, Danilo and Maksimović, Zoran",
year = "2020",
abstract = "Fruticin (amorphin) is a constituent of the fruit of the false indigo-bush (Amorpha fruticosa L.), which
belongs to the class of rotenoid glycosides, and shows several interesting pharmacological activities.
The aim of this study was to isolate and chemically characterize this natural product, as well as to
determine the optimal period of the year for A. fruticosa fruits collection. Fruticin was obtained by
re-crystallization of the precipitate that formed after partial evaporation of the extract, prepared by 3-
fold extraction of powdered plant material by chloroform - ethanol (1:1, v/v). The structure of the final
product was determined by various techniques of instrumental analysis (NMR, UV and MS), and con-
firmed by comparing the obtained spectra with corresponding data in available literature. The content
of fruticin in A. fruticosa fruit was determined by LC-DAD-MS, using the external standard method
based on the constructed calibration curve. Limits of detection (LOD) and quantification (LOQ) were
also determined. A substantial increase in fruticin content was observed during the ripening period
(>50 %). It has also been established that the optimal time for fruit collection is mid-December. Ob-
tained results indicate that the content of fruticin in the A. fruticosa fruit is highly dependable on the
time of collection. Since the biosynthesis of secondary plant metabolites is influenced not only by the
time of collection, but by numerous other factors as well, additional studies are needed to define, with
greater certainty, the conditions that are necessary for design of prospective efficient and sustainable
production process.",
publisher = "Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia",
volume = "40",
pages = "52-56",
doi = "10.5937/leksir2040052S"
}

Samardžić, S., Tucović, A., Stojanović, D.,& Maksimović, Z.. (2020). Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd., 40, 52-56.
https://doi.org/10.5937/leksir2040052S

Samardžić S, Tucović A, Stojanović D, Maksimović Z. Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia. in Lekovite sirovine. 2020;40:52-56.
doi:10.5937/leksir2040052S .

Samardžić, Stevan, Tucović, Aleksandra, Stojanović, Danilo, Maksimović, Zoran, "Isolation and seasonal variation of fruticin in fruits of false indigo-bush (Amorpha fruticosa L. Fabaceae) from Serbia" in Lekovite sirovine, 40 (2020):52-56,
https://doi.org/10.5937/leksir2040052S . .