TY  - CONF
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brankov, Jovan
AU  - Zhou, Wei
AU  - Brey, Eric
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5608
AB  - INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.
PB  - Savez hemijskih inženjera, Beograd
C3  - Hemijska industrija
T1  - Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay
VL  - 78
IS  - 1S
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5608
ER  - 

@conference{
author = "Živković, Lada and Spremo-Potparević, Biljana and Bajić, Vladan and Brankov, Jovan and Zhou, Wei and Brey, Eric",
year = "2024",
abstract = "INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay",
volume = "78",
number = "1S",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5608"
}

Živković, L., Spremo-Potparević, B., Bajić, V., Brankov, J., Zhou, W.,& Brey, E.. (2024). Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 78(1S), 14-14.
https://doi.org/10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608

Živković L, Spremo-Potparević B, Bajić V, Brankov J, Zhou W, Brey E. Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija. 2024;78(1S):14-14.
doi:10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

Živković, Lada, Spremo-Potparević, Biljana, Bajić, Vladan, Brankov, Jovan, Zhou, Wei, Brey, Eric, "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay" in Hemijska industrija, 78, no. 1S (2024):14-14,
https://doi.org/10.1016/j.reprotox.2024.108585 .,
https://hdl.handle.net/21.15107/rcub_farfar_5608 .