TY  - JOUR
AU  - Stanković, Milena N.
AU  - Mladenović, Dušan
AU  - Ninković, Milica
AU  - Đuričić, Ivana
AU  - Šobajić, Slađana
AU  - Jorgacević, Bojan
AU  - de Luka, Silvio
AU  - Jesić-Vukicević, Rada
AU  - Radosavljević, Tatjana S.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2176
AB  - Development of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by increasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD
VL  - 17
IS  - 2
SP  - 254
EP  - 261
DO  - 10.1089/jmf.2013.0111
ER  - 

@article{
author = "Stanković, Milena N. and Mladenović, Dušan and Ninković, Milica and Đuričić, Ivana and Šobajić, Slađana and Jorgacević, Bojan and de Luka, Silvio and Jesić-Vukicević, Rada and Radosavljević, Tatjana S.",
year = "2014",
abstract = "Development of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by increasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD",
volume = "17",
number = "2",
pages = "254-261",
doi = "10.1089/jmf.2013.0111"
}

Stanković, M. N., Mladenović, D., Ninković, M., Đuričić, I., Šobajić, S., Jorgacević, B., de Luka, S., Jesić-Vukicević, R.,& Radosavljević, T. S.. (2014). The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 17(2), 254-261.
https://doi.org/10.1089/jmf.2013.0111

Stanković MN, Mladenović D, Ninković M, Đuričić I, Šobajić S, Jorgacević B, de Luka S, Jesić-Vukicević R, Radosavljević TS. The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD. in Journal of Medicinal Food. 2014;17(2):254-261.
doi:10.1089/jmf.2013.0111 .

Stanković, Milena N., Mladenović, Dušan, Ninković, Milica, Đuričić, Ivana, Šobajić, Slađana, Jorgacević, Bojan, de Luka, Silvio, Jesić-Vukicević, Rada, Radosavljević, Tatjana S., "The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD" in Journal of Medicinal Food, 17, no. 2 (2014):254-261,
https://doi.org/10.1089/jmf.2013.0111 . .