TY  - JOUR
AU  - Aranđelović, Jovana
AU  - Santrač, Anja
AU  - Batinić, Bojan
AU  - Todorović, Lidija
AU  - Stevanović, Vladimir
AU  - Tiruveedhula, Veera Venkata Naga Phani Babu
AU  - Sharmin, Dishary
AU  - Rashid, Farjana
AU  - Stanojević, Boban
AU  - Cook, James
AU  - Savić, Miroslav
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4203
AB  - Aims: GABAergic modulation involved in cognitive processing appears to be substan- tially changed in Alzheimer's disease (AD). In a widely used 5xFAD model of AD, we aimed to assess if negative and positive allosteric modulators of α5 GABA A receptors (NAM and PAM, respectively) would affect social interaction, social, object and spatial memory, and neuroinflammation. Methods: After 10-day treatment with PAM, NAM, or solvent, 6-month-old trans- genic and non-transgenic 5xFAD mice underwent testing in a behavioral battery. Gene expressions of IL-1β, IL-6, TNF-α, GFAP, and IBA-1 were determined in hippocampus and prefrontal cortex by qPCR. Results: PAM treatment impaired spatial learning in transgenic females compared to solvent-treated transgenic females, and social recognition in transgenic and non- transgenic males. NAM treatment declined social interaction in transgenic and non- transgenic males, while had beneficial effect on cognitive flexibility in non-transgenic males compared to solvent-treated non-transgenic males. Transgenic animals have not fully displayed cognitive symptoms, but neuroinflammation was confirmed. NAM reduced proinflammatory gene expressions in transgenic females and astrogliosis in transgenic males compared to pathological controls. Conclusion: PAM and NAM failed to exert favorable behavioral effects in transgenic animals. Suppression of neuroinflammation obtained with NAM calls for more studies with GABAergic ligands in amyloid beta- and/or tau-dependent models with promi- nent neuroinflammation.
PB  - John Wiley and Sons Inc
T2  - CNS Neuroscience & Therapeutics
T1  - Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease
VL  - 28
IS  - 11
SP  - 1767
EP  - 1778
DO  - 10.1111/cns.13914
ER  - 

@article{
author = "Aranđelović, Jovana and Santrač, Anja and Batinić, Bojan and Todorović, Lidija and Stevanović, Vladimir and Tiruveedhula, Veera Venkata Naga Phani Babu and Sharmin, Dishary and Rashid, Farjana and Stanojević, Boban and Cook, James and Savić, Miroslav",
year = "2022",
abstract = "Aims: GABAergic modulation involved in cognitive processing appears to be substan- tially changed in Alzheimer's disease (AD). In a widely used 5xFAD model of AD, we aimed to assess if negative and positive allosteric modulators of α5 GABA A receptors (NAM and PAM, respectively) would affect social interaction, social, object and spatial memory, and neuroinflammation. Methods: After 10-day treatment with PAM, NAM, or solvent, 6-month-old trans- genic and non-transgenic 5xFAD mice underwent testing in a behavioral battery. Gene expressions of IL-1β, IL-6, TNF-α, GFAP, and IBA-1 were determined in hippocampus and prefrontal cortex by qPCR. Results: PAM treatment impaired spatial learning in transgenic females compared to solvent-treated transgenic females, and social recognition in transgenic and non- transgenic males. NAM treatment declined social interaction in transgenic and non- transgenic males, while had beneficial effect on cognitive flexibility in non-transgenic males compared to solvent-treated non-transgenic males. Transgenic animals have not fully displayed cognitive symptoms, but neuroinflammation was confirmed. NAM reduced proinflammatory gene expressions in transgenic females and astrogliosis in transgenic males compared to pathological controls. Conclusion: PAM and NAM failed to exert favorable behavioral effects in transgenic animals. Suppression of neuroinflammation obtained with NAM calls for more studies with GABAergic ligands in amyloid beta- and/or tau-dependent models with promi- nent neuroinflammation.",
publisher = "John Wiley and Sons Inc",
journal = "CNS Neuroscience & Therapeutics",
title = "Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease",
volume = "28",
number = "11",
pages = "1767-1778",
doi = "10.1111/cns.13914"
}

Aranđelović, J., Santrač, A., Batinić, B., Todorović, L., Stevanović, V., Tiruveedhula, V. V. N. P. B., Sharmin, D., Rashid, F., Stanojević, B., Cook, J.,& Savić, M.. (2022). Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease. in CNS Neuroscience & Therapeutics
John Wiley and Sons Inc., 28(11), 1767-1778.
https://doi.org/10.1111/cns.13914

Aranđelović J, Santrač A, Batinić B, Todorović L, Stevanović V, Tiruveedhula VVNPB, Sharmin D, Rashid F, Stanojević B, Cook J, Savić M. Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease. in CNS Neuroscience & Therapeutics. 2022;28(11):1767-1778.
doi:10.1111/cns.13914 .

Aranđelović, Jovana, Santrač, Anja, Batinić, Bojan, Todorović, Lidija, Stevanović, Vladimir, Tiruveedhula, Veera Venkata Naga Phani Babu, Sharmin, Dishary, Rashid, Farjana, Stanojević, Boban, Cook, James, Savić, Miroslav, "Effects of α5 GABAA receptor modulation on social interaction, memory, and neuroinflammation in a mouse model of Alzheimer's disease" in CNS Neuroscience & Therapeutics, 28, no. 11 (2022):1767-1778,
https://doi.org/10.1111/cns.13914 . .

TY  - JOUR
AU  - Santrač, Anja
AU  - Bijelić, Dunja
AU  - Stevanović, Vladimir
AU  - Banićević, Marija
AU  - Aranđelović, Jovana
AU  - Batinić, Bojan
AU  - Sharmin, Dishary
AU  - Cook, James
AU  - Savić, Miroslav
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4071
AB  - Autism spectrum disorder (ASD), as a common neurodevelopmental disorder that encompasses impairments in social communication and interaction, as well as repetitive and restrictive behavior, still awaits an effective treatment strategy. The involvement of GABAergic neurotransmission, and especially a deficit of GABA A receptors that contain the α5 subunits, were implicated in pathogenesis of ASD. Therefore, we tested MP-III-022, a positive allosteric modulator (PAM) selective for α5GABAA receptors, in Wistar rats prenatally exposed to valproic acid, as an animal model useful for studying ASD. Postweaning rats of both sexes were treated for 7 days with vehicle or MP-III-022 at two doses pharmacokinetically determined as selective, and thereafter tested in a behavioral battery (social interaction test, elevated plus maze, spontaneous locomotor activ- ity, and standard and reverse Morris water maze). Additional rats were used for establishing a primary neuronal culture and performing calcium imaging, and determination of hippocampal mRNA levels of GABRA5, NKCC1, and KCC2. MP-III-022 prevented impairments in many parameters connected with social, repetitive and restrictive behavioral domains. The lower and higher dose was more effective in males and females, respectively. Intriguingly, MP-III-022 elicited certain changes in control animals similar to those manifested in valproate ani- mals themselves. Behavioral results were mirrored in GABA switch and spontane- ous neuronal activity, assessed with calcium imaging, and also in expression changes of three genes analyzed. Our data support a role of α5GABAA receptors in pathophysiology of ASD, and suggest a potential application of selective PAMs in its treatment, that needs to be researched in a sex-specific manner.
PB  - John Wiley and Sons Inc
T2  - Autism Research
T1  - Postweaning positive modulation of α5GABAA receptors improves autism-like features in prenatal valproate rat model in a sex-specific manner
DO  - 10.1002/aur.2699
ER  - 

@article{
author = "Santrač, Anja and Bijelić, Dunja and Stevanović, Vladimir and Banićević, Marija and Aranđelović, Jovana and Batinić, Bojan and Sharmin, Dishary and Cook, James and Savić, Miroslav",
year = "2022",
abstract = "Autism spectrum disorder (ASD), as a common neurodevelopmental disorder that encompasses impairments in social communication and interaction, as well as repetitive and restrictive behavior, still awaits an effective treatment strategy. The involvement of GABAergic neurotransmission, and especially a deficit of GABA A receptors that contain the α5 subunits, were implicated in pathogenesis of ASD. Therefore, we tested MP-III-022, a positive allosteric modulator (PAM) selective for α5GABAA receptors, in Wistar rats prenatally exposed to valproic acid, as an animal model useful for studying ASD. Postweaning rats of both sexes were treated for 7 days with vehicle or MP-III-022 at two doses pharmacokinetically determined as selective, and thereafter tested in a behavioral battery (social interaction test, elevated plus maze, spontaneous locomotor activ- ity, and standard and reverse Morris water maze). Additional rats were used for establishing a primary neuronal culture and performing calcium imaging, and determination of hippocampal mRNA levels of GABRA5, NKCC1, and KCC2. MP-III-022 prevented impairments in many parameters connected with social, repetitive and restrictive behavioral domains. The lower and higher dose was more effective in males and females, respectively. Intriguingly, MP-III-022 elicited certain changes in control animals similar to those manifested in valproate ani- mals themselves. Behavioral results were mirrored in GABA switch and spontane- ous neuronal activity, assessed with calcium imaging, and also in expression changes of three genes analyzed. Our data support a role of α5GABAA receptors in pathophysiology of ASD, and suggest a potential application of selective PAMs in its treatment, that needs to be researched in a sex-specific manner.",
publisher = "John Wiley and Sons Inc",
journal = "Autism Research",
title = "Postweaning positive modulation of α5GABAA receptors improves autism-like features in prenatal valproate rat model in a sex-specific manner",
doi = "10.1002/aur.2699"
}

Santrač, A., Bijelić, D., Stevanović, V., Banićević, M., Aranđelović, J., Batinić, B., Sharmin, D., Cook, J.,& Savić, M.. (2022). Postweaning positive modulation of α5GABAA receptors improves autism-like features in prenatal valproate rat model in a sex-specific manner. in Autism Research
John Wiley and Sons Inc..
https://doi.org/10.1002/aur.2699

Santrač A, Bijelić D, Stevanović V, Banićević M, Aranđelović J, Batinić B, Sharmin D, Cook J, Savić M. Postweaning positive modulation of α5GABAA receptors improves autism-like features in prenatal valproate rat model in a sex-specific manner. in Autism Research. 2022;.
doi:10.1002/aur.2699 .

Santrač, Anja, Bijelić, Dunja, Stevanović, Vladimir, Banićević, Marija, Aranđelović, Jovana, Batinić, Bojan, Sharmin, Dishary, Cook, James, Savić, Miroslav, "Postweaning positive modulation of α5GABAA receptors improves autism-like features in prenatal valproate rat model in a sex-specific manner" in Autism Research (2022),
https://doi.org/10.1002/aur.2699 . .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Slavkovska, Violeta
AU  - Stevanović, Vladimir
AU  - Stevanović, Branka
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1007
AB  - Teucrium arduini L. is an endemic of the Mediteranean to sub-Mediterranean part of the Western Balkans. The ecological plasticity, i.e., inter-population differentiation, of the species Teucrium arduini was analyzed on the basis of morpho-anatomical variability of six populations from different types of vegetation, i.e., of rocky crevices, rocky ground, and a thermophilous forest in the eu-Mediterranean, sub-Mediterranean, and transitional sub-Mediterranean-Central-European climate zones. Univariant statistic analysis included 22 quantitative characters related to leaf and stem anatomy and morphology. In order to establish the variability and significance of morpho-anatomical differentiation, principal component analyses (PCA), multivariant analyses of variances (MANOVA), and discriminant components analysis (DCA) were done. The analyses of plants from these spatially separated populations confirmed that the species T. arduini belongs to the category of xerophytes with malacophyllous leaves.
AB  - Teucrium arduini L. je endemična vrsta rasprostranjena u mediteranskim i submediteranskim područjima zapadnog dela Balkanskog poluostrva, od Istre na severu do Albanije na jugu. Vrsta pripada sekciji Stachyobotrus Bentham kojoj na prostoru Evrope pripada još samo maloazijsko-istočno balkanska vrsta T. laminifolium D’Urv., u koju je uključeni takson T. cordifolium Čelak. Ekološka plastičnost i interpopulaciona diferencijacija vrste T. arduini je analizirana na osnovu morfo-anatomske varijabilnosti šest populacija (Risan, Njeguši i kanjoni Cijevne, Morače i Komarnice u Crnoj Gori i Rugovska klisura u Srbiji) koje se razvijaju u različitim tipovima vegetacije, i to u pukotinama karbonatnih stena (Asplenietea rupestris), kamenjara (Festuco-Brometea) i termofilnim šumama na krečnjacima (Ostryo-Carpinion orientalis) u uslovima eumediteranske, submediteranske i prelazne mediteransko-srednjeevropske klime. Statističkom analizom su obuhvaćena 22 kvantitativna karaktera vezana za anatomiju i morfologiju listova i stabla. U cilju utvrđivanja prirode varijabilnosti i stepena statističke značajnosti ustanovljenih razlika urađena je multivarijaciona analiza koja je obuhvatila analizu varijansi (MANOVA), analizu osnovnih komponenti (PCA) i diskriminantnu analizu osnovnih komponenti (DCA). Na osnovu urađenih analiza biljaka iz šest populacija koje se razvijaju na različitim staništima i u različitim ekološkima uslovima, utvrđeno je da vrsta T. arduini pripada grupi kserofita sa malakofilnom građom listova. Na osnovu rezultata diskriminantne analize ustanovljeno je da su analizirane populacije morfo-anatomski veoma neznatno izdiferencirane u tri grupe biljaka između kojih postoje veoma male razlike. Na taj način se pokazalo da i vrsta T. arduini ima veoma stabilne, konzervativne morfo-anatomske, kseromorfne strukture, koje su karakteristične i za druge endemične biljke Balkanskog poluostrva.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae)
T1  - Morfo-anatomska diferencijacija balkanskog endemita Teucrium arduini L. (Lamiaceae)
VL  - 59
IS  - 4
SP  - 369
EP  - 381
DO  - 10.2298/ABS0704369L
ER  - 

@article{
author = "Lakušić, Branislava and Lakušić, Dmitar and Slavkovska, Violeta and Stevanović, Vladimir and Stevanović, Branka",
year = "2007",
abstract = "Teucrium arduini L. is an endemic of the Mediteranean to sub-Mediterranean part of the Western Balkans. The ecological plasticity, i.e., inter-population differentiation, of the species Teucrium arduini was analyzed on the basis of morpho-anatomical variability of six populations from different types of vegetation, i.e., of rocky crevices, rocky ground, and a thermophilous forest in the eu-Mediterranean, sub-Mediterranean, and transitional sub-Mediterranean-Central-European climate zones. Univariant statistic analysis included 22 quantitative characters related to leaf and stem anatomy and morphology. In order to establish the variability and significance of morpho-anatomical differentiation, principal component analyses (PCA), multivariant analyses of variances (MANOVA), and discriminant components analysis (DCA) were done. The analyses of plants from these spatially separated populations confirmed that the species T. arduini belongs to the category of xerophytes with malacophyllous leaves., Teucrium arduini L. je endemična vrsta rasprostranjena u mediteranskim i submediteranskim područjima zapadnog dela Balkanskog poluostrva, od Istre na severu do Albanije na jugu. Vrsta pripada sekciji Stachyobotrus Bentham kojoj na prostoru Evrope pripada još samo maloazijsko-istočno balkanska vrsta T. laminifolium D’Urv., u koju je uključeni takson T. cordifolium Čelak. Ekološka plastičnost i interpopulaciona diferencijacija vrste T. arduini je analizirana na osnovu morfo-anatomske varijabilnosti šest populacija (Risan, Njeguši i kanjoni Cijevne, Morače i Komarnice u Crnoj Gori i Rugovska klisura u Srbiji) koje se razvijaju u različitim tipovima vegetacije, i to u pukotinama karbonatnih stena (Asplenietea rupestris), kamenjara (Festuco-Brometea) i termofilnim šumama na krečnjacima (Ostryo-Carpinion orientalis) u uslovima eumediteranske, submediteranske i prelazne mediteransko-srednjeevropske klime. Statističkom analizom su obuhvaćena 22 kvantitativna karaktera vezana za anatomiju i morfologiju listova i stabla. U cilju utvrđivanja prirode varijabilnosti i stepena statističke značajnosti ustanovljenih razlika urađena je multivarijaciona analiza koja je obuhvatila analizu varijansi (MANOVA), analizu osnovnih komponenti (PCA) i diskriminantnu analizu osnovnih komponenti (DCA). Na osnovu urađenih analiza biljaka iz šest populacija koje se razvijaju na različitim staništima i u različitim ekološkima uslovima, utvrđeno je da vrsta T. arduini pripada grupi kserofita sa malakofilnom građom listova. Na osnovu rezultata diskriminantne analize ustanovljeno je da su analizirane populacije morfo-anatomski veoma neznatno izdiferencirane u tri grupe biljaka između kojih postoje veoma male razlike. Na taj način se pokazalo da i vrsta T. arduini ima veoma stabilne, konzervativne morfo-anatomske, kseromorfne strukture, koje su karakteristične i za druge endemične biljke Balkanskog poluostrva.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae), Morfo-anatomska diferencijacija balkanskog endemita Teucrium arduini L. (Lamiaceae)",
volume = "59",
number = "4",
pages = "369-381",
doi = "10.2298/ABS0704369L"
}

Lakušić, B., Lakušić, D., Slavkovska, V., Stevanović, V.,& Stevanović, B.. (2007). Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(4), 369-381.
https://doi.org/10.2298/ABS0704369L

Lakušić B, Lakušić D, Slavkovska V, Stevanović V, Stevanović B. Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae). in Archives of Biological Sciences. 2007;59(4):369-381.
doi:10.2298/ABS0704369L .

Lakušić, Branislava, Lakušić, Dmitar, Slavkovska, Violeta, Stevanović, Vladimir, Stevanović, Branka, "Morpho-anatomical differentiation of the Balkan endemic species Teucrium arduini L. (Lamiaceae)" in Archives of Biological Sciences, 59, no. 4 (2007):369-381,
https://doi.org/10.2298/ABS0704369L . .