TY  - JOUR
AU  - Nikolić, Katarina
AU  - Ivković, Branka
AU  - Besović, Željka
AU  - Marković, Slavko
AU  - Agbaba, Danica
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1202
AB  - A new and accurate HPLC method using beta-cyclodextrin chemically bonded to spherical silica particles as chiral stationary phase (CSP) was developed and validated for determination of S-clopidogrel and its impurities R-enantiomer and S-acid as a hydrolytic product. The effects of acetonitrile and methanol content in the mobile phase and temperature on the resolution and retention of enantiomers were investigated. A satisfactory resolution of S-clopidogrel active form and its impurities was achieved on ChiraDex(R) column (5 mu m, 4 x 250 mm) at a flow rate of 1.0 ml/min and 17 degrees C using acetonitrile, methanol and 0.01 M potassium dihydrogen phosphate solution (15:5:80 v/v/v) as mobile phase. The detection wavelength was set at 220 nm. The method was validated in terms of accuracy, precision, linearity, and robustness. The limit of detection for R-enantiomer and S-acid were 0.75 and 0.09 mu g/ml, respectively, injection volume being 20 mu l. Finally, the molecular modeling of the inclusion complexes between the analytes and beta-cyclodextrin was performed to investigate the mechanism of the enantiorecognition and to study the quantitative structure-retention relationships. Chirality 21:878-885, 2009.
PB  - Wiley, Hoboken
T2  - Chirality
T1  - A Validated Enantiospecific Method for Determination and Purity Assay of Clopridogrel
VL  - 21
IS  - 10
SP  - 878
EP  - 885
DO  - 10.1002/chir.20681
ER  - 

@article{
author = "Nikolić, Katarina and Ivković, Branka and Besović, Željka and Marković, Slavko and Agbaba, Danica",
year = "2009",
abstract = "A new and accurate HPLC method using beta-cyclodextrin chemically bonded to spherical silica particles as chiral stationary phase (CSP) was developed and validated for determination of S-clopidogrel and its impurities R-enantiomer and S-acid as a hydrolytic product. The effects of acetonitrile and methanol content in the mobile phase and temperature on the resolution and retention of enantiomers were investigated. A satisfactory resolution of S-clopidogrel active form and its impurities was achieved on ChiraDex(R) column (5 mu m, 4 x 250 mm) at a flow rate of 1.0 ml/min and 17 degrees C using acetonitrile, methanol and 0.01 M potassium dihydrogen phosphate solution (15:5:80 v/v/v) as mobile phase. The detection wavelength was set at 220 nm. The method was validated in terms of accuracy, precision, linearity, and robustness. The limit of detection for R-enantiomer and S-acid were 0.75 and 0.09 mu g/ml, respectively, injection volume being 20 mu l. Finally, the molecular modeling of the inclusion complexes between the analytes and beta-cyclodextrin was performed to investigate the mechanism of the enantiorecognition and to study the quantitative structure-retention relationships. Chirality 21:878-885, 2009.",
publisher = "Wiley, Hoboken",
journal = "Chirality",
title = "A Validated Enantiospecific Method for Determination and Purity Assay of Clopridogrel",
volume = "21",
number = "10",
pages = "878-885",
doi = "10.1002/chir.20681"
}

Nikolić, K., Ivković, B., Besović, Ž., Marković, S.,& Agbaba, D.. (2009). A Validated Enantiospecific Method for Determination and Purity Assay of Clopridogrel. in Chirality
Wiley, Hoboken., 21(10), 878-885.
https://doi.org/10.1002/chir.20681

Nikolić K, Ivković B, Besović Ž, Marković S, Agbaba D. A Validated Enantiospecific Method for Determination and Purity Assay of Clopridogrel. in Chirality. 2009;21(10):878-885.
doi:10.1002/chir.20681 .

Nikolić, Katarina, Ivković, Branka, Besović, Željka, Marković, Slavko, Agbaba, Danica, "A Validated Enantiospecific Method for Determination and Purity Assay of Clopridogrel" in Chirality, 21, no. 10 (2009):878-885,
https://doi.org/10.1002/chir.20681 . .