TY  - JOUR
AU  - Clayton, Terry
AU  - Poe, Michael M.
AU  - Rallapalli, Sundari
AU  - Biawat, Poonam
AU  - Savić, Miroslav
AU  - Rowlett, James K.
AU  - Gallos, George
AU  - Emala, Charles
AU  - Kaczorowski, Catherine C.
AU  - Stafford, Douglas C.
AU  - Arnold, Leggy
AU  - Cook, James M.
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2317
AB  - An updated model of the GABA(A) benzodiazepine receptor pharmacophore of the alpha 5-BzR/GABA(A) subtype has been constructed prompted by the synthesis of subtype selective ligands in light of the recent developments in both ligand synthesis, behavioral studies, and molecular modeling studies of the binding site itself. A number of BzR/GABA(A) alpha 5 subtype selective compounds were synthesized, notably alpha 5-subtype selective inverse agonist PWZ-029 (1) which is active in enhancing cognition in both rodents and primates. In addition, a chiral positive allosteric modulator (PAM), SH-053-2'F-R-CH3 (2), has been shown to reverse the deleterious effects in the MAM-model of schizophrenia as well as alleviate constriction in airway smooth muscle. Presented here is an updated model of the pharmacophore for alpha 5 beta 2 gamma 2 Bz/GABA(A) receptors, including a rendering of PWZ-029 docked within the alpha 5-binding pocket showing specific interactions of the molecule with the receptor. Differences in the included volume as compared to alpha 1 beta 2 gamma 2, alpha 2 beta 2 gamma 2, and alpha 3 beta 2 gamma 2 will be illustrated for clarity. These new models enhance the ability to understand structural characteristics of ligands which act as agonists, antagonists, or inverse agonists at the Bz BS of GABA(A) receptors.
PB  - Hindawi Ltd, London
T2  - International Journal of Medicinal Chemistry
T1  - A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model
DO  - 10.1155/2015/430248
ER  - 

@article{
author = "Clayton, Terry and Poe, Michael M. and Rallapalli, Sundari and Biawat, Poonam and Savić, Miroslav and Rowlett, James K. and Gallos, George and Emala, Charles and Kaczorowski, Catherine C. and Stafford, Douglas C. and Arnold, Leggy and Cook, James M.",
year = "2015",
abstract = "An updated model of the GABA(A) benzodiazepine receptor pharmacophore of the alpha 5-BzR/GABA(A) subtype has been constructed prompted by the synthesis of subtype selective ligands in light of the recent developments in both ligand synthesis, behavioral studies, and molecular modeling studies of the binding site itself. A number of BzR/GABA(A) alpha 5 subtype selective compounds were synthesized, notably alpha 5-subtype selective inverse agonist PWZ-029 (1) which is active in enhancing cognition in both rodents and primates. In addition, a chiral positive allosteric modulator (PAM), SH-053-2'F-R-CH3 (2), has been shown to reverse the deleterious effects in the MAM-model of schizophrenia as well as alleviate constriction in airway smooth muscle. Presented here is an updated model of the pharmacophore for alpha 5 beta 2 gamma 2 Bz/GABA(A) receptors, including a rendering of PWZ-029 docked within the alpha 5-binding pocket showing specific interactions of the molecule with the receptor. Differences in the included volume as compared to alpha 1 beta 2 gamma 2, alpha 2 beta 2 gamma 2, and alpha 3 beta 2 gamma 2 will be illustrated for clarity. These new models enhance the ability to understand structural characteristics of ligands which act as agonists, antagonists, or inverse agonists at the Bz BS of GABA(A) receptors.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Medicinal Chemistry",
title = "A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model",
doi = "10.1155/2015/430248"
}

Clayton, T., Poe, M. M., Rallapalli, S., Biawat, P., Savić, M., Rowlett, J. K., Gallos, G., Emala, C., Kaczorowski, C. C., Stafford, D. C., Arnold, L.,& Cook, J. M.. (2015). A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model. in International Journal of Medicinal Chemistry
Hindawi Ltd, London..
https://doi.org/10.1155/2015/430248

Clayton T, Poe MM, Rallapalli S, Biawat P, Savić M, Rowlett JK, Gallos G, Emala C, Kaczorowski CC, Stafford DC, Arnold L, Cook JM. A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model. in International Journal of Medicinal Chemistry. 2015;.
doi:10.1155/2015/430248 .

Clayton, Terry, Poe, Michael M., Rallapalli, Sundari, Biawat, Poonam, Savić, Miroslav, Rowlett, James K., Gallos, George, Emala, Charles, Kaczorowski, Catherine C., Stafford, Douglas C., Arnold, Leggy, Cook, James M., "A Review of the Updated Pharmacophore for the Alpha 5 GABA(A) Benzodiazepine Receptor Model" in International Journal of Medicinal Chemistry (2015),
https://doi.org/10.1155/2015/430248 . .

TY  - JOUR
AU  - Milić, Marija
AU  - Timić, Tamara
AU  - Joksimović, Srđan
AU  - Biawat, Poonam
AU  - Rallapalli, Sundari
AU  - Divljaković, Jovana
AU  - Radulović, Tamara
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1926
AB  - Inverse agonism at the benzodiazepine site of alpha(5) subunit-containing GABA(A) receptors is an attractive approach for the development of putative cognition-enhancing compounds, which are still far from clinical application. Several ligands with binding and/or functional selectivity for alpha(5) GABA(A) receptors have been synthesized and tested in a few animal models. PWZ-029 is an alpha(5) GABA(A) selective inverse agonist whose memory enhancing effects were demonstrated in the passive avoidance task in rats and in Pavlovian fear conditioning in mice. In the present study we investigated the effects of PWZ-029 administration in novel object recognition test and Morris water maze, in normal and scopolamine-treated rats. All the three doses of PWZ-029 (2,5 and 10 mg/kg) improved object recognition after the 24-h delay period, as shown by significant differences between the exploration times of the novel and old object, and the respective discrimination indices. PWZ-029 (2 mg/kg) also successfully reversed the 0.3 mg/kg scopolamine-induced deficit in recognition memory after the 1-h delay. In the Morris water maze test, PWZ-029 (5,10 and 15 mg/kg) did not significantly influence swim patterns, either during five acquisition days or during the treatment-free probe trial. PWZ-029 (2, 5 and 10 mg/kg) also proved to be ineffective in the reversal of the 1 mg/kg scopolamine-induced memory impairment in the water maze. The present mixed results encourage use of a variety of tests and experimental conditions in order to increase the predictability of preclinical testing of selective as GABA(A) inverse agonists.
PB  - Elsevier Science BV, Amsterdam
T2  - Behavioural Brain Research
T1  - PWZ-029, an inverse agonist selective for alpha(5) GABA(A) receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats
VL  - 241
SP  - 206
EP  - 213
DO  - 10.1016/j.bbr.2012.12.016
ER  - 

@article{
author = "Milić, Marija and Timić, Tamara and Joksimović, Srđan and Biawat, Poonam and Rallapalli, Sundari and Divljaković, Jovana and Radulović, Tamara and Cook, James M. and Savić, Miroslav",
year = "2013",
abstract = "Inverse agonism at the benzodiazepine site of alpha(5) subunit-containing GABA(A) receptors is an attractive approach for the development of putative cognition-enhancing compounds, which are still far from clinical application. Several ligands with binding and/or functional selectivity for alpha(5) GABA(A) receptors have been synthesized and tested in a few animal models. PWZ-029 is an alpha(5) GABA(A) selective inverse agonist whose memory enhancing effects were demonstrated in the passive avoidance task in rats and in Pavlovian fear conditioning in mice. In the present study we investigated the effects of PWZ-029 administration in novel object recognition test and Morris water maze, in normal and scopolamine-treated rats. All the three doses of PWZ-029 (2,5 and 10 mg/kg) improved object recognition after the 24-h delay period, as shown by significant differences between the exploration times of the novel and old object, and the respective discrimination indices. PWZ-029 (2 mg/kg) also successfully reversed the 0.3 mg/kg scopolamine-induced deficit in recognition memory after the 1-h delay. In the Morris water maze test, PWZ-029 (5,10 and 15 mg/kg) did not significantly influence swim patterns, either during five acquisition days or during the treatment-free probe trial. PWZ-029 (2, 5 and 10 mg/kg) also proved to be ineffective in the reversal of the 1 mg/kg scopolamine-induced memory impairment in the water maze. The present mixed results encourage use of a variety of tests and experimental conditions in order to increase the predictability of preclinical testing of selective as GABA(A) inverse agonists.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Behavioural Brain Research",
title = "PWZ-029, an inverse agonist selective for alpha(5) GABA(A) receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats",
volume = "241",
pages = "206-213",
doi = "10.1016/j.bbr.2012.12.016"
}

Milić, M., Timić, T., Joksimović, S., Biawat, P., Rallapalli, S., Divljaković, J., Radulović, T., Cook, J. M.,& Savić, M.. (2013). PWZ-029, an inverse agonist selective for alpha(5) GABA(A) receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats. in Behavioural Brain Research
Elsevier Science BV, Amsterdam., 241, 206-213.
https://doi.org/10.1016/j.bbr.2012.12.016

Milić M, Timić T, Joksimović S, Biawat P, Rallapalli S, Divljaković J, Radulović T, Cook JM, Savić M. PWZ-029, an inverse agonist selective for alpha(5) GABA(A) receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats. in Behavioural Brain Research. 2013;241:206-213.
doi:10.1016/j.bbr.2012.12.016 .

Milić, Marija, Timić, Tamara, Joksimović, Srđan, Biawat, Poonam, Rallapalli, Sundari, Divljaković, Jovana, Radulović, Tamara, Cook, James M., Savić, Miroslav, "PWZ-029, an inverse agonist selective for alpha(5) GABA(A) receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats" in Behavioural Brain Research, 241 (2013):206-213,
https://doi.org/10.1016/j.bbr.2012.12.016 . .

TY  - JOUR
AU  - Joksimović, Srđan
AU  - Varagić, Zdravko
AU  - Kovacević, Jovana
AU  - van Linn, Michael
AU  - Milić, Marija
AU  - Rallapalli, Sundari
AU  - Timić, Tamara
AU  - Sieghart, Werner
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1839
AB  - Synthesis of ligands inactive or with low activity at alpha(1) GABA(A) receptors has become the key concept for development of novel, more tolerable benzodiazepine (BZ)-like drugs. WYS8, a remarkably (105 times) alpha(1)-subtype selective partial positive modulator, may serve as a pharmacological tool for refining the role of alpha(1) GABA(A) receptors in mediation of BZs' effects. Here, the effects of WYS8 on GABA-induced currents and on diazepam-induced potentiation of recombinant BZ-sensitive GABA(A) receptors were studied in more detail. In addition, the behavioral profile of WYS8 (0.2, 1, and 10 mg/kg i.p.), on its own and in combination with diazepam, was tested in the spontaneous locomotor activity, elevated plus maze, grip strength, rotarod, and pentylenetetrazole tests. WYS8, applied at an in vivo attainable concentration of 100 nM, reduced the stimulation of GABA currents by 1 mu M diazepam by 57 % at alpha(1)beta(3)gamma(2), but not at alpha(2)beta(3)gamma(2), alpha(3)beta(3)gamma(2), or alpha(5)beta(3)gamma(2) GABA(A) receptors. The administration of WYS8 alone induced negligible behavioral consequences. When combined with diazepam, WYS8 caused a reduction in sedation, muscle relaxation, and anticonvulsant activity, as compared with this BZ alone, whereas ataxia was preserved, and the anxiolytic effect of 2 mg/kg diazepam was unmasked. Hence, a partial instead of full activation at alpha(1) GABA(A) receptors did not necessarily result in the attenuation of the effects assumed to be mediated by activation of these receptors, or in the full preservation of the effects mediated by activation of other GABA(A) receptors. Thus, the role of alpha(1) GABA(A) receptors appears more complex than that proposed by genetic studies.
PB  - Springer, New York
T2  - QSAR & Combinatorial Science
T1  - Insights into functional pharmacology of alpha(1) GABA(A) receptors: how much does partial activation at the benzodiazepine site matter?
VL  - 230
IS  - 1
SP  - 113
EP  - 123
DO  - 10.1007/s00213-013-3143-4
ER  - 

@article{
author = "Joksimović, Srđan and Varagić, Zdravko and Kovacević, Jovana and van Linn, Michael and Milić, Marija and Rallapalli, Sundari and Timić, Tamara and Sieghart, Werner and Cook, James M. and Savić, Miroslav",
year = "2013",
abstract = "Synthesis of ligands inactive or with low activity at alpha(1) GABA(A) receptors has become the key concept for development of novel, more tolerable benzodiazepine (BZ)-like drugs. WYS8, a remarkably (105 times) alpha(1)-subtype selective partial positive modulator, may serve as a pharmacological tool for refining the role of alpha(1) GABA(A) receptors in mediation of BZs' effects. Here, the effects of WYS8 on GABA-induced currents and on diazepam-induced potentiation of recombinant BZ-sensitive GABA(A) receptors were studied in more detail. In addition, the behavioral profile of WYS8 (0.2, 1, and 10 mg/kg i.p.), on its own and in combination with diazepam, was tested in the spontaneous locomotor activity, elevated plus maze, grip strength, rotarod, and pentylenetetrazole tests. WYS8, applied at an in vivo attainable concentration of 100 nM, reduced the stimulation of GABA currents by 1 mu M diazepam by 57 % at alpha(1)beta(3)gamma(2), but not at alpha(2)beta(3)gamma(2), alpha(3)beta(3)gamma(2), or alpha(5)beta(3)gamma(2) GABA(A) receptors. The administration of WYS8 alone induced negligible behavioral consequences. When combined with diazepam, WYS8 caused a reduction in sedation, muscle relaxation, and anticonvulsant activity, as compared with this BZ alone, whereas ataxia was preserved, and the anxiolytic effect of 2 mg/kg diazepam was unmasked. Hence, a partial instead of full activation at alpha(1) GABA(A) receptors did not necessarily result in the attenuation of the effects assumed to be mediated by activation of these receptors, or in the full preservation of the effects mediated by activation of other GABA(A) receptors. Thus, the role of alpha(1) GABA(A) receptors appears more complex than that proposed by genetic studies.",
publisher = "Springer, New York",
journal = "QSAR & Combinatorial Science",
title = "Insights into functional pharmacology of alpha(1) GABA(A) receptors: how much does partial activation at the benzodiazepine site matter?",
volume = "230",
number = "1",
pages = "113-123",
doi = "10.1007/s00213-013-3143-4"
}

Joksimović, S., Varagić, Z., Kovacević, J., van Linn, M., Milić, M., Rallapalli, S., Timić, T., Sieghart, W., Cook, J. M.,& Savić, M.. (2013). Insights into functional pharmacology of alpha(1) GABA(A) receptors: how much does partial activation at the benzodiazepine site matter?. in QSAR & Combinatorial Science
Springer, New York., 230(1), 113-123.
https://doi.org/10.1007/s00213-013-3143-4

Joksimović S, Varagić Z, Kovacević J, van Linn M, Milić M, Rallapalli S, Timić T, Sieghart W, Cook JM, Savić M. Insights into functional pharmacology of alpha(1) GABA(A) receptors: how much does partial activation at the benzodiazepine site matter?. in QSAR & Combinatorial Science. 2013;230(1):113-123.
doi:10.1007/s00213-013-3143-4 .

Joksimović, Srđan, Varagić, Zdravko, Kovacević, Jovana, van Linn, Michael, Milić, Marija, Rallapalli, Sundari, Timić, Tamara, Sieghart, Werner, Cook, James M., Savić, Miroslav, "Insights into functional pharmacology of alpha(1) GABA(A) receptors: how much does partial activation at the benzodiazepine site matter?" in QSAR & Combinatorial Science, 230, no. 1 (2013):113-123,
https://doi.org/10.1007/s00213-013-3143-4 . .

TY  - CONF
AU  - Milinković, Marija M.
AU  - Timić, Tamara
AU  - Divljaković, Jovana
AU  - Joksimović, Srđan
AU  - Rallapalli, Sundari
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1660
AB  - Objective : The impairing effects of diazepam in Morris water maze
(MWM) could be partially antagonized with co-administration of an
a5 subunit selective antagonist XLi093 (Savic ́ et al., 2009). In order
to further assess the role of the a5GABAA receptors population
in mediating amnesic effects in rats, the present study examined
effects of an a5GABAA selective agonist XLi356 on the MWM
performance.
Methods : Male Wistar rats were given vehicle or 5, 10 and 20 mg/
kg of XLI356 intraperitoneally 20 minutes before the testing. A single-
day water maze task had three swimming blocks, each consisting of
4 trials, lasting a maximum time of 60 s each. Afterwards, a probe trial
was given and a number of standard parameters was calculated.
Additionally, rats were tested in spontaneous locomotor activity
(SLA) and elevated plus maze (EPM) tests, where the sedative and
anxiolytic effects were assessed.
Results : Results were analyzed using one-way ANOVA with post
hoc Student-Newman-Keuls test where applicable. XLi356 signifi-
cantly increased latency to platform (F(3,444)=3.1287, p=0.026) ; post
hoc test revealed that the dose of 20 mg/kg was significantly different
from vehicle. The same dose of XLi356 significantly increased cumu-
lative distance from the platform zone (p=0.028) and the time spent
in the periphery ring (p=0.009), while the path efficiency was on the
control level. On the other hand, XLi356 did not show behavioral
activity in SLA and EPM tests at either of three doses tested.
Conclusion : The present results suggest that ligands with ap-
preciable agonist activity at GABAA receptors containing a5 subunits
may impair memory acquisition in Morris water maze task, without
discernible effects on general behavior. Thus the activity of the ben-
zodiazepine type drugs at a5GABAA receptors should be decreased if
the amnesic effects are to avoid.
PB  - Oxford Univ Press, Oxford
C3  - International Journal of Neuropsychopharmacology
T1  - The influence of an a5GABAA selective agonist XLi356 on rats' performance in morris water maze
VL  - 15
IS  - Supplement 1
SP  - 231
EP  - 231
DO  - 10.1017/S1461145712000508
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1660
ER  - 

@conference{
author = "Milinković, Marija M. and Timić, Tamara and Divljaković, Jovana and Joksimović, Srđan and Rallapalli, Sundari and Cook, James M. and Savić, Miroslav",
year = "2012",
abstract = "Objective : The impairing effects of diazepam in Morris water maze
(MWM) could be partially antagonized with co-administration of an
a5 subunit selective antagonist XLi093 (Savic ́ et al., 2009). In order
to further assess the role of the a5GABAA receptors population
in mediating amnesic effects in rats, the present study examined
effects of an a5GABAA selective agonist XLi356 on the MWM
performance.
Methods : Male Wistar rats were given vehicle or 5, 10 and 20 mg/
kg of XLI356 intraperitoneally 20 minutes before the testing. A single-
day water maze task had three swimming blocks, each consisting of
4 trials, lasting a maximum time of 60 s each. Afterwards, a probe trial
was given and a number of standard parameters was calculated.
Additionally, rats were tested in spontaneous locomotor activity
(SLA) and elevated plus maze (EPM) tests, where the sedative and
anxiolytic effects were assessed.
Results : Results were analyzed using one-way ANOVA with post
hoc Student-Newman-Keuls test where applicable. XLi356 signifi-
cantly increased latency to platform (F(3,444)=3.1287, p=0.026) ; post
hoc test revealed that the dose of 20 mg/kg was significantly different
from vehicle. The same dose of XLi356 significantly increased cumu-
lative distance from the platform zone (p=0.028) and the time spent
in the periphery ring (p=0.009), while the path efficiency was on the
control level. On the other hand, XLi356 did not show behavioral
activity in SLA and EPM tests at either of three doses tested.
Conclusion : The present results suggest that ligands with ap-
preciable agonist activity at GABAA receptors containing a5 subunits
may impair memory acquisition in Morris water maze task, without
discernible effects on general behavior. Thus the activity of the ben-
zodiazepine type drugs at a5GABAA receptors should be decreased if
the amnesic effects are to avoid.",
publisher = "Oxford Univ Press, Oxford",
journal = "International Journal of Neuropsychopharmacology",
title = "The influence of an a5GABAA selective agonist XLi356 on rats' performance in morris water maze",
volume = "15",
number = "Supplement 1",
pages = "231-231",
doi = "10.1017/S1461145712000508",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1660"
}

Milinković, M. M., Timić, T., Divljaković, J., Joksimović, S., Rallapalli, S., Cook, J. M.,& Savić, M.. (2012). The influence of an a5GABAA selective agonist XLi356 on rats' performance in morris water maze. in International Journal of Neuropsychopharmacology
Oxford Univ Press, Oxford., 15(Supplement 1), 231-231.
https://doi.org/10.1017/S1461145712000508
https://hdl.handle.net/21.15107/rcub_farfar_1660

Milinković MM, Timić T, Divljaković J, Joksimović S, Rallapalli S, Cook JM, Savić M. The influence of an a5GABAA selective agonist XLi356 on rats' performance in morris water maze. in International Journal of Neuropsychopharmacology. 2012;15(Supplement 1):231-231.
doi:10.1017/S1461145712000508
https://hdl.handle.net/21.15107/rcub_farfar_1660 .

Milinković, Marija M., Timić, Tamara, Divljaković, Jovana, Joksimović, Srđan, Rallapalli, Sundari, Cook, James M., Savić, Miroslav, "The influence of an a5GABAA selective agonist XLi356 on rats' performance in morris water maze" in International Journal of Neuropsychopharmacology, 15, no. Supplement 1 (2012):231-231,
https://doi.org/10.1017/S1461145712000508 .,
https://hdl.handle.net/21.15107/rcub_farfar_1660 .

TY  - CONF
AU  - Timić, Tamara
AU  - Divljaković, Jovana
AU  - Milinković, Marija M.
AU  - Rallapalli, Sundari
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1685
AB  - Objective : It is well known that benzodiazepine binding site ligands
influence learning and memory and that the a5 subunit is significantly
involved in cognition enhancement mediated by the negative modu-
lation of GABAA receptor function. PWZ-029, a moderately selective
a5GABA A receptor inverse agonist, improved learning in passive but
not in active avoidance test, without effects on anxiety or muscle tone.
The aim of this study was to investigate effects of PWZ-029 and
DMCM, a non-selective inverse agonist, on learning ability and short-
term memory in Morris water-maze (MWM) test.
Methods : MWM test was conducted 20 minutes after in-
traperitoneal administration of treatments (solvent, 5, 15 or 30 mg/kg
PWZ-029 or 2 mg/kg DMCM) to male Wistar rats. The single-day
MWM task consisted of 3 consecutive blocks of 4 trials lasting maxi-
mally 60 s each and a probe trial. During spatial learning the platform
was hidden in the middle of the NE quadrant.
Results : Two-way ANOVA with one repeated measure (block) and
animals nested in treatment has shown that latency to find the plat-
form, path efficiency and total distance travelled were on the control
level for DMCM and all doses of PWZ-029. Factors block and treat-
ment were significant only for latency to first entry to the NE quadrant
[block effect : F(2,386)=10.50, p<0.001, treatment effect : F(4,31)=3.10,
p<0.05]. Tukey’s post-hoc test revealed that animals treated with
DMCM and 5 mg/kg of PWZ-029 had longer latency to first entry
to the target quadrant than those treated with solvent (p=0.001,
p<0.001, respectively). Probe trial performance did not differ signifi-
cantly between treatments.
Conclusion : These results suggest that neither non-selective nor b5
subunit-selective negative modulation of GABA A receptors is suf-
ficient to enhance learning and short-term memory in the single-day
MWM spatial task.
PB  - Oxford Univ Press, Oxford
C3  - International Journal of Neuropsychopharmacology
T1  - Non-selective and a(5) subunit-selective negative modulators of GABA(A) receptors in a single-day morris water maze task in rats
VL  - 15
IS  - Supplement 1
SP  - 231
EP  - 231
DO  - 10.1017/S1461145712000508
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1685
ER  - 

@conference{
author = "Timić, Tamara and Divljaković, Jovana and Milinković, Marija M. and Rallapalli, Sundari and Cook, James M. and Savić, Miroslav",
year = "2012",
abstract = "Objective : It is well known that benzodiazepine binding site ligands
influence learning and memory and that the a5 subunit is significantly
involved in cognition enhancement mediated by the negative modu-
lation of GABAA receptor function. PWZ-029, a moderately selective
a5GABA A receptor inverse agonist, improved learning in passive but
not in active avoidance test, without effects on anxiety or muscle tone.
The aim of this study was to investigate effects of PWZ-029 and
DMCM, a non-selective inverse agonist, on learning ability and short-
term memory in Morris water-maze (MWM) test.
Methods : MWM test was conducted 20 minutes after in-
traperitoneal administration of treatments (solvent, 5, 15 or 30 mg/kg
PWZ-029 or 2 mg/kg DMCM) to male Wistar rats. The single-day
MWM task consisted of 3 consecutive blocks of 4 trials lasting maxi-
mally 60 s each and a probe trial. During spatial learning the platform
was hidden in the middle of the NE quadrant.
Results : Two-way ANOVA with one repeated measure (block) and
animals nested in treatment has shown that latency to find the plat-
form, path efficiency and total distance travelled were on the control
level for DMCM and all doses of PWZ-029. Factors block and treat-
ment were significant only for latency to first entry to the NE quadrant
[block effect : F(2,386)=10.50, p<0.001, treatment effect : F(4,31)=3.10,
p<0.05]. Tukey’s post-hoc test revealed that animals treated with
DMCM and 5 mg/kg of PWZ-029 had longer latency to first entry
to the target quadrant than those treated with solvent (p=0.001,
p<0.001, respectively). Probe trial performance did not differ signifi-
cantly between treatments.
Conclusion : These results suggest that neither non-selective nor b5
subunit-selective negative modulation of GABA A receptors is suf-
ficient to enhance learning and short-term memory in the single-day
MWM spatial task.",
publisher = "Oxford Univ Press, Oxford",
journal = "International Journal of Neuropsychopharmacology",
title = "Non-selective and a(5) subunit-selective negative modulators of GABA(A) receptors in a single-day morris water maze task in rats",
volume = "15",
number = "Supplement 1",
pages = "231-231",
doi = "10.1017/S1461145712000508",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1685"
}

Timić, T., Divljaković, J., Milinković, M. M., Rallapalli, S., Cook, J. M.,& Savić, M.. (2012). Non-selective and a(5) subunit-selective negative modulators of GABA(A) receptors in a single-day morris water maze task in rats. in International Journal of Neuropsychopharmacology
Oxford Univ Press, Oxford., 15(Supplement 1), 231-231.
https://doi.org/10.1017/S1461145712000508
https://hdl.handle.net/21.15107/rcub_farfar_1685

Timić T, Divljaković J, Milinković MM, Rallapalli S, Cook JM, Savić M. Non-selective and a(5) subunit-selective negative modulators of GABA(A) receptors in a single-day morris water maze task in rats. in International Journal of Neuropsychopharmacology. 2012;15(Supplement 1):231-231.
doi:10.1017/S1461145712000508
https://hdl.handle.net/21.15107/rcub_farfar_1685 .

Timić, Tamara, Divljaković, Jovana, Milinković, Marija M., Rallapalli, Sundari, Cook, James M., Savić, Miroslav, "Non-selective and a(5) subunit-selective negative modulators of GABA(A) receptors in a single-day morris water maze task in rats" in International Journal of Neuropsychopharmacology, 15, no. Supplement 1 (2012):231-231,
https://doi.org/10.1017/S1461145712000508 .,
https://hdl.handle.net/21.15107/rcub_farfar_1685 .

TY  - CONF
AU  - Joksimović, Srđan
AU  - Timić, Tamara
AU  - Radulović, Tamara
AU  - Rallapalli, Sundari
AU  - Milinković, Marija M.
AU  - Divljaković, Jovana
AU  - Batinić, Bojan
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1673
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Effects of PWZ-029, an alpha(5)GABA(A) receptor inverse agonist, on scopolamine-induced spatial learning deficits in the water maze
VL  - 22
IS  - Supplement 2
SP  - S190
EP  - S191
DO  - 10.1016/S0924-977X(12)70274-2
ER  - 

@conference{
author = "Joksimović, Srđan and Timić, Tamara and Radulović, Tamara and Rallapalli, Sundari and Milinković, Marija M. and Divljaković, Jovana and Batinić, Bojan and Cook, James M. and Savić, Miroslav",
year = "2012",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Effects of PWZ-029, an alpha(5)GABA(A) receptor inverse agonist, on scopolamine-induced spatial learning deficits in the water maze",
volume = "22",
number = "Supplement 2",
pages = "S190-S191",
doi = "10.1016/S0924-977X(12)70274-2"
}

Joksimović, S., Timić, T., Radulović, T., Rallapalli, S., Milinković, M. M., Divljaković, J., Batinić, B., Cook, J. M.,& Savić, M.. (2012). Effects of PWZ-029, an alpha(5)GABA(A) receptor inverse agonist, on scopolamine-induced spatial learning deficits in the water maze. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 22(Supplement 2), S190-S191.
https://doi.org/10.1016/S0924-977X(12)70274-2

Joksimović S, Timić T, Radulović T, Rallapalli S, Milinković MM, Divljaković J, Batinić B, Cook JM, Savić M. Effects of PWZ-029, an alpha(5)GABA(A) receptor inverse agonist, on scopolamine-induced spatial learning deficits in the water maze. in European Neuropsychopharmacology. 2012;22(Supplement 2):S190-S191.
doi:10.1016/S0924-977X(12)70274-2 .

Joksimović, Srđan, Timić, Tamara, Radulović, Tamara, Rallapalli, Sundari, Milinković, Marija M., Divljaković, Jovana, Batinić, Bojan, Cook, James M., Savić, Miroslav, "Effects of PWZ-029, an alpha(5)GABA(A) receptor inverse agonist, on scopolamine-induced spatial learning deficits in the water maze" in European Neuropsychopharmacology, 22, no. Supplement 2 (2012):S190-S191,
https://doi.org/10.1016/S0924-977X(12)70274-2 . .

TY  - JOUR
AU  - Milić, Marija
AU  - Divljaković, Jovana
AU  - Rallapalli, Sundari
AU  - van Linn, Michael
AU  - Timić, Tamara
AU  - Cook, James M.
AU  - Savić, Miroslav
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1698
AB  - Benzodiazepines negatively affect motor coordination and balance and produce myorelaxation. The aim of the present study was to examine the extent to which populations of gamma-aminobutyric acid A (GABA(A)) receptors containing alpha(1) and alpha(5) subunits contribute to these motor-impairing effects in rats. We used the nonselective agonist diazepam and the alpha(1)-selective agonist zolpidem, as well as nonselective, alpha(1)-subunit and alpha(5)-subunit-selective antagonists flumazenil, beta CCt, and XLi093, respectively. Ataxia and muscle relaxation were assessed by rotarod and grip strength tests performed 20 min after intraperitoneal treatment. Diazepam (2 mg/kg) induced significant ataxia and muscle relaxation, which were completely prevented by pretreatment with flumazenil (10mg/kg) and beta CCt (20 mg/kg). XLi093 antagonized the myorelaxant, but not the ataxic actions of diazepam. All three doses of zolpidem (1, 2, and 5 mg/kg) produced ataxia, but only the highest dose (5 mg/kg) significantly decreased the grip strength. These effects of zolpidem were reversed by beta CCt at doses of 5 and 10 mg/kg, respectively. The present study demonstrates that alpha(1) GABA(A) receptors mediate ataxia and indirectly contribute to myorelaxation in rats, whereas alpha(5) GABA(A) receptors contribute significantly, although not dominantly, to muscle relaxation but not ataxia. Behavioural Pharmacology 23:191-197
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Behavioural Pharmacology
T1  - The role of alpha(1) and alpha(5) subunit-containing GABA(A) receptors in motor impairment induced by benzodiazepines in rats
VL  - 23
IS  - 2
SP  - 191
EP  - 197
DO  - 10.1097/FBP.0b013e3283512c85
ER  - 

@article{
author = "Milić, Marija and Divljaković, Jovana and Rallapalli, Sundari and van Linn, Michael and Timić, Tamara and Cook, James M. and Savić, Miroslav",
year = "2012",
abstract = "Benzodiazepines negatively affect motor coordination and balance and produce myorelaxation. The aim of the present study was to examine the extent to which populations of gamma-aminobutyric acid A (GABA(A)) receptors containing alpha(1) and alpha(5) subunits contribute to these motor-impairing effects in rats. We used the nonselective agonist diazepam and the alpha(1)-selective agonist zolpidem, as well as nonselective, alpha(1)-subunit and alpha(5)-subunit-selective antagonists flumazenil, beta CCt, and XLi093, respectively. Ataxia and muscle relaxation were assessed by rotarod and grip strength tests performed 20 min after intraperitoneal treatment. Diazepam (2 mg/kg) induced significant ataxia and muscle relaxation, which were completely prevented by pretreatment with flumazenil (10mg/kg) and beta CCt (20 mg/kg). XLi093 antagonized the myorelaxant, but not the ataxic actions of diazepam. All three doses of zolpidem (1, 2, and 5 mg/kg) produced ataxia, but only the highest dose (5 mg/kg) significantly decreased the grip strength. These effects of zolpidem were reversed by beta CCt at doses of 5 and 10 mg/kg, respectively. The present study demonstrates that alpha(1) GABA(A) receptors mediate ataxia and indirectly contribute to myorelaxation in rats, whereas alpha(5) GABA(A) receptors contribute significantly, although not dominantly, to muscle relaxation but not ataxia. Behavioural Pharmacology 23:191-197",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Behavioural Pharmacology",
title = "The role of alpha(1) and alpha(5) subunit-containing GABA(A) receptors in motor impairment induced by benzodiazepines in rats",
volume = "23",
number = "2",
pages = "191-197",
doi = "10.1097/FBP.0b013e3283512c85"
}

Milić, M., Divljaković, J., Rallapalli, S., van Linn, M., Timić, T., Cook, J. M.,& Savić, M.. (2012). The role of alpha(1) and alpha(5) subunit-containing GABA(A) receptors in motor impairment induced by benzodiazepines in rats. in Behavioural Pharmacology
Lippincott Williams & Wilkins, Philadelphia., 23(2), 191-197.
https://doi.org/10.1097/FBP.0b013e3283512c85

Milić M, Divljaković J, Rallapalli S, van Linn M, Timić T, Cook JM, Savić M. The role of alpha(1) and alpha(5) subunit-containing GABA(A) receptors in motor impairment induced by benzodiazepines in rats. in Behavioural Pharmacology. 2012;23(2):191-197.
doi:10.1097/FBP.0b013e3283512c85 .

Milić, Marija, Divljaković, Jovana, Rallapalli, Sundari, van Linn, Michael, Timić, Tamara, Cook, James M., Savić, Miroslav, "The role of alpha(1) and alpha(5) subunit-containing GABA(A) receptors in motor impairment induced by benzodiazepines in rats" in Behavioural Pharmacology, 23, no. 2 (2012):191-197,
https://doi.org/10.1097/FBP.0b013e3283512c85 . .

TY  - JOUR
AU  - Savić, Miroslav
AU  - Milinković, Marija M.
AU  - Rallapalli, Sundari
AU  - Clayton, Terry
AU  - Joksimović, Srđan
AU  - van Linn, Michael
AU  - Cook, James M.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1176
AB  - The clinical use of benzodiazepines (BZs) is hampered by sedation and cognitive deterioration. Although genetic and pharmacological studies suggest that alpha(1)- and alpha(5)-containing GABA(A) receptors mediate and/or modulate these effects, their molecular substrate is not fully elucidated. By the use of two selective ligands: the alpha(1)-subunit affinity-selective antagonist beta-CCt, and the alpha(5)-subunit affinity- and efficacy-selective antagonist XLi093, we examined the mechanisms of behavioural effects of diazepam in the tests of spontaneous locomotor activity and water-maze acquisition and recall, the two paradigms indicative of sedative- and cognition-impairing effects of BZs, respectively. The locomotor-activity decreasing propensity of diazepam (significant at 1.5 and 5 mg/kg) was antagonized by beta-CCt (5 and 15 mg/kg), while it tended to be potentiated by XLi093 in doses of 10 mg/kg, and especially 20 mg/kg. Diazepam decreased acquisition and recall in the water maze, with a minimum effective dose of 1.5 mg/kg. Both antagonists reversed the thigmotaxis induced by 2 mg/kg diazepam throughout the test, suggesting that both GABA(A) receptor subtypes participate in BZ effects on the procedural component of the task. Diazepam-induced impairment in the declarative component of the task, as assessed by path efficiency, the latency and distance before finding the platform across acquisition trials, and also by the spatial parameters in the probe trial, was partially prevented by both, 15 mg/kg beta-CCt and 10 mg/kg XLi093. Combining a BZ with beta-CCt results in the near to control level of performance of a cognitive task, without sedation, and may be worth testing on human subjects.
PB  - Oxford Univ Press, Oxford
T2  - International Journal of Neuropsychopharmacology
T1  - The differential role of alpha(1)- and alpha(5)-containing GABA(A) receptors in mediating diazepam effects on spontaneous locomotor activity and water-maze learning and memory in rats
VL  - 12
IS  - 9
SP  - 1179
EP  - 1193
DO  - 10.1017/S1461145709000108
ER  - 

@article{
author = "Savić, Miroslav and Milinković, Marija M. and Rallapalli, Sundari and Clayton, Terry and Joksimović, Srđan and van Linn, Michael and Cook, James M.",
year = "2009",
abstract = "The clinical use of benzodiazepines (BZs) is hampered by sedation and cognitive deterioration. Although genetic and pharmacological studies suggest that alpha(1)- and alpha(5)-containing GABA(A) receptors mediate and/or modulate these effects, their molecular substrate is not fully elucidated. By the use of two selective ligands: the alpha(1)-subunit affinity-selective antagonist beta-CCt, and the alpha(5)-subunit affinity- and efficacy-selective antagonist XLi093, we examined the mechanisms of behavioural effects of diazepam in the tests of spontaneous locomotor activity and water-maze acquisition and recall, the two paradigms indicative of sedative- and cognition-impairing effects of BZs, respectively. The locomotor-activity decreasing propensity of diazepam (significant at 1.5 and 5 mg/kg) was antagonized by beta-CCt (5 and 15 mg/kg), while it tended to be potentiated by XLi093 in doses of 10 mg/kg, and especially 20 mg/kg. Diazepam decreased acquisition and recall in the water maze, with a minimum effective dose of 1.5 mg/kg. Both antagonists reversed the thigmotaxis induced by 2 mg/kg diazepam throughout the test, suggesting that both GABA(A) receptor subtypes participate in BZ effects on the procedural component of the task. Diazepam-induced impairment in the declarative component of the task, as assessed by path efficiency, the latency and distance before finding the platform across acquisition trials, and also by the spatial parameters in the probe trial, was partially prevented by both, 15 mg/kg beta-CCt and 10 mg/kg XLi093. Combining a BZ with beta-CCt results in the near to control level of performance of a cognitive task, without sedation, and may be worth testing on human subjects.",
publisher = "Oxford Univ Press, Oxford",
journal = "International Journal of Neuropsychopharmacology",
title = "The differential role of alpha(1)- and alpha(5)-containing GABA(A) receptors in mediating diazepam effects on spontaneous locomotor activity and water-maze learning and memory in rats",
volume = "12",
number = "9",
pages = "1179-1193",
doi = "10.1017/S1461145709000108"
}

Savić, M., Milinković, M. M., Rallapalli, S., Clayton, T., Joksimović, S., van Linn, M.,& Cook, J. M.. (2009). The differential role of alpha(1)- and alpha(5)-containing GABA(A) receptors in mediating diazepam effects on spontaneous locomotor activity and water-maze learning and memory in rats. in International Journal of Neuropsychopharmacology
Oxford Univ Press, Oxford., 12(9), 1179-1193.
https://doi.org/10.1017/S1461145709000108

Savić M, Milinković MM, Rallapalli S, Clayton T, Joksimović S, van Linn M, Cook JM. The differential role of alpha(1)- and alpha(5)-containing GABA(A) receptors in mediating diazepam effects on spontaneous locomotor activity and water-maze learning and memory in rats. in International Journal of Neuropsychopharmacology. 2009;12(9):1179-1193.
doi:10.1017/S1461145709000108 .

Savić, Miroslav, Milinković, Marija M., Rallapalli, Sundari, Clayton, Terry, Joksimović, Srđan, van Linn, Michael, Cook, James M., "The differential role of alpha(1)- and alpha(5)-containing GABA(A) receptors in mediating diazepam effects on spontaneous locomotor activity and water-maze learning and memory in rats" in International Journal of Neuropsychopharmacology, 12, no. 9 (2009):1179-1193,
https://doi.org/10.1017/S1461145709000108 . .

TY  - CONF
AU  - Milinković, Marija M.
AU  - Savić, Miroslav
AU  - Rallapalli, Sundari
AU  - Samardžić, Janko
AU  - van Linn, Michael
AU  - Ugrešić, Nenad
AU  - Cook, James M.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1063
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - RY-023, an inverse agonist at alpha5 GABAA receptors: the influence on spatial memory and spontaneous locomotor activity
VL  - 18
IS  - Supplement 4
SP  - S284
EP  - S284
DO  - 10.1016/S0924-977X(08)70375-4
ER  - 

@conference{
author = "Milinković, Marija M. and Savić, Miroslav and Rallapalli, Sundari and Samardžić, Janko and van Linn, Michael and Ugrešić, Nenad and Cook, James M.",
year = "2008",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "RY-023, an inverse agonist at alpha5 GABAA receptors: the influence on spatial memory and spontaneous locomotor activity",
volume = "18",
number = "Supplement 4",
pages = "S284-S284",
doi = "10.1016/S0924-977X(08)70375-4"
}

Milinković, M. M., Savić, M., Rallapalli, S., Samardžić, J., van Linn, M., Ugrešić, N.,& Cook, J. M.. (2008). RY-023, an inverse agonist at alpha5 GABAA receptors: the influence on spatial memory and spontaneous locomotor activity. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 18(Supplement 4), S284-S284.
https://doi.org/10.1016/S0924-977X(08)70375-4

Milinković MM, Savić M, Rallapalli S, Samardžić J, van Linn M, Ugrešić N, Cook JM. RY-023, an inverse agonist at alpha5 GABAA receptors: the influence on spatial memory and spontaneous locomotor activity. in European Neuropsychopharmacology. 2008;18(Supplement 4):S284-S284.
doi:10.1016/S0924-977X(08)70375-4 .

Milinković, Marija M., Savić, Miroslav, Rallapalli, Sundari, Samardžić, Janko, van Linn, Michael, Ugrešić, Nenad, Cook, James M., "RY-023, an inverse agonist at alpha5 GABAA receptors: the influence on spatial memory and spontaneous locomotor activity" in European Neuropsychopharmacology, 18, no. Supplement 4 (2008):S284-S284,
https://doi.org/10.1016/S0924-977X(08)70375-4 . .

TY  - CONF
AU  - Samardžić, Janko
AU  - Savić, Miroslav
AU  - Clayton, Terry
AU  - Rallapalli, Sundari
AU  - Obradović, Dragan I.
AU  - Joksimović, Srđan
AU  - Sieghart, Werner
AU  - Cook, James M.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1025
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Effects of a novel inverse agonist selective for alpha5 GABAA receptors on spatial memory: comparison with the standard non-selective inverse agonist DMCM
VL  - 18
IS  - Supplement 4
SP  - S285
EP  - S285
DO  - 10.1016/S0924-977X(08)70377-8
ER  - 

@conference{
author = "Samardžić, Janko and Savić, Miroslav and Clayton, Terry and Rallapalli, Sundari and Obradović, Dragan I. and Joksimović, Srđan and Sieghart, Werner and Cook, James M.",
year = "2008",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Effects of a novel inverse agonist selective for alpha5 GABAA receptors on spatial memory: comparison with the standard non-selective inverse agonist DMCM",
volume = "18",
number = "Supplement 4",
pages = "S285-S285",
doi = "10.1016/S0924-977X(08)70377-8"
}

Samardžić, J., Savić, M., Clayton, T., Rallapalli, S., Obradović, D. I., Joksimović, S., Sieghart, W.,& Cook, J. M.. (2008). Effects of a novel inverse agonist selective for alpha5 GABAA receptors on spatial memory: comparison with the standard non-selective inverse agonist DMCM. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 18(Supplement 4), S285-S285.
https://doi.org/10.1016/S0924-977X(08)70377-8

Samardžić J, Savić M, Clayton T, Rallapalli S, Obradović DI, Joksimović S, Sieghart W, Cook JM. Effects of a novel inverse agonist selective for alpha5 GABAA receptors on spatial memory: comparison with the standard non-selective inverse agonist DMCM. in European Neuropsychopharmacology. 2008;18(Supplement 4):S285-S285.
doi:10.1016/S0924-977X(08)70377-8 .

Samardžić, Janko, Savić, Miroslav, Clayton, Terry, Rallapalli, Sundari, Obradović, Dragan I., Joksimović, Srđan, Sieghart, Werner, Cook, James M., "Effects of a novel inverse agonist selective for alpha5 GABAA receptors on spatial memory: comparison with the standard non-selective inverse agonist DMCM" in European Neuropsychopharmacology, 18, no. Supplement 4 (2008):S285-S285,
https://doi.org/10.1016/S0924-977X(08)70377-8 . .

TY  - CONF
AU  - Savić, Miroslav
AU  - Rallapalli, Sundari
AU  - Milinković, Marija M.
AU  - Samardžić, Janko
AU  - van Linn, Michael
AU  - Cook, James M.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1102
PB  - Cambridge Univ Press, New York
C3  - International Journal of Neuropsychopharmacology
T1  - Hypolocomotor activity of DIAZEPAM in wistar rats is mediated by Gabaa receptors containing the Alphal, but not the Alpha5 subunit
VL  - 11
SP  - 212
EP  - 212
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1102
ER  - 

@conference{
author = "Savić, Miroslav and Rallapalli, Sundari and Milinković, Marija M. and Samardžić, Janko and van Linn, Michael and Cook, James M.",
year = "2008",
publisher = "Cambridge Univ Press, New York",
journal = "International Journal of Neuropsychopharmacology",
title = "Hypolocomotor activity of DIAZEPAM in wistar rats is mediated by Gabaa receptors containing the Alphal, but not the Alpha5 subunit",
volume = "11",
pages = "212-212",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1102"
}

Savić, M., Rallapalli, S., Milinković, M. M., Samardžić, J., van Linn, M.,& Cook, J. M.. (2008). Hypolocomotor activity of DIAZEPAM in wistar rats is mediated by Gabaa receptors containing the Alphal, but not the Alpha5 subunit. in International Journal of Neuropsychopharmacology
Cambridge Univ Press, New York., 11, 212-212.
https://hdl.handle.net/21.15107/rcub_farfar_1102

Savić M, Rallapalli S, Milinković MM, Samardžić J, van Linn M, Cook JM. Hypolocomotor activity of DIAZEPAM in wistar rats is mediated by Gabaa receptors containing the Alphal, but not the Alpha5 subunit. in International Journal of Neuropsychopharmacology. 2008;11:212-212.
https://hdl.handle.net/21.15107/rcub_farfar_1102 .

Savić, Miroslav, Rallapalli, Sundari, Milinković, Marija M., Samardžić, Janko, van Linn, Michael, Cook, James M., "Hypolocomotor activity of DIAZEPAM in wistar rats is mediated by Gabaa receptors containing the Alphal, but not the Alpha5 subunit" in International Journal of Neuropsychopharmacology, 11 (2008):212-212,
https://hdl.handle.net/21.15107/rcub_farfar_1102 .