TY  - JOUR
AU  - Milić, B
AU  - Nedeljković, M
AU  - Đukić, Mirjana
PY  - 1995
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/124
AB  - A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in determination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise.
T2  - Jugoslovenska medicinska biohemija
T1  - HPLC method for determination of oximes HI-6 and trimedoxime in plasma
T1  - HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi
VL  - 14
IS  - 3-4
SP  - 125
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_farfar_124
ER  - 

@article{
author = "Milić, B and Nedeljković, M and Đukić, Mirjana",
year = "1995",
abstract = "A high-performance liquid chromatographic (HPLC) assay was developed to determine HI-6 and trimedoxime concentrations in small volumes of plasma. The HPLC system comprised a high-pressure pump (model 2150), a multiwavelenght detector (model 2140) set at 284 nm and 295 nm for detection of trimedoxime and HI-6, respectively, and a sample injector (Rheodyne, model 7125) with a 20 μL sample loop. A Superpac starter kit 5 μm ODS 2; 4 x 250 mm (Pharmacia LKB) stainless steel column was used. To plasma sample, 10 μL of 20% trichlorocetic acid, was added. From the supernatant, 20 μL was injected directly into HPLC. The solvent was 25% acetonitrile in 0.08 mol/L acetic acid (pH 3) containing 0.01 mol/L heptane-1-sulfonic acid (sodium salt) and 0.0025 mol/L tetramethylammonium chloride. The flow rate was 0.8 mL/min HI-6, and trimedoxime eluted at about 6.0 min and 8.0 min, respectively. The limit of sensitivity was 1.4 μmol/L of trimedoxime and 2.5 μmol/L of HI-6. Recovery rates were 99.02% in determination of HI-6 and 100.6% in determination of trimedoxime. Coefficients of variation for HI-6 and trimedoxime ranged from 1.4% to 8.2% and 0.9% to 3.4%, respectively. Our results indicate that the method is sensitive, accurate and precise.",
journal = "Jugoslovenska medicinska biohemija",
title = "HPLC method for determination of oximes HI-6 and trimedoxime in plasma, HPLC metoda za odredivanje oksima hi-6 i trimedoksima u plazmi",
volume = "14",
number = "3-4",
pages = "125-129",
url = "https://hdl.handle.net/21.15107/rcub_farfar_124"
}

Milić, B., Nedeljković, M.,& Đukić, M.. (1995). HPLC method for determination of oximes HI-6 and trimedoxime in plasma. in Jugoslovenska medicinska biohemija, 14(3-4), 125-129.
https://hdl.handle.net/21.15107/rcub_farfar_124

Milić B, Nedeljković M, Đukić M. HPLC method for determination of oximes HI-6 and trimedoxime in plasma. in Jugoslovenska medicinska biohemija. 1995;14(3-4):125-129.
https://hdl.handle.net/21.15107/rcub_farfar_124 .

Milić, B, Nedeljković, M, Đukić, Mirjana, "HPLC method for determination of oximes HI-6 and trimedoxime in plasma" in Jugoslovenska medicinska biohemija, 14, no. 3-4 (1995):125-129,
https://hdl.handle.net/21.15107/rcub_farfar_124 .