TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Licanski, A.
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kostić, Mirjana
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1089
AB  - The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation
VL  - 47
IS  - 3
SP  - 575
EP  - 585
DO  - 10.1016/j.jpba.2008.01.046
ER  - 

@article{
author = "Živanović, Ljiljana and Licanski, A. and Zečević, Mira and Jocić, Biljana and Kostić, Mirjana",
year = "2008",
abstract = "The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation",
volume = "47",
number = "3",
pages = "575-585",
doi = "10.1016/j.jpba.2008.01.046"
}

Živanović, L., Licanski, A., Zečević, M., Jocić, B.,& Kostić, M.. (2008). Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 47(3), 575-585.
https://doi.org/10.1016/j.jpba.2008.01.046

Živanović L, Licanski A, Zečević M, Jocić B, Kostić M. Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis. 2008;47(3):575-585.
doi:10.1016/j.jpba.2008.01.046 .

Živanović, Ljiljana, Licanski, A., Zečević, Mira, Jocić, Biljana, Kostić, Mirjana, "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation" in Journal of Pharmaceutical and Biomedical Analysis, 47, no. 3 (2008):575-585,
https://doi.org/10.1016/j.jpba.2008.01.046 . .

TY  - JOUR
AU  - Jocić, B.
AU  - Zečević, Mira
AU  - Živanović, Lj.
AU  - Licanski, A.
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/982
AB  - An isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the analysis of a novel antimigraine drug, rizatriptan benzoate, in a dosage form along with its two impurities, L-749.019 and L-783.540. The method used a C-18 XTerra (TM) ( 150 x 3.9 mm), 5 mm column. The mobile phase consisted of a mixture of methanol, TEA (1%) and 10 mM KH2PO4 ( 5: 9.5: 85.5 v/v) at a flow rate of 1.2 ml min(-1) ( pH of the water phase was adjusted to 5.5 with 85% orthophosphoric acid). Column temperature was 20 degrees C and the detection was performed at 225 nm. The central composite design technique and the response surface method were used in the robustness test considerations. The method was applied satisfactorily to the analysis of commercial rizatriptan formulation.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Analytical Letters
T1  - A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets
VL  - 40
IS  - 12
SP  - 2301
EP  - 2316
DO  - 10.1080/00032710701575959
ER  - 

@article{
author = "Jocić, B. and Zečević, Mira and Živanović, Lj. and Licanski, A.",
year = "2007",
abstract = "An isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the analysis of a novel antimigraine drug, rizatriptan benzoate, in a dosage form along with its two impurities, L-749.019 and L-783.540. The method used a C-18 XTerra (TM) ( 150 x 3.9 mm), 5 mm column. The mobile phase consisted of a mixture of methanol, TEA (1%) and 10 mM KH2PO4 ( 5: 9.5: 85.5 v/v) at a flow rate of 1.2 ml min(-1) ( pH of the water phase was adjusted to 5.5 with 85% orthophosphoric acid). Column temperature was 20 degrees C and the detection was performed at 225 nm. The central composite design technique and the response surface method were used in the robustness test considerations. The method was applied satisfactorily to the analysis of commercial rizatriptan formulation.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Analytical Letters",
title = "A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets",
volume = "40",
number = "12",
pages = "2301-2316",
doi = "10.1080/00032710701575959"
}

Jocić, B., Zečević, M., Živanović, Lj.,& Licanski, A.. (2007). A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets. in Analytical Letters
Taylor & Francis Inc, Philadelphia., 40(12), 2301-2316.
https://doi.org/10.1080/00032710701575959

Jocić B, Zečević M, Živanović L, Licanski A. A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets. in Analytical Letters. 2007;40(12):2301-2316.
doi:10.1080/00032710701575959 .

Jocić, B., Zečević, Mira, Živanović, Lj., Licanski, A., "A chemometrical approach to optimization and validation of an HPLC assay for rizatriptan and its impurities in tablets" in Analytical Letters, 40, no. 12 (2007):2301-2316,
https://doi.org/10.1080/00032710701575959 . .