@article{
author = "Varga, Filip and Liber, Zlatko and Turudić, Ante and Jakše, Jernej and Juzbašić, Lea and Jeran, Nina and Grdiša, Martina and Zbiljić, Miloš and Šatović, Zlatko",
year = "2024",
abstract = "Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution
in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid
species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo
assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum
assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying
different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and
after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles
were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis
of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum
than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and
genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted
exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae
individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis
of genetic diversity and structure, as well as molecular identification of their putative hybrids in
the future.",
publisher = "MDPI",
journal = "Diversity",
title = "Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid",
volume = "16",
number = "4",
pages = "204",
doi = "10.3390/d16040206"
}
