TY  - JOUR
AU  - Semenova, Irina
AU  - Bryskina, Diana
AU  - Cvetanović Kljakić, Aleksandra
AU  - Ražić, Slavica
AU  - Ananiev, Vasiliy
AU  - Rodin, Igor
AU  - Shpigun, Oleg
AU  - Stavrianidi, Andrey
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3133
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4115
AB  - Introduction: Limited availability of individual standards is a bottleneck for quality control of functional foods and natural medicines. The use of standard mixtures or secondary standards is a possible alternative in this case. Earlier, an approach known as standardised reference extract (RE) strategy was introduced for HPLC-UV analysis of different plant materials; however, its application in HPLC-MS analysis has not been investigated. Objective: To establish an HPLC-MS-based RE method for determination of ginsenoside content in ginseng infusions using commercially available extract refer- ence material of Panax quinquefolius L. Results: The developed HPLC-MS method was validated as precise (1.1%–9.4% intra-day variation; 1.6%–12.8% inter-day variation) and highly sensitive [limit of detection (LOD): 1–40 ng/mL; limit of quantification (LOQ): 4–120 ng/mL]. The sta- bility of samples was satisfactory (5.7%–16.3%). The RE quantification method was compared with the external standard method, and the obtained difference was not significant, mostly in the range of 5%–10%. Matrix effects for the diluted samples of RE and ginseng infusions, determined via the standard addition method, were in the range of 85%–115% and 80%–126%, respectively, and were also positively corre- lated with the ginsenoside concentration. Eleven batches of ginseng infusions from different manufacturers were analysed using the established method. Conclusion: The method for HPLC-MS-based ginsenoside quantification using RE as a secondary standard was established for the first time. The results of this study dem- onstrate that the application of the standardised RE strategy in HPLC-MS can mini- mise the matrix effect-related error in addition to the cost-effective quality control of herbal products, foods, and traditional medicines.
PB  - John Wiley and Sons Ltd
T2  - Phytochemical Analysis
T1  - An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection
VL  - 33
IS  - 6
SP  - 838
EP  - 850
DO  - 10.1002/pca.3133
ER  - 

@article{
author = "Semenova, Irina and Bryskina, Diana and Cvetanović Kljakić, Aleksandra and Ražić, Slavica and Ananiev, Vasiliy and Rodin, Igor and Shpigun, Oleg and Stavrianidi, Andrey",
year = "2022",
abstract = "Introduction: Limited availability of individual standards is a bottleneck for quality control of functional foods and natural medicines. The use of standard mixtures or secondary standards is a possible alternative in this case. Earlier, an approach known as standardised reference extract (RE) strategy was introduced for HPLC-UV analysis of different plant materials; however, its application in HPLC-MS analysis has not been investigated. Objective: To establish an HPLC-MS-based RE method for determination of ginsenoside content in ginseng infusions using commercially available extract refer- ence material of Panax quinquefolius L. Results: The developed HPLC-MS method was validated as precise (1.1%–9.4% intra-day variation; 1.6%–12.8% inter-day variation) and highly sensitive [limit of detection (LOD): 1–40 ng/mL; limit of quantification (LOQ): 4–120 ng/mL]. The sta- bility of samples was satisfactory (5.7%–16.3%). The RE quantification method was compared with the external standard method, and the obtained difference was not significant, mostly in the range of 5%–10%. Matrix effects for the diluted samples of RE and ginseng infusions, determined via the standard addition method, were in the range of 85%–115% and 80%–126%, respectively, and were also positively corre- lated with the ginsenoside concentration. Eleven batches of ginseng infusions from different manufacturers were analysed using the established method. Conclusion: The method for HPLC-MS-based ginsenoside quantification using RE as a secondary standard was established for the first time. The results of this study dem- onstrate that the application of the standardised RE strategy in HPLC-MS can mini- mise the matrix effect-related error in addition to the cost-effective quality control of herbal products, foods, and traditional medicines.",
publisher = "John Wiley and Sons Ltd",
journal = "Phytochemical Analysis",
title = "An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection",
volume = "33",
number = "6",
pages = "838-850",
doi = "10.1002/pca.3133"
}

Semenova, I., Bryskina, D., Cvetanović Kljakić, A., Ražić, S., Ananiev, V., Rodin, I., Shpigun, O.,& Stavrianidi, A.. (2022). An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection. in Phytochemical Analysis
John Wiley and Sons Ltd., 33(6), 838-850.
https://doi.org/10.1002/pca.3133

Semenova I, Bryskina D, Cvetanović Kljakić A, Ražić S, Ananiev V, Rodin I, Shpigun O, Stavrianidi A. An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection. in Phytochemical Analysis. 2022;33(6):838-850.
doi:10.1002/pca.3133 .

Semenova, Irina, Bryskina, Diana, Cvetanović Kljakić, Aleksandra, Ražić, Slavica, Ananiev, Vasiliy, Rodin, Igor, Shpigun, Oleg, Stavrianidi, Andrey, "An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection" in Phytochemical Analysis, 33, no. 6 (2022):838-850,
https://doi.org/10.1002/pca.3133 . .