TY  - JOUR
AU  - Đorđević, S
AU  - Kovacević, I
AU  - Miljković, Branislava
AU  - Vuksanović, J
AU  - Pokrajac, Milena
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/641
AB  - A selective, sensitive, simple, and rapid method for the simultaneous determination of fluoxetine (FL) and norfluoxetine (nor-FL) was developed and validated, and further applied to analyze plasma samples obtained from FL-treated patients with Parkinson disease (n:=:18). After one step liquid-liquid extraction with ethyl acetate, plasma samples were chromatographed on a C8 column. The mobile phase was acetate buffer and acetonitrile (40:60 v/v). Determination of FL and nor-FL was performed with MS detection in selective ion monitoring (SIM) mode, so the other components did not interfere with this assay. FL, nor-FL and flumazenil as internal standard were eluted in 6:min. Recoveries ranged from 89.7 to 96.6% and from 80.2 to 85.3% for FL and nor-FL, respectively. The limit of quantitation under described conditions was 2,5 μg/l for FL and 10 μg/l for nor-FL. The method was found to be reproducible with coefficient of variation less than 9%. The parameters of the method were found to be acceptable to enable its routine use for clinical studies. The method was employed to analyze the Parkinsonian patients' plasma samples. A great deviation in plasma concentrations of FL and nor-FL found among 18 studied patients indicates high pharmacokinetic variability of the drug. Obtained results also indicate absence of the influence of Parkinson disease on the drug disposition.
PB  - Elsevier Masson SAS
T2  - Farmaco
T1  - Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study
VL  - 60
IS  - 4
SP  - 345
EP  - 349
DO  - 10.1016/j.farmac.2005.01.002
ER  - 

@article{
author = "Đorđević, S and Kovacević, I and Miljković, Branislava and Vuksanović, J and Pokrajac, Milena",
year = "2005",
abstract = "A selective, sensitive, simple, and rapid method for the simultaneous determination of fluoxetine (FL) and norfluoxetine (nor-FL) was developed and validated, and further applied to analyze plasma samples obtained from FL-treated patients with Parkinson disease (n:=:18). After one step liquid-liquid extraction with ethyl acetate, plasma samples were chromatographed on a C8 column. The mobile phase was acetate buffer and acetonitrile (40:60 v/v). Determination of FL and nor-FL was performed with MS detection in selective ion monitoring (SIM) mode, so the other components did not interfere with this assay. FL, nor-FL and flumazenil as internal standard were eluted in 6:min. Recoveries ranged from 89.7 to 96.6% and from 80.2 to 85.3% for FL and nor-FL, respectively. The limit of quantitation under described conditions was 2,5 μg/l for FL and 10 μg/l for nor-FL. The method was found to be reproducible with coefficient of variation less than 9%. The parameters of the method were found to be acceptable to enable its routine use for clinical studies. The method was employed to analyze the Parkinsonian patients' plasma samples. A great deviation in plasma concentrations of FL and nor-FL found among 18 studied patients indicates high pharmacokinetic variability of the drug. Obtained results also indicate absence of the influence of Parkinson disease on the drug disposition.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study",
volume = "60",
number = "4",
pages = "345-349",
doi = "10.1016/j.farmac.2005.01.002"
}

Đorđević, S., Kovacević, I., Miljković, B., Vuksanović, J.,& Pokrajac, M.. (2005). Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study. in Farmaco
Elsevier Masson SAS., 60(4), 345-349.
https://doi.org/10.1016/j.farmac.2005.01.002

Đorđević S, Kovacević I, Miljković B, Vuksanović J, Pokrajac M. Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study. in Farmaco. 2005;60(4):345-349.
doi:10.1016/j.farmac.2005.01.002 .

Đorđević, S, Kovacević, I, Miljković, Branislava, Vuksanović, J, Pokrajac, Milena, "Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study" in Farmaco, 60, no. 4 (2005):345-349,
https://doi.org/10.1016/j.farmac.2005.01.002 . .