Zeković, Zoran

Link to this page

Authority KeyName Variants
0500b8ac-70de-4bd7-ba7e-ef71d771d299
  • Zeković, Zoran (3)
Projects

Author's Bibliography

A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity

Zengin, Gökhan; Mollica, Adriano; Arsenijević, Jelena; Pavlić, Branimir; Zeković, Zoran; Sinan, Kouadio Ibrahime; Yan, Linlin; Cvetanović-Kljakić, Aleksandra; Ražić, Slavica

(MDPI, 2023)

TY  - JOUR
AU  - Zengin, Gökhan
AU  - Mollica, Adriano
AU  - Arsenijević, Jelena
AU  - Pavlić, Branimir
AU  - Zeković, Zoran
AU  - Sinan, Kouadio Ibrahime
AU  - Yan, Linlin
AU  - Cvetanović-Kljakić, Aleksandra
AU  - Ražić, Slavica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4418
AB  - Bearing in mind the centuries-old traditional use of chamomile, but also the increasing demand for its products in modern industry, oriented toward sustainable development, there are increasing efforts for the efficient extraction of high-value compounds of this plant, as well as obtaining its products with added value. With that goal, conventional and contemporary separation techniques were applied in this work. Both hydrodistillation processes (HD), conducted in a traditional manner and coupled with microwave irradiation (MWHD), were used for essential oil isolation. In parallel with those procedures, chamomile lipophilic extracts were obtained by Soxhlet extraction applying organic solvents and using supercritical fluid extraction as a greener approach. The obtained extracts and essential oils were characterized in terms of chemical composition (GC analysis, contents of total phenolics and flavonoids) and biological potential. GC analysis revealed that oxygenated sesquiterpenes and non-terpene compounds were the dominant compounds. α-Bisabolol oxide A (29.71–34.41%) and α-bisabolol oxide B (21.06–25.83%) were the most abundant individual components in samples obtained by distillation while in supercritical and Soxhlet extracts, major compounds were α-bisabolol oxide A and pentacosane. The biological potential of essential oils and extracts was tested by applying a set of analyzes to estimate the inhibition of biologically important enzymes (amylase, glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase) and antioxidant capacity (DPPH, ABTS, CUPRAC, FRAP, chelating and total antioxidant capacity). The results suggested essential oils as better antioxidants, while the extracts were proven to be better inhibitors of the tested enzymes. Principal Component Analysis was conducted using the experimental results of the composition of extracts and EOs of chamomile obtained by different separation techniques, showing clear discrimination between methods applied in correlation with the chemical profile. Molecular docking was applied for the identification of the main active principles present in the essential oil, among which α-bisabolol-oxide B (cp3) showed a higher affinity for tyrosinase.
PB  - MDPI
T2  - Separations
T1  - A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity
VL  - 10
IS  - 1
DO  - 10.3390/separations10010018
ER  - 
@article{
author = "Zengin, Gökhan and Mollica, Adriano and Arsenijević, Jelena and Pavlić, Branimir and Zeković, Zoran and Sinan, Kouadio Ibrahime and Yan, Linlin and Cvetanović-Kljakić, Aleksandra and Ražić, Slavica",
year = "2023",
abstract = "Bearing in mind the centuries-old traditional use of chamomile, but also the increasing demand for its products in modern industry, oriented toward sustainable development, there are increasing efforts for the efficient extraction of high-value compounds of this plant, as well as obtaining its products with added value. With that goal, conventional and contemporary separation techniques were applied in this work. Both hydrodistillation processes (HD), conducted in a traditional manner and coupled with microwave irradiation (MWHD), were used for essential oil isolation. In parallel with those procedures, chamomile lipophilic extracts were obtained by Soxhlet extraction applying organic solvents and using supercritical fluid extraction as a greener approach. The obtained extracts and essential oils were characterized in terms of chemical composition (GC analysis, contents of total phenolics and flavonoids) and biological potential. GC analysis revealed that oxygenated sesquiterpenes and non-terpene compounds were the dominant compounds. α-Bisabolol oxide A (29.71–34.41%) and α-bisabolol oxide B (21.06–25.83%) were the most abundant individual components in samples obtained by distillation while in supercritical and Soxhlet extracts, major compounds were α-bisabolol oxide A and pentacosane. The biological potential of essential oils and extracts was tested by applying a set of analyzes to estimate the inhibition of biologically important enzymes (amylase, glucosidase, acetylcholinesterase, butyrylcholinesterase, tyrosinase) and antioxidant capacity (DPPH, ABTS, CUPRAC, FRAP, chelating and total antioxidant capacity). The results suggested essential oils as better antioxidants, while the extracts were proven to be better inhibitors of the tested enzymes. Principal Component Analysis was conducted using the experimental results of the composition of extracts and EOs of chamomile obtained by different separation techniques, showing clear discrimination between methods applied in correlation with the chemical profile. Molecular docking was applied for the identification of the main active principles present in the essential oil, among which α-bisabolol-oxide B (cp3) showed a higher affinity for tyrosinase.",
publisher = "MDPI",
journal = "Separations",
title = "A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity",
volume = "10",
number = "1",
doi = "10.3390/separations10010018"
}
Zengin, G., Mollica, A., Arsenijević, J., Pavlić, B., Zeković, Z., Sinan, K. I., Yan, L., Cvetanović-Kljakić, A.,& Ražić, S.. (2023). A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity. in Separations
MDPI., 10(1).
https://doi.org/10.3390/separations10010018
Zengin G, Mollica A, Arsenijević J, Pavlić B, Zeković Z, Sinan KI, Yan L, Cvetanović-Kljakić A, Ražić S. A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity. in Separations. 2023;10(1).
doi:10.3390/separations10010018 .
Zengin, Gökhan, Mollica, Adriano, Arsenijević, Jelena, Pavlić, Branimir, Zeković, Zoran, Sinan, Kouadio Ibrahime, Yan, Linlin, Cvetanović-Kljakić, Aleksandra, Ražić, Slavica, "A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity" in Separations, 10, no. 1 (2023),
https://doi.org/10.3390/separations10010018 . .
2
8
7

Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower

Arsenijević, Jelena; Cvetanović, Aleksandra; Pavlović, Branimir; Ražić, Slavica; Maksimović, Zoran; Zeković, Zoran

(University of Niš, Serbia, 2018)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Cvetanović, Aleksandra
AU  - Pavlović, Branimir
AU  - Ražić, Slavica
AU  - Maksimović, Zoran
AU  - Zeković, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5271
AB  - Chamomile flower (Matricariae flos) is a herbal drug used mainly because of antiphlogistic, spasmolytic and antimicrobial properties, which are related to the presence of volatile essential-oil constituents (α-bisabolol and its oxides, chamazulene, spiroethers) and non-volatile compounds, such as sesquiterpene lactones, flavonoids, and coumarins.
In the present work, we used hydrodistillation (HD; sample-to-solvent ratio 1:20, 2 h), microwave-assisted HD (MHD; sample-to-solvent ratio 1:20, 2 h, microwave power 180 W) and supercritical CO2 extraction (SFE; 40 °C, 100 bar, 3 h) to obtain isolates, from a commercially available chamomile flower tea. The composition of the isolates was analyzed using GC-MS. The obtained results of the relative content of the selected pharmacologically relevant constituents were as follows.
HD and MHD yielded 0.2% and 0.3% of isolates, respectively. A much higher yield was obtained in the case of SFE (3%). In HD and MHD isolates, which were qualitatively and quantitatively similar, oxygenated sesquiterpenes dominated (64.4-67.4%), with bisabolol oxides comprising 54.2-54.4% of the isolates. Among non-terpene constituents (17.0-19.9%), spiroethers were present with 10.0% and 11.7% of HD and MHD isolates, respectively. Chamazulene (4.8-4.9%) was the most abundant among sesquiterpene hydrocarbons (8.8-9.7%). On the other hand, non-terpene compounds (57.4%) were the most abundant class of constituents in SFE extract, and among them hydrocarbons comprised 45.0% of the extract, followed by 10.2% of spiroethers. Among the oxygenated sesquiterpenes (32.2%), SFE extract contained 25.8% of bisabolol oxides. In contrast to HD and MHD, SFE resulted in a low amount of chamazulene, but the extract contained 3.2% of valuable sesquiterpene lactones.
Having in mind that HD and MHD resulted in similar yields and compositions of the isolates, and that SFE gave a higher amount of the isolate with a unique composition, the choice of traditional vs modern technique for the preparation of chamomile isolates should be strongly dependent on the specific use of the final product and in that sense carefully evaluated.
PB  - University of Niš, Serbia
C3  - Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
T1  - Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower
VL  - 16
IS  - 1 Special issue
SP  - 102
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5271
ER  - 
@conference{
author = "Arsenijević, Jelena and Cvetanović, Aleksandra and Pavlović, Branimir and Ražić, Slavica and Maksimović, Zoran and Zeković, Zoran",
year = "2018",
abstract = "Chamomile flower (Matricariae flos) is a herbal drug used mainly because of antiphlogistic, spasmolytic and antimicrobial properties, which are related to the presence of volatile essential-oil constituents (α-bisabolol and its oxides, chamazulene, spiroethers) and non-volatile compounds, such as sesquiterpene lactones, flavonoids, and coumarins.
In the present work, we used hydrodistillation (HD; sample-to-solvent ratio 1:20, 2 h), microwave-assisted HD (MHD; sample-to-solvent ratio 1:20, 2 h, microwave power 180 W) and supercritical CO2 extraction (SFE; 40 °C, 100 bar, 3 h) to obtain isolates, from a commercially available chamomile flower tea. The composition of the isolates was analyzed using GC-MS. The obtained results of the relative content of the selected pharmacologically relevant constituents were as follows.
HD and MHD yielded 0.2% and 0.3% of isolates, respectively. A much higher yield was obtained in the case of SFE (3%). In HD and MHD isolates, which were qualitatively and quantitatively similar, oxygenated sesquiterpenes dominated (64.4-67.4%), with bisabolol oxides comprising 54.2-54.4% of the isolates. Among non-terpene constituents (17.0-19.9%), spiroethers were present with 10.0% and 11.7% of HD and MHD isolates, respectively. Chamazulene (4.8-4.9%) was the most abundant among sesquiterpene hydrocarbons (8.8-9.7%). On the other hand, non-terpene compounds (57.4%) were the most abundant class of constituents in SFE extract, and among them hydrocarbons comprised 45.0% of the extract, followed by 10.2% of spiroethers. Among the oxygenated sesquiterpenes (32.2%), SFE extract contained 25.8% of bisabolol oxides. In contrast to HD and MHD, SFE resulted in a low amount of chamazulene, but the extract contained 3.2% of valuable sesquiterpene lactones.
Having in mind that HD and MHD resulted in similar yields and compositions of the isolates, and that SFE gave a higher amount of the isolate with a unique composition, the choice of traditional vs modern technique for the preparation of chamomile isolates should be strongly dependent on the specific use of the final product and in that sense carefully evaluated.",
publisher = "University of Niš, Serbia",
journal = "Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts",
title = "Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower",
volume = "16",
number = "1 Special issue",
pages = "102-102",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5271"
}
Arsenijević, J., Cvetanović, A., Pavlović, B., Ražić, S., Maksimović, Z.,& Zeković, Z.. (2018). Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts
University of Niš, Serbia., 16(1 Special issue), 102-102.
https://hdl.handle.net/21.15107/rcub_farfar_5271
Arsenijević J, Cvetanović A, Pavlović B, Ražić S, Maksimović Z, Zeković Z. Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower. in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts. 2018;16(1 Special issue):102-102.
https://hdl.handle.net/21.15107/rcub_farfar_5271 .
Arsenijević, Jelena, Cvetanović, Aleksandra, Pavlović, Branimir, Ražić, Slavica, Maksimović, Zoran, Zeković, Zoran, "Comparison of hydrodistillation (HD), microwave-assisted hydrodistillation (MHD) and supercritical fluid extraction (SFE) for the isolation of volatiles from chamomile flower" in Facta Universitatis, Series: Physics, Chemistry and Technology: 49th International Symposium on Essential Oils (ISEO2018), Book of Abstracts, 16, no. 1 Special issue (2018):102-102,
https://hdl.handle.net/21.15107/rcub_farfar_5271 .

Savremen pristup dobijanja funkcionalnih sastojaka kamilice

Cvetanović, Aleksandra; Pavlić, Branimir; Ražić, Slavica; Arsenijević, Jelena; Zengin, Gokhan; Uysal, Sengul; Zeković, Zoran

(Univerzitet u Beogradu, 2018)

TY  - CONF
AU  - Cvetanović, Aleksandra
AU  - Pavlić, Branimir
AU  - Ražić, Slavica
AU  - Arsenijević, Jelena
AU  - Zengin, Gokhan
AU  - Uysal, Sengul
AU  - Zeković, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5275
AB  - U okviru ovog rada cvast kamilice je ekstrahovana primenom dve različite tehnike, sa ciljem dobijanja proizvoda bogatog bioaktivnim jedinjenjima i funkcionalnim sastojcima. Za ovu svrhu, primenjena je jedna konvencionalna (Soxhlet extrakcija - SE) i jedna nekonvencionalna (superkritična ekstrakcija - SFE) tehnika. Detaljni hemijski sasatav SE i SFE ekstrakata je analiziran primenom GC - MS. Dodatno, izvršena je i procena biološke aktivnosti dobijenih ekstrakata. Prinos lipofine frakcije dobijene nakon tročasovne ekstrakcije čistim ugljen-dioksidom (350 bar i 40 °C) je bio približan prinosu ostvarenom SE ekstrakcijom u trajanju od 6 h pomoću metilen hlorida. Ispitivanje ekstarkata u smislu inhibicije acetilholinesteraze (AChE) i butirilholinesteraze (BChE) je pokazalo veći enzim-inhibitorni potencijala SFE uzorka. Imajući u vidu „zeleni" karakter i bezbednost SFE ekstrakata, prepoznat je veliki potencijal i mogućnost njihove primene kao sastojaka različitih prehrambenih funkcionalnih proizvoda, kao i brojnih farmaceutskih preparata.
AB  - In this study chamomile was extracted applying two different techniques in order to obtain the extract opulent with bioactive compounds. For this purpose one conventional (Soxhlet - SE) and one non-conventional (Supercritical fluid extraction - SFE) extraction technique were used. Detailed chemical composition of SE and SFE extracts was analysed by GC - MS. Additionally, biological activity of the chamomile products was examined as well. The yield of lipophilic fraction, achieved by 3h extraction, with CO2 at 350 bar and 40 °C was close to that one produced by Soxhlet performed for 6 h with methylene chloride. On the other hand, examination of their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) showed much higher enzyme-inhibitory potential of SFE extracts. Due to their green character and safety, SFE extracts have a great potential and possibilities for their use as ingredients in both numerous functional food products and pharmaceutical products, are recognized.
PB  - Univerzitet u Beogradu
C3  - UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova
T1  - Savremen pristup dobijanja funkcionalnih sastojaka kamilice
T1  - Emerging approach for the preparation of chamomile functional ingredients
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5275
ER  - 
@conference{
author = "Cvetanović, Aleksandra and Pavlić, Branimir and Ražić, Slavica and Arsenijević, Jelena and Zengin, Gokhan and Uysal, Sengul and Zeković, Zoran",
year = "2018",
abstract = "U okviru ovog rada cvast kamilice je ekstrahovana primenom dve različite tehnike, sa ciljem dobijanja proizvoda bogatog bioaktivnim jedinjenjima i funkcionalnim sastojcima. Za ovu svrhu, primenjena je jedna konvencionalna (Soxhlet extrakcija - SE) i jedna nekonvencionalna (superkritična ekstrakcija - SFE) tehnika. Detaljni hemijski sasatav SE i SFE ekstrakata je analiziran primenom GC - MS. Dodatno, izvršena je i procena biološke aktivnosti dobijenih ekstrakata. Prinos lipofine frakcije dobijene nakon tročasovne ekstrakcije čistim ugljen-dioksidom (350 bar i 40 °C) je bio približan prinosu ostvarenom SE ekstrakcijom u trajanju od 6 h pomoću metilen hlorida. Ispitivanje ekstarkata u smislu inhibicije acetilholinesteraze (AChE) i butirilholinesteraze (BChE) je pokazalo veći enzim-inhibitorni potencijala SFE uzorka. Imajući u vidu „zeleni" karakter i bezbednost SFE ekstrakata, prepoznat je veliki potencijal i mogućnost njihove primene kao sastojaka različitih prehrambenih funkcionalnih proizvoda, kao i brojnih farmaceutskih preparata., In this study chamomile was extracted applying two different techniques in order to obtain the extract opulent with bioactive compounds. For this purpose one conventional (Soxhlet - SE) and one non-conventional (Supercritical fluid extraction - SFE) extraction technique were used. Detailed chemical composition of SE and SFE extracts was analysed by GC - MS. Additionally, biological activity of the chamomile products was examined as well. The yield of lipophilic fraction, achieved by 3h extraction, with CO2 at 350 bar and 40 °C was close to that one produced by Soxhlet performed for 6 h with methylene chloride. On the other hand, examination of their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) showed much higher enzyme-inhibitory potential of SFE extracts. Due to their green character and safety, SFE extracts have a great potential and possibilities for their use as ingredients in both numerous functional food products and pharmaceutical products, are recognized.",
publisher = "Univerzitet u Beogradu",
journal = "UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova",
title = "Savremen pristup dobijanja funkcionalnih sastojaka kamilice, Emerging approach for the preparation of chamomile functional ingredients",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5275"
}
Cvetanović, A., Pavlić, B., Ražić, S., Arsenijević, J., Zengin, G., Uysal, S.,& Zeković, Z.. (2018). Savremen pristup dobijanja funkcionalnih sastojaka kamilice. in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova
Univerzitet u Beogradu..
https://hdl.handle.net/21.15107/rcub_farfar_5275
Cvetanović A, Pavlić B, Ražić S, Arsenijević J, Zengin G, Uysal S, Zeković Z. Savremen pristup dobijanja funkcionalnih sastojaka kamilice. in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova. 2018;.
https://hdl.handle.net/21.15107/rcub_farfar_5275 .
Cvetanović, Aleksandra, Pavlić, Branimir, Ražić, Slavica, Arsenijević, Jelena, Zengin, Gokhan, Uysal, Sengul, Zeković, Zoran, "Savremen pristup dobijanja funkcionalnih sastojaka kamilice" in UNIFOOD Konferencija, Beograd, 5-6 oktobar 2018, Program i zbornik radova (2018),
https://hdl.handle.net/21.15107/rcub_farfar_5275 .