TY  - JOUR
AU  - Colić, M.
AU  - Došlov-Kokoruš, Zvezdana
AU  - Backović, A.
AU  - Stojanović, D.
AU  - Antić-Stanković, Jelena
AU  - Kovačević, Nada
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/989
AB  - The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo
VL  - 62
IS  - 9
SP  - 705
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_farfar_989
ER  - 

@article{
author = "Colić, M. and Došlov-Kokoruš, Zvezdana and Backović, A. and Stojanović, D. and Antić-Stanković, Jelena and Kovačević, Nada",
year = "2007",
abstract = "The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo",
volume = "62",
number = "9",
pages = "705-708",
url = "https://hdl.handle.net/21.15107/rcub_farfar_989"
}

Colić, M., Došlov-Kokoruš, Z., Backović, A., Stojanović, D., Antić-Stanković, J.,& Kovačević, N.. (2007). Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 62(9), 705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989

Colić M, Došlov-Kokoruš Z, Backović A, Stojanović D, Antić-Stanković J, Kovačević N. Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie. 2007;62(9):705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989 .

Colić, M., Došlov-Kokoruš, Zvezdana, Backović, A., Stojanović, D., Antić-Stanković, Jelena, Kovačević, Nada, "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo" in Pharmazie, 62, no. 9 (2007):705-708,
https://hdl.handle.net/21.15107/rcub_farfar_989 .

TY  - JOUR
AU  - Colić, M
AU  - Stojić-Vukanić, Zorica
AU  - Pavlović, B
AU  - Jandrić, Dušan
AU  - Stefanoska, I
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/433
AB  - We have studied the effect of mycophenolate mofetil (MMF), a new drug used in prevention of transplant rejection, on differentiation, maturation and allostimulatory activity of human monocyte-derived dendritic cells (MDDC). MDDC were generated in vitro with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4 in the presence or absence of MMF. MMF reduced the number of immature MDDC in culture, dose-dependently, by inducing apoptosis and inhibited their stimulatory activity on allogeneic lymphocytes. These changes correlated with down-regulation of costimulatory and adhesion molecules such as CD40, CD54, CD80 and CD86. No differences were observed in mannose receptor (MR)-mediated endocytosis, measured by the uptake of fluorescein isothiocyanate (FITC)-dextran. MDDC differentiated in the presence of MMF showed significantly reduced maturation upon stimulation with lipopolysaccharide, as judged by lower expresson of CD83 and co-stimulatory molecules, lower production of tumour necrosis factor (TNF)-alpha, IL-10, IL-12 and IL-18 as well as lower stimulation of alloreactive T cells including naive CD4(+) CD45RA(+)T cells. In contrast, MDDC matured in the presence of MMF showed a more marked decrease in the FITC-dextran uptake than mature MDDC cultivated without MMF and the phenomenon correlated with down-regulation of the MR expression. These results suggest that MMF impairs differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.
PB  - Blackwell Publishing Ltd, Oxford
T2  - Clinical and Experimental Immunology
T1  - Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells
VL  - 134
IS  - 1
SP  - 63
EP  - 69
DO  - 10.1046/j.1365-2249.2003.02269.x
ER  - 

@article{
author = "Colić, M and Stojić-Vukanić, Zorica and Pavlović, B and Jandrić, Dušan and Stefanoska, I",
year = "2003",
abstract = "We have studied the effect of mycophenolate mofetil (MMF), a new drug used in prevention of transplant rejection, on differentiation, maturation and allostimulatory activity of human monocyte-derived dendritic cells (MDDC). MDDC were generated in vitro with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4 in the presence or absence of MMF. MMF reduced the number of immature MDDC in culture, dose-dependently, by inducing apoptosis and inhibited their stimulatory activity on allogeneic lymphocytes. These changes correlated with down-regulation of costimulatory and adhesion molecules such as CD40, CD54, CD80 and CD86. No differences were observed in mannose receptor (MR)-mediated endocytosis, measured by the uptake of fluorescein isothiocyanate (FITC)-dextran. MDDC differentiated in the presence of MMF showed significantly reduced maturation upon stimulation with lipopolysaccharide, as judged by lower expresson of CD83 and co-stimulatory molecules, lower production of tumour necrosis factor (TNF)-alpha, IL-10, IL-12 and IL-18 as well as lower stimulation of alloreactive T cells including naive CD4(+) CD45RA(+)T cells. In contrast, MDDC matured in the presence of MMF showed a more marked decrease in the FITC-dextran uptake than mature MDDC cultivated without MMF and the phenomenon correlated with down-regulation of the MR expression. These results suggest that MMF impairs differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.",
publisher = "Blackwell Publishing Ltd, Oxford",
journal = "Clinical and Experimental Immunology",
title = "Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells",
volume = "134",
number = "1",
pages = "63-69",
doi = "10.1046/j.1365-2249.2003.02269.x"
}

Colić, M., Stojić-Vukanić, Z., Pavlović, B., Jandrić, D.,& Stefanoska, I.. (2003). Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells. in Clinical and Experimental Immunology
Blackwell Publishing Ltd, Oxford., 134(1), 63-69.
https://doi.org/10.1046/j.1365-2249.2003.02269.x

Colić M, Stojić-Vukanić Z, Pavlović B, Jandrić D, Stefanoska I. Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells. in Clinical and Experimental Immunology. 2003;134(1):63-69.
doi:10.1046/j.1365-2249.2003.02269.x .

Colić, M, Stojić-Vukanić, Zorica, Pavlović, B, Jandrić, Dušan, Stefanoska, I, "Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells" in Clinical and Experimental Immunology, 134, no. 1 (2003):63-69,
https://doi.org/10.1046/j.1365-2249.2003.02269.x . .

TY  - JOUR
AU  - Zunić, G
AU  - Jelić-Ivanović, Zorana
AU  - Colić, M
AU  - Spasić, S
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/328
AB  - This report describes a rapid, single-run procedure, based on the optimization of capillary electrophoresis (CE) and indirect absorbance detection capabilities, which was developed for the separation and quantification of 30 underivatized physiological amino acids and peptides, usually present in biological fluids. p-Aminosalicylic acid buffered with sodium carbonate at pH 10.2 +/- 0.1 was used as the running electrolyte. Electrophoresis, carried out in a capillary (87 cmx75 mum) at 15 kV potential (normal polarity), separated the examined compounds within 30 min. Limits of detection ranged from 1.93 to 20.08 mumol/l (median 6.71 mumol/l). The method was linear within the 50-200 mumol/l concentration range (r ranged from 0.684 to 0.989, median r=0.934). Within run migration times precision was good (median C.V.=0.7%). Less favorable within run peak area precision (median C.V.=6.6%) was obtained. The analytical procedure presented was successfully tested for separation and quantification of amino acids in physiological fluids, such as plasma or supernatant of macrophage cultures. Sample preparations require only a protein precipitation and dilution step.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids
VL  - 772
IS  - 1
SP  - 19
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_farfar_328
ER  - 

@article{
author = "Zunić, G and Jelić-Ivanović, Zorana and Colić, M and Spasić, S",
year = "2002",
abstract = "This report describes a rapid, single-run procedure, based on the optimization of capillary electrophoresis (CE) and indirect absorbance detection capabilities, which was developed for the separation and quantification of 30 underivatized physiological amino acids and peptides, usually present in biological fluids. p-Aminosalicylic acid buffered with sodium carbonate at pH 10.2 +/- 0.1 was used as the running electrolyte. Electrophoresis, carried out in a capillary (87 cmx75 mum) at 15 kV potential (normal polarity), separated the examined compounds within 30 min. Limits of detection ranged from 1.93 to 20.08 mumol/l (median 6.71 mumol/l). The method was linear within the 50-200 mumol/l concentration range (r ranged from 0.684 to 0.989, median r=0.934). Within run migration times precision was good (median C.V.=0.7%). Less favorable within run peak area precision (median C.V.=6.6%) was obtained. The analytical procedure presented was successfully tested for separation and quantification of amino acids in physiological fluids, such as plasma or supernatant of macrophage cultures. Sample preparations require only a protein precipitation and dilution step.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids",
volume = "772",
number = "1",
pages = "19-33",
url = "https://hdl.handle.net/21.15107/rcub_farfar_328"
}

Zunić, G., Jelić-Ivanović, Z., Colić, M.,& Spasić, S.. (2002). Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 772(1), 19-33.
https://hdl.handle.net/21.15107/rcub_farfar_328

Zunić G, Jelić-Ivanović Z, Colić M, Spasić S. Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2002;772(1):19-33.
https://hdl.handle.net/21.15107/rcub_farfar_328 .

Zunić, G, Jelić-Ivanović, Zorana, Colić, M, Spasić, S, "Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 772, no. 1 (2002):19-33,
https://hdl.handle.net/21.15107/rcub_farfar_328 .

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Dimitrijević, M
AU  - Colić, M
AU  - Popović, P
AU  - Jandrić, Dušan
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/287
PB  - Elsevier Science Inc, New York
T2  - Turkish Journal of Medical Sciences
T1  - Modulation of human peripheral blood mononuclear cell activation by the combination of leflunomide and pentoxifylline
VL  - 33
IS  - 3
SP  - 2137
EP  - 2138
DO  - 10.1016/S0041-1345(01)01975-3
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Dimitrijević, M and Colić, M and Popović, P and Jandrić, Dušan",
year = "2001",
publisher = "Elsevier Science Inc, New York",
journal = "Turkish Journal of Medical Sciences",
title = "Modulation of human peripheral blood mononuclear cell activation by the combination of leflunomide and pentoxifylline",
volume = "33",
number = "3",
pages = "2137-2138",
doi = "10.1016/S0041-1345(01)01975-3"
}

Stojić-Vukanić, Z., Dimitrijević, M., Colić, M., Popović, P.,& Jandrić, D.. (2001). Modulation of human peripheral blood mononuclear cell activation by the combination of leflunomide and pentoxifylline. in Turkish Journal of Medical Sciences
Elsevier Science Inc, New York., 33(3), 2137-2138.
https://doi.org/10.1016/S0041-1345(01)01975-3

Stojić-Vukanić Z, Dimitrijević M, Colić M, Popović P, Jandrić D. Modulation of human peripheral blood mononuclear cell activation by the combination of leflunomide and pentoxifylline. in Turkish Journal of Medical Sciences. 2001;33(3):2137-2138.
doi:10.1016/S0041-1345(01)01975-3 .

Stojić-Vukanić, Zorica, Dimitrijević, M, Colić, M, Popović, P, Jandrić, Dušan, "Modulation of human peripheral blood mononuclear cell activation by the combination of leflunomide and pentoxifylline" in Turkish Journal of Medical Sciences, 33, no. 3 (2001):2137-2138,
https://doi.org/10.1016/S0041-1345(01)01975-3 . .

TY  - JOUR
AU  - Arsenović-Ranin, Nevena
AU  - Vucević, D
AU  - Okada, N
AU  - Dimitrijević, M
AU  - Colić, M
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/244
AB  - A murine monoclonal antibody (mAb), 3F10, was produced by fusion of spleen cells obtained from mice immunized with a rat cortical thymic epithelial cell line (R-TNC.1) stimulated with interferon-gamma and P3X myeloma cells. 3F10 recognized an antigen expressed both on thymocytes and non-lymphoid cells in the thymus. Flow cytometry showed that 3F10 stained more than 98% thymocytes and 90% R-TNC.1 cells. Immunoprecipitation and Western blot studies demonstrated that 3F10 reacted with molecules of 55000 and 65000 MW from both thymocyte and R-TNC.1 cell lysates. 3F10 recognized the same antigen on Chinese hamster ovary cells transfected with rat Crry as did 5I2 mAb, confirming the specificity of 3F10 mAb for the rat homologue of mouse Crry/p65, a membrane-bound complement regulatory protein. 3F10 mAb induced homotypic aggregation of thymocytes and exhibited an additive effect on the aggregation evoked by phorbol myristate acetate. The aggregation was dependent on active cell metabolism, intact cytoskeleton, divalent cations and activation of protein phosphatases 1 and 2A (as assessed by use of okadaic acid). In contrast, H-7, HA1004 and genistein partially inhibited, whereas staurosporine potentiated the aggregation of thymocytes triggered by 3F10. 3F10 mAb also stimulated binding of thymocytes to the R-TNC.1 line. Both homotypic and heterotypic adhesive interactions are mediated by leucocyte function-associated antigen-1 (LFA-1). In addition, 3F10 stimulated proliferation of thymocytes induced by suboptimal concentrations of concanavalin A. These data suggest that rat Crry/p65 might be involved in the regulation of both cell adhesion and activation of thymocytes. This is a novel, non-complement-dependent function of Crry/p65.
PB  - Blackwell Science Ltd, Oxford
T2  - Immunology
T1  - A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes
VL  - 100
IS  - 3
SP  - 334
EP  - 344
DO  - 10.1046/j.1365-2567.2000.00043.x
ER  - 

@article{
author = "Arsenović-Ranin, Nevena and Vucević, D and Okada, N and Dimitrijević, M and Colić, M",
year = "2000",
abstract = "A murine monoclonal antibody (mAb), 3F10, was produced by fusion of spleen cells obtained from mice immunized with a rat cortical thymic epithelial cell line (R-TNC.1) stimulated with interferon-gamma and P3X myeloma cells. 3F10 recognized an antigen expressed both on thymocytes and non-lymphoid cells in the thymus. Flow cytometry showed that 3F10 stained more than 98% thymocytes and 90% R-TNC.1 cells. Immunoprecipitation and Western blot studies demonstrated that 3F10 reacted with molecules of 55000 and 65000 MW from both thymocyte and R-TNC.1 cell lysates. 3F10 recognized the same antigen on Chinese hamster ovary cells transfected with rat Crry as did 5I2 mAb, confirming the specificity of 3F10 mAb for the rat homologue of mouse Crry/p65, a membrane-bound complement regulatory protein. 3F10 mAb induced homotypic aggregation of thymocytes and exhibited an additive effect on the aggregation evoked by phorbol myristate acetate. The aggregation was dependent on active cell metabolism, intact cytoskeleton, divalent cations and activation of protein phosphatases 1 and 2A (as assessed by use of okadaic acid). In contrast, H-7, HA1004 and genistein partially inhibited, whereas staurosporine potentiated the aggregation of thymocytes triggered by 3F10. 3F10 mAb also stimulated binding of thymocytes to the R-TNC.1 line. Both homotypic and heterotypic adhesive interactions are mediated by leucocyte function-associated antigen-1 (LFA-1). In addition, 3F10 stimulated proliferation of thymocytes induced by suboptimal concentrations of concanavalin A. These data suggest that rat Crry/p65 might be involved in the regulation of both cell adhesion and activation of thymocytes. This is a novel, non-complement-dependent function of Crry/p65.",
publisher = "Blackwell Science Ltd, Oxford",
journal = "Immunology",
title = "A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes",
volume = "100",
number = "3",
pages = "334-344",
doi = "10.1046/j.1365-2567.2000.00043.x"
}

Arsenović-Ranin, N., Vucević, D., Okada, N., Dimitrijević, M.,& Colić, M.. (2000). A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes. in Immunology
Blackwell Science Ltd, Oxford., 100(3), 334-344.
https://doi.org/10.1046/j.1365-2567.2000.00043.x

Arsenović-Ranin N, Vucević D, Okada N, Dimitrijević M, Colić M. A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes. in Immunology. 2000;100(3):334-344.
doi:10.1046/j.1365-2567.2000.00043.x .

Arsenović-Ranin, Nevena, Vucević, D, Okada, N, Dimitrijević, M, Colić, M, "A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes" in Immunology, 100, no. 3 (2000):334-344,
https://doi.org/10.1046/j.1365-2567.2000.00043.x . .

TY  - JOUR
AU  - Dimitrijević, Miroslava
AU  - Milenković, Marina
AU  - Milosavljević, P
AU  - Stojić-Vukanić, Zorica
AU  - Colić, M
AU  - Bartlett, R
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/186
AB  - The protective and therapeutical potential of a novel immunomodulatory agent, leflunomide, has been demonstrated in several experimental models of autoimmunity reactions leading to transplant rejection, as well as the therapy of patients with rheumatoid arthritis. in this study, the effects of leflunomide on experimentally induced autoimmune myocarditis (EAM) were investigated. Genetically susceptible DA rats were immunized with porcine cardiac myosin in complete Freund's adjuvant. The course of the disease was examined (on day 8, 16, 21 and 34) by macroscopic and microscopic scoring, heart weight/body weight ratio was determined and immunohistochemical analysis of inflammatory cell infiltrates was conducted. Eight days after disease induction, only slightly elevated expression of adhesive molecules on the interstitial and vascular endothelial cells were observed otherwise no microscopic signs of the inflammation existed. On day 16, numerous inflammatory cells infiltrated the myocardium. By day 21, the severity of myocarditis was substantially increased and was accompanied by extensive necrosis. After 34 days, decreased number of infiltrated cells was detected together with myocardial necrosis. Effects of leflunomide were evaluated by two treatment protocols. Rats immunized with cardiac myosin were injected i.p. with leflunomide's active metabolite, A 771726, at a dose of 10 mg/kg/day either during the first 6 days, or starting from day 14 and ending a: day 20 after disease induction. Efficacy of leflunomide treatment was determined on day 21 of EAM development Results demonstrated that early leflunomide treatment inhibited the inflammatory reaction, while late treatment significantly reduced EAM progression. Leflunomide may be considered a potent therapeutical tool for autoimmune myocarditis.
PB  - Bioscience Ediprint Inc, Carouge
T2  - International Journal of Immunotherapy
T1  - Beneficial effects of leflunomide on cardiac myosin-induced experimental autoimmune myocarditis in rats
VL  - 14
IS  - 1
SP  - 9
EP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_farfar_186
ER  - 

@article{
author = "Dimitrijević, Miroslava and Milenković, Marina and Milosavljević, P and Stojić-Vukanić, Zorica and Colić, M and Bartlett, R",
year = "1998",
abstract = "The protective and therapeutical potential of a novel immunomodulatory agent, leflunomide, has been demonstrated in several experimental models of autoimmunity reactions leading to transplant rejection, as well as the therapy of patients with rheumatoid arthritis. in this study, the effects of leflunomide on experimentally induced autoimmune myocarditis (EAM) were investigated. Genetically susceptible DA rats were immunized with porcine cardiac myosin in complete Freund's adjuvant. The course of the disease was examined (on day 8, 16, 21 and 34) by macroscopic and microscopic scoring, heart weight/body weight ratio was determined and immunohistochemical analysis of inflammatory cell infiltrates was conducted. Eight days after disease induction, only slightly elevated expression of adhesive molecules on the interstitial and vascular endothelial cells were observed otherwise no microscopic signs of the inflammation existed. On day 16, numerous inflammatory cells infiltrated the myocardium. By day 21, the severity of myocarditis was substantially increased and was accompanied by extensive necrosis. After 34 days, decreased number of infiltrated cells was detected together with myocardial necrosis. Effects of leflunomide were evaluated by two treatment protocols. Rats immunized with cardiac myosin were injected i.p. with leflunomide's active metabolite, A 771726, at a dose of 10 mg/kg/day either during the first 6 days, or starting from day 14 and ending a: day 20 after disease induction. Efficacy of leflunomide treatment was determined on day 21 of EAM development Results demonstrated that early leflunomide treatment inhibited the inflammatory reaction, while late treatment significantly reduced EAM progression. Leflunomide may be considered a potent therapeutical tool for autoimmune myocarditis.",
publisher = "Bioscience Ediprint Inc, Carouge",
journal = "International Journal of Immunotherapy",
title = "Beneficial effects of leflunomide on cardiac myosin-induced experimental autoimmune myocarditis in rats",
volume = "14",
number = "1",
pages = "9-21",
url = "https://hdl.handle.net/21.15107/rcub_farfar_186"
}

Dimitrijević, M., Milenković, M., Milosavljević, P., Stojić-Vukanić, Z., Colić, M.,& Bartlett, R.. (1998). Beneficial effects of leflunomide on cardiac myosin-induced experimental autoimmune myocarditis in rats. in International Journal of Immunotherapy
Bioscience Ediprint Inc, Carouge., 14(1), 9-21.
https://hdl.handle.net/21.15107/rcub_farfar_186

Dimitrijević M, Milenković M, Milosavljević P, Stojić-Vukanić Z, Colić M, Bartlett R. Beneficial effects of leflunomide on cardiac myosin-induced experimental autoimmune myocarditis in rats. in International Journal of Immunotherapy. 1998;14(1):9-21.
https://hdl.handle.net/21.15107/rcub_farfar_186 .

Dimitrijević, Miroslava, Milenković, Marina, Milosavljević, P, Stojić-Vukanić, Zorica, Colić, M, Bartlett, R, "Beneficial effects of leflunomide on cardiac myosin-induced experimental autoimmune myocarditis in rats" in International Journal of Immunotherapy, 14, no. 1 (1998):9-21,
https://hdl.handle.net/21.15107/rcub_farfar_186 .