TY  - JOUR
AU  - Stanković, Milena N.
AU  - Mladenović, Dušan
AU  - Đuričić, Ivana
AU  - Šobajić, Slađana
AU  - Timić, Jasmina
AU  - Jorgačević, Bojan
AU  - Aleksić, Vuk
AU  - Vucević, Danijela B.
AU  - Jesić-Vukicević, Rada
AU  - Radosavljević, Tatjana S.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2153
AB  - Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.
PB  - Elsevier Science Inc, New York
T2  - Archives of Medical Research
T1  - Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease
VL  - 45
IS  - 2
SP  - 116
EP  - 124
DO  - 10.1016/j.arcmed.2013.12.010
ER  - 

@article{
author = "Stanković, Milena N. and Mladenović, Dušan and Đuričić, Ivana and Šobajić, Slađana and Timić, Jasmina and Jorgačević, Bojan and Aleksić, Vuk and Vucević, Danijela B. and Jesić-Vukicević, Rada and Radosavljević, Tatjana S.",
year = "2014",
abstract = "Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Medical Research",
title = "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease",
volume = "45",
number = "2",
pages = "116-124",
doi = "10.1016/j.arcmed.2013.12.010"
}

Stanković, M. N., Mladenović, D., Đuričić, I., Šobajić, S., Timić, J., Jorgačević, B., Aleksić, V., Vucević, D. B., Jesić-Vukicević, R.,& Radosavljević, T. S.. (2014). Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease. in Archives of Medical Research
Elsevier Science Inc, New York., 45(2), 116-124.
https://doi.org/10.1016/j.arcmed.2013.12.010

Stanković MN, Mladenović D, Đuričić I, Šobajić S, Timić J, Jorgačević B, Aleksić V, Vucević DB, Jesić-Vukicević R, Radosavljević TS. Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease. in Archives of Medical Research. 2014;45(2):116-124.
doi:10.1016/j.arcmed.2013.12.010 .

Stanković, Milena N., Mladenović, Dušan, Đuričić, Ivana, Šobajić, Slađana, Timić, Jasmina, Jorgačević, Bojan, Aleksić, Vuk, Vucević, Danijela B., Jesić-Vukicević, Rada, Radosavljević, Tatjana S., "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease" in Archives of Medical Research, 45, no. 2 (2014):116-124,
https://doi.org/10.1016/j.arcmed.2013.12.010 . .