TY  - JOUR
AU  - Rmandić, Milena
AU  - Stajić, Ana
AU  - Jančić, Jasna
AU  - Samardžić, Janko
AU  - Jović, Nebojša
AU  - Malenović, Anđelija
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4262
AB  - In this research, a UHPLC–MS/MS method was developed and validated for the deter-
mination of zonisamide in dried plasma spots (DPS) and dried blood spots (DBS). Detection of
zonisamide and internal standard, 1-(2,3-dichlorphenyl)piperazine, was carried out in ESI+ mode by
monitoring two MRM transitions per analyte. Total run time, less than 2.5 min, was achieved using
Acquity UPLC BEH Amide (2.1 × 100 mm, 1.7 μm particle size) column with mobile phase com-
prising acetonitrile–water (85:15%, v/v) with 0.075% formic acid. The flow rate was 0.225 mL/min,
the column temperature was 30 ◦C and the injection volume was 3 μL. Desolvation temperature,
desolvation gas flow rate, ion source temperature and cone gas flow rate were set by the IntelliStart
software tool in combination with tuning. All of the Guthrie cards were scanned, and DPS/DBS
areas were determined by the image processing tool. The influence of hematocrit values (20–60%)
on accuracy and precision was evaluated to determine the range within which method for DBSs is
free from Hct or dependency is within acceptable limits. The validated method was applied to the
determination of zonisamide levels in DPS and DBS samples obtained from patients confirming its
suitability for clinical application. Finally, the distribution of zonisamide into the red blood cells was
estimated by correlating its DPS and DBS levels.
PB  - MDPI
T2  - Molecules
T1  - Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice
VL  - 27
IS  - 15
DO  - 10.3390/molecules27154899
ER  - 

@article{
author = "Rmandić, Milena and Stajić, Ana and Jančić, Jasna and Samardžić, Janko and Jović, Nebojša and Malenović, Anđelija",
year = "2022",
abstract = "In this research, a UHPLC–MS/MS method was developed and validated for the deter-
mination of zonisamide in dried plasma spots (DPS) and dried blood spots (DBS). Detection of
zonisamide and internal standard, 1-(2,3-dichlorphenyl)piperazine, was carried out in ESI+ mode by
monitoring two MRM transitions per analyte. Total run time, less than 2.5 min, was achieved using
Acquity UPLC BEH Amide (2.1 × 100 mm, 1.7 μm particle size) column with mobile phase com-
prising acetonitrile–water (85:15%, v/v) with 0.075% formic acid. The flow rate was 0.225 mL/min,
the column temperature was 30 ◦C and the injection volume was 3 μL. Desolvation temperature,
desolvation gas flow rate, ion source temperature and cone gas flow rate were set by the IntelliStart
software tool in combination with tuning. All of the Guthrie cards were scanned, and DPS/DBS
areas were determined by the image processing tool. The influence of hematocrit values (20–60%)
on accuracy and precision was evaluated to determine the range within which method for DBSs is
free from Hct or dependency is within acceptable limits. The validated method was applied to the
determination of zonisamide levels in DPS and DBS samples obtained from patients confirming its
suitability for clinical application. Finally, the distribution of zonisamide into the red blood cells was
estimated by correlating its DPS and DBS levels.",
publisher = "MDPI",
journal = "Molecules",
title = "Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice",
volume = "27",
number = "15",
doi = "10.3390/molecules27154899"
}

Rmandić, M., Stajić, A., Jančić, J., Samardžić, J., Jović, N.,& Malenović, A.. (2022). Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice. in Molecules
MDPI., 27(15).
https://doi.org/10.3390/molecules27154899

Rmandić M, Stajić A, Jančić J, Samardžić J, Jović N, Malenović A. Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice. in Molecules. 2022;27(15).
doi:10.3390/molecules27154899 .

Rmandić, Milena, Stajić, Ana, Jančić, Jasna, Samardžić, Janko, Jović, Nebojša, Malenović, Anđelija, "Quantification of Zonisamide in Dried Blood Spots and Dried Plasma Spots by UPLC–MS/MS: Application to Clinical Practice" in Molecules, 27, no. 15 (2022),
https://doi.org/10.3390/molecules27154899 . .

TY  - JOUR
AU  - Stevanović, Dejan
AU  - Jančić, Jasna
AU  - Topalović, Mirko
AU  - Tadić, Ivana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1630
AB  - The levels of agreement between self- and parent/proxy-reports of anxiety and depressive symptoms in pediatric epilepsy were evaluated. Data were drawn from 56 pairs of children with epilepsy and at least one parent. Anxiety symptoms were assessed using the Screen for Child Anxiety Related Emotional Disorders (SCARED), while depressive symptoms were assessed using the Mood and Feeling Questionnaire (MFQ). Moderate to substantial levels of agreement between raters when reporting various anxiety symptoms, such as panic/somatic disorder, generalized anxiety disorder, separation anxiety disorder, social phobia, and school phobia symptoms, were observed. Levels of agreement between raters were substantial when reporting depressive symptoms. However, levels of agreement differed if raw or criterion-referenced questionnaires' scores were used. In case of using raw questionnaire scores, substantial agreements appeared when reporting overall anxiety and depressive symptoms. On the other hand, moderate agreements appeared when reporting particular anxiety symptoms with raw questionnaire scores or when using criterion-referenced scores that indicate the presence of certain symptoms in a clinical range. Therefore, it is advisable to include both raters when assessing anxiety and depressive symptoms in pediatric epilepsy.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Epilepsy & Behavior
T1  - Agreement between children and parents when reporting anxiety and depressive symptoms in pediatric epilepsy
VL  - 25
IS  - 2
SP  - 141
EP  - 144
DO  - 10.1016/j.yebeh.2012.07.017
ER  - 

@article{
author = "Stevanović, Dejan and Jančić, Jasna and Topalović, Mirko and Tadić, Ivana",
year = "2012",
abstract = "The levels of agreement between self- and parent/proxy-reports of anxiety and depressive symptoms in pediatric epilepsy were evaluated. Data were drawn from 56 pairs of children with epilepsy and at least one parent. Anxiety symptoms were assessed using the Screen for Child Anxiety Related Emotional Disorders (SCARED), while depressive symptoms were assessed using the Mood and Feeling Questionnaire (MFQ). Moderate to substantial levels of agreement between raters when reporting various anxiety symptoms, such as panic/somatic disorder, generalized anxiety disorder, separation anxiety disorder, social phobia, and school phobia symptoms, were observed. Levels of agreement between raters were substantial when reporting depressive symptoms. However, levels of agreement differed if raw or criterion-referenced questionnaires' scores were used. In case of using raw questionnaire scores, substantial agreements appeared when reporting overall anxiety and depressive symptoms. On the other hand, moderate agreements appeared when reporting particular anxiety symptoms with raw questionnaire scores or when using criterion-referenced scores that indicate the presence of certain symptoms in a clinical range. Therefore, it is advisable to include both raters when assessing anxiety and depressive symptoms in pediatric epilepsy.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Epilepsy & Behavior",
title = "Agreement between children and parents when reporting anxiety and depressive symptoms in pediatric epilepsy",
volume = "25",
number = "2",
pages = "141-144",
doi = "10.1016/j.yebeh.2012.07.017"
}

Stevanović, D., Jančić, J., Topalović, M.,& Tadić, I.. (2012). Agreement between children and parents when reporting anxiety and depressive symptoms in pediatric epilepsy. in Epilepsy & Behavior
Academic Press Inc Elsevier Science, San Diego., 25(2), 141-144.
https://doi.org/10.1016/j.yebeh.2012.07.017

Stevanović D, Jančić J, Topalović M, Tadić I. Agreement between children and parents when reporting anxiety and depressive symptoms in pediatric epilepsy. in Epilepsy & Behavior. 2012;25(2):141-144.
doi:10.1016/j.yebeh.2012.07.017 .

Stevanović, Dejan, Jančić, Jasna, Topalović, Mirko, Tadić, Ivana, "Agreement between children and parents when reporting anxiety and depressive symptoms in pediatric epilepsy" in Epilepsy & Behavior, 25, no. 2 (2012):141-144,
https://doi.org/10.1016/j.yebeh.2012.07.017 . .