TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Veljković, Milica
AU  - Stojanović, Nikola M.
AU  - Gocmanac-Ignjatović, Marija
AU  - Mihailov-Krstev, Tatjana
AU  - Branković, Suzana
AU  - Sokolović, Dušan
AU  - Marčetić, Mirjana
AU  - Radulović, Niko
AU  - Radenković, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2802
AB  - ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.
PB  - Wiley, Hoboken
T2  - Journal of Pharmacy and Pharmacology
T1  - Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties
VL  - 69
IS  - 9
SP  - 1208
EP  - 1218
DO  - 10.1111/jphp.12746
ER  - 

@article{
author = "Pavlović, Dragana R. and Veljković, Milica and Stojanović, Nikola M. and Gocmanac-Ignjatović, Marija and Mihailov-Krstev, Tatjana and Branković, Suzana and Sokolović, Dušan and Marčetić, Mirjana and Radulović, Niko and Radenković, Mirjana",
year = "2017",
abstract = "ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.",
publisher = "Wiley, Hoboken",
journal = "Journal of Pharmacy and Pharmacology",
title = "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties",
volume = "69",
number = "9",
pages = "1208-1218",
doi = "10.1111/jphp.12746"
}

Pavlović, D. R., Veljković, M., Stojanović, N. M., Gocmanac-Ignjatović, M., Mihailov-Krstev, T., Branković, S., Sokolović, D., Marčetić, M., Radulović, N.,& Radenković, M.. (2017). Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology
Wiley, Hoboken., 69(9), 1208-1218.
https://doi.org/10.1111/jphp.12746

Pavlović DR, Veljković M, Stojanović NM, Gocmanac-Ignjatović M, Mihailov-Krstev T, Branković S, Sokolović D, Marčetić M, Radulović N, Radenković M. Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology. 2017;69(9):1208-1218.
doi:10.1111/jphp.12746 .

Pavlović, Dragana R., Veljković, Milica, Stojanović, Nikola M., Gocmanac-Ignjatović, Marija, Mihailov-Krstev, Tatjana, Branković, Suzana, Sokolović, Dušan, Marčetić, Mirjana, Radulović, Niko, Radenković, Mirjana, "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties" in Journal of Pharmacy and Pharmacology, 69, no. 9 (2017):1208-1218,
https://doi.org/10.1111/jphp.12746 . .