@conference{
author = "Georgijev, Dejan and Kopanja, Đurđica and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "In the pharmacies of Republic of Serbia, three dietary products and food supplements with blueberry fruit preparations intended to improve eyesight were found and the content of anthocyanins in them was analyzed. According to Ph. Eur. 10, colorimetric method was used for determination of anthocyanins, which was given in the monograph of Myrthilli fructus recens. For compairing, the content of anthocyanins in two juices with blueberry fruit and other fruits with anthocyanins (aronia, grape) was analysed using an AOAC colorimetric method given by Lee et al. (2005).
Retin activ (ESI srl, Italy) a dietary product with a special medical purpose, contains vitamins A, C, E, 15 mg of dry blueberry extract standardized at 25% anthocyanosides, lutein and zeaxanthin. The advice of the manufacturer for use is one capsule a day. Our analysis have shown that Retin activ in one capsule contain 8.35 ±0,17 mg of anthocyanins, calculated as cyanidin 3-O-glucoside chloride.
Pro-Visio tablets (United Pharma, Bratislava, Slovakia), a food supplement with lutein, zeaxanthin, 20 mg of blueberry extract, vitamin E and selenium, contained 5.14±0.18 mg of anthocyanins, calculated as cyanidin 3-O-glucoside chloride in one tablet. The advice of the manufacturer for use is one capsule a day.
Visionace Original tablets (Vitabiotics, London, UK) contain 23 components including blueberry extract (60 mg) and 0.02±0.0089 mg of anthocyanins per tablets.
The juices, Bravo - blueberry (RAUCH SERBIA DOO) and Nectar - apple, aronia, blueberry and grape (Nectar DOO, Serbia), contained 42.57±2.66 mg/L and 31.80±0.01 mg/L of anthocyanins, respectively.
According to the literature, blueberry anthocyanins has been reported to enhance night visual acuity and contrast sensitivity and are used in the supplements to improve vision health. Older studies (Vannini et al., 1986) have shown significant improvement of the pupillary muscle contraction in reduced light conditions in healthy young subjects after administration of 240 mg of blueberry extract containing 36% anthocyanins. The claimed effect refers to an improvement of visual adaptation to the dark. Newer studies were contradictory.
JECFA has established an Acceptable Daily Intake (ADI) of 2.5 mg/kg bw/day for anthocyanins from grape skin. The dose of 50 mg of anthocyanins of black current concentrate have shown positive effect after 30 min of dark adaptation compared before and 2 h after intake of test drink in 12 healthy subjects (Nomi et al., 2019).
In conclusion, our results suggest that the best option for better eye health is to use products with concentrated blueberry fruit extracts instead of juices, but the intake of at least 50 mg of anthocyanins per day should be ensured.
References
1. Lee J., Durst RW, Wrolstad E., Journal of AOAC International, 88:5, 1269-1278, 2005
2. Vannini L., Samuelly R., Coffano M., Tibaldi L. Bollett Ocul, 65 (6):569-577, 1986
3. Nomi Y, Iwasaki-Kurashige K., Matsumoto H., Molecules, 24(18): 3311, 2019",
publisher = "The International Pharmaceutical Federation (FIP)",
journal = "80th FIP World Congress of Pharmacy and Pharmaceutical Sciences, September 18-22, 2022. Seville, Spain",
title = "The quantity of anthocyanins in blueberry fruit based dietary products and juices",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5507"
}
