TY  - JOUR
AU  - Milićević, Zorka
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Kasapović, Jelena
AU  - Anđelković, Uroš
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2169
AB  - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
PB  - Hindawi Publishing Corp, New York
T2  - Sensors
T1  - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
DO  - 10.1155/2014/618698
ER  - 

@article{
author = "Milićević, Zorka and Bajić, Vladan and Živković, Lada and Kasapović, Jelena and Anđelković, Uroš and Potparević, Biljana",
year = "2014",
abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Sensors",
title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells",
doi = "10.1155/2014/618698"
}

Milićević, Z., Bajić, V., Živković, L., Kasapović, J., Anđelković, U.,& Potparević, B.. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Sensors
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/618698

Milićević Z, Bajić V, Živković L, Kasapović J, Anđelković U, Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Sensors. 2014;.
doi:10.1155/2014/618698 .

Milićević, Zorka, Bajić, Vladan, Živković, Lada, Kasapović, Jelena, Anđelković, Uroš, Potparević, Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" in Sensors (2014),
https://doi.org/10.1155/2014/618698 . .

TY  - JOUR
AU  - Milicević, Zorka
AU  - Kasapović, Jelena
AU  - Gavrilović, Ljubica
AU  - Milovanović, Zorka
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2096
AB  - It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.
PB  - Excli Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Mutant p53 protein expression and antioxidant status deficiency in breast cancer
VL  - 13
SP  - 691
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_vinar_113
ER  - 

@article{
author = "Milicević, Zorka and Kasapović, Jelena and Gavrilović, Ljubica and Milovanović, Zorka and Bajić, Vladan and Potparević, Biljana",
year = "2014",
abstract = "It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.",
publisher = "Excli Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Mutant p53 protein expression and antioxidant status deficiency in breast cancer",
volume = "13",
pages = "691-708",
url = "https://hdl.handle.net/21.15107/rcub_vinar_113"
}

Milicević, Z., Kasapović, J., Gavrilović, L., Milovanović, Z., Bajić, V.,& Potparević, B.. (2014). Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal
Excli Journal Managing Office, Dortmund., 13, 691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113

Milicević Z, Kasapović J, Gavrilović L, Milovanović Z, Bajić V, Potparević B. Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal. 2014;13:691-708.
https://hdl.handle.net/21.15107/rcub_vinar_113 .

Milicević, Zorka, Kasapović, Jelena, Gavrilović, Ljubica, Milovanović, Zorka, Bajić, Vladan, Potparević, Biljana, "Mutant p53 protein expression and antioxidant status deficiency in breast cancer" in EXCLI Journal, 13 (2014):691-708,
https://hdl.handle.net/21.15107/rcub_vinar_113 .