TY  - CONF
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brankov, Jovan
AU  - Zhou, Wei
AU  - Brey, Eric
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5608
AB  - INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.
PB  - Savez hemijskih inženjera, Beograd
C3  - Hemijska industrija
T1  - Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay
VL  - 78
IS  - 1S
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5608
ER  - 

@conference{
author = "Živković, Lada and Spremo-Potparević, Biljana and Bajić, Vladan and Brankov, Jovan and Zhou, Wei and Brey, Eric",
year = "2024",
abstract = "INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay",
volume = "78",
number = "1S",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5608"
}

Živković, L., Spremo-Potparević, B., Bajić, V., Brankov, J., Zhou, W.,& Brey, E.. (2024). Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 78(1S), 14-14.
https://doi.org/10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608

Živković L, Spremo-Potparević B, Bajić V, Brankov J, Zhou W, Brey E. Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay. in Hemijska industrija. 2024;78(1S):14-14.
doi:10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

Živković, Lada, Spremo-Potparević, Biljana, Bajić, Vladan, Brankov, Jovan, Zhou, Wei, Brey, Eric, "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay" in Hemijska industrija, 78, no. 1S (2024):14-14,
https://doi.org/10.1016/j.reprotox.2024.108585 .,
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

TY  - JOUR
AU  - Živković, Lada
AU  - Akar, Banu
AU  - Roux, Brianna M.
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brey, Eric M.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2934
AB  - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
VL  - 105
IS  - 1
SP  - 284
EP  - 291
DO  - 10.1002/jbm.a.35849
ER  - 

@article{
author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Potparević, Biljana and Bajić, Vladan and Brey, Eric M.",
year = "2017",
abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres",
volume = "105",
number = "1",
pages = "284-291",
doi = "10.1002/jbm.a.35849"
}

Živković, L., Akar, B., Roux, B. M., Potparević, B., Bajić, V.,& Brey, E. M.. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research Part A
Wiley-Blackwell, Hoboken., 105(1), 284-291.
https://doi.org/10.1002/jbm.a.35849

Živković L, Akar B, Roux BM, Potparević B, Bajić V, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research Part A. 2017;105(1):284-291.
doi:10.1002/jbm.a.35849 .

Živković, Lada, Akar, Banu, Roux, Brianna M., Potparević, Biljana, Bajić, Vladan, Brey, Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" in Journal of Biomedical Materials Research Part A, 105, no. 1 (2017):284-291,
https://doi.org/10.1002/jbm.a.35849 . .

TY  - JOUR
AU  - Gandhi, Jarel K.
AU  - Živković, Lada
AU  - Fisher, John P.
AU  - Yoder, Mervin C.
AU  - Brey, Eric M.
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2328
AB  - Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.
PB  - MDPI, Basel
T2  - Separation Science and Technology
T1  - Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization
VL  - 15
IS  - 9
SP  - 23886
EP  - 23902
DO  - 10.3390/s150923886
ER  - 

@article{
author = "Gandhi, Jarel K. and Živković, Lada and Fisher, John P. and Yoder, Mervin C. and Brey, Eric M.",
year = "2015",
abstract = "Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.",
publisher = "MDPI, Basel",
journal = "Separation Science and Technology",
title = "Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization",
volume = "15",
number = "9",
pages = "23886-23902",
doi = "10.3390/s150923886"
}

Gandhi, J. K., Živković, L., Fisher, J. P., Yoder, M. C.,& Brey, E. M.. (2015). Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization. in Separation Science and Technology
MDPI, Basel., 15(9), 23886-23902.
https://doi.org/10.3390/s150923886

Gandhi JK, Živković L, Fisher JP, Yoder MC, Brey EM. Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization. in Separation Science and Technology. 2015;15(9):23886-23902.
doi:10.3390/s150923886 .

Gandhi, Jarel K., Živković, Lada, Fisher, John P., Yoder, Mervin C., Brey, Eric M., "Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization" in Separation Science and Technology, 15, no. 9 (2015):23886-23902,
https://doi.org/10.3390/s150923886 . .