TY  - JOUR
AU  - Živković, Irena
AU  - Petrović, Raisa
AU  - Arsenović-Ranin, Nevena
AU  - Petrusić, Vladimir
AU  - Minić, Rajna
AU  - Bufan, Biljana
AU  - Popović, Olga
AU  - Leposavić, Gordana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3160
AB  - The study explored influence of biological sex on development of humoral immune response to seasonal trivalent whole inactivated virus (WIV) and split virus (SV) influenza vaccines in outbred Swiss mouse model. To this end, mice of both sexes were immunized with WIV (WIV mice) and SV vaccines (SV mice) and examined for specific antibody response. Irrespective of sex, total IgG and neutralizing antibody responses to distinct virus strains were weaker in SV than in WIV mice. In WIV mice of both sexes, irrespective of strain specificity, IgG isotype response was dominated by IgG2a antibodies, while in SV mice nearly equal representation of IgG2a and IgG1 antibodies was found. The analyses of sex differences showed higher titers of H1N1-specific and both H1N1- and H3N2-specific total IgG and neutralizing antibodies in female WIV and SV mice, respectively. Additionally, sexual dimorphism in IgG subclass profile depended on vaccine type. Specifically, compared with males, in females WIV shifted IgG2a/IgG1 antibody ratio towards IgG2a isotype on the account of weaker IgG1 response, whereas in SV mice, irrespective of virus strain, IgG2a and IgG1 isotypes were equally represented in both sexes. These findings indicate the vaccine type-dependent sex bias in antibody response to inactivated influenza vaccines.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Biologicals
T1  - Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type
VL  - 52
SP  - 18
EP  - 24
DO  - 10.1016/j.biologicals.2018.01.007
ER  - 

@article{
author = "Živković, Irena and Petrović, Raisa and Arsenović-Ranin, Nevena and Petrusić, Vladimir and Minić, Rajna and Bufan, Biljana and Popović, Olga and Leposavić, Gordana",
year = "2018",
abstract = "The study explored influence of biological sex on development of humoral immune response to seasonal trivalent whole inactivated virus (WIV) and split virus (SV) influenza vaccines in outbred Swiss mouse model. To this end, mice of both sexes were immunized with WIV (WIV mice) and SV vaccines (SV mice) and examined for specific antibody response. Irrespective of sex, total IgG and neutralizing antibody responses to distinct virus strains were weaker in SV than in WIV mice. In WIV mice of both sexes, irrespective of strain specificity, IgG isotype response was dominated by IgG2a antibodies, while in SV mice nearly equal representation of IgG2a and IgG1 antibodies was found. The analyses of sex differences showed higher titers of H1N1-specific and both H1N1- and H3N2-specific total IgG and neutralizing antibodies in female WIV and SV mice, respectively. Additionally, sexual dimorphism in IgG subclass profile depended on vaccine type. Specifically, compared with males, in females WIV shifted IgG2a/IgG1 antibody ratio towards IgG2a isotype on the account of weaker IgG1 response, whereas in SV mice, irrespective of virus strain, IgG2a and IgG1 isotypes were equally represented in both sexes. These findings indicate the vaccine type-dependent sex bias in antibody response to inactivated influenza vaccines.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Biologicals",
title = "Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type",
volume = "52",
pages = "18-24",
doi = "10.1016/j.biologicals.2018.01.007"
}

Živković, I., Petrović, R., Arsenović-Ranin, N., Petrusić, V., Minić, R., Bufan, B., Popović, O.,& Leposavić, G.. (2018). Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type. in Biologicals
Academic Press Ltd- Elsevier Science Ltd, London., 52, 18-24.
https://doi.org/10.1016/j.biologicals.2018.01.007

Živković I, Petrović R, Arsenović-Ranin N, Petrusić V, Minić R, Bufan B, Popović O, Leposavić G. Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type. in Biologicals. 2018;52:18-24.
doi:10.1016/j.biologicals.2018.01.007 .

Živković, Irena, Petrović, Raisa, Arsenović-Ranin, Nevena, Petrusić, Vladimir, Minić, Rajna, Bufan, Biljana, Popović, Olga, Leposavić, Gordana, "Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type" in Biologicals, 52 (2018):18-24,
https://doi.org/10.1016/j.biologicals.2018.01.007 . .

TY  - JOUR
AU  - Živković, Irena
AU  - Bufan, Biljana
AU  - Petrusić, Vladimir
AU  - Minić, Rajna
AU  - Arsenović-Ranin, Nevena
AU  - Petrović, Raisa
AU  - Leposavić, Gordana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2301
AB  - An outbred mouse model was used to determine if antibody response to immunization with whole-virus trivalent inactivated influenza vaccine (TIV) differs between the sexes. The antibody response was examined one (serum titer of IgM antibodies), and three and six weeks post-immunization (serum titer of neutralizing and total IgG antibodies and IgG subclass profile). Compared with male in female mice was found (i) the more robust IgM response against all influenza strains included in TIV and (ii) more vigorous neutralizing antibody and total IgG responses against H1N1 influenza virus at both the examined time points post-immunization. The total IgG antibody response against H3N2 and B influenza viruses was comparable between female and male mice three weeks post-immunization, but significantly greater in female mice six weeks post-immunization. The neutralizing antibody response against H3N2 and B influenza viruses did not significantly differ between sexes at both the examined points post-immunization. Finally, three weeks post-immunization subclass profile of IgG specific to the influenza strains included in TIV differed between female and male mice, reflecting the lower titer of IgG1 antibodies in female ones, so that IgG2a (contributing mainly to the total IgG) to IgG1 ratio in mice of this sex was shifted toward the former. In agreement with this shift, compared with male mice, Th1/Th2 balance in female mice was shifted toward Th1, as shown by ELISPOT. Collectively, the results showed influenza virus strain-dependent sexual dimorphism in the magnitude, dynamics and characteristics of antibody response in outbred mice immunized with TIV.
PB  - Elsevier Sci Ltd, Oxford
T2  - Vaccine
T1  - Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice
VL  - 33
IS  - 42
SP  - 5546
EP  - 5552
DO  - 10.1016/j.vaccine.2015.09.006
ER  - 

@article{
author = "Živković, Irena and Bufan, Biljana and Petrusić, Vladimir and Minić, Rajna and Arsenović-Ranin, Nevena and Petrović, Raisa and Leposavić, Gordana",
year = "2015",
abstract = "An outbred mouse model was used to determine if antibody response to immunization with whole-virus trivalent inactivated influenza vaccine (TIV) differs between the sexes. The antibody response was examined one (serum titer of IgM antibodies), and three and six weeks post-immunization (serum titer of neutralizing and total IgG antibodies and IgG subclass profile). Compared with male in female mice was found (i) the more robust IgM response against all influenza strains included in TIV and (ii) more vigorous neutralizing antibody and total IgG responses against H1N1 influenza virus at both the examined time points post-immunization. The total IgG antibody response against H3N2 and B influenza viruses was comparable between female and male mice three weeks post-immunization, but significantly greater in female mice six weeks post-immunization. The neutralizing antibody response against H3N2 and B influenza viruses did not significantly differ between sexes at both the examined points post-immunization. Finally, three weeks post-immunization subclass profile of IgG specific to the influenza strains included in TIV differed between female and male mice, reflecting the lower titer of IgG1 antibodies in female ones, so that IgG2a (contributing mainly to the total IgG) to IgG1 ratio in mice of this sex was shifted toward the former. In agreement with this shift, compared with male mice, Th1/Th2 balance in female mice was shifted toward Th1, as shown by ELISPOT. Collectively, the results showed influenza virus strain-dependent sexual dimorphism in the magnitude, dynamics and characteristics of antibody response in outbred mice immunized with TIV.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Vaccine",
title = "Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice",
volume = "33",
number = "42",
pages = "5546-5552",
doi = "10.1016/j.vaccine.2015.09.006"
}

Živković, I., Bufan, B., Petrusić, V., Minić, R., Arsenović-Ranin, N., Petrović, R.,& Leposavić, G.. (2015). Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice. in Vaccine
Elsevier Sci Ltd, Oxford., 33(42), 5546-5552.
https://doi.org/10.1016/j.vaccine.2015.09.006

Živković I, Bufan B, Petrusić V, Minić R, Arsenović-Ranin N, Petrović R, Leposavić G. Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice. in Vaccine. 2015;33(42):5546-5552.
doi:10.1016/j.vaccine.2015.09.006 .

Živković, Irena, Bufan, Biljana, Petrusić, Vladimir, Minić, Rajna, Arsenović-Ranin, Nevena, Petrović, Raisa, Leposavić, Gordana, "Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice" in Vaccine, 33, no. 42 (2015):5546-5552,
https://doi.org/10.1016/j.vaccine.2015.09.006 . .

TY  - JOUR
AU  - Dimitrijević, Rajna
AU  - Ivanović, Nevena
AU  - Mathiesen, Geir
AU  - Petrusić, Vladimir
AU  - Živković, Irena
AU  - Đorđević, Brižita
AU  - Dimitrijević, Ljiljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2165
AB  - Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration
VL  - 81
IS  - 2
SP  - 202
EP  - 207
DO  - 10.1017/S0022029914000028
ER  - 

@article{
author = "Dimitrijević, Rajna and Ivanović, Nevena and Mathiesen, Geir and Petrusić, Vladimir and Živković, Irena and Đorđević, Brižita and Dimitrijević, Ljiljana",
year = "2014",
abstract = "Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration",
volume = "81",
number = "2",
pages = "202-207",
doi = "10.1017/S0022029914000028"
}

Dimitrijević, R., Ivanović, N., Mathiesen, G., Petrusić, V., Živković, I., Đorđević, B.,& Dimitrijević, L.. (2014). Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research
Cambridge Univ Press, New York., 81(2), 202-207.
https://doi.org/10.1017/S0022029914000028

Dimitrijević R, Ivanović N, Mathiesen G, Petrusić V, Živković I, Đorđević B, Dimitrijević L. Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research. 2014;81(2):202-207.
doi:10.1017/S0022029914000028 .

Dimitrijević, Rajna, Ivanović, Nevena, Mathiesen, Geir, Petrusić, Vladimir, Živković, Irena, Đorđević, Brižita, Dimitrijević, Ljiljana, "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration" in Journal of Dairy Research, 81, no. 2 (2014):202-207,
https://doi.org/10.1017/S0022029914000028 . .