TY  - JOUR
AU  - Mijušković, Mirjana
AU  - Stanojević, Ivan
AU  - Milović, Novak
AU  - Cerović, Snežana
AU  - Petrović, Dejan
AU  - Maksić, Đoko
AU  - Kovacević, Božidar
AU  - Anđelić, Tamara
AU  - Aleksić, Predrag
AU  - Terzić, Brankica
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3231
AB  - The objective of this prospective follow-up trial was to ascertain whether the urinary kidney injury molecule-1 (uKIM-1) associates with tumor tissue (tKIM-1) expression and with the pathological characteristics of clear renal cell carcinoma (cRCC) in radically nephrectomized (RN) and/or in partially nephrectomized (PN) patients with cRCC, pre- and postoperatively. This clinical study included 40 patients subjected to RN/PN (cRCC group) and 30 healthy volunteers (control group). Urinary KIM-1 was determined by ELISA TIM-1/KIM-1 kit and normalized by urinary creatinine. Immunohistochemical staining (monoclonal anti-human anti-TIM-1/KIM-1/HAVCR antibody) was used for semiquantitative analysis of the tKIM-1 expression and expressed as a score (% KIM-1 positively stained tubules). Both markers were interpreted in terms of the tumor characteristics comprising tumor size, Fuhrman grade, pathological (pT) stage, tumor/nodes/metastasis (TNM) stage, lymphovascular invasion and type of surgery RN/PN. Preoperative uKIM-1 was significantly higher in the cRCC group compared to controls, such as uKIM-1 was statistically higher in RN than in PN patients. Postoperatively, uKIM-1 decreased to control values. Expression of tKIM-1 was documented in all nephrectomized patients. Significant associations were achieved between uKIM-1 and tKIM-1 and with considered tumor characteristics, especially with tumor size and grade. Based on the accomplished associations, we found uKIM-1 as a highly sensitive marker for cRCC diagnosis. The clinical trial registration number: 1110-2012.
PB  - Springer, Dordrecht
T2  - International Urology and Nephrology
T1  - Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma
VL  - 50
IS  - 1
SP  - 63
EP  - 70
DO  - 10.1007/s11255-017-1724-6
ER  - 

@article{
author = "Mijušković, Mirjana and Stanojević, Ivan and Milović, Novak and Cerović, Snežana and Petrović, Dejan and Maksić, Đoko and Kovacević, Božidar and Anđelić, Tamara and Aleksić, Predrag and Terzić, Brankica and Đukić, Mirjana and Vojvodić, Danilo",
year = "2018",
abstract = "The objective of this prospective follow-up trial was to ascertain whether the urinary kidney injury molecule-1 (uKIM-1) associates with tumor tissue (tKIM-1) expression and with the pathological characteristics of clear renal cell carcinoma (cRCC) in radically nephrectomized (RN) and/or in partially nephrectomized (PN) patients with cRCC, pre- and postoperatively. This clinical study included 40 patients subjected to RN/PN (cRCC group) and 30 healthy volunteers (control group). Urinary KIM-1 was determined by ELISA TIM-1/KIM-1 kit and normalized by urinary creatinine. Immunohistochemical staining (monoclonal anti-human anti-TIM-1/KIM-1/HAVCR antibody) was used for semiquantitative analysis of the tKIM-1 expression and expressed as a score (% KIM-1 positively stained tubules). Both markers were interpreted in terms of the tumor characteristics comprising tumor size, Fuhrman grade, pathological (pT) stage, tumor/nodes/metastasis (TNM) stage, lymphovascular invasion and type of surgery RN/PN. Preoperative uKIM-1 was significantly higher in the cRCC group compared to controls, such as uKIM-1 was statistically higher in RN than in PN patients. Postoperatively, uKIM-1 decreased to control values. Expression of tKIM-1 was documented in all nephrectomized patients. Significant associations were achieved between uKIM-1 and tKIM-1 and with considered tumor characteristics, especially with tumor size and grade. Based on the accomplished associations, we found uKIM-1 as a highly sensitive marker for cRCC diagnosis. The clinical trial registration number: 1110-2012.",
publisher = "Springer, Dordrecht",
journal = "International Urology and Nephrology",
title = "Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma",
volume = "50",
number = "1",
pages = "63-70",
doi = "10.1007/s11255-017-1724-6"
}

Mijušković, M., Stanojević, I., Milović, N., Cerović, S., Petrović, D., Maksić, Đ., Kovacević, B., Anđelić, T., Aleksić, P., Terzić, B., Đukić, M.,& Vojvodić, D.. (2018). Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma. in International Urology and Nephrology
Springer, Dordrecht., 50(1), 63-70.
https://doi.org/10.1007/s11255-017-1724-6

Mijušković M, Stanojević I, Milović N, Cerović S, Petrović D, Maksić Đ, Kovacević B, Anđelić T, Aleksić P, Terzić B, Đukić M, Vojvodić D. Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma. in International Urology and Nephrology. 2018;50(1):63-70.
doi:10.1007/s11255-017-1724-6 .

Mijušković, Mirjana, Stanojević, Ivan, Milović, Novak, Cerović, Snežana, Petrović, Dejan, Maksić, Đoko, Kovacević, Božidar, Anđelić, Tamara, Aleksić, Predrag, Terzić, Brankica, Đukić, Mirjana, Vojvodić, Danilo, "Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma" in International Urology and Nephrology, 50, no. 1 (2018):63-70,
https://doi.org/10.1007/s11255-017-1724-6 . .

TY  - CONF
AU  - Mijušković, Mirjana
AU  - Stanojević, Ivan
AU  - Cerović, Snežana
AU  - Petrović, Dejan
AU  - Terzić, Brankica
AU  - Kovacević, Božidar
AU  - Anđelić, Tamara
AU  - Aleksić, Predrag
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2939
PB  - Oxford Univ Press, Oxford
C3  - Nephrology Dialysis Transplantation
T1  - Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma
VL  - 32
IS  - Supplement 3
SP  - iii438
EP  - iii438
DO  - 10.1093/ndt/gfx161.MP028
ER  - 

@conference{
author = "Mijušković, Mirjana and Stanojević, Ivan and Cerović, Snežana and Petrović, Dejan and Terzić, Brankica and Kovacević, Božidar and Anđelić, Tamara and Aleksić, Predrag and Đukić, Mirjana and Vojvodić, Danilo",
year = "2017",
publisher = "Oxford Univ Press, Oxford",
journal = "Nephrology Dialysis Transplantation",
title = "Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma",
volume = "32",
number = "Supplement 3",
pages = "iii438-iii438",
doi = "10.1093/ndt/gfx161.MP028"
}

Mijušković, M., Stanojević, I., Cerović, S., Petrović, D., Terzić, B., Kovacević, B., Anđelić, T., Aleksić, P., Đukić, M.,& Vojvodić, D.. (2017). Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma. in Nephrology Dialysis Transplantation
Oxford Univ Press, Oxford., 32(Supplement 3), iii438-iii438.
https://doi.org/10.1093/ndt/gfx161.MP028

Mijušković M, Stanojević I, Cerović S, Petrović D, Terzić B, Kovacević B, Anđelić T, Aleksić P, Đukić M, Vojvodić D. Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma. in Nephrology Dialysis Transplantation. 2017;32(Supplement 3):iii438-iii438.
doi:10.1093/ndt/gfx161.MP028 .

Mijušković, Mirjana, Stanojević, Ivan, Cerović, Snežana, Petrović, Dejan, Terzić, Brankica, Kovacević, Božidar, Anđelić, Tamara, Aleksić, Predrag, Đukić, Mirjana, Vojvodić, Danilo, "Tissue and urinary kim-1 relate with tumor characteristics in patients with clear renal cell carcinoma" in Nephrology Dialysis Transplantation, 32, no. Supplement 3 (2017):iii438-iii438,
https://doi.org/10.1093/ndt/gfx161.MP028 . .

TY  - JOUR
AU  - Mijušković, Mirjana
AU  - Stanojević, Ivan
AU  - Milović, Novak
AU  - Cerović, Snežana
AU  - Petrović, Dejan
AU  - Jovanović, Dragan
AU  - Aleksić, Predrag
AU  - Kovačević, Božidar
AU  - Anđelić, Tamara
AU  - Terzić, Brankica
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2676
AB  - Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The aim of this study was to ascertain relationship between the urine concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of operation (radical or partial nephrectomy) in patients with cRCC. Methods. Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1) were determined by commercially available ELISA kits. The analysis included 40 patients undergoing partial or radical nephrectomy for cRCC and 40 age- and sex-matched healthy adult volunteers. Results. The median preoperative concentrations of KIM-1 in the cRCC group [0.724 ± 1.120 ng/mg urinary creatinine (Ucr)] were significantly greater compared with controls (healthy volunteers) (0.210 ± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1 concentration decreased significantly to control values (0.177 ± 0.099 ng/mgUcr vs 0.210 ± 0.082 ng/mgUcr, respectively). The size, grade and stage of tumor were correlated positively with preoperative uKIM-1 concentrations. Contrary to these results, concentrations of uAQP-1 in the cRCC group were significantly lower (0.111 ± 0.092 ng/mgUcr) compared with the control group (0.202 ± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations of uAQP-1 increased progressively up to control values, approximately. We find no significant correlation between preoperative uAQP-1 concentrations and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a reliable diagnostic marker of cRCC, based on its significantly increased values before and decreased values after the nephrectomy.
AB  - Uvod/Cilj. Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) su potencijalni rani biomarkeri karcinoma svetlih ćelija (cRCC). Cilj ove studije bio je da se utvrdi povezanost između koncentracija KIM-1 i AQP-1 u urinu i veličine, gradusa, stadijuma i vrste operacije (radikalna ili parcijalna nefrektomija) kod bolesnika sa cRCC. Metode. Urinarne koncentracije KIM-1 i AQP-1 određene su primenom komercijalnih ELISA kitova. Analizom je bilo obuhvaćeno 40 bolesnika koji su bili podvrgnuti parcijalnoj ili radikalnoj nefrektomiji zbog tumora bubrega i 40 zdravih odraslih ispitanika. Grupe su bile komparabilne po polu i godinama života. Rezultati. Srednja preoperativna koncentracija urinarnog KIM-1 (uKIM-1) u cRCC grupi [0,724 ± 1,120 ng/mg kreatinia u urinu (Ucr)] bila je statistički značajno viša u poređenju sa koncentracijom u kontrolnoj grupi (0,210 ± 0,082 ng/mgUcr ) (p = 0,0227). Postoperativno, koncentracija uKIM-1 značajno je padala i približavala se vrednosti u kontrolnoj grupi (0,177 ± 0,099 ng/mgUcr nasuprot 0,210 ± 0,082 ng/mgUcr). Veličina, gradus i stadijum tumora bili su u pozitivnoj korelaciji sa preoperativnim koncentracijama uKIM-1. Nasuprot ovim rezultatima, koncentracija urinarnog AQP-1 (uAQP-1) u cRCC grupi bila je značajno niža (0,111 ± 0,092 ng/mgUcr) u poređenju sa kontrolnom grupom zdravih osoba (0,202 ± 0,078 ng/mgUcr) (p = 0,0014). Postoperativno, koncentracija uAQP-1 progresivno se povećavala, približno do vrednosti u kontrolnoj grupi. Nismo našli značajnu korelaciju između preoperativnih koncentracija uAQP-1 i veličine, gradusa i stadijuma tumora. Zaključak. uKIM-1 bi mogao biti dodatni pouzdani dijagnostički marker za cRRC na osnovu njegove značajno više preoperativne koncentracije i sniženja vrednosti nakon nefrektomije.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers
T1  - Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) u urinu kod bolesnika sa karcinomom svetlih ćelija bubrega - potencijalni neiznvazivni biomarkeri
VL  - 73
IS  - 3
SP  - 266
EP  - 272
DO  - 10.2298/VSP150124006M
ER  - 

@article{
author = "Mijušković, Mirjana and Stanojević, Ivan and Milović, Novak and Cerović, Snežana and Petrović, Dejan and Jovanović, Dragan and Aleksić, Predrag and Kovačević, Božidar and Anđelić, Tamara and Terzić, Brankica and Đukić, Mirjana and Vojvodić, Danilo",
year = "2016",
abstract = "Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The aim of this study was to ascertain relationship between the urine concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of operation (radical or partial nephrectomy) in patients with cRCC. Methods. Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1) were determined by commercially available ELISA kits. The analysis included 40 patients undergoing partial or radical nephrectomy for cRCC and 40 age- and sex-matched healthy adult volunteers. Results. The median preoperative concentrations of KIM-1 in the cRCC group [0.724 ± 1.120 ng/mg urinary creatinine (Ucr)] were significantly greater compared with controls (healthy volunteers) (0.210 ± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1 concentration decreased significantly to control values (0.177 ± 0.099 ng/mgUcr vs 0.210 ± 0.082 ng/mgUcr, respectively). The size, grade and stage of tumor were correlated positively with preoperative uKIM-1 concentrations. Contrary to these results, concentrations of uAQP-1 in the cRCC group were significantly lower (0.111 ± 0.092 ng/mgUcr) compared with the control group (0.202 ± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations of uAQP-1 increased progressively up to control values, approximately. We find no significant correlation between preoperative uAQP-1 concentrations and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a reliable diagnostic marker of cRCC, based on its significantly increased values before and decreased values after the nephrectomy., Uvod/Cilj. Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) su potencijalni rani biomarkeri karcinoma svetlih ćelija (cRCC). Cilj ove studije bio je da se utvrdi povezanost između koncentracija KIM-1 i AQP-1 u urinu i veličine, gradusa, stadijuma i vrste operacije (radikalna ili parcijalna nefrektomija) kod bolesnika sa cRCC. Metode. Urinarne koncentracije KIM-1 i AQP-1 određene su primenom komercijalnih ELISA kitova. Analizom je bilo obuhvaćeno 40 bolesnika koji su bili podvrgnuti parcijalnoj ili radikalnoj nefrektomiji zbog tumora bubrega i 40 zdravih odraslih ispitanika. Grupe su bile komparabilne po polu i godinama života. Rezultati. Srednja preoperativna koncentracija urinarnog KIM-1 (uKIM-1) u cRCC grupi [0,724 ± 1,120 ng/mg kreatinia u urinu (Ucr)] bila je statistički značajno viša u poređenju sa koncentracijom u kontrolnoj grupi (0,210 ± 0,082 ng/mgUcr ) (p = 0,0227). Postoperativno, koncentracija uKIM-1 značajno je padala i približavala se vrednosti u kontrolnoj grupi (0,177 ± 0,099 ng/mgUcr nasuprot 0,210 ± 0,082 ng/mgUcr). Veličina, gradus i stadijum tumora bili su u pozitivnoj korelaciji sa preoperativnim koncentracijama uKIM-1. Nasuprot ovim rezultatima, koncentracija urinarnog AQP-1 (uAQP-1) u cRCC grupi bila je značajno niža (0,111 ± 0,092 ng/mgUcr) u poređenju sa kontrolnom grupom zdravih osoba (0,202 ± 0,078 ng/mgUcr) (p = 0,0014). Postoperativno, koncentracija uAQP-1 progresivno se povećavala, približno do vrednosti u kontrolnoj grupi. Nismo našli značajnu korelaciju između preoperativnih koncentracija uAQP-1 i veličine, gradusa i stadijuma tumora. Zaključak. uKIM-1 bi mogao biti dodatni pouzdani dijagnostički marker za cRRC na osnovu njegove značajno više preoperativne koncentracije i sniženja vrednosti nakon nefrektomije.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers, Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) u urinu kod bolesnika sa karcinomom svetlih ćelija bubrega - potencijalni neiznvazivni biomarkeri",
volume = "73",
number = "3",
pages = "266-272",
doi = "10.2298/VSP150124006M"
}

Mijušković, M., Stanojević, I., Milović, N., Cerović, S., Petrović, D., Jovanović, D., Aleksić, P., Kovačević, B., Anđelić, T., Terzić, B., Đukić, M.,& Vojvodić, D.. (2016). Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(3), 266-272.
https://doi.org/10.2298/VSP150124006M

Mijušković M, Stanojević I, Milović N, Cerović S, Petrović D, Jovanović D, Aleksić P, Kovačević B, Anđelić T, Terzić B, Đukić M, Vojvodić D. Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers. in Vojnosanitetski pregled. 2016;73(3):266-272.
doi:10.2298/VSP150124006M .

Mijušković, Mirjana, Stanojević, Ivan, Milović, Novak, Cerović, Snežana, Petrović, Dejan, Jovanović, Dragan, Aleksić, Predrag, Kovačević, Božidar, Anđelić, Tamara, Terzić, Brankica, Đukić, Mirjana, Vojvodić, Danilo, "Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers" in Vojnosanitetski pregled, 73, no. 3 (2016):266-272,
https://doi.org/10.2298/VSP150124006M . .