TY  - JOUR
AU  - Božić, Dragana
AU  - Ćirković, Ivana
AU  - Milovanović, Jovica
AU  - Bufan, Biljana
AU  - Folić, Miljan
AU  - Savić Vujović, Katarina
AU  - Pavlović, Bojan
AU  - Jotić, Ana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5341
AB  - Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.
PB  - MDPI
T2  - Pharmaceuticals
T1  - In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections
VL  - 16
IS  - 11
DO  - 10.3390/ph16111604
ER  - 

@article{
author = "Božić, Dragana and Ćirković, Ivana and Milovanović, Jovica and Bufan, Biljana and Folić, Miljan and Savić Vujović, Katarina and Pavlović, Bojan and Jotić, Ana",
year = "2023",
abstract = "Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections",
volume = "16",
number = "11",
doi = "10.3390/ph16111604"
}

Božić, D., Ćirković, I., Milovanović, J., Bufan, B., Folić, M., Savić Vujović, K., Pavlović, B.,& Jotić, A.. (2023). In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections. in Pharmaceuticals
MDPI., 16(11).
https://doi.org/10.3390/ph16111604

Božić D, Ćirković I, Milovanović J, Bufan B, Folić M, Savić Vujović K, Pavlović B, Jotić A. In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections. in Pharmaceuticals. 2023;16(11).
doi:10.3390/ph16111604 .

Božić, Dragana, Ćirković, Ivana, Milovanović, Jovica, Bufan, Biljana, Folić, Miljan, Savić Vujović, Katarina, Pavlović, Bojan, Jotić, Ana, "In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections" in Pharmaceuticals, 16, no. 11 (2023),
https://doi.org/10.3390/ph16111604 . .

TY  - JOUR
AU  - Božić, Dragana
AU  - Pavlović, Bojan
AU  - Milovanović, Jovica
AU  - Jotić, Ana
AU  - Colović, Jelena
AU  - Cirković, Ivana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3212
AB  - Microbial biofilms have been implicated in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The aim of our study was to evaluate in vitro effects of amoxicillin-clavulanic acid and levofloxacin on biofilm formation by bacterial species isolated from sinus tissue in patients with CRSwNP. The sinus mucosal specimens were harvested from the upper parts and roof of ethmoid cavity of 48 patients with CRSwNP. Each sample was washed thoroughly in three separate beakers of sterile saline to remove any planktonic bacteria and further subjected to microbiology analysis. The biofilm-forming capacity of isolated strains was detected by microtiter-plate method and the effects of subinhibitory (1/2x to 1/16x MIC) and suprainhibitory concentrations (4, 8, 16, 32, and 64 A mu g/ml) of amoxicillin-clavulanic acid and levofloxacin on biofilm production were investigated. Bacterial strains were isolated in 42 (87.5%) patients: one microorganism in 80.9% and two microorganisms in 19.1% of patients. The most prevalent bacteria in CRSwNP biofilms were Staphylococcus epidermidis (34%) and S. aureus (28%) followed by S. haemolyticus (12%), Pseudomonas aeruginosa (8%), Moraxella catarrhalis (6%), Streptococcus pneumoniae (6%), and other staphylococci (6%). Subinhibitory concentrations of amoxicillin-clavulanic acid and levofloxacin significantly reduced biofilm formation (p  lt  0.01 and p  lt  0.05, respectively), with better efficacy of amoxicillin-clavulanic acid (1/2-1/8x MIC) on staphylococci and levofloxacin (1/2- 1/4x MIC) on M. catarrhalis and P. aeruginosa biofilm formation. Suprainhibitory concentrations of both tested antibiotics (4-64 A mu g/ml) significantly eradicated mature biofilms of staphylococci (p  lt  0.01). The effect of levofloxacin on eradication of staphylococcal biofilms was more noticeable, compared to the effect of amoxicillin-clavulanic acid (p  lt  0.01). Suprainhibitory concentrations of both tested antibiotics had no effect on eradication of previously formed M. catarrhalis and P. aeruginosa biofilms (p > 0.05). The amoxicillin-clavulanic acid and levofloxacin are shown to be potent antibiofilm agents in patients with CRSwNP. The effects of tested compounds depend on bacterial species and the volume of formed biofilm.
PB  - Springer, New York
T2  - European Archives of Oto-Rhino-Laryngology
T1  - Antibiofilm effects of amoxicillin-clavulanic acid and levofloxacin in patients with chronic rhinosinusitis with nasal polyposis
VL  - 275
IS  - 8
SP  - 2051
EP  - 2059
DO  - 10.1007/s00405-018-5049-6
ER  - 

@article{
author = "Božić, Dragana and Pavlović, Bojan and Milovanović, Jovica and Jotić, Ana and Colović, Jelena and Cirković, Ivana",
year = "2018",
abstract = "Microbial biofilms have been implicated in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The aim of our study was to evaluate in vitro effects of amoxicillin-clavulanic acid and levofloxacin on biofilm formation by bacterial species isolated from sinus tissue in patients with CRSwNP. The sinus mucosal specimens were harvested from the upper parts and roof of ethmoid cavity of 48 patients with CRSwNP. Each sample was washed thoroughly in three separate beakers of sterile saline to remove any planktonic bacteria and further subjected to microbiology analysis. The biofilm-forming capacity of isolated strains was detected by microtiter-plate method and the effects of subinhibitory (1/2x to 1/16x MIC) and suprainhibitory concentrations (4, 8, 16, 32, and 64 A mu g/ml) of amoxicillin-clavulanic acid and levofloxacin on biofilm production were investigated. Bacterial strains were isolated in 42 (87.5%) patients: one microorganism in 80.9% and two microorganisms in 19.1% of patients. The most prevalent bacteria in CRSwNP biofilms were Staphylococcus epidermidis (34%) and S. aureus (28%) followed by S. haemolyticus (12%), Pseudomonas aeruginosa (8%), Moraxella catarrhalis (6%), Streptococcus pneumoniae (6%), and other staphylococci (6%). Subinhibitory concentrations of amoxicillin-clavulanic acid and levofloxacin significantly reduced biofilm formation (p  lt  0.01 and p  lt  0.05, respectively), with better efficacy of amoxicillin-clavulanic acid (1/2-1/8x MIC) on staphylococci and levofloxacin (1/2- 1/4x MIC) on M. catarrhalis and P. aeruginosa biofilm formation. Suprainhibitory concentrations of both tested antibiotics (4-64 A mu g/ml) significantly eradicated mature biofilms of staphylococci (p  lt  0.01). The effect of levofloxacin on eradication of staphylococcal biofilms was more noticeable, compared to the effect of amoxicillin-clavulanic acid (p  lt  0.01). Suprainhibitory concentrations of both tested antibiotics had no effect on eradication of previously formed M. catarrhalis and P. aeruginosa biofilms (p > 0.05). The amoxicillin-clavulanic acid and levofloxacin are shown to be potent antibiofilm agents in patients with CRSwNP. The effects of tested compounds depend on bacterial species and the volume of formed biofilm.",
publisher = "Springer, New York",
journal = "European Archives of Oto-Rhino-Laryngology",
title = "Antibiofilm effects of amoxicillin-clavulanic acid and levofloxacin in patients with chronic rhinosinusitis with nasal polyposis",
volume = "275",
number = "8",
pages = "2051-2059",
doi = "10.1007/s00405-018-5049-6"
}

Božić, D., Pavlović, B., Milovanović, J., Jotić, A., Colović, J.,& Cirković, I.. (2018). Antibiofilm effects of amoxicillin-clavulanic acid and levofloxacin in patients with chronic rhinosinusitis with nasal polyposis. in European Archives of Oto-Rhino-Laryngology
Springer, New York., 275(8), 2051-2059.
https://doi.org/10.1007/s00405-018-5049-6

Božić D, Pavlović B, Milovanović J, Jotić A, Colović J, Cirković I. Antibiofilm effects of amoxicillin-clavulanic acid and levofloxacin in patients with chronic rhinosinusitis with nasal polyposis. in European Archives of Oto-Rhino-Laryngology. 2018;275(8):2051-2059.
doi:10.1007/s00405-018-5049-6 .

Božić, Dragana, Pavlović, Bojan, Milovanović, Jovica, Jotić, Ana, Colović, Jelena, Cirković, Ivana, "Antibiofilm effects of amoxicillin-clavulanic acid and levofloxacin in patients with chronic rhinosinusitis with nasal polyposis" in European Archives of Oto-Rhino-Laryngology, 275, no. 8 (2018):2051-2059,
https://doi.org/10.1007/s00405-018-5049-6 . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Pavlović, Bojan
AU  - Božić, Dragana
AU  - Jotić, Ana
AU  - Bakić, Ljubica
AU  - Milovanović, Jovica
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3005
AB  - Microbial biofilms have been implicated in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). Intranasal application of corticosteroids and saline is a reliable option for their management. The aim of our study was to evaluate in vitro antibiofilm effects of corticosteroids and isotonic and hypertonic nasal saline in CRSwNP patients. The sinus mucosal specimens were harvested from the ethmoid cavity of 48 patients with CRSwNP and further subjected to hematoxylin-eosin staining and microbiology analysis. The biofilm-forming capacity of isolated bacterial strains was detected by microtiter-plate method and the effects of therapeutic doses of mometasone, fluticasone, isotonic and hypertonic saline on biofilm production were investigated. Bacterial strains were isolated in 42 (87.5%) patients: one organism in 34 (80.9%) and two organisms in 8 (19.1%). Staphylococcus epidermidis (34%) and Staphylococcus aureus (28%) were the most prevalent bacteria in biofilms of CRSwNP patients. Corticosteroids and saline solutions significantly reduced biofilm formation (p  lt  0.01 and p  lt  0.05, respectively) with better efficacy of fluticasone and isotonic nasal saline. Treatment with fluticasone, mometasone, isotonic and hypertonic nasal saline completely prevented biofilm production in 66, 50, 84 and 38% of bacterial strains, respectively. The most significant density reduction was observed in biofilm formed by Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pneumoniae compared to other bacterial species (p  lt  0.01, p  lt  0.05, p  lt  0.05, respectively). The antibiofilm effects of corticosteroids and saline solutions also greatly depended on bacterial biomass (p  lt  0.05), with the most significant effect on high compared to small amount of formed biofilm. The topical steroids and nasal saline are shown to be potent antibiofilm agents in patients with CRSwNP. The effects of tested compounds depend on bacterial species and volume of formed biofilm.
PB  - Springer, New York
T2  - European Archives of Oto-Rhino-Laryngology
T1  - Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity
VL  - 274
IS  - 4
SP  - 1897
EP  - 1903
DO  - 10.1007/s00405-017-4454-6
ER  - 

@article{
author = "Cirković, Ivana and Pavlović, Bojan and Božić, Dragana and Jotić, Ana and Bakić, Ljubica and Milovanović, Jovica",
year = "2017",
abstract = "Microbial biofilms have been implicated in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). Intranasal application of corticosteroids and saline is a reliable option for their management. The aim of our study was to evaluate in vitro antibiofilm effects of corticosteroids and isotonic and hypertonic nasal saline in CRSwNP patients. The sinus mucosal specimens were harvested from the ethmoid cavity of 48 patients with CRSwNP and further subjected to hematoxylin-eosin staining and microbiology analysis. The biofilm-forming capacity of isolated bacterial strains was detected by microtiter-plate method and the effects of therapeutic doses of mometasone, fluticasone, isotonic and hypertonic saline on biofilm production were investigated. Bacterial strains were isolated in 42 (87.5%) patients: one organism in 34 (80.9%) and two organisms in 8 (19.1%). Staphylococcus epidermidis (34%) and Staphylococcus aureus (28%) were the most prevalent bacteria in biofilms of CRSwNP patients. Corticosteroids and saline solutions significantly reduced biofilm formation (p  lt  0.01 and p  lt  0.05, respectively) with better efficacy of fluticasone and isotonic nasal saline. Treatment with fluticasone, mometasone, isotonic and hypertonic nasal saline completely prevented biofilm production in 66, 50, 84 and 38% of bacterial strains, respectively. The most significant density reduction was observed in biofilm formed by Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pneumoniae compared to other bacterial species (p  lt  0.01, p  lt  0.05, p  lt  0.05, respectively). The antibiofilm effects of corticosteroids and saline solutions also greatly depended on bacterial biomass (p  lt  0.05), with the most significant effect on high compared to small amount of formed biofilm. The topical steroids and nasal saline are shown to be potent antibiofilm agents in patients with CRSwNP. The effects of tested compounds depend on bacterial species and volume of formed biofilm.",
publisher = "Springer, New York",
journal = "European Archives of Oto-Rhino-Laryngology",
title = "Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity",
volume = "274",
number = "4",
pages = "1897-1903",
doi = "10.1007/s00405-017-4454-6"
}

Cirković, I., Pavlović, B., Božić, D., Jotić, A., Bakić, L.,& Milovanović, J.. (2017). Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity. in European Archives of Oto-Rhino-Laryngology
Springer, New York., 274(4), 1897-1903.
https://doi.org/10.1007/s00405-017-4454-6

Cirković I, Pavlović B, Božić D, Jotić A, Bakić L, Milovanović J. Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity. in European Archives of Oto-Rhino-Laryngology. 2017;274(4):1897-1903.
doi:10.1007/s00405-017-4454-6 .

Cirković, Ivana, Pavlović, Bojan, Božić, Dragana, Jotić, Ana, Bakić, Ljubica, Milovanović, Jovica, "Antibiofilm effects of topical corticosteroids and intranasal saline in patients with chronic rhinosinusitis with nasal polyps depend on bacterial species and their biofilm-forming capacity" in European Archives of Oto-Rhino-Laryngology, 274, no. 4 (2017):1897-1903,
https://doi.org/10.1007/s00405-017-4454-6 . .

TY  - JOUR
AU  - Pavlović, Bojan
AU  - Božić, Dragana
AU  - Milovanović, Jovica
AU  - Jotić, Ana
AU  - Đukić, Vojko
AU  - Đukić, Slobodanka
AU  - Konstantinović, Neda
AU  - Cirković, Ivana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2695
AB  - Objectives: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. Methods: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations. Results: All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p  lt  0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p  lt  0.05). Conclusion: Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Quantification of biofilm formation on silicone intranasal splints: An in vitro study
VL  - 63
IS  - 3
SP  - 301
EP  - 311
DO  - 10.1556/030.63.2016.006
ER  - 

@article{
author = "Pavlović, Bojan and Božić, Dragana and Milovanović, Jovica and Jotić, Ana and Đukić, Vojko and Đukić, Slobodanka and Konstantinović, Neda and Cirković, Ivana",
year = "2016",
abstract = "Objectives: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. Methods: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations. Results: All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p  lt  0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p  lt  0.05). Conclusion: Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Quantification of biofilm formation on silicone intranasal splints: An in vitro study",
volume = "63",
number = "3",
pages = "301-311",
doi = "10.1556/030.63.2016.006"
}

Pavlović, B., Božić, D., Milovanović, J., Jotić, A., Đukić, V., Đukić, S., Konstantinović, N.,& Cirković, I.. (2016). Quantification of biofilm formation on silicone intranasal splints: An in vitro study. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 63(3), 301-311.
https://doi.org/10.1556/030.63.2016.006

Pavlović B, Božić D, Milovanović J, Jotić A, Đukić V, Đukić S, Konstantinović N, Cirković I. Quantification of biofilm formation on silicone intranasal splints: An in vitro study. in Acta Microbiologica et Immunologica Hungarica. 2016;63(3):301-311.
doi:10.1556/030.63.2016.006 .

Pavlović, Bojan, Božić, Dragana, Milovanović, Jovica, Jotić, Ana, Đukić, Vojko, Đukić, Slobodanka, Konstantinović, Neda, Cirković, Ivana, "Quantification of biofilm formation on silicone intranasal splints: An in vitro study" in Acta Microbiologica et Immunologica Hungarica, 63, no. 3 (2016):301-311,
https://doi.org/10.1556/030.63.2016.006 . .

TY  - JOUR
AU  - Jotić, Ana
AU  - Božić, Dragana
AU  - Milovanović, Jovica
AU  - Pavlović, Bojan
AU  - Jesić, Snežana
AU  - Pelemis, Mijomir
AU  - Novaković, Marko
AU  - Cirković, Ivana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2561
AB  - Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p  lt  0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.
PB  - Springer, New York
T2  - European Archives of Oto-Rhino-Laryngology
T1  - Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage
VL  - 273
IS  - 3
SP  - 615
EP  - 620
DO  - 10.1007/s00405-015-3607-8
ER  - 

@article{
author = "Jotić, Ana and Božić, Dragana and Milovanović, Jovica and Pavlović, Bojan and Jesić, Snežana and Pelemis, Mijomir and Novaković, Marko and Cirković, Ivana",
year = "2016",
abstract = "Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p  lt  0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.",
publisher = "Springer, New York",
journal = "European Archives of Oto-Rhino-Laryngology",
title = "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage",
volume = "273",
number = "3",
pages = "615-620",
doi = "10.1007/s00405-015-3607-8"
}

Jotić, A., Božić, D., Milovanović, J., Pavlović, B., Jesić, S., Pelemis, M., Novaković, M.,& Cirković, I.. (2016). Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology
Springer, New York., 273(3), 615-620.
https://doi.org/10.1007/s00405-015-3607-8

Jotić A, Božić D, Milovanović J, Pavlović B, Jesić S, Pelemis M, Novaković M, Cirković I. Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology. 2016;273(3):615-620.
doi:10.1007/s00405-015-3607-8 .

Jotić, Ana, Božić, Dragana, Milovanović, Jovica, Pavlović, Bojan, Jesić, Snežana, Pelemis, Mijomir, Novaković, Marko, Cirković, Ivana, "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage" in European Archives of Oto-Rhino-Laryngology, 273, no. 3 (2016):615-620,
https://doi.org/10.1007/s00405-015-3607-8 . .